Patents Examined by Bin Shen
  • Microparticles having reference markers arranged in different concentrations
    Patent number: 10168271
    Abstract: Methods for sorting cells include: arranging microparticles into an array on a substrate in a microfluidic device, in which the microparticles each include multiple reference markers; introducing multiple cells to the array of microparticles under conditions that enable at least some of the cells to adhere to the microparticles; removing the microparticles, to which the cells are adhered, from the substrate; transferring the microparticles, to which the cells are adhered, to a detection region; and detecting, for each of two or more microparticles that pass through the detection region, a microparticle feature; and sorting the two or more microparticles based on the detected features, in which the detected features are related to a phenotype of the cells.
    Type: Grant
    Filed: November 3, 2014
    Date of Patent: January 1, 2019
    Assignee: The General Hospital Corporation
    Inventors: Daniel Irimia, Ki Wan Bong
  • Chemoenzymatic synthesis of heparin and heparan sulfate analogs
    Patent number: 10160986
    Abstract: The present invention provides a one-pot multi-enzyme method for preparing UDP-sugars from simple sugar starting materials. The invention also provides a one-pot multi-enzyme method for preparing oligosaccharides from simple sugar starting materials.
    Type: Grant
    Filed: February 5, 2016
    Date of Patent: December 25, 2018
    Assignee: The Regents of the University of California
    Inventors: Xi Chen, Hai Yu, Yanhong Li, Yi Chen, Jingyao Qu, Musleh M. Muthana, Kam Lau, Lars Bode
  • Methods for treating obesity and/or metabolic syndrome
    Patent number: 10159696
    Abstract: The present disclosure provides methods of treating or preventing obesity or causing weight loss or treating or preventing metabolic syndrome comprising administering to a subject a population of cells enriched for STRO-1+ cells and/or progeny thereof and/or soluble factors derived therefrom.
    Type: Grant
    Filed: June 8, 2016
    Date of Patent: December 25, 2018
    Assignee: MESOBLAST, INC.
    Inventors: Ravi Krishnan, Silviu Itescu
  • Devices and formulations for detecting, screening and monitoring levels of certain constituents in bodily fluids and method
    Patent number: 10160994
    Abstract: A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-laden carrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.
    Type: Grant
    Filed: March 20, 2018
    Date of Patent: December 25, 2018
    Assignee: Pop Test LLC
    Inventors: Randice Lisa Altschul, Neil Theise, Myron Rapkin, Rebecca O'Brien
  • Alpha-galactosidase A and 1-deoxygalactonojirimycin co-formulation for the treatment of fabry disease
    Patent number: 10155027
    Abstract: The present application provides compositions comprising ?-galactosidase A in combination with an active site-specific chaperone for the ?-galactosidase A, and methods for treating Fabry disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an ?-galactosidase A enzyme formulation. The present application also provides methods for treating Fabry disease using intravenous administration of 1-deoxygalactonojirimycin.
    Type: Grant
    Filed: February 7, 2017
    Date of Patent: December 18, 2018
    Assignee: Amicus Therapeutics, Inc.
    Inventors: Richie Khanna, Kenneth Valenzano, Susan Elizabeth Fowles
  • NKT-like cell subpopulation and method of using the same in the treatment of tumor
    Patent number: 10149863
    Abstract: The disclosure discloses a kind of new NKT-like cell subpopulation, a therapeutical composition comprising the NKT-like cell subpopulation, and the medical use thereof. The disclosure also provides a preparation method of the NKT-like cell subpopulation. The disclosed NKT-like cell subpopulation has a strong antitumor effect, and can be adoptive transferred into a subject to treat the tumor in the subject after in vitro cultured and amplified.
    Type: Grant
    Filed: August 8, 2016
    Date of Patent: December 11, 2018
    Assignee: TSINGHUA UNIVERSITY
    Inventor: Minghui Zhang
  • Method for measuring indoxyl sulfuric acid
    Patent number: 10150983
    Abstract: It is an object of this invention to provide a simple measurement method capable of detecting indoxyl sulfuric acid in a sample rapidly and at high sensitivity. By causing sulfatase and tetrazolium salt to act on indoxyl sulfuric acid in a sample to generate a formazan dye, and then calculating the generation amount of the formazan dye, indoxyl sulfuric acid in the sample can be measured more simply, more rapidly, and at higher sensitivity as compared with former methods.
