Abstract: The disclosure addresses methods, compositions, and kits used to detect or quantify polymerase inhibitors in biological samples. The polymerase inhibitors can be therapeutic agents, or metabolites thereof, that have been administered to a subject as part of, for example, antiretroviral therapy (ART) or pre-exposure prophylaxis (PrEP) to address potential infections by, e.g., retroviruses such as HIV and other viruses reliant on reverse transcription. These methods, compositions, and kits can be applied to monitor a subject's compliance with the indicated therapies and can inform potential adjustments to the therapies.

Abstract: A carbon nanotube-based thin-film resistive sensor is disclosed. The sensor includes carbon nanotube film functionalized with sensing moieties and is configured for use in rapid screening for pathogens in point of care settings.

Abstract: Methods of fabricating 3D printed structures from biocompatible proteins include forming a photoreactive, proteinaceous resin, and 3D printing biocompatible structures from the resin by the patterned application of light in a select wavelength to cure the resin into the desired structures. Suitable photoreactive proteinaceous resins can be formed by reacting an aqueous solution of an acrylated or methacrylated globular protein with a photoreactive comonomer or photoinitiator. Structures printed from the photoreactive, proteinaceous resin can be photo-cured and dried to form bioplastic structures.

Abstract: Fluid access devices include a machine-side hydraulic circuit and a patient-side hydraulic circuit, and are configurable between a connected state and at least one disconnected state. In the connected state, fluid flows between the machine-side hydraulic circuit and the patient-side hydraulic circuit. In the disconnected state, fluid does not flow between the machine-side hydraulic circuit and the patient-side hydraulic circuit. In some disconnected states, fluid recirculates through at least one of the machine-side hydraulic circuit or the patient-side hydraulic circuit in the disconnected state.

Abstract: In some embodiments, an augmented reality system is provided. The augmented reality system is configured to detect real-world objects, create software objects that represent the real-world objects, receive requests from applications to present virtual objects at locations associated with the real-world objects, and to present the virtual objects. In some embodiments, an operating system of the augmented reality system is configured to resolve conflicts between requests from multiple applications. In some embodiments, the operating system of the augmented reality system is configured to provide information to applications to allow the applications to avoid or resolve conflicts amongst themselves.

Abstract: Described herein are compositions and methods for treating Alzheimer's disease or dementia. The compositions include mammalian suppressor of taupathy 2 inhibitors (MSUT2). The MSUT2 inhibitors can be small interfering RNAs, guide RNAs, or small molecules. The methods include reducing accumulation of phosphorylated and aggregated human tau.

Abstract: Zwitterionic carboxybetaine copolymers and their use in coatings to impart non-fouling and functionality to surfaces, particularly surfaces of blood-contacting medical devices.

Abstract: The disclosure provides compositions and methods for delivering a payload to cells or tissues that express GLUT4. In some embodiments, the compositions comprise an antibody, or fragment or derivative thereof, that specifically binds to glucose transporter 4 (“GLUT4”) protein, and a therapeutic payload conjugated thereto. In some exemplary embodiments, the compositions are useful for methods of treating a disease or condition in a subject with a genetic mutation in a gene encoding dystrophin protein, wherein the payload comprises a nucleic acid encoding a functional dystrophin protein or functional fragment thereof to ameliorate aspects of the disease.

Abstract: There is provided a mechanical avatar assembly for use in a confined space in a structure. The mechanical avatar assembly includes a rail assembly for attachment to an access opening to the confined space. The rail assembly includes two or more rail segments coupled together to form an elongated base having a rail and a gear rack extending along a length of the elongated base. The rail assembly further includes a carriage portion coupled to the rail, and movable relative to the rail, and a drive assembly coupled to the carriage portion and to the gear rack, to move the carriage portion along the rail. The mechanical avatar assembly further includes an articulating avatar arm coupled to, and movable via, the carriage portion. The mechanical avatar assembly further includes an image capturing device.

