Abstract: Endogenous and exogenous gene directed expression of protein synthesis in Escherichia coli is enhanced by transformation to incorporate a dominant mucoid mutant allele of the capR (lon) gene, preferably through use of the DNA plasmid, pBZ201M9 (A.T.C.C. Plasmid No. 40041) which carries the capR9 mutant allele.

Abstract: This disclosure is directed to an isoelectric focusing apparatus, wherein stabilization of the fluid containing the isolated proteins is achieved by carrying out the separation in a rotating cylinder with the separation cavity of the cylinder being segmented by means of filter elements. The filter elements are constituted of a material offering some degree of resistance to fluid convection, but allowing relatively free and unhindered passage of current and transport of proteins. The combined effect of segmentation and rotation has been found to be superior to either segmentation or rotation alone in maintaining the stability of the migrated fractions.

Abstract: Microencapsulation of solid phase scintillators in gels selectively permeable to diffusible radioactive label. These encapsulated scintillators are used to monitor the concentration of radioactive-tagged subtances in fluid systems.

Abstract: Photographic and Photolithographic systems are provided which utilize diacetylenic materials as components thereof. Such materials, which include at least two acetylenic bonds in conjugation with one another, provide a combination of both high sensitivity and high resolving power, thus resulting in superior photographic and photolithographic properties.In accordance with a preferred embodiment of the invention, a substrate is coated with at least one layer of a polymerizable composition comprising a diacetylenic species, said layer being organized into a plurality of domains having a substantially regular array of the diacetylenic species. In other embodiments, covalent bonding of the diacetylenic layer to the substrate is accomplished through the use of suitably constituted silane species, thus to provide a highly beneficial adhesion of substrate and photo- or photolithographic layer.

Abstract: A class of dipeptide derivatives having opiate activity having the formulaTyrosyl--X--Ywherein X is a D- or L-amino acid radical and Y is an amide or substituted amide are disclosed.

Abstract: The transcription of DNA sequences in living cells is subjected to external regulation by incorporation of promoter/regulator DNA sequences responsive to metals and/or steroids. More particularly, regulation of the transcription of selected exogenous DNA sequences incorporated into eukaryotic host cells is facilitated by operative association (e.g., fusion) of the selected sequence to a promoter/regulator DNA sequence which is positively or negatively responsive to environmental variation in the concentration of heavy metal ions and/or steroid hormones. As an example, a structural gene for thymidine kinase from herpes simplex virus, fused to the promoter/regulator DNA sequence of a mouse metallothionein-I gene and incorporated on a suitable DNA plasmid vector, is introduced into mouse embryos and stably incorporated therein.

Abstract: The compounds of the present invention are irreversible and highly selective inhibitors of the cytochrome P-450 cholesterol side chain cleavage (P-450scc) enzyme responsible for the first and rate limiting step of steroid hormone biosynthesis by the adrenal glands.

Abstract: The present invention relates to a new class of novel Rhodamine derivatives. These derivatives which are bisamide substitution products are nonfluorescent. Whereas the monoamide substitution products exhibit high fluorescence. Cleavage of a single specified amide bond by bond-specific proteinases will therefore convert the nonfluorescent bisamide derivative into a highly fluorescent monoamide derivative.

Abstract: Hybridoma tumor cell line (ATCC Number CRL 8164). A monoclonal anti-erythropoietin antibody substance, Epoclonalan, produced by said cell line. Use of Epoclonalan in immonological methods for isolation of natural erythropoietin and for quantitative detection of erythropoietin in fluid samples.

Abstract: Ferroelectric (chiral) smectic liquid crystal compounds having an achiral core and chiral tail units derived from lactic acid possess the desired combination of properties temperature range, ferroelectric polarization, and low orientational viscosity. These newly made compounds exhibit high speed, multistate electro-optic switching properties which make them ideally suited to certain electro-optic and display device applications.

