Abstract: To provide a novel method for obtaining a protein of interest efficiently in a soluble form when the protein is produced by expressing a foreign gene in host cells such as microorganisms etc. In a method for, by culturing host cells into which a foreign gene has been introduced, producing a protein encoded by the foreign gene, after induction of expression, recombinant cells are cultured in the temperature range lower than or equal to the upper temperature limit for growth of the host cells and higher than 5 degrees below the upper temperature limit for growth of the host cells.

Abstract: The present invention provides an agent containing L-tyrosine that prevents methemoglobin formation, and a vesicle comprising the above agent for preventing methemoglobin formation. More specifically, the present invention provides an oxygen infusion preparation suitable for long-term storage, which prevents an increase in methemoglobin content as a result of oxidation of hemoglobin or the like encapsulated in an vesicle having a lipid bilayer membrane structure.

Abstract: A micro system capable of setting an appropriate amount of stimulation being applied in order to control liquid flow in a channel. The micro system comprises micro-heaters (5b, 5c) for applying stimulation to liquid flowing through liquid channels (2b, 2c) formed in a plate (1) and controlling liquid flow by the stimulation from the micro-heaters (5b, 5c), and a means for electrically controlling the amount of stimulation being applied to the liquid from the micro-heaters (5b, 5c). An appropriate amount of stimulation can be set by electrically controlling the amount of stimulation being applied to the liquid from the micro-heaters (5b, 5c) through the control means.

Abstract: A magnetic recording medium comprises a substrate, an undercoat layer, a magnetic layer, and a protective film. The undercoat layer and the magnetic layer are provided atop the substrate. The magnetic layer contains a noble metal layer containing Pt or Pd, a cobalt layer containing Co, and a non-magnetic material layer containing C or Si or Ge.

Abstract: A method is provided for recurrently estimating a spectrum of noise at each signal observation interval from a sound signal which contains the noise and which is observed at each signal observation interval. In the method, there are acquired an envelope of a previous spectrum of the noise which has been previously estimated from the sound signal observed at a previous signal observation interval, and an envelope of a current spectrum of the sound signal which is observed at a current signal observation interval subsequent to the previous signal observation interval. Then, a value of correlation is computed between the envelop of the previous spectrum of the noise and the envelope of the current spectrum of the sound signal. A current spectrum of the noise contained in the sound signal observed at the current signal observation interval is estimated in accordance with the computed value of the correlation and based on the previous spectrum of the noise and the current spectrum of the sound signal.

Abstract: A method is designed for emphasizing a howling frequency component of a sound signal observed in an acoustic feedback system during an observation period having a predetermined length.

Abstract: A main object of the present invention is to provide a charge-giving body capable of forming a pattern having a minute structure on a surface of a semiconductor by a simple process; and a minute pattern-formed body. The present invention is a charge-giving body, comprising a defected region in which defect is introduced into a crystal structure in a crystalline semiconductor surface, charge being given from the defected region.

Abstract: The present invention provides a novel detecting reagent for double-stranded nucleic acid, and a method of using it to detect double-stranded nucleic acid formed by hybridization with a probe, with absolutely no labeling of the target nucleic acid. The detecting reagent for double-stranded nucleic acid of the invention is characterized by comprising, in the same molecule, a naphthalenediimide group which is intercalatable into double-stranded nucleic acid and a &bgr;-diketone group capable of forming a lanthanoid metal complex.

Abstract: An apparatus for measuring a glucose concentration is noninvasive, accurate, and compact. The apparatus includes a detection unit (10) and a principal component analysis (PCA) unit (20). The detection unit includes a light source (11) to emit mid-infrared light, an ATR prism (12) to receive the mid-infrared light with a measuring object placed on the ATR prism, and a spectrum detector (13) to detect an absorption spectrum from the mid-infrared light from the ATR prism. The PCA unit includes a loading memory (24) to store a glucose corresponding loading and a personal data memory (28) to store personal data in the form of a calibration curve. According to the detected absorption spectrum, stored loading, and stored personal data, the PCA unit computes a glucose concentration of the measuring object.

