Patents Assigned to University of Washington
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Publication number: 20230304073Abstract: Methods of uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs and/or cDNAs.Type: ApplicationFiled: April 21, 2023Publication date: September 28, 2023Applicant: UNIVERSITY OF WASHINGTONInventors: Georg SEELIG, Alexander B. ROSENBERG, Charles ROCO
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Publication number: 20230295688Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.Type: ApplicationFiled: January 17, 2023Publication date: September 21, 2023Applicant: University of WashingtonInventors: Georg Seelig, Richard Muscat, Alexander B. Rosenberg
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Publication number: 20230293053Abstract: Systems, materials, and methods for making and using in vivo screening and highly parallel tissue grafting assay arrays are described. An implantable assay array may include a biocompatible substrate. The biocompatible substrate may define a number of mutually isolated microcompartments. The number of mutually isolated microcompartments may be determined based at least in part on a size of the implantable assay array. The size of the implantable assay array may be determined based at least in part on a size of an implantation subject.Type: ApplicationFiled: August 12, 2021Publication date: September 21, 2023Applicant: UNIVERSITY OF WASHINGTONInventors: Kelly Rose Stevens, Chelsea Fortin, Colleen O'Connor
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Publication number: 20230272462Abstract: Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid in a sample are described. In an embodiment, the kit comprises a loop primer nucleic acid molecule configured for loop-mediated isothermal amplification (LAMP), the loop primer nucleic acid molecule comprising: a targeting sequence complementary to a target portion of a target nucleic acid sequence; and an adapter sequence; a displacement nucleic acid probe comprising: a fluorophore adapter sequence; and the adapter sequence; and a fluorophore adapter complement nucleic acid molecule complementary to the fluorophore adapter sequence, wherein the fluorophore adapter sequence or the fluorophore adapter complement nucleic acid molecule is coupled to a fluorophore. In an embodiment, the system comprises a thermal subsystem for heating a sample disposed therein, and an optical subsystem for optically excited the sample and detecting light emitted from the sample.Type: ApplicationFiled: July 9, 2021Publication date: August 31, 2023Applicant: UNIVERSITY OF WASHINGTONInventors: Enos Kline, Michael Roller, Robert G. Atkinson, Nuttada Panpradist, Daniel Leon, Ian Hull, Qin Wang, Barry R. Lutz
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Publication number: 20230271011Abstract: Systems and methods for high frequency epidural stimulation to control sensation are disclosed herein. In one embodiment, a method for restoring physiological function in a subject having a neurological impairment includes generating a stimulation pattern. The stimulation pattern includes pulses at a first frequency, where the pulses of the stimulation pattern are modulated by a modulation pattern at a second frequency. The second frequency is higher than the first frequency. The method also includes stimulating a region of the spinal cord with the stimulation pattern via one or more electrodes.Type: ApplicationFiled: June 2, 2021Publication date: August 31, 2023Applicants: UNIVERSITY OF WASHINGTONInventors: Chet T. MORTIZ, Abed KHORASANI, Soshi SAMEJIMA, Nicholas TOLLEY
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Publication number: 20230270677Abstract: The present disclosure describes simple, stable, and scalable antiviral therapeutic agent compositions that transform short-acting antiviral (e.g., anti-HIV) therapeutic agents that would otherwise require daily short-acting oral administration into long-acting injectable forms that lasts for many weeks per administration. A mixture of water-soluble and water-insoluble antiviral therapeutic agents can be present in the long-acting and drug-combination composition.Type: ApplicationFiled: January 7, 2021Publication date: August 31, 2023Applicant: University of WashingtonInventor: Rodney J.Y. Ho
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Publication number: 20230263817Abstract: The current disclosure provides use of high dose nicotinamide adenine dinucleotide (NAD) precursor regimens for reduction of inflammation in human patients with preexisting inflammation. The NAD precursor can be nicotinamide riboside (NR) and the high dose regimen can include at least 1000 or 2000 mg/day for at least 9 days.Type: ApplicationFiled: August 27, 2021Publication date: August 24, 2023Applicant: University of WashingtonInventors: Rong Tian, Dennis Ding-Hwa Wang, Bo Zhou, Kevin Douglas O'Brien