Abstract: A non-invasive birth control device in the form of a custom-formed, valved cervical cap is disclosed. The cervical cap comprises a cup-shaped elastomeric shell substantially complementary with contiguous surface of portio vaginalis cervicis when in contact therewith and having an aperture at the apex of the shell. The aperture is covered by an elastomeric web secured to the shell and defines, together with the shell, a one-way valve means having a discharge port offset from the aperture. The cervical cap can be fabricated from a specially designed blank of a thermoplastic elastomeric material using a replica of cervix uteri as a mold.

Abstract: Novel nonlinear optical, piezoelectric, pyroelectric, waveguide, and other materials are presented together with processes for their employment and articles formed thereby. Such materials, processes and articles comprise diacetylenes and polymers formed from diacetylenic species, which polymers are amenable to close geometric, steric, structural, and electronic control. Accordingly, it is now possible to design, formulate and employ new diacetylenic species and polymers formed therefrom to provide nonlinear optic, waveguide, piezoelectric, and pyroelectric materials and devices having surprising efficiencies and degrees of effect.According to a preferred embodiment of the invention, diacetylenes which are crystallizable into crystals having a non-centrosymmetric unit cell may form single crystals or may be elaborated into a thin film upon a substrate by the Langmuir-Blodgett technique. Such films may, optionally, be polymerized either thermally or by irradiation for use in nonlinear optical and other systems.

Abstract: Method and apparatus for detecting the fertile and infertile phases of the human female menstrual cycle based upon observation of cyclic volumetric and gravimetric changes in cervico-vaginal fluid.

Abstract: A conforming valved cervical cap assembly is provided with an elastomeric inner layer molded and cured in situ adjacent the exocervical surface of a cervix uteri. The inner layer is wettable, non-porous and matingly fits and resiliently complements the exocervical surface. In order to prevent the molding material from clogging or otherwise impairing operation of the valve, a valve cover is detachably connected to the valve during molding and curing.

Abstract: Cariogenic substances such as foodstuffs are treated to incorporate a soluble source of calcium or phosphate ions to provide a product of calcium and phosphate ion concentrations in fluids of salivary retention areas preclusive of carious dissolution of dental enamel. In non-aqueous foodstuffs, the calcium or phosphate ion source should be at least as rapidly soluble as cariogenic, e.g., carbohydrate, components therein. Also provided are mouth rinse solutions comprising a soluble source of calcium or phosphate ions and an osmotically active agent effective in delivering such ions to fluids trapped in salivary retention areas.

Abstract: The present invention discloses a novel and unique family of agents which reversibly binds molecular oxygen at room temperature.

Abstract: Compounds of the formula: ##STR1## wherein R.sub.1 is a moiety selected from the group consisting of ##STR2## R.sub.2 -R.sub.22 are amino acid moieties wherein R.sub.2 is an optional moiety which when present is selected from the group consisting of Ser and Gly,R.sub.8 is Leu, Val, or Ile,R.sub.10 is Gln, Lys, or Gly,R.sub.11, R.sub.14, and R.sub.20 are each independently selected from the group consisting of Gln and Lys,R.sub.12 is Leu or Trp,R.sub.13 is Gln or SerR.sub.17 is Gln or His,R.sub.21 is Gln or Thr,R.sub.

Abstract: Hybridoma tumor cell line A.T.C.C. No. HB8116. An anti-H-Y antigen monoclonal antibody substance, "Hyclonalan," produced by said cell line. Use of Hyclonalan in immunoselection methodology.

Abstract: A contraceptive agent is provided which comprises biologically-active alpha-lactalbumin. The agent can be administered to female mammals using a variety of pharmaceutically acceptable vehicles, provided the alpha-lactalbumin retains its biological activity in the presence of the vehicle.

Abstract: A method for the cutting of holes 20 Angstroms in diameter, or lines 20 Angstroms wide in a material having positive ionic conduction by the use of a focused electron probe is described. The holes and lines are stable under ambient conditions.