Abstract: Described herein are methods and compositions that enable rapid generation of high-order combinations of genetic elements comprising a CRISPR guide sequence and a scaffold sequence, and a barcode for rapid identification of the combination of genetic elements encoded within a single cell or a pooled population. Also described herein compositions of inhibitors of epigenetic genes and methods for reducing cell proliferation and/or treating cancer.

Abstract: A CNC machine shaping tool is efficiently used by monitoring human audible sound during shaping. A sound information set is created for a tool shaping a workpiece. Shaping sounds are recorded and sliced into short term units. A human operator assigns tool condition labels to each slice. Short term units are combined into mid term units. Noise is reduced by profiling. Mid term sound related features of time and frequency domains are extracted. Dimensionality is reduced by robust principal component analysis. The principal component set is balanced, e.g. by SMOTE. A classifier and principal components are selected. An information set of patterns of values of selected principal components for the tool is created. In an industrial setting, shaping sounds are recorded, noise reduced and select principal component vector values are compared to the tool condition labeled patterns of values in the information set to identify tool condition before deterioration.

Abstract: A video camera captures images of retroreflection from the retina of an eye. These images are captured while the eye rotates. Thus, different images are captured in different rotational positions of the eye. A computer calculates, for each image, the eye's direction of gaze. In turn, the direction of gaze is used to calculate the precise location of a small region of the retina at which the retroflection occurs. A computer calculates a digital image of a portion of the retina by summing data from multiple retroreflection images. The digital image of the retina may be used for many practical applications, including medical diagnosis and biometric identification. In some scenarios, the video camera captures detailed images of the retina of a subject, while the subject is so far away that the rest of the subject's face is below the diffraction limit of the camera.

Abstract: An assembly that includes a printed circuit board and a foam dielectric material, and a method of fabricating the assembly is disclosed. The assembly includes at least one layer of a foam dielectric material, which has properties similar to those of air. This layer of foam dielectric material is disposed between a top sublaminate and a bottom sublaminate. The bottom sublaminate may be a traditional printed circuit board, comprising an arbitrary number of layers. The top sublaminate may be a single layer, or may be multiple layers and may include an antenna. The foam dielectric material serves to provide mechanical support for the top sublaminate and the central conductor. The foam dielectric material also provides physical separation between the bottom sublaminate and the antenna.

Abstract: A method for preparing a core-shell nanocrystal can include mixing an M-containing precursor solution, an X-containing precursor solution, and an acid or alcohol in an inert atmosphere at a first temperature to form a reaction mixture; maintaining the reaction mixture at the first temperature to grow the MX core of the nanocrystal; raising the temperature of the reaction mixture to a second temperature; and maintaining the reaction mixture at the second temperature to grow a shell of the nanocrystal.

Abstract: Conventional methods for imaging transient targets are constrained by a trade-off between resolution and frame rate, and transient targets moving faster than the detector frame typically result in image blurring. Imagers using digital-pixel focal plane arrays (“DFPAs”) have on-chip global pixel operation capability for extracting a single transient-feature (i.e., single-frequency discrimination) in a snapshot that depends on the number of counters implemented per pixel. However, these DFPA systems are not capable of multi-target and multi-frequency discrimination. Imagers described herein achieve multi-target transient signature discrimination orders of magnitude faster than the readout frame rate using in-pixel electronic shuttering with a known time-encoded modulation. Three-dimensional (x,y,t) data cube reconstruction is performed using compressive sensing algorithms.

Abstract: The present invention provides tools and methods for the systematic analysis of genetic interactions between cells. The present invention provides tools and methods for modulating cell phenotypes and compositions, combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.

Abstract: A microfluidic system for causing perturbations in a cell membrane, the system including a microfluidic channel defining a lumen and being configured such that a cell suspended in a buffer can pass therethrough, wherein the microfluidic channel includes a cell-deforming constriction, wherein a diameter of the constriction is a function of the diameter of the cell.

Abstract: A method for precise control of movement of a motive phase on a lubricant-impregnated surface includes providing a lubricant-impregnated surface, introducing the motive phase onto the lubricant-impregnated surface, and exposing the droplets to an electric and/or magnetic field to induce controlled movement of the droplets on the surface. The lubricant-impregnated surface includes a matrix of solid features spaced sufficiently close to stably contain the impregnating lubricant therebetween or therewithin. The motive phase is immiscible or scarcely miscible with the impregnating lubricant.

Abstract: A cryogenic electronic package includes at least two superconducting and/or conventional metal semiconductor structures. Each of the semiconductor structures includes a substrate and a superconducting trace. Additionally, each of the semiconductor structures includes a passivation layer and one or more under bump metal (UBM) structures. The cryogenic electronic package also includes one or more superconducting and/or conventional metal interconnect structures disposed between selected ones of the at least two superconducting semiconductor structures. The interconnect structures are electrically coupled to respective ones of the UBM structures of the semiconductor structures to form one or more electrical connections between the semiconductor structures. A method of fabricating a cryogenic electronic package is also provided.

