Abstract: The invention relates to methods and media for preparing and maintaining self-renewing pluripotent embryonic stem cells. The methods include, in some embodiments, culturing embryonic stem cells in culture medium that includes culture medium conditioned by cells that express wnt3a.

Abstract: A launch system and method for microscopy having an optical fiber positioned proximate a sample slide with an optical fiber mounting element so as to deliver an EMR from a terminal end of the optical fiber across a surface of the sample slide to an opposing side surface at a desired incident angle.

Abstract: The invention involves methods of regulating cell growth and division to control disease processes by manipulating mitochondrial metabolism and the expression of cell surface immune proteins. The invention also involves related compositions and screening assays.

Abstract: The invention involves methods of regulating cell growth and division to control disease processes by manipulating mitochondrial metabolism and the expression of cell surface immune proteins. The invention also involves related compositions and screening assays.

Abstract: We have now discovered that eukaryotes, including mammals, have a DNA mismatch repair pathway analogous to the pathway that exists in bacteria. Defects or alterations in this mismatch repair pathway in a mammal, such as a human, will result in the accumulation of unstable repeated DNA sequences. Such a phenotype has a high correlation to disease state in a number of cancers, such as hereditary colon cancers. Accordingly, discovering a defect or alteration in the pathway can be diagnostic of a predisposition to cancer, and prognostic for a particular cancer. We have also discovered and sequenced one of the genes in this pathway in a number of mammals, including humans. This gene, referred to herein as MSH2, has many applications. It can be used in assays, to express gene product, for drug screens, and therapeutically. We also disclose herein a method for screening for other genes in this mismatch repair pathway.

Abstract: An in-situ monitoring system is engaged with a mechanical system for which it is desired to assess or monitor the in-situ performance of the mechanical system. The in-situ monitoring system includes at least one input sensor and at least one output sensor for collecting data corresponding, respectively, to input into, and output from, the mechanical system. The monitoring system also includes an analyzer that utilizes the input and output data to generate an in-situ response representation of the mechanical system. The in-situ representation may be used to make a decision regarding the status of the mechanical system, e.g., to replace all or part of it, to adjust it or to do nothing. In one embodiment, the analyzer is programmed to adapt to the operating conditions of the mechanical system by automatically selecting which one of a number of target representations should be used to characterize the in-situ representation.

Abstract: The invention involves methods of regulating cell growth and division to control disease processes by manipulating mitochondrial metabolism and the expression of cell surface immune proteins. The invention also involves related compositions and screening assays.

Abstract: A femoral component is provided for use in a knee joint prosthesis. The femoral component may be configured to provide one or more desirable kinematic relationships with a tibial component and/or patella so as to mimic the flexion and extension motion of a natural knee joint. The femoral component may be configured so that the patella follows a substantially circular pathway during knee flexion and extension. The femoral component may be configured so that the patella follows a curved patellar path during flexion and extension, and the curved patellar path has an origin located anterior and proximal to the geometrical center axis of the knee. The femoral component may be configured so that the patella follows a curved patellar path that lies in a plane which is parallel to and offset from a plane that extends through the center of the femoral head of a femur and is perpendicular to the geometric center axis of the knee.

Abstract: Systems and methods that utilize semiconductor molecules to form crystalline thin-films by depositing the molecules into a substrate at a lateral growth front. Techniques embodied in corresponding ones of the disclosed systems and methods include a submersion technique in which a substrate is submerged in a precursor solution containing the molecules and a film is grown at a meniscus formed between the free surface of the solution and the substrate. Another disclosed technique is a mask technique in which a film is grown on a substrate through an aperture of a moving mask be exposing the aperture to the molecules. Yet another technique disclosed is a writing technique in which a pen is used to deliver to a substrate a precursor solution containing the molecules and the film is grown as the solvent evaporates from the delivered solution.

Abstract: The invention involves methods of regulating cell growth and division to control disease processes by manipulating mitochondrial metabolism and the expression of cell surface immune proteins. The invention also involves related compositions and screening assays.

Abstract: A modularized phosphorous filter (P-filter) for installing into open flow channels, such as urban and rural drainage ditches, streams, rivers, bayous, swales, etc. In some embodiments, the P-filter includes a module formed from a container that contains a phosphorous-filtering mass of loose pieces of slag. The container has anchoring extensions for anchoring the P-filter into the sides of the channel into which it is installed. In other embodiments, the P-filter is made of a number of P-filter modules each including a container that contains a phosphorous-filtering mass of loose pieces of slag. The P-filter modules are stacked with, and/or otherwise arranged relative to, one another so as to form a modularized P-filter. Preferably, the P-filter modules are sized to be readily movable by one or more people and/or power equipment, such as a tractor having a backhoe.