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Publication number: 20230265377Abstract: Devices and methods configured to determine characteristics of tissue specimens are provided. Representative tissue analysis devices include a sensing module and a reference module. The sensing module includes a first post and a second post configured to have the tissue specimen affixed thereto, and a displacement sensor configured to output a displacement signal corresponding to a displacement of the first post. The reference module includes a reference sensor configured to output a reference signal corresponding to a reference input such as an ambient magnetic field. The devices further include instructions that determine: a displacement value based upon the displacement signal; a reference value based upon the reference signal; a reference-canceled displacement value based upon the displacement value and the reference value; and a characteristic of the tissue specimen based upon the reference-canceled displacement value.Type: ApplicationFiled: October 7, 2020Publication date: August 24, 2023Applicant: University of WashingtonInventors: Nathan J. Sniadecki, Ty Higashi, Daniel Moskowitz, Jevne Micheau-Cunningham, Robert Bruce Darling
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Publication number: 20230265487Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.Type: ApplicationFiled: March 23, 2023Publication date: August 24, 2023Applicant: University of WashingtonInventors: Georg Seelig, Richard Muscat, Alexander B. Rosenberg
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Publication number: 20230250393Abstract: Described herein are methods and compositions for reducing or preventing arrhythmias associated with or caused by transplantation of cardiomyocytes to cardiac tissue. In particular embodiments, the pre-treatment of in vitro-differentiated cardiomyocytes with amiodarone before administration to cardiac tissue for engraftment reduces or prevents engraftment arrhythmias, and/or reduces the need for adjunctive anti-arrhythmia drugs after cell administration.Type: ApplicationFiled: January 20, 2023Publication date: August 10, 2023Applicant: University of WashingtonInventors: Charles E. MURRY, William Robb MACLELLAN, Robert Scott THIES, Kenta NAKAMURA
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Publication number: 20230252913Abstract: A thermally representative phantom mammalian manikin assembly is provided. The assembly can include a cover portion representative of mammalian tissue partially enclosing a thermal cavity having a thermally representative material therein. The cover portion can have an opening with a cap extending across the opening and configured to further enclose the thermal cavity. The cap can have an aperture extending therethrough to receive a device extending through the aperture and into the thermally representative material within the thermal cavity. The device can be a thermocouple configured to measure a temperature of the thermally representative material. In assemblies representing mammalian limbs, the assembly can further include a second thermal cavity representing an additional portion of the limb, e.g., thermally representative upper and lower leg cavities.Type: ApplicationFiled: February 10, 2023Publication date: August 10, 2023Applicant: University of WashingtonInventors: Shahram Aarabi, Jason Germany, Abhijith Shasheendra, Ketan Sunil Mhetre
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Publication number: 20230251258Abstract: Methods and systems for improved labeling and/or de-labeling a molecule or cell in the context of scientific experimentation, industrial applications, and clinical investigation, including the means to repeat the process of labeling and de-labeling in an efficient manner.Type: ApplicationFiled: April 20, 2023Publication date: August 10, 2023Applicant: University of WashingtonInventors: Daniel T. Chiu, Chun-Ting Kuo, Li Wu
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Publication number: 20230227891Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.Type: ApplicationFiled: January 24, 2023Publication date: July 20, 2023Applicant: University of WashingtonInventors: Georg Seelig, Richard Muscat, Alexander B. Rosenberg
