Patents Assigned to University of Washington
  • Publication number: 20230304073
    Abstract: Methods of uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs and/or cDNAs.
    Type: Application
    Filed: April 21, 2023
    Publication date: September 28, 2023
    Applicant: UNIVERSITY OF WASHINGTON
    Inventors: Georg SEELIG, Alexander B. ROSENBERG, Charles ROCO
  • Publication number: 20230295688
    Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.
    Type: Application
    Filed: January 17, 2023
    Publication date: September 21, 2023
    Applicant: University of Washington
    Inventors: Georg Seelig, Richard Muscat, Alexander B. Rosenberg
  • Publication number: 20230293053
    Abstract: Systems, materials, and methods for making and using in vivo screening and highly parallel tissue grafting assay arrays are described. An implantable assay array may include a biocompatible substrate. The biocompatible substrate may define a number of mutually isolated microcompartments. The number of mutually isolated microcompartments may be determined based at least in part on a size of the implantable assay array. The size of the implantable assay array may be determined based at least in part on a size of an implantation subject.
    Type: Application
    Filed: August 12, 2021
    Publication date: September 21, 2023
    Applicant: UNIVERSITY OF WASHINGTON
    Inventors: Kelly Rose Stevens, Chelsea Fortin, Colleen O'Connor
  • Publication number: 20230272462
    Abstract: Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid in a sample are described. In an embodiment, the kit comprises a loop primer nucleic acid molecule configured for loop-mediated isothermal amplification (LAMP), the loop primer nucleic acid molecule comprising: a targeting sequence complementary to a target portion of a target nucleic acid sequence; and an adapter sequence; a displacement nucleic acid probe comprising: a fluorophore adapter sequence; and the adapter sequence; and a fluorophore adapter complement nucleic acid molecule complementary to the fluorophore adapter sequence, wherein the fluorophore adapter sequence or the fluorophore adapter complement nucleic acid molecule is coupled to a fluorophore. In an embodiment, the system comprises a thermal subsystem for heating a sample disposed therein, and an optical subsystem for optically excited the sample and detecting light emitted from the sample.
    Type: Application
    Filed: July 9, 2021
    Publication date: August 31, 2023
    Applicant: UNIVERSITY OF WASHINGTON
    Inventors: Enos Kline, Michael Roller, Robert G. Atkinson, Nuttada Panpradist, Daniel Leon, Ian Hull, Qin Wang, Barry R. Lutz
  • Publication number: 20230271011
    Abstract: Systems and methods for high frequency epidural stimulation to control sensation are disclosed herein. In one embodiment, a method for restoring physiological function in a subject having a neurological impairment includes generating a stimulation pattern. The stimulation pattern includes pulses at a first frequency, where the pulses of the stimulation pattern are modulated by a modulation pattern at a second frequency. The second frequency is higher than the first frequency. The method also includes stimulating a region of the spinal cord with the stimulation pattern via one or more electrodes.
    Type: Application
    Filed: June 2, 2021
    Publication date: August 31, 2023
    Applicants: UNIVERSITY OF WASHINGTON
    Inventors: Chet T. MORTIZ, Abed KHORASANI, Soshi SAMEJIMA, Nicholas TOLLEY
  • Publication number: 20230270677
    Abstract: The present disclosure describes simple, stable, and scalable antiviral therapeutic agent compositions that transform short-acting antiviral (e.g., anti-HIV) therapeutic agents that would otherwise require daily short-acting oral administration into long-acting injectable forms that lasts for many weeks per administration. A mixture of water-soluble and water-insoluble antiviral therapeutic agents can be present in the long-acting and drug-combination composition.
    Type: Application
    Filed: January 7, 2021
    Publication date: August 31, 2023
    Applicant: University of Washington
    Inventor: Rodney J.Y. Ho
  • Publication number: 20230263817
    Abstract: The current disclosure provides use of high dose nicotinamide adenine dinucleotide (NAD) precursor regimens for reduction of inflammation in human patients with preexisting inflammation. The NAD precursor can be nicotinamide riboside (NR) and the high dose regimen can include at least 1000 or 2000 mg/day for at least 9 days.
