Abstract: This invention provides methods for treating diseases associated with elevated p38 mitogen-activated protein kinase activity. Moreover, the invention provides methods for testing a candidate compound for a p38 mitogen-activated protein kinase modifying activity by calculating the level of relocalization of an SMN complex component from the cytoplasm to the nucleus of a cell. Additionally, the invention provides a kit and a system for calculating the same.

Abstract: In one example, a bearing or transmission has an outer race that extends circumferentially about a central axis. An inner race is disposed within the outer race and extends circumferentially about the central axis so as to be coaxial with the outer race. A plurality of load-bearing rolling elements are disposed between the inner race and the outer race, and are spaced circumferentially from one another so as to not contact one another. A plurality of separator rolling elements are disposed within the outer race. Each separator rolling element is disposed between a respective pair of the load-bearing rolling elements such that the separator rolling element is in rolling contact with the load-bearing rolling elements of the respective pair. The load-bearing and separator rolling elements form a contiguous ring.

Abstract: Provided are methods for forming polymer-infiltrated nanoparticle films by using capillary action to draw mobile molecular chains into the pores of a bed of nanoparticles. The chains can spread across the entire bed of nanoparticles. The disclosed methods also provide the formation of patterned polymer-infiltrated nanoparticle film compositions, as well as laterally graded compositions and compositions that feature a polymer gradient through the composition's thickness. Articles can be formed that include a plurality of polymer types infiltrated into the bed of nanoparticles.

Abstract: Multi-state switchability is highly desirable in optoelectronic devices. For liquid crystal (LC) based devices, the stability of any configuration is achieved through a balance between imposed interactions and the LC's orientational elasticity. In most cases, the latter acts to resist deformation. By combining surface topography and chemical patterning, provided here are the effects of saddle-splay orientational elasticity, a property that, despite being intrinsic to all LCs, is routinely suppressed. Utilizing theory and continuum elastic calculations, provided here are example conditions for which, even using generic, achiral LC materials, spontaneously broken surface symmetries develop. Also provided are multi-stable devices in which a weak, but directional, applied field switches between spontaneously-polar surface state domains. The disclosed approach is useful in low-field and fast-switching optoelectronic devices, beyond those attainable by current technologies.

Abstract: This disclosure provides a culture media that facilitates survival and/or engraftment of transplanted cells. In one embodiment, this culture media comprises collagen I, albumin, L-glutamine and NaHCO3. In one embodiment, this culture media promotes enhanced survival and engraftment of ?-cells of human pancreatic islets transplanted under the skin. The protective effect of this culture media is mediated, at least partly, by upregulating anti-apoptotic signaling pathways.

Abstract: The disclosure relates to immune cells comprising one or more vectors comprising a nucleic acid sequence encoding a chimeric antigen receptor specific for CD19 and a nucleic acid sequence encoding an enhancer of T cell priming (e.g., IL-18), compositions comprising the T cells, and methods of generating and/or using the T cells to treat diseases associated with the expression of CD19.

Abstract: The present disclosure provides compositions and methods for site-specific labeling of antibodies by proximity-based sortase-mediated ligation. The ligation method utilizes a non-canonical isopeptide ligation reaction catalyzed by newly identified variants of S. aureus sortase A. An antibody binding domain (e.g., protein A or protein G) is fused to the variants of SrtA to bring the enzyme into close proximity of an antibody, thereby significantly increases the efficiency of isopeptide bond formation.

Abstract: The invention provides compositions and methods for treating asthma in a subject in need thereof comprising administering chemically synthesized secoisolaricirecinol diglucoside (SDG), and in particular, a racemic mixture of the SDG (LGM2605), stereoisomers thereof, metabolites thereof, and analogs thereof. Also provided are methods for treating or preventing ozone-induced damage in a subject in need thereof.

Abstract: The present invention includes compositions and methods for modifying primary T cells. In one aspect, the invention comprises administering to a cell a stem-loop Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) RNA (st-crRNA) and a Cpf1 enzyme.

Abstract: Methods and apparatus for improving cognitive function within a human. The invention utilizes a neurostimulation device, such as a signal generator, to affect tissue elements at a lateral temporal lobe of the human brain. The implanted device delivers treatment therapy to thereby improve cognitive function by the human. A sensor may be used to detect various characteristics of cognition. A microprocessor algorithm may then analyze the output from the sensor to regulate delivery of the stimulation therapy.