Abstract: Systems for and methods of assessing urinary flow rate via sound analysis. Embodiments of the uroflow measurement systems include a computer and a telephone or a digital recording mechanism to capture the sound of one or more urination events, which are stored as audio files in a database The uroflow measurement systems may include sound analysis software for analyzing the strength and duration of each urination event and may include a web-based uroflow software application for viewing the results via the Internet or other network In one embodiment, the sound analysis software performs the steps of reading in the raw data of a selected audio file, generating a plot of the audio signal amplitude vs.

Abstract: A crystalline thin structure (104, 204, 404) is grown on a surface (108, 228) of a substrate (112, 208, 400) by depositing molecules (136, 220) from a molecular precursor to a lateral growth front (144, 224) of the structure using a crystal grower (116, 200). In one embodiment, the crystal grower comprises a solution (124) containing the molecular precursor in a solvent (140). Molecules are added to the lateral growth front by moving one or both of the free surface (120, 120?) of the solution and deposition surface relative to the other at a predetermined rate. In another embodiment, the crystal grower comprises a mask (212) that includes at least one opening (216). Precursor molecules are vacuum deposited via a molecular beam (236) at the growth front (228) of the crystalline thin structure (204) as one or both of the opening and surface are moved relative to the other at a predetermined rate.

Abstract: A system for exposing a target material to small particles. The system includes an exposure chamber that receives the target material. A stream of charged particles is directed via an inlet into the exposure chamber toward the target material. One or more electrodes are located relative to the target material and the inlet, and are electrically charged, so as to cause at least some of the charged particles to impact upon the target material. The system can be used to expose the target material to small, for example, nanoscale, particles in a gas environment.

Abstract: The present invention, in some aspects, relates to systems and methods for determining oxidized proteins, including glutathionylated proteins such as S-glutathionylated proteins. The systems and methods of the invention can be used in vitro (e.g., in cell or tissue culture) or in vivo, for example, to diagnose a person having an oxidative stress condition. For instance, in some cases, the invention can be used to spatially determine the location and/or concentration of oxidized proteins within cells and/or tissues (e.g., through visual detection). In one set of embodiments, a glutathionylated or otherwise oxidized moiety on a protein may be reacted with a detection entity, which may be, for example, fluorescent, radioactive, electron-dense, able to bind to a signaling entity or a binding partner in order to produce a signal, etc.

Abstract: The invention relates to compositions of mesoporous inorganic oxide particles and methods of use. The particles may be coated with a polyethylene glycol group. The compositions of the invention are useful for delivering therapeutic and diagnostic materials to an organism.

Abstract: Self-healing cable apparatus and methods are disclosed. The cable has a central core surrounded by an adaptive cover that can extend over the entire length of the cable or just one or more portions of the cable. The adaptive cover includes a protective layer having an initial damage resistance, and a reactive layer. When the cable is subjected to a localized damaging force, the reactive layer responds by creating a corresponding localized self-healed region. The self-healed region provides the cable with enhanced damage resistance as compared to the cable's initial damage resistance. Embodiments of the invention utilize conventional epoxies or foaming materials in the reactive layer that are released to form the self-healed region when the damaging force reaches the reactive layer.

Abstract: The invention relates to products and methods for determining, localizing, and quantitating single-stranded polynucleotides. The methods of the invention include the use of Gp32F protein and mutant Gp32F protein to monitor polynucleotide reactions such as DNA replication, annealing, and excision and to determine single-stranded polynucleotide structures such as gaps, flaps, and bubbles.

Abstract: We have now discovered that eukaryotes, including mammals, have a DNA mismatch repair pathway analogous to the pathway that exists in bacteria. Defects or alterations in this mismatch repair pathway in a mammal, such as a human, will result in the accumulation of unstable repeated DNA sequences. Such a phenotype has a high correlation to disease state in a number of cancers, such as hereditary colon cancers. Accordingly, discovering a defect or alteration in the pathway can be diagnostic of a predisposition to cancer, and prognostic for a particular cancer.

Abstract: The invention provides methods and compositions for treating subjects using Grb7 antagonists. Specifically disclosed are Grb7 antagonists that bind selectively to Grb7 and interfere with the ability of Grb7 to bind to its native ligands. These compositions are useful in the prevention and treatment of disorders characterized by abnormal interaction of Grb7 with its native ligands (e.g., ErbB2).