Abstract: The present invention discloses a RamA transcription factor mutant for promoting the production of N-acetylglucosamine and use thereof. The mutant is obtained by mutating lysine at position 90 to asparagine and serine at position 92 to lysine in a parent having an amino acid sequence as shown in SEQ ID NO: 2. The present invention provides a genetically engineered strain that overexpresses the RamA transcription factor mutant and increases the production of N-acetylglucosamine. By overexpressing the transcription factor RamA that is involved in the regulation of carbon metabolism, the extracellular accumulation of N-acetylglucosamine is increased, with a maximum concentration reaching 31.5 g/L, which lays a foundation for further metabolic engineering of Corynebacterium glutamicum to produce glucosamine. The method for constructing recombinant Corynebacterium glutamicum of the invention is simple, and convenient in use, and thus has good application prospects.

Abstract: Systems and methods of quantum sensing include depositing a sample volume onto an ensemble of quantum defects, hyperpolarizing spins in the sample volume, performing a sensing sequence, and reading out information regarding electronic spin states of the quantum defects in the ensemble of quantum defects, which sense the hyperpolarized spins in the sample volume.

Abstract: A device and a method for fabricating a ceramic reinforced composite coating based on plasma remelting and injection. The device includes a plasma cladding assembly, a powder feeding assembly, a metal-based substrate, and a thermal infrared imager. The plasma cladding assembly comprises a plasma gun and a plasma generator. A plasma arc generated is used to heat the substrate and form a molten pool on the substrate. The powder feeding assembly comprises a powder feeder configured to feed ceramic particles to the molten pool through a powder feeding copper tube. The thermal infrared imager is configured to acquire an infrared image of the molten pool and acquire an optimal injection position of the ceramic particles according to the infrared image. The optimal injection position is a midpoint between a trailing edge of the plasma arc emitted on the substrate and a trailing edge of the molten pool.

Abstract: The present disclosure provides a method for preparing concentrated high-protein yogurt before fermentation, belonging to the technical field of dairy product processing. The method for preparing the concentrated high-protein yogurt before fermentation in the present disclosure includes: enabling a high-protein base material with a decalcification rate of 21.8-44.2% to be subjected to heat treatment at pH of 6.6-6.8 and temperature of 80-95° C. for 10-30 min; after that, cooling to 40-45° C., adding 0.05-0.2% (w/w) of a starter, and carrying out high end-point pH fermentation at 40-45° C. until the pH drops to 4.8-5.0; and then, performing low-temperature after-ripening to obtain the high-protein yogurt. Compared with high-protein yogurt prepared by a whey discharge process after fermentation, the concentrated high-protein yogurt prepared according to the present disclosure has similar or better soft texture, delicate taste, water holding capacity and digestibility, and is higher in calcium content.

Abstract: Disclosed is a transparent electrode made of a polymer matrix having semi-embedded therein a sintered silver nanowire composite comprising silver nanowires and metal oxide nanoparticles.

Abstract: The present disclosure provides a three-dimensional alkynyl-containing porous aromatic framework polymer and a preparation method and use thereof. The polymer has a structure represented by Formula I: The preparation method includes: under a protective atmosphere, mixing tetrakis(p-bromophenyl)methane, 1,3,5-triethynyl benzene, a catalyst and an amine solvent, and subjecting to a Sonogashira-Hagihara coupling reaction to obtain the three-dimensional alkynyl-containing porous aromatic framework polymer having the structure represented by Formula I.

Abstract: Provided herein are methods and reagents for treating cancer. Methods of treating cancer are provided, comprising administering to a patient in need thereof an effective amount of a DKK1 peptide-loaded MHC antibody provided herein. The methods can further include administering an effective amount of chemotherapy or immunotherapy to said patient.

Abstract: In some aspects, the disclosure relates to compositions and methods that are useful for treating inflammatory disorders and conditions. In some embodiments, the disclosure provides recombinant adeno-associated virus (rAAV) vectors and particles encoding a transgene that expresses a Myxomavirus serine proteinase inhibitor (Serp) protein (e.g., Serp-1) and methods for treating inflammatory disorders using the same.