Abstract: The behavior of automated agents, such as autonomous vehicles, drones, and the like, can be improved by control systems and methods that implement a combination of neighbor following behavior, or neighbor-averaged information transfer, with delayed self-reinforcement by utilizing time-delayed movement data to modify course corrections of each automated agent. Disclosed herein are systems and methods by which a follower agent, or a multiple follower agents in formation with a plurality of automated agents, can be controlled by generating course correction data for each follower agent based on the movement of neighboring agents in formation, and augmenting the course correction data based on time-delayed movement data of the follower agent.

Abstract: The disclosure provides compositions, systems, and related method for using nanopore-based detection of nucleic acid displacement circuits. In some embodiments, output strands contain orthogonal barcode sequences that are captured by nanopore systems to produce a unique and recognizable current signal. Machine learning models can be used to differentiate a plurality of current signals and, therefore, monitor detection and quantification of multiple nucleic acid displacement circuits in a single pot reaction.

Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

Abstract: An antimicrobial preventive netting is disclosed that includes one or more photosensitizers that are capable of generating a cloud of singlet oxygen on each surface and openings through the netting in response to incident light. A depth of the singlet oxygen cloud may be less than 0.7 centimeters from a netting surface. The netting may be supported by screens, enclosures or headgear. The netting can be made by dipping or soaking the netting in a solution of one or more photosensitizers.

Abstract: Disclosed herein are devices, systems, and methods that provide improved tunneling magnetoresistance (TMR) through the use of innovative device structures and heterostructure layers therein. Particularly, two or more magnetic layers form a heterostructure core of the switching device, with control of current passing through the heterostructure determined by an applied magnetic field that modifies the magnetization of the heterostructure from a ground magnetic state that is layered antiferromagnetic.

Abstract: Methods of labeling or barcoding molecules within one or more portions of a plurality of cells are provided. Kits and systems for labeling or barcoding molecules within one or more portions of a plurality of cells are also provided. The methods, kits, and systems may utilize photo-controlled adapter sequences, nucleic acids tags, and/or linkers.

Abstract: This disclosure relates to compounds that target microtubules, pharmaceutical compositions comprising them, and methods of using the compounds and compositions for treating diseases. More particularly, this disclosure relates to modified carbazole compounds and pharmaceutical compositions thereof, methods of targeting microtubules with these compounds, and methods of treating diseases affected by microtubule disruption.

Abstract: The present disclosure provides, among other things, helper-dependent adenoviral serotype 35 (Ad35) vectors. In various embodiments, helper-dependent Ad35 vectors can be used to deliver a therapeutic payload to a subject in need thereof. Exemplary payloads can encode replacement proteins, antibodies, CARs, TCRs, small RNAs, and genome editing systems. In certain embodiments, a helper-dependent Ad35 vector is engineered for integration of a payload into a host cell genome. The present disclosure further includes methods of gene therapy that include administration of a helper-dependent Ad35 vector to a subject in need thereof.

Abstract: Apparatuses, systems, and methods for an open-top light-sheet (OTLS) microscope which includes an illumination objective and a collection objective which have optical axes which are non-orthogonal to each other. The optical axis of the collection objective may be orthogonal to a plane of the sample holder. The illumination and collection objective may be located below the sample holder. The OTLS microscope may optionally include a second collection objective which has an optical axis orthogonal to the optical axis of the illumination objective. The illumination objective may be an air objective, and the collection objective may be an immersion objective.

Abstract: Methods and systems for measuring one or more properties of a soft material employ air transmitted ultrasound that is reflected from the soft material to generate a mechanical wave in the soft material. A method of measuring one or more properties of a soft material includes transmitting ultrasound through air to an interface boundary between the soft material and air. Force is applied to the soft material by reflecting the ultrasound from the soft material. A mechanical wave is generated in the soft material as a result of the force applied to the soft material. Propagation of the mechanical wave in the soft material is measured with an imaging system. One or more properties of the soft material is determined based on the measured propagation of the mechanical wave in the soft material.