Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

Abstract: Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.

Abstract: Chromophores with large hyperpolarizabilities, films with electro-optic activity comprising the chromophores, and electro-optic devices comprising the chromophores are disclosed.

Abstract: A system for sanding a surface includes a sanding tool, a robotic manipulator to move the sanding tool relative to the surface, and a control unit operatively coupled with the sanding tool and the robotic manipulator. The control unit is operable to: (1) move the sanding tool to a sanding position relative to the surface in which an abrasive surface is in contact with the surface and a sanding force is approximately normal to the surface; (2) set one or more sanding parameters corresponding to a model material removal rate; (3) monitor one or more of the sanding parameters; (4) determine an actual material removal rate, based on one or more of the sanding parameters being monitored; and (5) modify one or more of the sanding parameters until the actual material removal rate is approximately equal to the model material removal rate.

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand.

Abstract: Free-standing non-fouling polymers and polymeric compositions, monomers and macromonomers for making the polymers and polymeric compositions, objects made from the polymers and polymeric compositions, and methods for making and using the polymers and polymeric compositions

Abstract: A method for modulating the piasmonic resonance of a noble metal nanoparticle to enhance the luminescence of an oxygen sensitive dye; an oxygen sensitive composition that includes a nanostructure comprising a noble metal particle and an oxygen sensitive dye: a substrate having a surface coated with the oxygen sensitive composition; methods and sensors for determining oxygen concentration using the oxygen sensitive composition.

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand.

Abstract: In some embodiments, techniques for using machine learning to enable visible light pupilometry are provided. In some embodiments, a smartphone may be used to create a visible light video recording of a pupillary light reflex (PLR). A machine learning model may be used to detect a size of a pupil in the video recording over time, and the size over time may be presented to a clinician. In some embodiments, a system that includes a smartphone and a box that holds the smartphone in a predetermined relationship to a subject's face is provided. In some embodiments, a sequential convolutional neural network architecture is used. In some embodiments, a fully convolutional neural network architecture is used.

Abstract: A novel range of non-shearing axial auxetic structures are disclosed having useful functions and properties. The novel non-shearing axial auxetic structures have applications including compliant universal joints, structures with switchable Poisson ratios, nd multiple discrete dimensional configurations. Further, combination structures of multiple individual non-shearing axial auxetic structures having useful functions and properties are also disclosed.

Abstract: The present disclosure describes an injectable aqueous dispersion, including an aqueous solvent, and a chemotherapeutic agent composition dispersed in the aqueous solvent to provide the injectable aqueous dispersion. The chemotherapeutic agent composition includes a combination of chemotherapeutic agents selected from: gemcitabine and paclitaxel; and venetoclax and zanubrutinib. The chemotherapeutic agent composition further includes one or more compatibilizers comprising a lipid (e.g., a lipid excipient), a lipid conjugate, or a combination thereof. The chemotherapeutic agents of the chemotherapeutic agent composition exhibit a synergistic chemotherapeutic effect.

Abstract: In some embodiments, techniques capable of obtaining high-quality fetal ECG (fECG) information using as few as two composite (maternal and fetal) maternal abdominal ECG (aECG) signals to derive the fetal ECG information are provided. In some embodiments, maternal ECG information and fetal ECG information are both obtained from the aECG signals, and are either presented or stored. The techniques may use novel proposed diffusion-based channel selection criteria. To validate the proposed techniques, analysis results of two publicly available databases are described, and compared with other available techniques in the literature.

Abstract: Methods and systems for tuning lithotripsy frequency to target size are disclosed. In one embodiment, a lithotripsy system for comminuting a stone in a body includes: a burst wave lithotripsy (BWL) therapy transducer configured to transmit smooth ultrasound waves within a burst of ultrasound waves toward the stone; and a controller configured to determine operating frequency of the ultrasound waves of the therapy transducer. The operating frequency of the ultrasound waves is determined as: f = Const . c d where: d is a diameter of the stone, f is the frequency of the ultrasound waves, c is a wave speed in the stone, and Const. is a predetermined constant.

Abstract: A wireless power transfer system includes a transmitter configured to transmit power to a receiver, for example, through coupled resonators. The transmitter receives feedback from the receiver, and uses the feedback to control the power transmission, to control a parameter at the receiver, for example, a rectified voltage output by the receiver. The feedback to the transmitter may be provided, for example, by an out-of-band radio system between the transmitter and receiver, by a reflection coefficient at the transmitter, and/or by an encoded modulation of power in the receiver, for example, in an impedance matching module. The transmitter may control the transmitted power, for example, by controlling a transmitter signal generator voltage (VSIG), a transmitter gate driver voltage (VGD), a transmitter amplifier voltage (VPA), and/or an impedance setting in a transmitter impedance matching module.

Abstract: Disclosed herein are compositions and methods for their use in treating dental disorders, whitening teeth, restoring and retaining the tooth structure and restoring the mineral content of the tooth-mineral loss due to demineralization, wherein the compositions include a first formulation that includes a polypeptide effective to treat a dental disorder and/or whiten teeth, and one or more further formulations including at least one calcium ion source and/or at least one phosphate ion source, wherein the first formulation is configured to release the polypeptide more rapidly than Ca2+ is released from the at least one calcium ion source and PO43? is released from the at least one phosphate ion source.

Abstract: Reagents, methods, and kits for assaying enzymes associated with lysosomal storage diseases MPS-I, MPS-II, MPS-IIIA, MPS-IIIB, MPS-IVA, MPS-VI, and MPS VII.

Abstract: Systems and methods for promoting neuroplasticity in a brain of a subject to improve and/or restore neural function are disclosed herein. One such method includes detecting residual movement and/or muscular activity in a limb of the subject, such as a paretic limb. The method further includes generating a stimulation pattern based on the detected movement and/or muscular activity, and stimulating the brain of the subject with the stimulation pattern. It is expected that delivering stimulation based on the detected residual movement and/or muscular activity of the limb will induce neuroplasticity for restoring neural function, such as control of the limb. A second method involves detecting brain signals and delivering contingent stimulation. A third method involves delivering pairs of successive stimulus patterns to two brain sites, controlled either by preprogrammed sequences or contingent on neural or muscular activity or movement.

Abstract: The present disclosure describes an immunotherapy delivery hydrogel system. The immunotherapy delivery hydrogel system can be degradable and can release therapeutic agents at a tunable rate, and in a controlled manner. The immunotherapy delivery hydrogel system includes a hydrogel matrix and cancer therapeutic agent(s) associated with the hydrogel matrix. The hydrogel system can further include tumor cell-attractant(s) conjugated to the hydrogel matrix. The tumor cell-attractant(s) and the cancer therapeutic agent(s) act synergistically to treat cancer.

Abstract: Photosensitizers are incorporated into articles, such a personal protective equipment. A method of applying continuous and consistent light includes fitting the articles with light sources and optical fibers to apply light to the areas of the articles incorporated with the photosensitizers. Photosensitizers can be applied to articles by various applicators in either a gel or solution. A gel can be particularly effective when used on hydrophobic surfaces. Photodynamic reactor systems can be used to determine the effective doses of photosensitizers and the light dosimetry which can then be applied for use with the articles.

Abstract: Described herein, inter alia, are certain substituted imidazo[1,5-a]pyrazines of formula (I) and methods of using the same for modulating the activity of Ire1.