Abstract: Compounds comprising peptides capable of binding C3 protein and inhibiting complement activation are disclosed. The compounds comprise compstatin analogs in which the N-terminus and/or C-terminus contains an added component that improves (1) the peptide's solubility at physiological pH; (2) the peptide's plasma half-life; (3) the peptide's intraocular retention; and/or (4) the peptide's binding affinity to C3 or its fragments as compared to an unmodified compstatin peptide under equivalent conditions. Pharmaceutical compositions and methods of using the compounds are also disclosed.

Abstract: Compositions and methods are provided for treating ocular neuropathy in a subject. In one aspect, a recombinant adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding NRF2. In another aspect, a recombinant adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding SIRT1. In desired embodiments, the subject is human, cat, dog, sheep, or non-human primate.

Abstract: The invention provides a polynucleotide sequence (e.g., a gene, e.g., DNA or RNA) encoding UGT1A1 (e.g., expressing human UGT1A1). The invention further provides a vector, such as an adeno-associated virus (AAV) vector (e.g., AAV8) having a vector genome including inverted terminal repeat sequences and a UGT1A1 coding sequence operably linked to one or more expression control sequences (e.g., expression control sequences including a liver-specific promoter). Also provided are compositions containing these AAV vectors and methods of treating Crigler-Nijjar syndrome type I, Crigler-Nijjar syndrome type II, and Gilbert syndrome.

Abstract: Immunogenic compositions and methods of their use in eliciting immune responses to coronaviruses, such as SARS-CoV-2 are provided.

Abstract: Provided herein are compositions including brain-capillary binding and/or blood-brain barrier crossing (BBB) tissue-targeting peptides linked thereto or inserted in a targeting protein of a recombinant vector having at least one exogenous peptide comprising an amino acid sequence of Y-G/A/R/K-Y/H-GNPA-T/R/H-RYFD-V/K. Compositions providing such conjugates, targeting peptides, or recombinant vectors having a mutant capsid or envelope protein are provided as are uses thereof.

Abstract: The present invention provides compositions and methods for generating and cryopreserving dendritic cells with superior functionality in producing stronger signals to T cells, resulting in a more potent DC-based anti-tumor vaccine. The present invention includes mature, antigen loaded DCs activated by Toll-like receptor agonists that induce clinically effective immune responses, preferably when used earlier in the disease process. The DCs of the present invention produce desirable levels of cytokines and chemokines, and further have the capacity to induce apoptosis of tumor cells. The cells can be cryopreserved and thawed for later use, thereby reducing the need for repeated pheresis and elutriation processes during vaccine production. These methods can also be utilized to directly target molecules involved in carcinogenetic signaling pathways and cancer stem cells.

Abstract: The subject matter described herein relates to laminated magnetic cores, methods of fabricating laminated magnetic cores, and electric devices using laminated magnetic cores. In some examples, a method for fabricating a laminated magnetic core includes depositing a first magnetic layer and depositing an interlamination layer of over the first magnetic layer. The interlamination layer comprises a partially conducting material having a conductivity greater than or equal to 10?4 S/cm and less than or equal to 105 S/cm. The method includes depositing a second magnetic layer over the interlamination layer. The method can include sequentially depositing additional interlamination layers and additional magnetic layers in an alternating fashion to produce the laminated magnetic core.

Abstract: This present disclosure relates to a bioengineering approach based on microphysiological culture to mimic tissue-tissue interface. Accordingly, the present disclosure provides methods, compositions and kits related to the approach.

Abstract: Methods, systems, and computer readable media for using metadata tag compression. A method occurs at a metadata processing system for enforcing security policies in a processor architecture. The method comprises: receiving, at the metadata processing system, a short tag associated with a word in memory; translating the short tag, using a tag map, into a long tag, wherein the short tag indicates a location of the long tag relative to an offset in the tag map and wherein the long tag indicates a memory location containing metadata associated with the word or an instruction; obtaining the metadata from the memory location; and determining, using the metadata, whether the word or the instruction violates a security policy.

Abstract: A dual vector system for treating a genetic disorder is provided. The system includes (a) a gene editing vector comprising an expression cassette comprising a nucleic acid sequence encoding a nuclease and regulatory sequences that direct expression of the nuclease in a target cell comprising a PCSK9 gene; and (b) a donor vector comprising a nucleic acid sequence encoding an exogenous product for expression from the PCSK9 locus, wherein the inserted nucleic acid sequence does not encode PCSK9, wherein the system further comprises sequences that direct the nuclease to specifically targets the native PCSK9 gene locus; and wherein the native PCSK9 in the target cell is optionally ablated or reduced post-dosing with the dual vector system.