Abstract: Quality control of a periodic structure is performed using the damping rate of acoustic waves generated in the periodic structure. In this technique, an excitation light beam illuminates the first layer in the periodic structure to excite an acoustic wave. Possible irregularities in the periodic structure can scatter the acoustic wave, thereby increasing the damping rate of the acoustic wave. A sequence of probe light beams illuminates the periodic structure to measure the acoustic wave as a function of time to generated a temporal signal representing the damping rate of the acoustic signal. The acquired damping rate is employed to evaluate the quality of the periodic structure.

Abstract: An imaging system includes a metering structure and a plurality of foldable members disposed around a periphery of the metering structure. Each foldable member in the plurality of foldable members includes an arm comprising a strain deployable composite and a reflector disposed on the arm. The arm in a respective foldable member in the plurality of foldable members is configured to hold the respective foldable member toward the metering structure in a first state and to hold the respective foldable member away from the metering structure in a second state such that the reflector of the respective foldable member forms part of a sparse aperture in the second state.

Abstract: An apparatus for generating a spectral image includes a filter to receive incident light. The filter has a variable refractive index. The apparatus also includes a modulator, operably coupled to the filter, to modulate the variable refractive index of the filter so as to generate a plurality of optical patterns from the incident light. The plurality of optical patterns represents the spectral image and each optical pattern in the plurality of optical patterns corresponds to a different modulation of the variable refractive index. The apparatus further includes a detector, in optical communication with the filter, to detect the plurality of optical patterns.

Abstract: Geometries of the structures and objects deviate from their idealized models, while not always visible to the naked eye. Embodiments of the present invention reveal and visualize such subtle geometric deviations, which can contain useful, surprising information. In an embodiment of the present invention, a method can include fitting a model of a geometry to an input image, matting a region of the input image according to the model based on a sampling function, generating a deviation function based on the matted region, extrapolating the deviation function to an image wide warping field, and generating an output image by warping the input image according to the warping. In an embodiment of the present invention, Deviation Magnification inputs takes a still image or frame, fits parametric models to objects of interest, and generates an output image exaggerating departures from ideal geometries.

Abstract: Provided is a method for correcting errors in a data transmission network, comprising: transmitting a plurality of uncoded information packets across a network path; transmitting a plurality of coded packets for recovering information packets lost in transmission across said network path, the coded packets being temporally interspersed among said uncoded information packets, wherein the coded packets are encoded based on information packets transmitted prior to a previously transmitted coded packet; and determining the interspersion of the coded packets according to a packet loss rate.

Abstract: Described herein are methods of preventing or reducing fibrosis comprising administering CSF1R inhibitors, coating formulations comprising CSF1R inhibitors, coatings comprising CSF1R inhibitors for implantable medical devices, CSF1R inhibitor coated implantable medical devices, as well as corresponding embodiments comprising additional agents.

Abstract: A disordered rocksalt lithium metal oxide and oxyfluoride as in manganese-vanadium oxides and oxyfluorides well suited for use in high capacity lithium-ion battery electrodes such as those found in lithium-ion rechargeable batteries. A lithium metal oxide or oxyfluoride example is one having a general formula: LixM?aM?bO2-yFy, with the lithium metal oxide or oxyfluoride having a cation-disordered rocksalt structure of one of (a) or (b), with (a) 1.09?x?1.35, 0.1?a?0.7, 0.1?b?0.7, and 0?y<0.7; M? is a low valent transition metal and M? is a high-valent transition metal; and (b) 1.1?x?1.33, 0.1?a?0.41, 0.39?b?0.67, and 0?y?0.3; M? is Mn; and M? is V or Mo. The oxides or oxyfluorides balance accessible Li capacity and transition metal capacity. An immediate application example is for high energy density Li-cathode battery materials, where the cathode energy is a key limiting factor to overall performance. The second structure (b) is optimized for maximal accessible Li capacity.

Abstract: A sensor can include a redox-active complex. The sensor can be voltage sensitive.

Abstract: Methods and systems for the fabrication of composite materials are generally described. Certain inventive methods and systems can be used to fabricate composite materials with few or no defects. According to certain embodiments, composite materials are fabricated without the use of an autoclave. In some embodiments, composite materials are fabricated in low pressure environments.

Abstract: The invention relates to methods and compositions for promoting cognitive function and/or treating cognitive function disorders and impairments. In particular the methods are accomplished by administering to a subject a specific CDK5 peptide inhibitor and a pharmaceutically acceptable carrier.