Abstract: An object of the invention is to provide a primer for detecting and quantifying homozygous deletion of the SMN1 gene, the deletion of which causes SMA, using a dried blood spot in a filter paper and a method related to specific detection/quantification of the SMN1 gene using the primer.

Abstract: Provided is a composition for mononuclear cell-containing plasma separation capable of suppressing a flow of the composition for mononuclear cell-containing plasma separation during storage, capable of satisfactorily forming a partition during centrifugation, and capable of yielding mononuclear cell-containing plasma with little admixture of blood cell components other than mononuclear cells. The composition for mononuclear cell-containing plasma separation according to the present invention contains an organic component having fluidity at 25° C. and two or more kinds of inorganic fine powders, and has a specific gravity of 1.060 or more and 1.080 or less at 25° C.

Abstract: The invention aims to provide an immunochromatographic assay method in which the non-specific reaction is suppressed, and a test strip to be used in the method. The object can be achieved by an immunochromatographic assay method for an analyte, the method including using a test strip including the following (1) to (3): (1) a sample pad containing a sample application region for applying a biological sample that may contain the analyte; (2) an insoluble membrane containing at least one detection region to which a binding partner immunologically reactive with the analyte is immobilized; and (3) a conjugate containing a binding partner immunologically reactive with the analyte, the binding partner being immobilized to a label; characterized in that the conjugate is retained in a conjugate immobilization region together with an anionic surfactant.

Abstract: Disclosed is a sensitized magnetic responsive particle including: a magnetic responsive particle having a core particle and at least one magnetic layer that is disposed on the core particle and includes microparticles of a magnetic metal and/or an oxide thereof; and a substance that is supported on the magnetic responsive Particle and interacts specifically with an analyte, wherein the coefficient of variation in the weight-average particle size of the magnetic responsive particles is 15% or less. The magnetic responsive particle provided has excellent magnetic separability.

Abstract: The problem to be solved by the present invention is to provide a reagent that can reduce deposition of fouling in the reaction cell, by using an existing reaction cell, without affecting the composition of the reagent, in an immunoassay method using a reagent containing latex particles; and to provide an immunoassay method using the reagent. Provided is an immunoassay reagent including a latex particle, wherein the latex particle contains a halogen atom. Also provided is an immunoassay method including contacting a latex particle with a sample to be measured in a reaction cell, wherein the latex particle contains a halogen atom.

Abstract: The invention aims to suppress non-specific reaction effectively in an immunochromatographic assay method for an analyte. The object can be achieved by an immunochromatographic assay method for an analyte, the method including using a test strip including the following (1) to (3): (1) a sample pad containing a sample application region for applying a biological sample that may contain the analyte; (2) an insoluble membrane containing at least one detection region to which a binding partner immunologically reactive with the analyte is immobilized, wherein the binding partner is immobilized to a label; and (3) a conjugate containing a binding partner immunologically reactive with the analyte, wherein the binding partner is immobilized to a label.

Abstract: The invention aims to carry out an immunoassay method for a respiratory tract virus, wherein non-specific reaction is suppressed. The object can be achieved by an immunoassay method for a respiratory tract virus, the method comprising: (A) bringing a biological sample that may contain a respiratory tract virus into contact with an anionic surfactant; (B) bringing the respiratory tract virus into contact with a conjugate, to forma first complex; (C) bringing the first complex into contact with an antibody immunologically reactive with the respiratory tract virus, to forma second complex; and (D) measuring a signal derived from the conjugate.

Abstract: A problem to be solved is to perform an immunoassay method for BG in a biological sample having a sensitivity equivalent to and a reactivity similar to a Limulus amebocyte lysate reagent. The problem can be solved by an immunoassay method for ?-D-glucan in a biological sample, comprising assaying ?-D-glucan in the biological sample by using a monoclonal antibody binding to (1?3)-?-D-glucan having a degree of polymerization of 4.

Abstract: To provide a method for analyzing coagulation characteristics of a blood specimen. To provide a method for analyzing a blood specimen, including: acquiring a waveform related to a coagulation rate or coagulation acceleration of a sample obtained by mixing a subject blood specimen with a reagent for measuring coagulation time; extracting multiple parameters characterizing the waveform related to a coagulation rate or a coagulation acceleration; and determining an activity level or activity abnormality of a coagulation factor in the subject blood specimen on the basis of the multiple parameters.

Abstract: A problem to be solved is to detect only free AIM in a biological sample containing complex AIM and free AIM. The problem can be solved by an immunoassay method for free AIM in a biological sample using an anti-AIM monoclonal antibody having a property of reacting with free AIM and not reacting with complex AIM, wherein the anti-AIM monoclonal antibody is an antibody binding to an epitope in the SRCR2 domain of human AIM.

