Abstract: A method of producing a polyunsaturated fatty acid in a transgenic bacterium, includes: introducing a recombinant plasmid into a bacteria cell to obtain the transgenic bacterium, wherein the recombinant plasmid comprises a polynucleotide encoding one or more elongase and one or more desaturase; incubating the transgenic bacterium in a medium containing a fatty acid substrate; harvesting the transgenic bacterium, and extracting the polyunsaturated fatty acid from the transgenic bacterium. A method of converting linoleic acid to arachidonic acid and a cassette and recombinant plasmid comprising nucleic acid sequences for the above methods are also provided.

Abstract: Methods and apparatus are disclosed for operating a memory cell formed from the plurality of coupled Josephson junctions. The memory cell is configured such that applying an electrical signal to the junctions can cause at least one, but not all, of the junctions to change their respective phase states. Subsequent writes to the memory cell using substantially the same electrical pulse do not change the phase state of the plurality of junctions. The memory cell can be ready by providing another electrical pulse to one of the junctions and receiving an output electrical pulse generated in response by a different Josephson junction of the memory cell. A set of phase states are selected to represent the logic values that are stable across anticipated operating conditions for the memory cell. Methods of selecting electrical parameters and manufacturing memory cells are further disclosed.

Abstract: A hybrid type iterative decoding method for a three-dimensional turbo product code (TPC) having a first axis (FA), a second axis (SA), and a third axis (TA) including: a parallel decoding step of applying a predetermined decoding algorithm (PDA) in parallel to current FA and SA input values (IVs) which are determined based on at least two previous decoding values (DVs), respectively, among the previous FA, SA and TA DVs which are generated in advance to generate a current FA DV and a current SA DV, respectively; a serial decoding step of applying PDA to a current TA IV determined based on the current FA and SA DVs to generate a current TA DV; and performing hard decision based on the current FAs DV, the current SA DV, the current TA DV, and the received signal value.

Abstract: A metastable true random number generator realized on an FPGA comprises a configurable delay chain including rough adjustment module and a fine adjustment module. The rough adjustment module comprises 32 rough adjustment cells each including a 1st 6-input lookup table and a two-to-one selector. The 1st input port of each 1st 6-input lookup table is connected to the 1st input terminal of the corresponding two-to-one selector, and the connecting terminal is the input terminal of the corresponding rough adjustment cell. The 2nd input port, the 3rd input port, the 4th input port, the 5th input port and the 6th input port of each 1st 6-input lookup table are all accessed to a low level 0. The output port of each 1st 6-input lookup table is connected to the 2nd input terminal of the corresponding two-to-one selector.

Abstract: This disclosure relates to methods of treating or preventing cystic fibrosis transmembrane conductance regulator (CFTR) mediated diseases such as cystic fibrosis, chronic obstructive pulmonary disease, chronic pancreatitis, chronic bronchitis, asthma, mucus formation, comprising administering an effective amount of a 3-(phenyl)-N-(4-phenoxybenzyl)-1,2,4-oxa-diazole-5-carboxamide compound, salt, or derivative thereof to a subject in need thereof. In certain embodiments, the 2-amino-N-benzylidene-acetohydrazide compound is 3-(3,5-dibromo-4-hydroxyphenyl)-N-(4-phenoxybenzyl)-1,2,4-oxadiazole-5-carboxamide.

Abstract: A closed system that captures energy derived from the head differential rather than open-water flows velocities while reducing potential environmental damages and costly maintenance due to bio-fouling. The continuously derived energy system utilizes an offshore bladder in communication with both a primary onshore bladder and a supplemental onshore bladder. Tidal energy is captured by turbines as fluid is transferred between the bladders. In addition, the system continuously extracts energy by diverting fluid to and from the supplemental onshore bladder during periods of near-high-ride and near-low-tide, during which the pressure differential between the offshore bladder and the primary onshore bladder becomes inefficient for energy production.

Abstract: The invention relates to methods of eliciting an immune response to group A streptococcal bacteria in a mammal, the method including the step of administering to the mammal an effective amount of a composition comprising an isolated p145 peptide of SEQ ID NO: 56 and/or a p145 peptide variant having an amino acid sequence at least 90% identical to SEQ ID NO: 56, and an isolated SpyCEP peptide of SEQ ID NO: 18 and/or a SpyCEP peptide variant having an amino acid sequence at least 90% identical to SEQ ID NO: 18.

Abstract: Embodiments of the present disclosure include particles, methods of making particles, methods of delivering an active agent using the particle, and the like.

Abstract: Disclosed are nanostructured materials that reflect light in selected spectra incorporated in dark colored textiles or substrates. In one aspect, a light reflecting material includes a textile exhibiting a dark color and formed of a plurality of fibers, and nanostructures arranged on the fibers and formed of a plurality of nanoparticles, the nanostructures having a dimension size of substantially less than ½ of a visible light wavelength, in which the nanostructures reflect light from the textile or substrate in at least one of infrared, near-infrared, or red visible light spectra.