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Publication number: 20230228722Abstract: Apparatus and methods for determining a concentration of one or more halogen-containing ions in a sample, including but not limited to: soils, aquifers, groundwater, drinking water, soil, tissue, blood, sewage sludge, compost, and landfill leachate, Particularly, the apparatus and processes are used for the destruction of per- and polyfluoroalkyl substances (PFAS), per- and polyfluorocarbons (PFCs), pesticides, munitions, 1,4-dioxane, pharmaceuticals, microplastics, and others. High destruction efficiencies of these substances is desirable for determination of compliance with government regulations. Apparatus include batch- and continuous-type reactor systems. Processes include supercritical water oxidation (SCWO) and hydrothermal alkaline treatments (HALT).Type: ApplicationFiled: April 26, 2021Publication date: July 20, 2023Applicant: UNIVERSITY OF WASHINGTONInventor: Brian Pinkard
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Publication number: 20230225612Abstract: In some embodiments, techniques for using machine learning to enable visible light pupilometry are provided. In some embodiments, a smartphone may be used to create a visible light video recording of a pupillary light reflex (PLR). A machine learning model may be used to detect a size of a pupil in the video recording over time, and the size over time may be presented to a clinician. In some embodiments, a system that includes a smartphone and a box that holds the smartphone in a predetermined relationship to a subject's face is provided. In some embodiments, a sequential convolutional neural network architecture is used. In some embodiments, a fully convolutional neural network architecture is used.Type: ApplicationFiled: March 20, 2023Publication date: July 20, 2023Applicant: University of WashingtonInventors: Lynn B. McGrath, Anthony Law, Randall Bly, Shwetak N. Patel, Alex T. Mariakakis, Jacob Baudin
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Publication number: 20230213360Abstract: A capacitive sensor including an electrically conductive material, and a single electrode applied with positive potential, wherein the distance between the single electrode and the electrically conductive material determines the spherical radius for a proximity sensing range.Type: ApplicationFiled: December 29, 2022Publication date: July 6, 2023Applicant: University of WashingtonInventors: Jae-Hyun Chung, Sang-Gyeun Ahn, Tianyi Li, Zhongjie Qian, Vigneshwar Sakthivelpathi
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Publication number: 20230210816Abstract: Methods for inhibiting or treating viral infection in a subject infected with a vims of the arenaviridae family with a therapeutic agent combination: (a) arbidol and aripiprazole; (b) arbidol and amodiaquine; (c) arbidol and sertraline; (d) arbidol, iprazole, and amodiaquine; (e) arbidol, aripiprazole, and sertraline; or (f) aripiprazole and amodiaquine; or pharmaceutically acceptable salts thereof.Type: ApplicationFiled: May 24, 2021Publication date: July 6, 2023Applicants: UNIVERSITY OF WASHINGTON, UNIVERSITY OF VIRGINIA PATENT FOUNDATIONInventors: Stephen J. Polyak, Shawn Herring, Jessica Wagoner, Judith White
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Publication number: 20230203462Abstract: Provided are compositions and methods for the production of modified endonucleases such as CRISPR/Cas9 system with reduced off-target activity. Methods of editing of polynucleotides using the modified endonucleases in vitro and in vivo are also disclosed. In one aspect, the disclosure provides a modified endonuclease, comprising an endonuclease and one or more mixed charge moieties covalently linked to the endonuclease, wherein each mixed charge moiety comprises about 10 to about 400 positively charged moieties and about 10 to about 400 negatively charged moieties, and wherein the ratio of the number of positively charged moieties to the number of negatively charged moieties is from about 1 :0.5 to about 1 :2.Type: ApplicationFiled: October 9, 2020Publication date: June 29, 2023Applicant: UNIVERSITY OF WASHINGTONInventors: Zhedfan Yuan, Shaoyi Jiang, Yanjiao Han, Sijin Luozhong
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Publication number: 20230201618Abstract: Methods for inducing cell death by magneto-endosomalytic therapy using magnetic nano articles. The methods include inducing cell death, inducing cell death in a subject, and application of the methods to killing tumor cells in a subject.Type: ApplicationFiled: May 21, 2021Publication date: June 29, 2023Applicant: UNIVERSITY OF WASHINGTONInventor: Xiaohu Gao
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Publication number: 20230203082Abstract: Reagents, methods, and kits for assaying enzymes associated with lysosomal storage diseases MPS-I, MPS-II, MPS-IIIA, MPS-IIIB, MPS-IVA, MPS-VI, and MPS VII.Type: ApplicationFiled: February 22, 2023Publication date: June 29, 2023Applicant: University of Washington through its Center for CommercializationInventors: Michael H. Gelb, Arun Babu Kumar, Frances Hocutt, Zdenek Spacil, Mariana Natali Barcenas Rodriguez, Frantisek Turecek, C. Ronald Scott