    Type: Application
    Filed: August 27, 2021
    Publication date: August 24, 2023
    Applicant: University of Washington
    Inventors: Rong Tian, Dennis Ding-Hwa Wang, Bo Zhou, Kevin Douglas O'Brien
  • Publication number: 20230265377
    Abstract: Devices and methods configured to determine characteristics of tissue specimens are provided. Representative tissue analysis devices include a sensing module and a reference module. The sensing module includes a first post and a second post configured to have the tissue specimen affixed thereto, and a displacement sensor configured to output a displacement signal corresponding to a displacement of the first post. The reference module includes a reference sensor configured to output a reference signal corresponding to a reference input such as an ambient magnetic field. The devices further include instructions that determine: a displacement value based upon the displacement signal; a reference value based upon the reference signal; a reference-canceled displacement value based upon the displacement value and the reference value; and a characteristic of the tissue specimen based upon the reference-canceled displacement value.
    Type: Application
    Filed: October 7, 2020
    Publication date: August 24, 2023
    Applicant: University of Washington
    Inventors: Nathan J. Sniadecki, Ty Higashi, Daniel Moskowitz, Jevne Micheau-Cunningham, Robert Bruce Darling
  • Publication number: 20230265487
    Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.
    Type: Application
    Filed: March 23, 2023
    Publication date: August 24, 2023
    Applicant: University of Washington
    Inventors: Georg Seelig, Richard Muscat, Alexander B. Rosenberg
  • Publication number: 20230250393
    Abstract: Described herein are methods and compositions for reducing or preventing arrhythmias associated with or caused by transplantation of cardiomyocytes to cardiac tissue. In particular embodiments, the pre-treatment of in vitro-differentiated cardiomyocytes with amiodarone before administration to cardiac tissue for engraftment reduces or prevents engraftment arrhythmias, and/or reduces the need for adjunctive anti-arrhythmia drugs after cell administration.
    Type: Application
    Filed: January 20, 2023
    Publication date: August 10, 2023
    Applicant: University of Washington
    Inventors: Charles E. MURRY, William Robb MACLELLAN, Robert Scott THIES, Kenta NAKAMURA
  • Publication number: 20230252913
    Abstract: A thermally representative phantom mammalian manikin assembly is provided. The assembly can include a cover portion representative of mammalian tissue partially enclosing a thermal cavity having a thermally representative material therein. The cover portion can have an opening with a cap extending across the opening and configured to further enclose the thermal cavity. The cap can have an aperture extending therethrough to receive a device extending through the aperture and into the thermally representative material within the thermal cavity. The device can be a thermocouple configured to measure a temperature of the thermally representative material. In assemblies representing mammalian limbs, the assembly can further include a second thermal cavity representing an additional portion of the limb, e.g., thermally representative upper and lower leg cavities.
    Type: Application
    Filed: February 10, 2023
    Publication date: August 10, 2023
    Applicant: University of Washington
    Inventors: Shahram Aarabi, Jason Germany, Abhijith Shasheendra, Ketan Sunil Mhetre
  • Publication number: 20230251258
    Abstract: Methods and systems for improved labeling and/or de-labeling a molecule or cell in the context of scientific experimentation, industrial applications, and clinical investigation, including the means to repeat the process of labeling and de-labeling in an efficient manner.
    Type: Application
    Filed: April 20, 2023
    Publication date: August 10, 2023
    Applicant: University of Washington
    Inventors: Daniel T. Chiu, Chun-Ting Kuo, Li Wu
  • Publication number: 20230227891
    Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.
    Type: Application
    Filed: January 24, 2023
    Publication date: July 20, 2023
    Applicant: University of Washington
    Inventors: Georg Seelig, Richard Muscat, Alexander B. Rosenberg
  • Publication number: 20230228722
    Abstract: Apparatus and methods for determining a concentration of one or more halogen-containing ions in a sample, including but not limited to: soils, aquifers, groundwater, drinking water, soil, tissue, blood, sewage sludge, compost, and landfill leachate, Particularly, the apparatus and processes are used for the destruction of per- and polyfluoroalkyl substances (PFAS), per- and polyfluorocarbons (PFCs), pesticides, munitions, 1,4-dioxane, pharmaceuticals, microplastics, and others. High destruction efficiencies of these substances is desirable for determination of compliance with government regulations. Apparatus include batch- and continuous-type reactor systems. Processes include supercritical water oxidation (SCWO) and hydrothermal alkaline treatments (HALT).
