Abstract: Methods and compositions are provided for improved protein binding assays by preparing compositions having indirectly labeled ligands substantially free of label conjugated to materials other than the indirectly labeled ligand. The method for preparing the compositions involves employing a monovalent receptor to which the label is conjugated and combining the monovalent receptor labeled conjugate to the ligand, either in pure or impure form. The mixture is then segregated according to molecular weight and the ligand conjugated to the labeled receptor isolated. This conjugate may then be directly used as a reagent in a protein binding assay, where the assay mixture is substantially free of label other than labeled receptor bound to ligand.The labels will be for the most part of relatively low molecular weight, while the receptor is preferably a Fab fragment.

Abstract: Compositions comprising HB.sub.s Ag and a physiologically acceptable medium are useful as diagnostic and immunologic agents.

Abstract: This invention relates to a method of production of formaldehyde, and lower saturated aliphatic dialdehyde-treated allergens suitable for the immunotherapy (desensitization) of individuals suffering from allergies of the immediate type in a plurality of steps wherein said formaldehyde and/or said dialdehyde is utilized alone or in combination for the modification of allergen-containing materials. These lower saturated aliphatic mono- and dialdehyde treated allergen-containing materials are useful in ameliorating the symptoms of allergic (atopic) people. Also, they are able to induce formation of blocking antibody in mammals which cross-reacts with the native allergen-containing materials. Reactions with formaldehyde are carried out in a non-phenolic environment in a plurality of steps. The reaction involving combinations of aldehydes can be carried out in a single step.

Abstract: A method of detecting antibodies to native deoxyribonucleic acid (n-DNA), anti-nuclear antibodies, antibodies to non-histone containing protein, rheumatoid factors, antibodies to thyroglobulin, and an immunofluorescent assay for such detection is described.

Abstract: A method for the estimation of an immune complex and analysis of a constituent thereof comprising a latex particle agglutination test, in which a sample containing immune complex is incubated with latex particles coated with low affinity non-human antibodies to said complex constituent.

Abstract: A synthetic physiological mucus having particular usefulness as a vaginal and surgical lubricant, based upon a pituitous aqueous solution of a high molecular weight polyacrylamide.

Abstract: Three antigens, termed HA, MN and FN, are prepared for the serological diagnosis of infectious mononucleosis; the HA antigen being used to identify the heterophil antibodies and the FN and MN antigens are used to differentiate them.

Abstract: The invention relates to the testing of humans or other animals, such as horses, cattle or dogs, for allergic reaction or hypersensitivity to penicillins. The tests can be used both to predict and to diagnose allergy. The invention comprises new penicilloyl-polylysine (PPL) preparations, new materials for use in minor determinant mixture (MDM) compositions and novel test methods employing such materials.The new PPL preparations comprise homogeneous, high purity, maximally coupled, .alpha.-diastereoisomeric, penicilloyl conjugates of low molecular weight PPLs. The PPL materials of the invention have the molecular structure in accordance with the following generic formula: ##STR1## wherein: R is selected from the group consisting of H and penicilloyl groups or similar groups derived from cephaolosporins or other .beta.-lactam antibiotics; andn is an integer of from 4 to 10,at least about 66% and up to 100% of the R groups are other than hydrogen.

Abstract: Latex reagents for quantitative assay of a variety of steroid hormones or metabolites thereof which are contained in body fluid or excreted fluid of human beings comprise latex particles which are immunologically sensitized with a conjugate selected from a variety of steroid-serum albumin conjugates in which the steroid to be detected is bonded to serum albumin in a very small ratio ranging from 0.5 to 7 molecules per 1 molecule of the serum albumin used.Sensitization of latex particles with such a conjugate is performed using a limited amount dependent on the bonding ratio of steroid molecules per molecule of serum albumin in the conjugate and the particle size of the latex used.The latex reagents show very high sensitivity amounting to 0.2-0.04 nmole steroid equivalent/ml.

Abstract: Disclosure relates to novel hydrolytic enzyme product derived from the pineapple plant useful for the digestion, dissection and separation of non-viable, devitalized tissue, especially eschar tissue from viable tissue in a mammalian host.

