Patents Assigned to Sangamo Biosciences, Inc.
  • Targeted integration into the locus
    Patent number: 8329986
    Abstract: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into a plant Zp15 locus, for example, for expression of a polypeptide of interest.
    Type: Grant
    Filed: December 17, 2009
    Date of Patent: December 11, 2012
    Assignees: Dow AgroSciences, LLC, Sangamo BioSciences, Inc.
    Inventors: Holly J. Butler, David R. Corbin, Yannick Doyon, Zhifang Gao, Vipula K. Shukla, Fyodor Urnov, Sarah E. Worden
  • Zinc finger proteins and method for inactivating a dhfr gene in a chinese hamster ovary cell
    Patent number: 8313925
    Abstract: Disclosed herein are methods and compositions for targeted integration of a exogenous sequence into a predetermined target site in a genome for use, for example, in protein expression and gene inactivation.
    Type: Grant
    Filed: March 9, 2009
    Date of Patent: November 20, 2012
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Philip D. Gregory, Jeffrey C. Miller
  • Repressing endogenous CCR5 gene expression in cells using engineered zinc finger proteins
    Patent number: 8268618
    Abstract: The present invention provides methods for modulating expression of endogenous cellular genes using recombinant zinc finger proteins.
    Type: Grant
    Filed: August 16, 2006
    Date of Patent: September 18, 2012
    Assignee: Sangamo BioSciences, Inc.
    Inventors: George Norbert Cox, III, Casey Christopher Case, Stephen P. Eisenberg, Eric Edward Jarvis, Sharon Kaye Spratt
  • Methods and compositions for inactivating glutamine synthetase gene expression
    Patent number: 8153399
    Abstract: Disclosed herein are methods and compositions for inactivating a glutamine synthetase (GS) gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
    Type: Grant
    Filed: October 29, 2009
    Date of Patent: April 10, 2012
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Pei-Qi Liu, Jeffrey C. Miller
  • Targeted integration into the PPP1R12C locus
    Patent number: 8110379
    Abstract: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.
    Type: Grant
    Filed: April 24, 2008
    Date of Patent: February 7, 2012
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Russell DeKelver, Philip D. Gregory, David Paschon, Phillip Tam, Fyodor Urnov
  • METHODS AND COMPOSITIONS FOR TREATING NEUROPATHIC PAIN
    Publication number: 20110306559
    Abstract: Compositions and methods for the treatment of neuropathic pain are provided. Compositions of the invention may comprise proteins with a zinc-finger domain fused to a regulatory domain that is capable of either activating or repressing the expression of a target gene involved in neuropathic pain. Alternatively, compositions of the invention may comprise a nucleic acid sequence encoding a protein of the invention, which nucleic acid sequence may optionally be provided as a plasmid or within a virus or other vector for delivery to a target cell or tissue. Methods of treating neuropathic pain involving treatment of subject with the compositions of the invention are also provided. Exemplary target genes for the treatment of neuropathic pain include VR1, NaV1.8, and TrkA.
    Type: Application
    Filed: December 23, 2009
    Publication date: December 15, 2011
    Applicant: SANGAMO BIOSCIENCES, INC.
    Inventors: John R.M. Forsayeth, Raymond A. Chavez, Trevor N. Collingwood
  • Regulation of angiogenesis with zinc finger proteins
    Patent number: 8071564
    Abstract: Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.
    Type: Grant
    Filed: September 3, 2009
    Date of Patent: December 6, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Edward Rebar, Andrew Jamieson, Qiang Liu, Pei-Qi Liu, Alan Wolffe, Stephen P. Eisenberg, Eric Jarvis
  • Targeted modification of chromatin structure
    Patent number: 8071370
    Abstract: Methods and compositions for targeted modification of chromatin structure, within a region of interest in cellular chromatin, are provided. Such methods and compositions are useful for facilitating processes such as, for example, transcription and recombination, that require access of exogenous molecules to chromosomal DNA sequences.
    Type: Grant
    Filed: July 9, 2010
    Date of Patent: December 6, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Alan P. Wolffe, Elizabeth J. Wolffe, legal representative, Trevor Collingwood, Philip D. Gregory, Andrew Snowden, Fyodor Urnov
  • Engineered cleavage half-domains
    Patent number: 8034598
    Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
    Type: Grant
    Filed: June 23, 2009
    Date of Patent: October 11, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventor: Jeffrey C. Miller
  • Anti-angiogenic methods and compositions
    Patent number: 8012946
    Abstract: Disclosed herein are methods and compositions for treatment of conditions requiring inhibition of angiogenesis. Such conditions include those characterized by neovascularization, such as retinopathies, macular degeneration and various malignancies.
