Abstract: A product, Malignin, derived from brain tumor cells, which cells may be cultured in a suitable nutrient medium for many generations, and the method of preparing same. Malignin may be complexed with an inert carrier, such as bromoacetylcellulose, and employed in tests for brain tumors. The antibody to Malignin is also prepared. A complex of Malignin and an inert carrier may be further complexed with Anti-Malignin.

Abstract: Purified soluble antigen, specific for rubella virus, is isolated from growth media of rubella-infected cell cultures by affinity and gel permeation chromatography and characterized, inter alia, by its specific activity. Antigen-sensitized particles are employed as immunoassay reagents in, for example, agglutination assays for detection and quantification of rubella antibodies in body fluids such as serum, spinal fluid and the like.

Abstract: A process for coupling biological substances by covalent bonds that involves the use of benzoquinone as cross-linking agent in large excess compared with the substance to be activated. The activation reaction is realized in a homogeneous liquid medium. The process makes it possible, for example, to couple antibodies to enzymes for determining or detecting said antibodies.The process can be used for the determination of antitetanic antibodies in human serum.

Abstract: The subject invention concerns novel compositions for use in immunoassays, as well as immunoassays employing such novel compositions. The compositions comprise discrete charged colloidal particles comprised of small molecules which particles are capable of retaining their discrete character in an aqueous medium and composed of aggregates of lipophilic and/or amphiphilic organic molecules to which are bound non-covalently a label capable of producing a detectible signal and a ligand or an analog of the ligand capable of competing with a ligand for a ligand receptor. The discrete colloidal particle serves as a hub or nucleus for retaining the ligand or its analog and the label within a limited locus.The compositions are prepared by individually covalently bonding the ligand and the label, when not naturally lipophilic, to a lipophilic (includes amphiphilic) compound, normally a phospholipid.

Abstract: A process for quantitative determination of the amount of antigen in a solution while using hemagglutination inhibition technique, comprising preparing under serial dilution a series of samples of said solution, adding to each of said samples a predetermined amount of antiserum containing antibodies specific with regard to the antigen in question, adding after incubation to each of the resulting samples a predetermined amount of the antigen carried by a particulate carrier resulting in hemagglutination, and preparing a corresponding series of control samples with known decreasing amounts of antigen. By measuring the diameter of the hemagglutination deposits of the control samples and plotting the measured diameters against the antigen concentrations, an S-shaped curve is obtained, the middle part of which has a steep inclination.

Abstract: In a mixture of two antibody-coated latex particle suspensions, the particles in each suspension being of a different size, respectively, absence of antigen specific to the antibody in the mixture leaves multiplets only of one and the other sizes of particles. Presence of antigen in the mixture results in large particle/small particle coupling, which is detected by the resistive pulse method and indicates that an antigen-antibody reaction has occurred.

Abstract: Therapeutic antihypertensive compositions containing 2-[1-(2,6-dichlorophenoxy)ethyl]-4,5-dihydro-1H-imidazole and N-(2-chloroethyl)-N-(1-methyl-2-phenoxyethyl)benzenemethanamine or their non-toxic, pharmaceutically acceptable salts are described as well as a method of reducing blood pressure in mammals.

Abstract: A method of differential diagnostic sperm examination and the use of prestained slides in that method comprising placing the specimen to be examined on a prestained blood-picture slide, developing the stain and examining same thereafter.

Abstract: Emollient-containing urea bath bead compositions are disclosed in which an oily perfume is present in a low-foaming or nonfoaming system, while minimizing scum formation on the bathtub walls. Also, anticaking particles are associated with the prills in a nonsegregating fashion, and water is included in the materials sprayed on the urea prills so that water soluble dyes can be dissolved in the water and applied to the prills at the same time.

Abstract: Aryloxyalkylaminobenzoic acids and esters as hypolipemic compounds.

Abstract: A method of reducing the lipid concentration in the blood of a patient comprises administering to the patient a lipid-lowering effective amount of silica gel.

Abstract: A method of inhibiting the complement system in a warm-blooded animal which comprises the administration of a ureylenebis-[substituted-phenylenecarbonyl (and sulfonyl) imino-substituted-phenylene carbonylimino-naphthalenetrisulfonic acid, hexaalkali metal salt].

Abstract: A diagnostic reagent for detecting the presence of specific microbial antibodies in body fluids which comprises a gel containing intact erythrocytes, which have appropriate microbial particles adhering directly or indirectly to the surface thereof, the gel being contained in a shallow vessel. The system may additionally include complement, or alternatively this may be added separately and after test-body fluids have been applied to the gel. Interaction of specific antibodies with the microbial particles, in the presence of complement results in the lysis of the erythrocytes thereby forming a visible positive result.