Abstract: This invention relates to the production of formaldehyde treated allergens suitable for the immunotherapy (desensitization) of individuals suffering from allergies of the immediate type. These formalinized allergen-containing materials are also useful to induce formation of antibody in mammals which cross reacts with the native allergen-containing materials.

Abstract: The present invention encompasses a method for enumerating microbial cells in an aqueous test sample comprising: fluorescently labeling the microbial cells in test sample intermixing a known amount of monodispersed fluorescently labeled beads of about cell size into the test sample, and determining the ratio of bacterial cells to fluorescently labeled beads, the reagents for practicing this method. The methods and reagents of the present invention are particularly useful in counting bacterial cells in urine.

Abstract: Three antigens, termed HA, MN and FN, are prepared for the serological diagnosis of infectious mononucleosis; the HA antigen being used to identify the heterophil antibodies and the FN and MN antigens are used to differentiate them.

Abstract: 1-substituted-4-aryl-quinazolin-2(1H)-ones and thiones and pyrido[2,3-d]pyrimidin-2-ones, eg. 1-isopropyl-7-methyl-4-phenyl-quinazolin-2(1H)-one, are useful as platelet aggregation inhibitors.

Abstract: Three antigens, termed HA, MN and FN, are prepared for the serological diagnosis of infectious mononucleosis; the HA antigen being used to identify the heterophil antibodies and the FN and MN antigens are used to differentiate them.

Abstract: Process for identification and enumeration of B and T lymphocytes in an unseparated peripheral body sample comprises contacting the sample with two morphologically distinguishable bacteria, each of which is coated with an antibody specific to an antigen on one of the B or T cells. The presence of a B or a T cell is determined by rosette formation with the corresponding bacteria, as viewed under a microscope in a smear.

Abstract: Prolonged release pharmaceutical preparations containing ion exchange resin drug complexes at least a substantial portion of which have been treated with a solvating agent and provided with a diffusion barrier coating.

Abstract: Compositions useful for platelet antiaggregation activity are described, which contain as the active ingredient N-(2-chlorobenzyl)-pyrrolidine or a pharmacologically compatible salt thereof with an organic or an inorganic acid, and at least one pharmaceutically acceptable carrier or excipient. The compositions in the form of an aqueous solution are administered orally in the dose of 2-8 mgs/kg, preferably 3 mgs/kg body weight twice a day. The compositions may also be administered intravenously or endoperitoneally. The compositions may be administered in the form of capsules, ampoules or in the form of oily suspensions, solutions or emulsions.

Abstract: A method for preparing an antigen comprising a peptide-protein complex which comprises reacting a peptide represented by the general formula (I) ##STR1## wherein m is 0 or 1, as a hapten, with a protein as a carrier in the presence of a dialdehyde represented by the general formula (II)OCH--(CH.sub.2).sub.n --CHO (II)wherein n is an integer of 1 to 5 and a method for preparing an antibody specific to pancreatic glucagon comprising administering the above described antigen to a mammal and recovering the antibody produced.

Abstract: 1-Substituted pyrazole-5-ones of the formula ##STR1## and pharmaceutically acceptable, nontoxic salts thereof, wherein R is hydrogen or amino;R.sup.1 is hydrogen, alkyl, alkenyl, unsubstituted or substituted aryl or unsubstituted or substituted aralky;X isa. ethylene, ethylene wherein 1 hydrogen atom on one of the carbon atoms is substituted by alkyl of 1 to 4 carbon atoms, ethylene wherein 1 hydrogen atom on each of the two carbon atoms is substituted by alkyl of 1 to 4 carbon atoms or said ethylene which is linked to R.sup.2 via an oxygen or sulphur atom;b. propenyl, propenyl wherein 1 hydrogen atom on one, two or three of the carbon atoms is substituted by alkyl of 1 to 4 carbon atoms, said propenyl being linked to the N.sup.1 atom of the pyrazol ring via its methylene moiety; orc. methylene;provided that when X is methylene, R.sup.1 is not hydrogen if R is amino;R.sup.2 is aryl unsubstituted or substituted by:a.