Abstract: A grating formation method comprises the steps of accelerating ions; causing the accelerated ions to pass through a mask having a shape corresponding to the grating; and implanting the ions, which have passed through the mask, into the core, to form refractive index-variation portions for the grating in the core. The other formation method comprises the steps of accelerating ions, converging an ion beam formed of the accelerated ions, to make a beam diameter identical with or smaller than a width of refractive index-variation portions in a direction of the central axis of the optical waveguide so as to provide a converged ion beam; and intermittently irradiating the converged ion beam to the core, while moving an irradiation position in the direction of the central axis, to implant the ions into the core so as to form the refractive index-variation portions in the core.

Abstract: The present invention provides a novel detecting reagent for double-stranded nucleic acid, and a method of using it to detect double-stranded nucleic acid formed by hybridization with a probe, with absolutely no labeling of the target nucleic acid.

Abstract: A soft magnetic thin film in the form of a Co—Fe—Ni—C thin film consisting essentially of 40-70% by weight of cobalt, 20-40% by weight of iron, 5-20% by weight of nickel, and 0.02-0.1% by weight of carbon has a high saturation magnetic flux density, excellent soft magnetic properties, and a high electric resistivity.

Abstract: A magnetic recording medium comprising a substrate 1 having thereon an in-plane magnetic film 3 having a magnetic easy axis oriented in the in-plane direction with respect to the substrate and a vertical magnetic film 6 having a magnetic easy axis oriented perpendicularly to the substrate. The in-plane magnetic film 3 is disposed closer to the substrate 1 than the vertical magnetic film 6. In preferred embodiments, the magnetic recording medium may further comprise a separation film 4 disposed between the in-plane magnetic film 3 and the vertical magnetic film 6, a nonmagnetic intermediate layer 5 having an hcp structure disposed between the separation film 4 and the vertical magnetic film 6, and a protective film 7. The magnetic recording medium is adapted for use with a signal processing system generally used for in-plane magnetic recording media without modification of the signal processing system.

Abstract: A soft magnetic thin film for magnetic head has high saturation magnetization and at the same time high resistivity. There are provided a method of manufacturing the soft magnetic thin film and a magnetic head and a magnetic storage unit which use the soft magnetic thin film. At least one of lower and upper soft magnetic layers of magnetic head which define a magnetic circuit is formed of a soft magnetic thin film having as a main constituent a Co--Ni--Fe alloy consisting of 30 to 90 at % Co, less than 40 at % Ni and less than 40 at % Fe and containing at least S by 0.5 to 4 at %. The soft magnetic thin film is formed from a Co--Ni--Fe alloy plating solution containing metal salts of Co, Ni and Fe and additionally containing a sulfur system organic compound through electrodeposition process.

Abstract: A method for mechanically removing roots from harvested cormatose plant bodies using a special root removing arrangement comprises supplying the harvested cormatose plant body (3) in between two parallel spaced horizontal rollers (1,2) while they are rotating in opposite directions relative to each other so that their peripheral or opposing adjacent inner sides are moving upwards, or in the same rotational direction so that one of these peripheral surfaces on opposing adjacent inner sides is moving upwards, in order that the plant bodies roll on the rollers and become balanced and oriented substantially axially of the rollers, changing the rotational mode of the rollers into a counter rotation mode so that their peripheral surfaces on the opposing inner sides are moving downwards, drawing the beard-like roots (4) of each plant body (3) into the gap between the rollers, forcing each plant body away from the rollers by a plant body thrusting mechanism (5,6,7) to cause the roots to be plucked from the plant body

Abstract: Disclosed herein is an instrument for inoculating bulb scales which comprises an instrument line, a scale sucking finger having a suction tube, and a scale gripping finger, with one end thereof rotatably joined to the instrument proper and with an intermediate part thereof connected to the instrument proper with a spring made of shape memory alloy.

Abstract: A three phase fluidized bed water purifying process comprises purifying effluents in a three phase fluidized bed composed of solid particles, an effluent, and a gas in fluidized state, the solid particles being made by mixing a binder with an inorganic material based on aluminium silicate, granulating the resulting mixture, and firing the granules to sinter them. The present invention permits the purification of effluent for a long period of time without oxygen deficiency.