Abstract: Sequences of a serotype 8 adeno-associated virus and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles. rAAV8-mediated delivery of therapeutic and immunogenic genes, including Factor VIII, is also provided.
Type:
Grant
Filed:
September 6, 2007
Date of Patent:
September 7, 2010
Assignee:
The Trustees of the University of Pennsylvania
Inventors:
Guangping Gao, James M. Wilson, Mauricio R. Alvira
Abstract: In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.
Type:
Grant
Filed:
September 24, 2007
Date of Patent:
September 7, 2010
Assignees:
Agilent Technologies, Inc., Regents of the University of Colorado
Inventors:
Douglas J. Dellinger, Zoltan Timar, Joel Myerson, Geraldine Dellinger, Marvin H. Caruthers
Abstract: A bioreactor with substance injection capability. In one embodiment, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a chamber for receiving cells and a liquid medium. A port is formed in the second substrate between the bottom surface and the first surface of the second substrate. As formed, the port is in fluid communication with the chamber to allow a stream of substance to be introduced into the chamber. The stream of substance is controlled so as to provide a gradient, or a concentration gradient of the substance, to the chamber.
Type:
Grant
Filed:
August 27, 2003
Date of Patent:
September 7, 2010
Assignee:
Vanderbilt University
Inventors:
John P. Wikswo, Franz J. Baudenbacher, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Mark A. Stremler, Alissa Weaver
Abstract: An apparatus and method for cutting a material including conducting and non-conducting materials such as biological tissue, cellulose or plastic while the material is submerged in a conductive liquid medium. The apparatus has a cutting electrode with an elongate cutting portion having an aspect ratio (length to width) of 1 or more and a return electrode. The two electrodes are immersed in the conductive medium and a voltage is applied between them to heat the medium, thus producing a vapor cavity around the elongate cutting portion and ionizing a gas inside the vapor cavity to produce a plasma. The voltage applied between the electrodes is modulated in pulses having a modulation format selected to minimize the size of the vapor cavity, its rate of formation and heat diffusion into the material while the latter is cut with an edge of the elongate cutting portion. The modulation format includes pulses ranging in duration from 10 ?s to 10 ms, as well as minipulses and micropulses, as necessary.
Type:
Grant
Filed:
March 22, 2007
Date of Patent:
September 7, 2010
Assignee:
Board of Trustees of the Leland Stanford Junior University
Inventors:
Daniel V. Palanker, Alexander B. Vankov
Abstract: The present invention provides a dual-probe thermally compensated fluorescence decay rate temperature sensor capable of measuring the true temperature of a sample surface and its associated method of use.
Abstract: Disclosed are methods and systems for screening for compounds that act to modulate insect growth. Bioassays including cell culture and/or transgenic insects engineered with various components of the ecdysoid receptor (EcR) and/or the farsenoid-X receptor (RXR) systems to identify compounds that act as insecticides and/or hormone receptor activators are described. Also described are compounds, and compositions, identified as being putative insecticides based upon their ability to activate EcR and/or FXR mediated transcription.
Type:
Grant
Filed:
August 27, 2004
Date of Patent:
September 7, 2010
Assignee:
The University of North Carolina at Greensboro
Inventors:
Vincent C. Henrich, Cary Alan Weinberger
Abstract: A method for modifying the refractive index of an optical, polymeric material. The method comprises irradiating select regions of the optical, polymeric material with a focused, visible or near-IR laser having a pulse energy from 0.05 nJ to 1000 nJ. The irradiation results in the formation of refractive optical structures, characterized by a change in refractive index, exhibit little or no scattering loss, and exhibit no significant differences in the Raman spectrum with respect to the non-irradiated optical, polymeric material. The method can be used to modify the refractive index of an intraocular lens following the surgical implantation of the intraocular lens in a human eye.
Type:
Grant
Filed:
November 30, 2007
Date of Patent:
September 7, 2010
Assignees:
Bausch & Lomb Incorporated, University of Rochester
Inventors:
Wayne H. Knox, Li Ding, Jay F. Kunzler, Dharmendra Jani, Candido D. Pinto
Abstract: A method and system are provided for effecting interactive, three-dimensional renderings of selected body organs for purposes of medical observation and diagnosis. A series of CT images of the selected body organs are acquired. The series of CT images is stacked to form a three-dimensional volume file. To facilitate interactive three-dimensional rendering, the three-dimensional volume file may be subjected to an optional dataset reduction procedure to reduce pixel resolution and/or to divide the three-dimensional volume file into selected subvolumes. From a selected volume or subvolume, the image of a selected body organ is segmented or isolated. A wireframe model of the segmented organ image is then generated to enable interactive, three-dimensional rendering of the selected organ.