    Type: Grant
    Filed: January 9, 2008
    Date of Patent: September 6, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: H. Steve Zhang, Philip D. Gregory, Edward J. Rebar
  • Regulation of endogenous gene expression in cells using zinc finger proteins
    Patent number: 7985887
    Abstract: The present invention provides methods for modulating expression of endogenous cellular genes using recombinant zinc finger proteins.
    Type: Grant
    Filed: August 17, 2006
    Date of Patent: July 26, 2011
    Assignee: Sangamo BioSciences, Inc.
    Inventors: George Norbert Cox, III, Casey Christopher Case, Stephen P. Eisenberg, Eric Edward Jarvis, Sharon Kaye Spratt
  • Methods and compositions for targeted cleavage and recombination
    Patent number: 7972854
    Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region.
    Type: Grant
    Filed: February 3, 2005
    Date of Patent: July 5, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Jeffrey C. Miller, Lei Zhang
  • Methods and compositions for gene inactivation
    Patent number: 7951925
    Abstract: Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
    Type: Grant
    Filed: May 23, 2007
    Date of Patent: May 31, 2011
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Dale Ando, Michael C. Holmes, Gary Ka Leong Lee
  • Compositions and methods for regulation of plant gamma-tocopherol methyltransferase
    Patent number: 7947873
    Abstract: Disclosed herein are zinc finger proteins that bind to target sites in a plant gamma-tocopherol methyl transferase (GMT) gene; compositions comprising these GMT-targeted zinc finger proteins and methods of making and using such zinc finger proteins. Also disclosed are methods for modulating the alpha-tocopherol content in various plant organs, particularly seeds, in transgenic plants.
    Type: Grant
    Filed: April 17, 2003
    Date of Patent: May 24, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Guofu Li, Qiang Liu, Andrew Jamieson, Edward Rebar, Mylavarapu Venkatramesh
  • Randomized libraries of zinc finger proteins
    Patent number: 7943553
    Abstract: The present invention relates to methods of using libraries of randomized zinc finger proteins to identify genes associated with selected phenotypes.
    Type: Grant
    Filed: July 12, 2006
    Date of Patent: May 17, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Casey C. Case, Edward J. Rebar, Andrew Jamieson
  • Simultaneous modulation of multiple genes
    Patent number: 7939327
    Abstract: Disclosed herein are compositions and methods that regulate expression of two or more endogenous genes.
    Type: Grant
    Filed: February 28, 2008
    Date of Patent: May 10, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Jeffrey Miller, Guofu Li, Carl Pabo, Trevor Collingwood
  • Libraries of regulatory sequences, methods of making and using same
    Patent number: 7923542
    Abstract: Methods and compositions for the identification, isolation and characterization of regulatory DNA sequences in a cell of interest are provided. In particular, libraries of regulatory sequences are provided, in which each member of the library comprises a polynucleotide comprising sequences from an accessible region of cellular chromatin.
    Type: Grant
    Filed: October 24, 2001
    Date of Patent: April 12, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Alan P. Wolffe, Elizabeth J. Wolffe, legal representative, Fyodor Urnov
  • Methods and compositions for inactivating alpha 1,6 fucosyltransferase () gene expression
    Patent number: 7919313
    Abstract: Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
    Type: Grant
    Filed: July 10, 2008
    Date of Patent: April 5, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Trevor Collingwood, Gregory J. Cost
  • Engineered cleavage half-domains
    Patent number: 7914796
    Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising these polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
    Type: Grant
    Filed: July 2, 2008
    Date of Patent: March 29, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: Jeffrey C. Miller, Michael C. Holmes, Gregory J. Cost
  • Anti-angiogenic methods and compositions
    Patent number: 7893022
    Abstract: Disclosed herein are methods and compositions for treatment of conditions requiring inhibition of angiogenesis. Such conditions include those characterized by neovascularization, such as retinopathies, macular degeneration and various malignancies.
    Type: Grant
    Filed: October 31, 2007
    Date of Patent: February 22, 2011
    Assignee: Sangamo Biosciences, Inc.
    Inventors: H. Steve Zhang, Philip D. Gregory, Edward J. Rebar