Abstract: Examples of active millimeter-wave imaging systems are described which may utilize modulation schemes to provide illumination signals. The use of modulation techniques may allow for the use of direct-conversion receivers while retaining an ability to separate desired received signal from self-jamming and/or DC offset signal(s) generated by the direct-conversion receivers. In some examples, modulation schemes include the use of balanced orthogonal codes which may support MIMO or massive MIMO imaging systems.

Abstract: Disclosed herein are macrocyclic polypeptides having no more than 3 ammo acid substitutions compared to the amino acid sequence of any one of SEQ ID NO: 1-2.37 or a mirror image thereof, wherein the polypeptide includes both L and D amino acids, libraries of such polypeptides, and uses thereof.

Abstract: The present disclosure features a composition, including molecularly imprinted crosslinked polymers that have been imprinted with trimethylamine N-oxide. The molecularly imprinted crosslinked polymers have specific binding sites for trimethylamine N-oxide, and a trimethylamine N-oxide absorption capacity of at least 0.5 mg/g.

Abstract: The present disclosure features a luminescent molecule, including a luminophore (e.g., a fluorescent dye); and a moiety including a heteroaryl core covalently and directly bonded to the luminophore. The luminescent molecule has an increased photoluminescence quantum yield relative to an analogous luminophore without a covalently bonded moiety including the heteroaryl core.

Abstract: The present disclosure provides a method of making an article, including: providing a composition comprising two or more types of polymerizable monomers and two or more types of polymerization initiators; exposing the build region to one or more polymerization stimuli; polymerizing the two or more polymerizable monomers at the build region to provide a polymer layer; and advancing the polymer layer away from the build region to provide a three-dimensional article containing two or more integrally mixed polymers.

Abstract: Systems and methods are provided for fabricating microneedle arrays that includes electrospun fibers preferentially disposed within the microneedles of the array. Providing the electrospun fibers preferentially in the microneedles allows for more of a drug or other substance present in the fibers to be deposited into tissue or to provide other benefits. A mold for forming the microneedle arrays includes an insulating surface layer. The insulating surface layer affects the electric field during electrospinning such that electrospun fibers are deposited preferentially within the microneedle cavities of the mold relative to the surface of the mold. A bulk material can then be applied to the mold to form the bulk of the microneedles with electrospun fibers embedded within and a backing layer to which the microneedles are attached.

Abstract: Crosslinked films having electro-optic activity, compositions and compounds for making the films, methods for making the films, and devices that include the films are disclosed.

Abstract: Systems and methods to selectively protect therapeutic cells by reducing CD33 expression in the therapeutic cells using base editors and targeting non-therapeutic cells with an anti-CD33 therapy are described. The selective protection results in the enrichment of the therapeutic cells while simultaneously targeting any diseased, malignant and/or non-therapeutic CD33 expressing cells within a subject.

Abstract: Free-standing non-fouling polymers and polymeric compositions, monomers and macromonomers for making the polymers and polymeric compositions, objects made from the polymers and polymeric compositions, and methods for making and using the polymers and polymeric compositions.

Abstract: An apparatus, system, and method for filtering and assaying a fluid sample are described. In an embodiment, the apparatus includes a filtration unit comprising: a filter bracket shaped to removably couple with a fluid sample cup and a vacuum container; and a filter housing cooperatively couplable to the filter bracket and comprising a filter configured to filter fluid passing through the filter bracket; and an assay device shaped to cooperatively couple with the filter housing and comprising a porous matrix positioned to be in fluidic communication with the filter when the filter housing is cooperatively coupled with the assay device.

Abstract: The invention provides methods for preparing DNA sequencing libraries by assembling short read sequencing data into longer contiguous sequences for genome assembly, full length cDNA sequencing, metagenomics, and the analysis of repetitive sequences of assembled genomes.