Abstract: A contour mode micromechanical piezoelectric resonator. The resonator has a bottom electrode; a top electrode; and a piezoelectric layer disposed between the bottom electrode and the top electrode. The piezoelectric resonator has a planar surface with a cantilevered periphery, dimensioned to undergo in-plane lateral displacement at the periphery. The resonator also includes means for applying an alternating electric field across the thickness of the piezoelectric resonator. The electric field is configured to cause the resonator to have a contour mode in-plane lateral displacement that is substantially in the plane of the planar surface of the resonator, wherein the fundamental frequency for the displacement of the piezoelectric resonator is set in part lithographically by the planar dimension of the bottom electrode, the top electrode or the piezoelectric layer.
Type:
Grant
Filed:
May 31, 2006
Date of Patent:
September 7, 2010
Assignee:
The Regents of the University of California
Inventors:
Gianluca Piazza, Philip J. Stephanou, Albert P. Pisano
Abstract: Assays are provided for the screening and classification of biologically active agents that alter the conformation of conformationally defective proteins. The methods of the invention find use in the identification and classification of agents with chaperone activity, particularly the identification and classification of small molecule chemical and pharmacological chaperones. The agents thus identified find use altering the conformation of otherwise conformationally defective proteins.
Type:
Grant
Filed:
January 26, 2007
Date of Patent:
September 7, 2010
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Abstract: Energy harvesting circuits and associated methods are provided that employ multiple antennas to optimize the amount of energy that is harvested while at the same time making efficient use of tag space. In some embodiments, matching networks are chosen in a manner that optimizes the DC energy that is created from the harvesting process. In other embodiments, phase shifts are introduced into the received signals to allow the signals to be more efficiently combined after they are rectified.
Type:
Grant
Filed:
April 2, 2009
Date of Patent:
September 7, 2010
Assignee:
University of Pittsburgh - Of the Commonwealth System of Higher Education
Inventors:
Marlin H. Mickle, Minhong Mi, Leonid Mats
Abstract: Provided is a hydrogen separation membrane characterized by comprising a structure obtained by sintering atomized powder having a composition of NixMyZr100-x-y (wherein M is Nb and/or Ta, 25?x?40, 25?y?40) and an average grain size of 50 ?m or less. The prepared hydrogen separation membrane does not require the use of costly Pd metal, and can be used as a substitute for conventional high-cost bulk metallic glass obtained by quenching of molten metal. This hydrogen separation membrane is free from problems such as defects in the hydrogen separation membrane and unevenness of composition, has a uniform structure, and is capable of separating hydrogen at low cost. Further provided are a sputtering target for forming such as hydrogen separation membrane and its manufacturing method.
Type:
Grant
Filed:
November 4, 2005
Date of Patent:
September 7, 2010
Assignees:
Nippon Mining & Metals Co., Ltd, Tohoku University
Abstract: Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising: a) a first portion of a G-protein coupled receptor (GPCR), where the first portion comprises the TM1, TM2, TM3, TM4 and TM5 regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, where the second portion comprises the TM6 and TM7 regions of the GPCR.
Type:
Grant
Filed:
October 15, 2008
Date of Patent:
September 7, 2010
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Abstract: A system, method, and computer-usable medium for removing redundancy from packet classifiers. In a preferred embodiment of the present invention, a packet classifier is implemented as a sequence of rules. A redundancy manager marks at least one upward redundant rule and at least one downward redundant rule. The redundancy manager removes at least one rule marked as upward redundant and at least one rule marked as downward redundant.
Type:
Grant
Filed:
May 31, 2006
Date of Patent:
September 7, 2010
Assignee:
The Board of Regents, University of Texas System
Abstract: The invention provides methods for in vitro maturation of immature human oocytes. In one aspect, the invention provides a method for in vitro maturation of immature human oocytes, comprising the steps of: (a) inducing in a female human subject an increase in endogenous luteinizing hormone levels, said subject having not undergone an ovarian stimulation protocol prior to said inducing step; (b) obtaining from said subject an immature oocyte; and (c) culturing said oocyte until maturity.