    Type: Grant
    Filed: February 18, 2014
    Date of Patent: December 11, 2018
    Assignee: Nipro Corporation
    Inventors: Kenta Tatsumi, Daisuke Nakamura
  • Systems and methods for electrochemical aspartate transaminase (AST) and alanine transaminase (ALT) detection and quantification
    Patent number: 10144949
    Abstract: A system for electrochemically detecting AST and ALT includes a first sampler for producing pyruvate from ALT and a second sampler for producing pyruvate from AST. The system further includes an electrochemical test strip for receiving processed samples from the first and second samplers, the processed samples containing pyruvate. The system further includes a meter for reading the electrochemical test strip and indicating an amount of AST and ALT in the sample.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: December 4, 2018
    Assignee: Polymer Technology Systems, Inc.
    Inventors: Gary L. Hughes, Aniruddha Patwardhan
  • Process for the production of organic compounds from plant species
    Patent number: 10138505
    Abstract: This invention relates to a process for the production of fermentable C5-C6 sugars from oleaginous herbaceous plants comprising the steps of: a) mechanically separating the seeds from the above-ground lignocellulose biomass and breaking-up said lignocellulose biomass; b) placing the above-ground lignocellulose biomass in contact with a basic aqueous solution so as to obtain a paste containing from 10 to 50% by weight of the said lignocellulose biomass at temperature of between 10 and 95° C. for a time of between 1 minute and 24 hours; c) separating out the paste obtained in step b) in a solid fraction containing essentially hemicellulose and cellulose; in a liquid fraction containing lignin and extractables; d) subjecting the said solid fraction containing essentially hemicellulose and cellulose to enzyme hydrolysis.
    Type: Grant
    Filed: March 20, 2013
    Date of Patent: November 27, 2018
    Assignee: Novamont S.p.A.
    Inventors: Catia Bastioli, Giampietro Borsotti, Luigi Capuzzi
  • Simple production method for graphene by microorganisms
    Patent number: 10138126
    Abstract: Systems and methods are provided for producing graphene from graphene oxide in an environmentally friendly, cost effective and simple process, which uses microorganisms as a reducing agent to achieve the desired result. The systems and methods avoid the use of toxic or environmentally harmful reducing agents commonly used as reducing agents in the chemical reduction of GO to obtain graphene.
    Type: Grant
    Filed: May 26, 2015
    Date of Patent: November 27, 2018
    Inventors: Mariam Al Ali Alamaadeed, Noorunnisa Khanam Patan
  • Method for preparing diglyceride using bubble column reactor
    Patent number: 10138501
    Abstract: Disclosed is a method for synthesizing diglyceride using a bubble column reactor. The method comprises the steps of: an immobilized enzyme is placed on the bearing mechanism of the bubble column reactor; a hot bath mechanism is actuated to heat the reactor body to 55-75° C.; glycerol, fatty acid and water are added into a feed chute, preheated to 55-75° C.
    Type: Grant
    Filed: July 24, 2015
    Date of Patent: November 27, 2018
    Assignee: Jinan University
    Inventors: Yong Wang, Yinglai Teng, Manman Liu, Ning Zhang
  • Protective agent for electron microscopic observation of biological sample in water-containing state, kit for electron microscopic observation, methods for observation, diagnosis, evaluation, and quantification by electron microscope, and sample stage
    Patent number: 10128078
    Abstract: Provided are a protective agent for electron microscopic observation in a vacuum which can protect a biological sample in a water-containing state, such as a mammal, a plant tissue or a cultured cell, and a single cell in the living state without deforming it even in a vacuum, a kit using the same, methods for observation, diagnosis, evaluation, and quantification of a sample by an electron microscope, and a sample stage to be used for the observation. The protective agent for electron microscopic observation of the present invention contains a a component to impart the survival environment, a saccharide, and an electrolyte.
    Type: Grant
    Filed: January 28, 2015
    Date of Patent: November 13, 2018
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Takahiko Hariyama, Yasuharu Takaku, Hiroshi Suzuki, Satoshi Hirakawa, Hideaki Kawasaki, Masatsugu Shimomura, Daisuke Ishii, Isao Ohta, Yoshinori Muranaka
  • Methods and systems for reducing one or more impurities and/or moisture from grain oil, and related compositions
    Patent number: 10113187
    Abstract: The present disclosure relates to methods and systems for injecting a gas (e.g., air) into a grain oil recovered from a grain process so as to remove an amount of one or more materials (e.g., moisture, unsaponifiables, insolubles, free fatty acids, and the like) from the grain oil.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: October 30, 2018
    Assignee: Poet Research, Inc.
