Abstract: Markers of acute myeloid leukemia stem cells (AMLSC) are identified. The markers are differentially expressed in comparison with normal counterpart cells, and are useful as diagnostic and therapeutic targets.

Abstract: The present invention concerns the enhancement of the osteogenic potential of bone graft by ex vivo treatment with a Wnt polypeptide, such as a liposomal Wnt polypeptide.

Abstract: The present disclosure provides systems, compositions and methods for regulating expression of a target polynucleotide in a cell. The systems, compositions and methods comprise a chimeric receptor polypeptide comprising a G-protein coupled receptor (GPCR) or a fragment thereof, a chimeric adaptor polypeptide, at least one actuator moiety and a cleavage moiety.

Abstract: Compounds, compositions and methods are provided for the inhibition of GBP1. The subject inhibitor compounds can act by inhibiting GBP1 alone and/or GBP1: pro-survival kinase (e.g. serine/threonine-protein kinase pim-1 (PIM1)) interactions. Aspects of the subject methods include contacting a cellular sample with a GBP 1 inhibitor to inhibit the GBP 1 alone and/or GBP 1: PIM 1 interactions. Also provided are compositions and methods for treating cancer. In certain cases the cancer is resistant towards chemotherapy and radiation therapy.

Abstract: Systems and methods for tracking objects are disclosed herein. In one embodiment, a system having a processor merges features of detected objects extracted from a point cloud and a corresponding image to generate fused features for the detected objects, generates a learned distance metric for the detected objects using the fused features, determines matched detected objects and unmatched detected objects, applies prior tracking identifiers of the detected objects at the prior time to the matched detected objects, determines a confidence score for the fused features of the unmatched detected objects, and applies new tracking identifiers to the unmatched detected objects based on the confidence score.

Abstract: Methods of detecting diagnostic biomarkers on cancer-derived extracellular vesicles such as exosomes by mid-infrared spectroscopy are provided. In particular, methods of detecting ovarian cancer by spectral fingerprint analysis of molecular vibrational spectroscopic markers on individual or subpopulations of extracellular vesicles are disclosed.

Abstract: Systems and methods for crowdsourced machine learning in accordance with embodiments of the invention are illustrated. In many embodiments, particular crowdworkers from a plurality of crowdworkers who are able to perform with high accuracy and reliability (referred to herein as “super recognizers”) are identified and used to generate training data for machine learning models. In various embodiments, super recognizers are identified by providing a request to answer questions regarding a particular type of input to the plurality of crowdworkers and providing received answers to a machine learning model trained using expert-annotated inputs similar to the inputs provided to the crowdworkers.

Abstract: Systems and methods for automated identification of STEMI in accordance with embodiments of the invention are illustrated. One embodiment includes a predictive electronic health record (EHR) system, including a processor, and a memory, the memory containing an EHR management application which configures the processor to obtain preliminary information about a patient via a terminal, store the preliminary information in an EHR, provide the preliminary information to a model trained to predict an emergency medical condition requiring immediate treatment, obtain a likelihood that the patient is suffering from an emergency medical condition from the machine learning model, and provide an alert when the likelihood exceeds a predetermined threshold. In many embodiments, the condition is STEMI, and the preliminary information comprises: age, sex, and at least one chief complaint.

Abstract: System, methods, and other embodiments described herein relate to improving the estimation of battery life. In one embodiment, a method includes measuring electrochemical data of a battery cell associated with an electrochemical reaction triggered by a test during a diagnostic cycle. The method also includes determining a feature associated with the degradation of the battery cell from the electrochemical data. The method also includes predicting an end-of-life (EOL) of the battery cell by using the feature in a machine learning (ML) model.

