Abstract: The present disclosure provides improved nucleic acid sequencing-by-synthesis (SBS) methods, related kits and reagents, and systems for performing such methods using such kits and reagents.
Type:
Grant
Filed:
March 6, 2018
Date of Patent:
February 28, 2023
Assignee:
SINGULAR GENOMICS SYSTEMS, INC.
Inventors:
Eli Glezer, Andrew Spaventa, David Barker
Abstract: Disclosed herein, inter alia, are substrates, kits, and efficient methods of preparing and sequencing two or more regions of a double-stranded polynucleotide.
Type:
Grant
Filed:
June 14, 2022
Date of Patent:
January 24, 2023
Assignee:
SINGULAR GENOMICS SYSTEMS, INC.
Inventors:
Daan Witters, Eli N. Glezer, Allen Lipson
Abstract: Provided herein are methods including alternating series of sequencing cycles and dark extension cycles allowing longer read lengths and addressing disadvantages of traditional nucleic acid sequencing protocols.
Type:
Grant
Filed:
December 18, 2020
Date of Patent:
January 18, 2022
Assignee:
SINGULAR GENOMICS SYSTEMS, INC.
Inventors:
Eli N. Glezer, Abrehet Abdu, Timothy Looney
Abstract: Provided herein are methods of making, amplifying, and sequencing tagged nucleic acid complements, compositions including interposing oligonucleotide barcodes, and kits useful in obtaining long-range sequence data.
Type:
Grant
Filed:
December 18, 2020
Date of Patent:
October 26, 2021
Assignee:
SINGULAR GENOMICS SYSTEMS, INC.
Inventors:
Eli N. Glezer, Martin Maria Fabani, Ryan Shultzaberger, Bharat Sridhar, Gudrun Stengel, Christopher Jen-Yue Wei
Abstract: Provided herein are modified Archaeal family B polymerases derived from the Archaeal microorganism Pyrococcus abyssi that exhibit improved incorporation of nucleotide analogues utilized in DNA sequencing.
Type:
Grant
Filed:
September 11, 2019
Date of Patent:
October 5, 2021
Assignee:
SINGULAR GENOMICS SYSTEMS, INC.
Inventors:
Souad Naji, Carl W. Fuller, Eli Glezer, Andrew Spaventa