Abstract: Provided is a clot adhesion preventing agent capable of suppressing adhesion of clot to the inner wall surface of a blood collection container. The clot adhesion preventing agent according to the present invention includes a polyether compound or a silicone oil, and an amino acid.

Abstract: An object is to provide a leucine-rich ?2 glycoprotein composition comprising leucine-rich ?2 glycoprotein and having excellent preservation stability. The object can be achieved by a leucine-rich ?2 glycoprotein composition comprising leucine-rich ?2 glycoprotein and having a pH of 7.0 to 9.3.

Abstract: A problem to be solved is to further improve the specificity for free AIM of an antibody specifically reacting with free AIM and to diagnose NASH without imposing a burden on patients and medical staffs. The problem can be solved by an immunoassay method for free AIM in a biological sample containing complex AIM and free AIM, and the method comprises bringing the biological sample into contact with an antibody specifically reacting with free AIM in a presence of an anti-IgM antibody.

Abstract: A problem to be solved is to improve the specificity of an anti-AIM antibody for free AIM in a biological sample containing complex AIM and free AIM. The problem can be solved by an immunoassay method for measuring an amount of free AIM in a biological sample containing complex AIM and free AIM, and the method comprises bringing the biological sample into contact with an anti-AIM antibody in the presence of an anti-IgM antibody.

Abstract: Provided is a nucleic acid isolation method whereby it becomes possible to extract a nucleic acid in a simple manner without the need to use an alcohol. A nucleic acid isolation method, comprising the steps of: mixing a specimen containing a nucleic acid with an extraction solution to disperse the nucleic acid in the extraction solution; bringing the extraction solution containing the nucleic acid into contact with an anionic adsorbent; bringing a washing solution into contact with the anionic adsorbent; and bringing a collection solution into contact with the anionic adsorbent, the extraction solution containing a protein denaturant, the washing solution containing a basic compound and having a pH value equal to or less than a pKa value of a conjugate acid of the basic compound, and the collection solution having a pH value equal to or more than the pKa value of the conjugate acid of the basic compound.

Abstract: A problem of the present invention is to provide an immunochromatographic test strip avoiding agglutination of colloidal gold while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a hemagglutinating agent and the colloidal gold conjugates as a detection reagent, and to provide immunochromatography using the test strip. To solve the problem, the present inventors reviewed the composition of the existing reagent itself from a completely different viewpoint rather than the selection of type or amount of polyanions, and as a result of extensive study on each element, the inventors surprisingly found that agglutination of colloidal gold may be suppressed by using a particular additive without neutralization by polyanions.

Abstract: A testing vessel 1 includes a flexible vessel body 10 having a bottom and a hollow shape; and a partition 11 axially extending in the vessel body 10 and dividing an analyte extract containable space 50 in the vessel body 10 into two or more compartments. The testing vessel 1 enables two or more items to be readily tested with two or more test pieces.

Abstract: The present invention provides a method of detecting and a method of quantifying oligonucleotides with more excellent specificity and quantitativity as compared to conventional signal amplification (PALSAR) measurement methods. In the present invention, the problem is solved by hybridizing a target oligonucleotide to be measured with a complementary nucleic acid probe (3?-complementary sequence of target sequence-5?), or hybridizing the target oligonucleotide to be measured having given bases such as poly(A) added thereto with a complementary nucleic acid probe (3?-complementary sequence of target oligonucleotide+complementary sequence of given bases-5?), decomposing and removing an incomplete hybridization product by using a single-strand-specific nuclease such as Si nuclease, and measuring the nucleic acid probe contained in a remaining complete hybridization product by a PALSAR method.

Abstract: Provided are a method for determining prognosis of endometrial cancer, a method for determining endometrial cancer patient compatible to preservation therapy, and a method for determining risk of endometrial cancer. The methods comprise detecting DNA methylation level at a target CpG site in a genomic DNA derived from an endometrial cell, endometrial cancer cell or tissue containing the cell. Prognosis of endometrial cancer, compatibility to preservation therapy, or risk of endometrial cancer of a subject is determined on the basis of the detected DNA methylation level.

Abstract: A carbon isotope analysis method, including the steps of: generating carbon dioxide isotope from carbon isotope; feeding the carbon dioxide isotope into an optical resonator having a pair of mirrors; applying irradiation light having an absorption wavelength of the carbon dioxide isotope into the optical resonator; adjusting a relative positional relationship between the mirrors so that an optical axis of the irradiation light and an optical axis of light generated by the etalon effect are not matched; measuring the intensity of the transmitted light generated by resonance of carbon dioxide isotope excited by the irradiation light; and calculating the concentration of the carbon isotope from the intensity of the transmitted light. An optical resonator that can be suppressed in the parasitic etalon effect, and a carbon isotope analysis device and a carbon isotope analysis method, by use of the optical resonator, are provided.