Abstract: Disclosed herein are a di-(4-vinylpyridine) metal phthalocyanine compound in which 4-vinylpyridines as ligands are included in a phthalocyanine having a metal, a composition comprising the same and a preparation method thereof. The di-(4-vinylpyridine) metal phthalocyanine compound has an excellent UV blocking effect together with low surface resistance and is prepared but by charging a powder phase with nitrogen, not by dissolving raw materials in an organic solvent as in the prior art.
Type:
Grant
Filed:
December 24, 2008
Date of Patent:
September 7, 2010
Assignee:
Hoseo University Academic Cooperation Foundation
Inventors:
Young Goo Kang, Dae Woo Ihm, Shi Surk Kim
Abstract: The present invention provides novel compositions containing a calbindin-D28k therapeutic element, which is involved in the regulation of apoptosis, and may be administered for the prevention of an abnormal apoptosis response in cells. In particular the compositions and methods of the present invention may be used for the prevention or induction of apoptosis in such cells types as osteoblasts and osteocytes. Specifically, the compositions and methods of the present invention are useful for the prevention of diseases associated with glucocorticoid induced cell death. Specifically, the compositions and methods of the present invention may be useful in the prevention of glucocorticoid induced cell death in osteoblasts and the treatment of such conditions as glucocorticoid induced osteoporosis.
Type:
Grant
Filed:
July 8, 2005
Date of Patent:
September 7, 2010
Assignee:
University of Medicine and Dentistry of New Jersey
Inventors:
Sylvia Christakos, Yan Liu, Teresita Bellido
Abstract: The present invention provides a recombinant HSV having the ability to specifically kill tumor cells such as human glioma cells in vivo, useful and safe for treating human glioma, etc. and that can easily put to clinical use. A new recombinant HSV, KeM345, is constructed by inserting a ?34.5 gene transcription unit, which is expressed by Musashi1 promoter, into a ribonucleotide reductase gene site of the genome of a herpes simplex virus (HSV) previously attenuated, by means of homologous recombination and obtained as a purified strain. Since KeM345 is a recombinant virus itself, it can not only induce viral proliferation by being transmitted to culture cells but can also induce viral replication equivalent to that of wild-type HSV, showing an excellent cytotoxic effect (cytolytic ability) selectively upon a malignant glioma.
Abstract: The invention provides a method for forming thin film transistors including a polycrystalline semiconducting film. The method comprises depositing a first layer of amorphous semiconducting thin film on to a substrate; depositing a second layer of thin film on to the first layer of amorphous semiconducting thin film; patterning the second layer of thin film so that the first layer of amorphous semiconducting thin film is exposed at selected locations; exposing the first and second layers of thin film to a nickel containing compound in either a solution or a vapor phase; removing the second layer of thin film; and annealing the first layer of amorphous semiconducting thin film at an elevated temperature so the first layer of amorphous semiconducting thin film converts into a polycrystalline semiconducting thin film.
Type:
Grant
Filed:
March 9, 2007
Date of Patent:
September 7, 2010
Assignee:
Hong Kong University of Science and Technology
Inventors:
Hoi Sing Kwok, Man Wong, Zhiguo Meng, Shuyun Zhao, Chunya Wu
Abstract: In the band edge region, transmission and reflection of an optical wave can be made very sensitive to the change in radiation wavelength, a change in the modulator material refractive index, and/or a change in the material absorption. Controlling these parameters with the increased level of sensitivity is provided by modulation using the band edge region. A preferred embodiment method of the invention uses a periodic optical structure (i.e., a grating) on top of an optical waveguide structure. The combination of the periodic structure and the optical waveguide is designed so that the reflection and/or transmission of the guided wave have broad pass bands with narrow transition bands. The optical structure is exposed to an incident laser radiation with wavelength in one of the transition bands. Modulation of the incident laser radiation is controlled by the change in refractive index or absorption in the optical guided wave structure produced by the modulation voltage.
Type:
Grant
Filed:
August 3, 2006
Date of Patent:
September 7, 2010
Assignee:
The Regents of the University of California