    Type: Application
    Filed: April 26, 2021
    Publication date: July 20, 2023
    Applicant: UNIVERSITY OF WASHINGTON
    Inventor: Brian Pinkard
  • Publication number: 20230225612
    Abstract: In some embodiments, techniques for using machine learning to enable visible light pupilometry are provided. In some embodiments, a smartphone may be used to create a visible light video recording of a pupillary light reflex (PLR). A machine learning model may be used to detect a size of a pupil in the video recording over time, and the size over time may be presented to a clinician. In some embodiments, a system that includes a smartphone and a box that holds the smartphone in a predetermined relationship to a subject's face is provided. In some embodiments, a sequential convolutional neural network architecture is used. In some embodiments, a fully convolutional neural network architecture is used.
    Type: Application
    Filed: March 20, 2023
    Publication date: July 20, 2023
    Applicant: University of Washington
    Inventors: Lynn B. McGrath, Anthony Law, Randall Bly, Shwetak N. Patel, Alex T. Mariakakis, Jacob Baudin
  • Publication number: 20230213360
    Abstract: A capacitive sensor including an electrically conductive material, and a single electrode applied with positive potential, wherein the distance between the single electrode and the electrically conductive material determines the spherical radius for a proximity sensing range.
    Type: Application
    Filed: December 29, 2022
    Publication date: July 6, 2023
    Applicant: University of Washington
    Inventors: Jae-Hyun Chung, Sang-Gyeun Ahn, Tianyi Li, Zhongjie Qian, Vigneshwar Sakthivelpathi
  • Publication number: 20230210816
    Abstract: Methods for inhibiting or treating viral infection in a subject infected with a vims of the arenaviridae family with a therapeutic agent combination: (a) arbidol and aripiprazole; (b) arbidol and amodiaquine; (c) arbidol and sertraline; (d) arbidol, iprazole, and amodiaquine; (e) arbidol, aripiprazole, and sertraline; or (f) aripiprazole and amodiaquine; or pharmaceutically acceptable salts thereof.
    Type: Application
    Filed: May 24, 2021
    Publication date: July 6, 2023
    Applicants: UNIVERSITY OF WASHINGTON, UNIVERSITY OF VIRGINIA PATENT FOUNDATION
    Inventors: Stephen J. Polyak, Shawn Herring, Jessica Wagoner, Judith White
  • Publication number: 20230203462
    Abstract: Provided are compositions and methods for the production of modified endonucleases such as CRISPR/Cas9 system with reduced off-target activity. Methods of editing of polynucleotides using the modified endonucleases in vitro and in vivo are also disclosed. In one aspect, the disclosure provides a modified endonuclease, comprising an endonuclease and one or more mixed charge moieties covalently linked to the endonuclease, wherein each mixed charge moiety comprises about 10 to about 400 positively charged moieties and about 10 to about 400 negatively charged moieties, and wherein the ratio of the number of positively charged moieties to the number of negatively charged moieties is from about 1 :0.5 to about 1 :2.
    Type: Application
    Filed: October 9, 2020
    Publication date: June 29, 2023
    Applicant: UNIVERSITY OF WASHINGTON
    Inventors: Zhedfan Yuan, Shaoyi Jiang, Yanjiao Han, Sijin Luozhong
  • Publication number: 20230201618
    Abstract: Methods for inducing cell death by magneto-endosomalytic therapy using magnetic nano articles. The methods include inducing cell death, inducing cell death in a subject, and application of the methods to killing tumor cells in a subject.
    Type: Application
    Filed: May 21, 2021
    Publication date: June 29, 2023
    Applicant: UNIVERSITY OF WASHINGTON
    Inventor: Xiaohu Gao
  • Publication number: 20230203082
    Abstract: Reagents, methods, and kits for assaying enzymes associated with lysosomal storage diseases MPS-I, MPS-II, MPS-IIIA, MPS-IIIB, MPS-IVA, MPS-VI, and MPS VII.
    Type: Application
    Filed: February 22, 2023
    Publication date: June 29, 2023
    Applicant: University of Washington through its Center for Commercialization
    Inventors: Michael H. Gelb, Arun Babu Kumar, Frances Hocutt, Zdenek Spacil, Mariana Natali Barcenas Rodriguez, Frantisek Turecek, C. Ronald Scott