Abstract: Disclosed herein are methods and compositions for determining the presence or absence of a mechanism of antimicrobial resistance in a sample.

Abstract: Glomus iranicum var. tenuihypharum var. nov. strain deposited under BCCM deposit number 54871, comprising the sequence identified by SEQ ID NO: 1; composition having the strain and 2:1 smectite clays and use thereof as bio-stimulant. The invention also discloses a composition having the strain, fungicides, bio-fungicides, insecticides, bio-insecticides, nematicides and bio-nematicides.

Abstract: One aspect of the present disclosure relates to a device for detecting a target analyte in a liquid sample. The device can comprise a housing. The housing can include an inlet for receiving a liquid sample, an outlet for removing a volume of the liquid sample from the device, a filter associated with the outlet and being sized and dimensioned to retain a target analyte on a surface thereof, and a flow system comprising at least one channel that is in communication with the inlet and the outlet. At least a portion of the at least one channel can be located substantially adjacent the surface of the filter and be shaped and dimensioned to reduce the amount of unreacted fluorescent probe available to create the background interference during detection of the target analyte.

Abstract: A detection system for determining pyruvate dehydrogenase (PDH) levels in a bodily sample includes at least one reaction solution for generating NAD+ upon combination with PDH in the bodily sample, the reaction solution including pyruvate and NADH and a biosensor for determining the level of generated NAD+.

Abstract: A cell culture medium comprising adenosine triphosphate; a carrier protein; cholesterol, linoleic acid, and lipoic acid; glutathione; at least one nucleotide salvage pathway precursor base; phosphoethanolamine; selenium; transferrin; triiodothyronine; all-trans-retinoic acid (ATRA) and vitamin C; zinc, magnesium, and copper; an agent that increases intracellular cAMP; epidermal growth factor (EGF); hydrocortisone; insulin; and charcoal stripped fetal bovine serum, wherein said cell culture medium is substantially free, if not entirely free, of vitamin D, androgenic hormones, androgenic ligands, estrogenic hormones, estrogenic ligands, and/or androgenic receptors.

Abstract: Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer.

Abstract: Disclosed are compositions and methods related to eukaryotic microorganisms that can produce unsaturated fatty acids which can be purified and used.

Abstract: This invention relates to enrichment of a biological sample comprising a plurality of cells to assist further analysis thereof. It provides a technique comprising the steps of: (a) providing a sample comprising a plurality of cells which include a photosensitive compound that can be induced by light irradiation to inactivate or kill at least part of the respective cell; (b) acquiring an image of at least a portion of the sample; (c) identifying cells of interest in the sample image; (d) selecting cells other than the cells identified in step (c); and (e) irradiating only those cells selected in step (d) with a light beam so as induce the photosensitive compound therein to inactivate or kill at least part of those cells, and thereby enrich the sample with respect to the cells of interest for further analysis.

Abstract: Methods for detecting biomarkers of inflammation, infection, and/or bacterial activity in dairy production, which indicate issues with the milk itself or issues related to the health of the cow. The methods generally comprise contacting a milk sample with a nanoplatform assembly to create an assay solution, and detecting spectral changes in the assay solution that are triggered by enzymatic activity (when present) in the sample. The nanoplatform assembly comprises a first particle, a second particle, and a linkage therebetween, wherein the linkage comprises a protease consensus sequence (the sequence of amino acids cleaved by the protease), or an ester linkage (cleaved by a protease or lipase). A plurality of second particles can also be linked to the first particle. Test strips are also described, which undergo a visual color change in the presence of the target enzyme in the milk sample.

Abstract: Disclosed is a method for reducing measurement errors due to inhibition of catalase by azide in a method for quantification of a component to be measured, in which hydrogen peroxide derived from a component other than the component to be measured is decomposed by a catalase. The method for reducing measurement errors due to inhibition of catalase by azide employs a catalase which has a subunit having a molecular mass of 75 kDa or higher and is derived from a microorganism, when hydrogen peroxide derived from a component other than the component to be measured is decomposed by the catalase followed by quantification of hydrogen peroxide derived from the component to be measured to quantify the component to be measured.