Abstract: An electrode includes an electrically conductive substrate and a layer of a molybdenum-doped zinc/cobalt oxide (ZnCo2-xMoxO4). The surface of the electrically conductive substrate is at least partially covered by the layer of ZnCo2-xMoxO4, where x is a positive number equal to or less than about 0.1, and the layer of the ZnCo2-xMoxO4 includes spherical-shaped particles. The electrode has a Tafel slope from 75 millivolts per second (mV/s) to 115 mV/s, and a potential of 0.27 to 0.30 volts relative to the reversible hydrogen electrode (VRHE) at a current density of about 50 mA/cm2 for a duration of at least 40 hours.

Abstract: A first aspect of this work relates to water harvesting in power plants. A water adsorbent material is driven through adsorption-desorption cycles using waste heat from a power plant to harvest water from ambient air. In a preferred embodiment, metal-organic-framework (MOF) powders are used as the water adsorbent material for this application. A second aspect of this work relates more generally to rapid adsorption-desorption cycling of MOFs for various applications.

Abstract: A geopolymer composite including a porous aluminosilicate material, a quaternary ammonium surfactant, and a hydrophobicity modifier that includes dibenzoylmethane. The quaternary ammonium surfactant occupies sodium (Na) vacancies in the porous aluminosilicate material, and the hydrophobicity modifier is disposed on the quaternary ammonium surfactant.

Abstract: An antenna comprises a planar radiating structure, a ground plane and a feed structure. The radiation structure comprises a plurality of slots arranged symmetrically in concentric rings around an inner portion of the radiating structure. The slots are arranged to create a meandering current path on the radiating structure. The antenna produces an omnidirectional, monopole-like radiation field, and is relatively small with relatively high performance making it suitable for use in a wide variety of applications including those with challenging environments.

Abstract: An integrated structure according to a preferred embodiment may include a silicon or silicon-on-insulator (SOI) substrate, a conductive layer spaced apart from the substrate and including a metal or a metal compound, and a diffusion barrier layer provided therebetween. The substrate and the diffusion barrier layer may directly contact and form a van der Waals junction. Since the diffusion barrier layer may block diffusion of metal atoms into the substrate lattice by blocking movement of materials (atoms) between the substrate and the conductive layer and may also control injection of holes from the conductive layer toward the substrate, defects at the metal-semiconductor interface may be controlled to overcome the limitations of ultra-fine and highly integrated semiconductors.

Abstract: The present disclosure relates to a flame transfer function measurement system for prediction and reduction of combustion instability.

Abstract: A supercapacitor including a negative electrode, a positive electrode, an electrolyte, and a separator. The separator is between the negative electrode and the positive electrode, and the electrolyte is present in and on the separator. The negative electrode and the positive electrode include a carbon material selected from a poly-anthraquinone sulfide activated carbon material and a poly-anthraquinone sulfide reduced activated carbon material.

Abstract: The present disclosure discloses a defect location method and device based on coverage information, the method including: characterizing program structure information and test case coverage information of a target program in a graph to obtain a graph-characterized structure; generating a node attribute sequence and an adjacency matrix based on the graph-characterized structure; and inputting the node attribute sequence and the adjacency matrix to a trained graph neural network model, so that the graph neural network model outputs a suspicious degree list of the target program based on the node attribute sequence and the adjacency matrix. Through the graph-based unified information characterization, the coverage information can be saved without loss and compression, and the structure information may also be considered, thereby improving the accuracy of defect location.

Abstract: Disclosed, in part, are methods and compositions for evaluating two or more target proteins from the same family through the use of a molecular construct that contains a targeting group and a retrieval tag. The method can, in some aspects, quantify the activity and function of the proteins of interest, visualize active enzymes at the subcellular and intercellular scale, and increase dynamic range via signal amplification.

Abstract: ‘NCCH3’ is a new cultivar of flowering dogwood with a unique combination of traits including large white bracts, large glossy leaves, a fast growth rate, and showy fall color.