Abstract: Provided is a measurement method whereby the amount of unbound bilirubin (UB) can be exactly reflected whether a specimen contains a large amount of conjugated bilirubin or not. The measurement method for UB according to the present invention comprises decomposition step (i), decomposition stopping step (ii), contact step (iii) and detection step (iv). In decomposition step (i), a blood sample containing unconjugated bilirubin (iD-Bil) and conjugated bilirubin (D-Bil) is subjected to an oxidative decomposition reaction of UB in iD-Bil and D-Bil. In decomposition stopping step (ii), the oxidative decomposition reaction is stopped to give a decomposition product of the sample. In contact step (iii), the decomposition product of the sample is contacted with UnaG that is capable of specifically binding to iD-Bil. Separately, an unreacted sample, which is the blood sample not subjected to decomposition step (i), is contacted with UnaG too.

Abstract: A manufacturing method includes: 1) forming a melt including one or more metals; 2) introducing nanostructures into the melt at an initial volume fraction of the nanostructures; and 3) at least partially evaporating one or more metals from the melt so as to form a metal matrix nanocomposite including the nanostructures dispersed therein at a higher volume fraction than the initial volume fraction.

Abstract: The disclosure provides a system and a method for reducing hazardous gases, including PHGs, through one or more photocatalysts in a filter system. A microstructure of the photocatalytic filter can be formed using biological systems as a template for the photocatalysts to be deposited thereon. The biological system can be removed by heat, oxidation, or by chemical processes to leave the photocatalytic template as a filter for the gases. In various embodiments, multiple photocatalysts can be activated at different wavelengths to filter different gases, or multiple photocatalysts can be activated at the same wavelength to filter different gases, or a photocatalyst can be activated at different wavelengths to filter different gases, or some combination thereof. The activation can be sequential or concurrent. For multiple layers of photocatalysts, the sequence of the photocatalysts can be arranged to reduce damaging output from an upstream photocatalyst to one or more downstream photocatalysts.

Abstract: Disclosed herein are methods and systems for controlled ejection of desired material onto surfaces including in single cells using nanopipettes, as well as ejection onto and into cells. Some embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller for depositing a user defined pattern on an arbitrary substrate for the purpose of controlled cell adhesion and growth. Alternate embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller and electronic control of a voltage differential in a bore of the nanopipette electroosmotically injecting material into a cell in a high-throughput manner and with minimal damage to the cell. Yet other embodiments are directed to method and system comprising functionalized nanopipettes combined with scanning ion conductance microscopy for studying molecular interactions and detection of biomolecules inside a single living cell.

Abstract: This invention provides compositions and methods for preventing inflammatory diseases of the joints, including rheumatoid and osteoarthritis, tendonitis, bursitis, inflammation of the ligament, synovitis, gout, and systemic lupus erythematosus, wherein the methods include injecting into the inflamed joint a therapeutic anti-inflammatory composition comprising a bacterial or viral IL-10 expression construct, wherein the IL-10 expression construct comprises a bacterial or viral backbone and a nucleic acid sequence encoding interleukin-10.

Abstract: The present invention generally relates to a method of using NMR relaxation rates (R2) of water molecules as an indicator of the extent of aggregation of biopharmaceutical formulations. The biopharmaceutical can be evaluated nondestructively without the vial or container being opened or protective seal compromised (i.e., broken). The method is applicable to all biopharmaceuticals and the water signal obtained by magnetic resonance relaxometry is very strong and sensitive because water is used as the solvent and is present in high (>90%) concentrations in every biopharmaceutical formulation.

Abstract: Disclosed are various embodiments for estimating the flow of blood through a blood vessel in a region of interest. Passage of a bolus of fluid through an imaged blood vessel over a period of time is tracked by a computing device. The computing device fits the tracked passage of the bolus of fluid to a modeled passage of the bolus of fluid. The computing device then estimates a volume of blood flow through the imaged blood vessel based at least in part on a fit of the tracked passage of the bolus of fluid to the modeled passage of the bolus of fluid.

Abstract: An apparatus for probing an interface via second harmonic generation (SHG) spectroscopy is provided.

Abstract: A flash memory structure and a method of making the same are provided. The flash memory structure comprises a substrate, a source, a drain, a tunnel isolation layer, a ferroelectric-charge-trapping layer, at least one blocking isolation layer and at least one gate. The substrate is made of a semiconductive material. The source is formed on the substrate. The drain is formed on the substrate and spaced apart from the source. The tunnel isolation layer is formed on the substrate. The ferroelectric-charge-trapping layer is formed on the tunnel isolation layer and contains a charge-trapping layer and a ferroelectric negative-capacitance effect layer. The at least one blocking isolation layer is formed on the ferroelectric-charge-trapping layer. The at least one gate is formed on the blocking isolation layer. The ferroelectric negative-capacitance effect layer is made of a material with the ferroelectric negative-capacitance effect.

Abstract: The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

Abstract: Disclosed herein are nanofibrillar materials and aerogel-like materials comprised of nanofibrils, and methods for making such materials.