Abstract: The present invention relates to cell-based methods for determining the biological activity of defibrotide. In particular, the invention provides a method for assessing the potency of defibrotide by assessing the viability of mammalian cells in the presence of at least one cytotoxic agent and one or more concentrations of defibrotide. Such methods are particularly useful for standardizing pharmaceutical compositions comprising defibrotide.

Abstract: Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed.

Abstract: A dry animal-derived collagen fiber tissue material and preparation method and bioprosthesis thereof are disclosed. The preparation method includes: 1) rinsing of an animal-derived collagen fiber tissue material that has been treated with a crosslinking agent; 2) immersion of the rinsed tissue material in a non-aqueous alcoholic solution for dehydration; 3) successive immersion of the tissue material that has been dehydrated with the non-aqueous alcoholic solution in saccharide solutions of different gradients of concentrations for gradient dehydration; 4) taking out and drying of the gradient dehydrated tissue material; and 5) hermetic packaging of the dried tissue material and sterilization. The preparation method is simple and allows the use of easily-available low-cost materials, resulting in lower costs. In addition, it can reduce the toxicity caused by a residue of the aldehyde crosslinking agent.

Abstract: A device for single-cell analysis according to an embodiment of the present invention comprises: a substrate; a gap between the substrate and porous membrane which is a space for culture medium; and a porous membrane formed on having a pore capable of isolating a second cell into single cell units. A method for single-cell analysis according to an embodiment of the present invention comprises: Culturing a first cell in a culture medium on a bottom side of porous membrane; Applying a sample including a second cell on a porous membrane in a culture medium; Isolating the second cell into single cell units in a pore existing in the porous membrane with a external force such as agitation and gravitational force; Generating an interaction situation between the first cells and the single cell-level second cell; Analyzing a cellular phenomena of the first cell or the second cell.

Abstract: An in-vivo intravascular blood replacing liquid of the present invention is injected into a blood vessel to replace blood at an intravascular portion to be inspected therewith in making an in-vivo intravascular inspection. The blood replacing liquid comprises an aqueous medium unharmful for a living body and a gelling property imparting substance, unharmful for the living body, which is added to the aqueous medium to impart a gelling property to the blood replacing liquid. The blood replacing liquid has a viscosity of not more than 3 mPa·s when the blood replacing liquid is injected into the blood vessel.

Abstract: Processes are disclosed for economically and effectively removing co-produced oxygenated organic compound from an anaerobic, aqueous fermentation broth used for the bioconversion of syngas to product oxygenated organic compound. The processes involve subjecting a portion of the aqueous fermentation broth after recovery of the product oxygenated organic compound to anaerobic organic bioconversion, and recycling the broth for use in the bioconversion of syngas.

Abstract: To provide an effective 1,4-dioxane-degrading bacteria culture method. Provided is a 1,4-dioxane-degrading bacteria culture method in which 1,4-dioxane-degrading bacteria are propagated using a medium containing diethylene glycol.

Abstract: A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery, a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.

Abstract: Disclosed herein is an improved method for magnetic capture of target molecules (e.g., microbes) in a fluid. Kits and solid substrates for carrying the method described herein are also provided. In some embodiments, the methods, kits, and solid substrates described herein are optimized for separation and/or detection of microbes and microbe-associated molecular pattern (MAMP) (including, e.g., but not limited to, a cell component of microbes, lipopolysaccharides (LPS), and/or endotoxin).

Abstract: Provided are a method for preparing a liquid-state dripping or coating pathological quality control product, and uses thereof. The method comprises: selecting and determining a control with a control value, and processing the control; adding an ethanol solution to the processed control for preserving for standby use, with the amount of the ethanol solution added depending on the amount of a precipitate; and performing setting of a positive or negative control by means of dripping or smearing. The pathological quality control product is a suspension or homogenate of micro tissue sections, cell/cultured cell sections, or cultured cells. Another aspect of the present invention provides a use of the liquid-state dripping or coating pathological quality control product as a positive or negative control in immunohistochemistry, in situ hybridization, special staining and other tissue staining detection, or as a standard quality control product for pathological internal quality control and external quality control.