Abstract: Therapeutic and diagnostic methods are provided, which methods relate to the induction of expression of calreticulin on phagocytic cells. Specifically, the methods relate to macrophage-mediated programmed cell removal (PrCR), the methods comprising increasing PrCR by contacting a phagocytic cell with a toll-like receptor (TLR) agonist; or down-regulating PrCR by contacting a phagocytic cell with an inhibitor of Bruton's tyrosine kinase (BTK). In some embodiments, an activator of TLR signaling or a BTK agonist is provided in combination with CD4 7 blockade.

Abstract: Aspects of the present invention include methods for determining a transplant category of a subject having a transplant. Common mechanisms of rejection injury are uncovered across different tissue transplants, and provide a means to understand rational drug design. Various sources of tissues are examined form the patient for understanding AR mechanism (graft biopsy), as well as monitoring by minimal invasive means (blood) or non-invasive means (urine for the kidney allograft). For biomarker discovery different categories of markers are examined such as genes, proteins, peptides and antibodies. These biomarkers can help determine the subject's transplant category (e.g., acute allograft rejection (AR), stable allograft (STA), BK viremia, BK nephritis, drug toxicity or chronic allograft injury (CAI), and the like). Also provided are compositions, systems, kits and computer program products that find use in practicing the subject methods. The methods and compositions find use in a variety of applications.

Abstract: Methods to compute gestational age and gestational health and applications thereof are described. Generally, systems utilize analyte measurements to determine a gestational age and gestational health, which can be used as a basis to perform interventions and treat individuals.

Abstract: Human pluripotent stem cells are differentiated in vitro into oligodendro-spheroids comprising oligodendrocytes for use in analysis, screening programs, and the like.

Abstract: The invention provides compositions and methods for high-efficiency genome editing. In some aspects, the invention provides retron-guide RNA cassettes and vectors comprising the cassettes. Also provided are host cells that have been transformed with the vectors. In other aspects, the invention provides retron donor DNA-guide molecules. In some other aspects, methods for genome editing and the screening of genetic loci are provided. In further aspects, methods and compositions are provided for the prevention or treatment of genetic diseases. Kits for genome editing and screening are also provided.

Abstract: Markers of acute myeloid leukemia stem cells (AMLSC) are identified. The markers are differentially expressed in comparison with normal counterpart cells, and are useful as diagnostic and therapeutic targets.

Abstract: A device (“the ExoTIC device”) for the isolation of extracellular vesicles from an extracellular vesicle-containing sample in which the sample is flowed through a membrane in a flow chamber to capture and purify the extracellular vesicles on the membrane. The extracellular vesicles may be washed and collected and utilized in any one of a number of ways including, but not limited to, identifying biomarkers of a disease, identifying the presence of a biomarker in a patient to determine whether a patient has a disease, and therapeutically treating existing diseases by re-introducing the extracellular vesicles, potentially modified, back into a body.

Abstract: Polypeptide compositions comprising a human immunoglobulin constant region with a modified IgG4 hinge region having an N-terminal deletion of 1, 2, 3, 4, 5 amino acids, relative to a native human IgG4 hinge sequence are provided. In some embodiments the hinge sequence further comprises the amino acid substitution S228P (Eu numbering). Polypeptides comprising the modified sequence optionally comprise a binding moiety specific for an epitope that is present on red blood cells; and can provide for reduced agglutination of hematopoietic cells compared to a comparable polypeptide with a native hinge region.

Abstract: A method and device for extracting information from acoustic signals receives acoustic signals by a microphone, processes them in an analog front-end circuit, converts the processed signals from the analog front-end circuit to digital signals by sampling at a rate of less than 1 kHz or more preferably less than 500 kHz; and processes the digital signals by a digital back-end classifier circuit. The analog front-end processing decomposes the received signals into frequency components using a bank of analog N-path bandpass filters having different subband center frequencies.

Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital analysis is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.

Abstract: Methods to compute gestational age and applications thereof are described. Generally, systems utilize analyte measurements derived from a urine sample to determine a gestational age, which can be used as a basis to perform interventions and treat individuals.