Abstract: Disclosed are general and “substantially pure” branched discrete polyethylene glycol constructs useful in attaching to a variety of biologically active groups, for example, preferential locators, as well as biologics like enzymes, for use in diagnostics, e.g. imaging, therapeutics, theranostics, and moieties specific for other applications. In its simplest intermediate state, a branched discrete polyethylene glycol construct is terminated at one end by a chemically reactive moiety, “A”, a group that is reactive with a biologic material that creates “A”, which is a biologically reactive group, connected through to a branched core (BC) which has attached at least two dPEG-containing chains, indicated by the solid line, , having terminal groups, which can be charged, non-reactive or reactable moieties and containing between about 2 and 64 dPEG residues.

Abstract: In some embodiments, a computer-implemented method of determining an identity of one or more monomer subunit residues of a polymer analyte is provided, In some embodiments, a raw current signal generated by using a variable voltage to translocate the polymer analyte through a nanopore. In some embodiments, change points are detected in the raw current signal to determine a series of states, In some embodiments, capacitance compensation is performed on the raw current signal for each state to create an ionic current-vs-voltage curve for each state. In some embodiments, the ionic current-vs-voltage curves is converted to conductance-vs-voltage curves. In some embodiments, filtering is performed for the series of states to create a series of filtered states. In some embodiments, the identity of one or more monomer subunit residues of the polymer analyte is determined based on the series of filtered states.

Abstract: Devices, systems, and methods for trapping and manipulating portions of tissue are described. In an embodiment, the devices include an array of traps, wherein traps of the array of traps are shaped to trap a tissue sample; and a well is in registry and fluidic communication with a trap of the array of traps.

Abstract: Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes nucleic acids that represent the chromatin accessibility from the plurality of single cells. In one embodiment, the nucleic acids include three index sequences. In another embodiment, the present disclosure provides methods for characterizing rare events in isolated cells and nuclei.

Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

Abstract: Reagents and methods for labeling biomolecules with astatine isotopes in higher oxidation states. The reagents and methods allow for efficient labeling of biomolecules, such as antibodies, without the formation of high molecular weight by products that arise due to 211At-promoted dimerization and higher aggregation of the conjugated biomolecules, and diminish cellular retention of astatine caused by cellular oxidization of the bonded astatine atom in vivo.

Abstract: By monitoring pressure transients in a liquid within a liquid distribution system using only a single sensor, events such as the opening and closing of valves at specific fixtures are readily detected. The sensor, which can readily be coupled to a faucet bib, transmits an output signal to a computing device. Each such event can be identified by the device based by comparing characteristic features of the pressure transient waveform with previously observed characteristic features for events in the system. These characteristic features, which can include the varying pressure, derivative, and real Cepstrum of the pressure transient waveform, can be used to select a specific fixture where a valve open or close event has occurred. Flow to each fixture and leaks in the system can also be determined from the pressure transient signal. A second sensor disposed at a point disparate from the first sensor provides further event information.

Abstract: Pharmaceutical compositions including a mitochondria-targeting polypeptide and a therapeutic agent coupled to the mitochondria-targeting polypeptide are provided. Methods of treating a subject in need thereof including administering a therapeutically effective amount of the pharmaceutical composition to the subject are provided. The subject may have an aging-related condition, heart failure, diabetes, myocardial infarction, acquired mitochondrial disorder, and/or inherited mitochondrial disorder. Methods of delivering compound to a mitochondrion and of preparing a compound for delivery to a mitochondrion are also provided. Furthermore, biological tracers including a mitochondria-targeting polypeptide and a label coupled to the mitochondria-targeting polypeptide are provided.

Abstract: Provided herein are compositions and methods for identifying or quantitating one or more analytes in sample. The composition can comprise an affinity molecule reversibly conjugated to a label moiety via a double-stranded nucleic acid linker or via an adaptor molecule. The affinity molecule and the label moiety can be linked to different strands of the double-stranded nucleic acid linker. Compositions can be used in any biological assays for detection, identification and/or quantification of target molecules or analytes, including multiplex staining for molecular profiling of individual cells or cellular populations. For example, the compositions can be adapted for use in immunofluorescence, fluorescence in situ hybridization, immunohistochemistry, western blot, and the like.