Abstract: Hypolipidemic agents of the formula: ##STR1## wherein X is C.dbd.O or CHOH,R.degree. is branched lower alkyl,R is hydrogen, alkyl, or halo,n is 1 or 2, andR' and R" are hydrogen, halo, alkyl or alkoxy, e.g., 4-phenoxy pivalophenone.

Abstract: Immunoassays are provided employing antibodies and a fluorescer-quencher (F-Q) chromophoric pair, wherein one or both of the chromophoric pair are bonded to antibodies. Depending on the particular ligand of interest, various reagent combinations can be employed, where the amount of quenching is directly related to the amount of ligand present in the assay medium.In carrying out the assay, the unknown and antibody specific for the ligand of interest to which is bound one of the F-Q pair, are combined in an aqueous buffered medium. Depending on the protocol, different assay reagents are employed in the aqueous buffered medium: (1) ligand analog bonded to the other of the F-Q pair; (2) antibodies specific for the ligand to which is bound the other of the F-Q pair or; finally, (3) a combination of a plurality of ligands bonded together through linking groups to a hub molecule, usually a polymer, in combination with antibody bound to the other of the F-Q pair.

Abstract: Aerosol antiperspirant compositions are provided that are highly concentrated with respect to the active astringent salt, and capable of being dispensed from aerosol containers of the foam type at a low delivery rate, comprising, in combination, an astringent salt in an amount within the range from about 8 to about 30%; a liquefied propellant, or a mixture of liquefied propellants, each with a vapor pressure at 21.degree. C. of at least 2.4 atmospheres absolute, having a low molecular weight, in an amount of at least 0.15 mole per atmosphere absolute pressure in the container at 21.degree. C. per 100 g of composition; a nonvolatile miscible organic liquid in an amount within the range from about 0.1 to 30% by weight of the composition; a bulking agent in an amount within the range from about 0.1 to about 5% by weight of the composition; and a solid aliphatic carboxylic acid having from about fourteen to about twenty-two carbon atoms in a straight chain and in an amount within the range from about 0.

Abstract: A solid pharmaceutical composition comprising anhydrous cholestyramine, 0.6 to 1.7 parts by weight of low viscosity grade alginic acid/sodium alginate per weight of cholestyramine, the low viscosity grade alginic acid/sodium alginate having 0 to 75% of the acid groups neutralized, 0.1 to 0.3 parts by weight of citric acid per weight of sodium alginate, and sufficient sodium carbonate or bicarbonate mixtures thereof to neutralize the acid groups of the alginic and citric acids. The compositions may be used in the treatment of conditions associated with duodeno-gastric reflux of bile into the stomach.

Abstract: A series of novel 4-amino-2-(thiadiazole-carbonyl piperazinyl)-6,7-dimethoxyquinazolines is disclosed having antihypertensive properties. The thiadiazole substituent may be optionally substituted with alkyl or lower alkoxy carbonylamino groups. A representative embodiment of the invention is 4-amino-6,7-dimethoxy-2-[4-(5-ethoxy-carbonylamino-1,2,3-thiadiazole-4-car bonyl)-piperazin-1-yl]-quinazoline.

Abstract: A quantitative evaluation of the intestinal microflora is made by administering to an animal organism an effective amount of novel chemical conjugates of a bile acid or related acid with an amino acid which will be cleaved from the bile or related acid by the enzymes elaborated by the intestinal microflora and then measuring the amount of the released amino acid in the urine after a predetermined time interval. The novel compounds and their use in in vitro tests are included in the invention.

Abstract: An animal feed containing 0.00002 to 0.1% by weight of:2-methyl-7-methoxy-4'-nitroisoflavone,7-[hydroxyethoxy]-2-methylisoflavone,7-carbethoxy-2-methylisoflavone,7-ethoxyisoflavone,7-n-benzyloxy-2-methylisoflavone,7-p-nitrobenzyloxyisoflavone,7-p-nitrobenzyloxy-2-methylisoflavone,7-p-chlorobenzyloxyisoflavone,7-cetyloxy-2-methylisoflavone,7-(2-octyloxy)-isoflavone,7-benzyloxy-3',4'-dimethoxyisoflavone, and7-hexadecyloxyisoflavoneAs a weight-gain promoting anabolic ingredient.