    Inventors: David D. Bushong, Casey C. Jenks
  • Diagnostic methods for neural disorders
    Patent number: 10107796
    Abstract: The invention generally relates to optical methods for the diagnosis of neuronal condition by converting a cell from a patient into a neuron and optically evaluating action potentials of that cell in vitro. The cell is transformed with an optical reporter and exhibits an optical signature in response to neural stimulation. Using genome-editing, a control cell can be made that is isogenic but-for a known mutation and a control signature obtained from the control cell. Thus, methods of the invention reveal potential neurodegenerative effects of a mutation as manifested in a patient's genetic context. The optical signature of the cell, or the difference between the signature and the control signature, is correlated to a diagnosis of the neurodegenerative disease.
    Type: Grant
    Filed: January 4, 2017
    Date of Patent: October 23, 2018
    Assignee: Q-STATE BIOSCIENCES, INC.
    Inventors: Kevin C. Eggan, Adam Cohen, Joel Kralj, Evangelos Kiskinis
  • System and method for detecting lysyl oxidase-like 2 protein (LOXL2) and breast cancer
    Patent number: 10107778
    Abstract: A detection system for determining lysyl oxidase-like 2 protein (LOXL2) levels in a bodily sample, includes at least one reaction solution for generating H2O2 upon combination with LOXL2 in the bodily sample, the reaction solution including lysine that can be oxidatively deaminated by LOXL2 to generate ?-aminoadipic-?-semialdehyde (allysine) and hydrogen peroxide (H2O2).
    Type: Grant
    Filed: September 30, 2013
    Date of Patent: October 23, 2018
    Assignee: CASE WESTERN RESERVE UNIVERSITY
    Inventors: Chung-Chiun Liu, Yuan Zhang, Metini Janyasupab, Cheng-Wei Liu, Laurie Dudik
  • Tissue processing reagent
    Patent number: 10107727
    Abstract: A method including placing a biological sample taken from a body into a chamber; adding a composition including an acetal solvent to the chamber; and fixating the biological sample. A method including placing a tissue from a body into a chamber; and contacting the tissue with a composition including an acetal solvent as a fixating process.
    Type: Grant
    Filed: December 7, 2016
    Date of Patent: October 23, 2018
    Assignee: Sakura Finetek U.S.A., Inc.
    Inventor: Amit D. Shah
  • Method for the differential enumeration of lactic acid bacteria in a mixture in a food product
    Patent number: 10093958
    Abstract: The invention relates to a method for distinguishing between and enumerating strains of lactic acid bacteria or Bifidobacteria present in a food product. This method implements various agar culture media and/or selective culture conditions, combined with various chromogenic substrates.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: October 9, 2018
    Assignee: Compagnie Gervais Danone
    Inventors: Candice Fourmestraux, Jerôme Combrisson, Mickaël Boyer, Anna Galat, Leyla Boumghar-Bourtchai
  • Method for removal of triglycerides in lipoproteins other than low-density lipoproteins
    Patent number: 10077286
    Abstract: Disclosed is a method for selectively eliminating triglycerides in lipoproteins other than low density lipoprotein, which method allows one to provide a method for directly and differentially quantifying LDL-TG in a sample with excellent simplicity, specificity and accuracy using an automated analyzer or the like without performing a laborious operation of pretreatment such as centrifugation or electrophoresis. The method for eliminating triglycerides in lipoproteins other than low density lipoproteins includes allowing lipoprotein lipase, cholesterol esterase, glycerol kinase and glycerol-3-phosphate oxidase to act on a sample in the presence of a surfactant that acts on lipoproteins other than low density lipoprotein and/or a surfactant having LDL-protecting action, and eliminating hydrogen peroxide produced thereby.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: September 18, 2018
    Assignee: DENKA SEIKEN CO., LTD.
    Inventor: Motoko Ohta
  • Composition and method for delivery of living cells in a dry mode having a surface layer
    Patent number: 10047339
    Abstract: The present invention generally relates to compositions and methods of delivering living cells in a dry mode, wherein the compositions include a surface layer disposed on the outer surface of the composition that is permeable to carbon dioxide and oxygen. The compositions may be used to deliver living cells to a delivery point without the use of expensive refrigerants such as dry ice or liquid nitrogen.
    Type: Grant
    Filed: March 10, 2016
    Date of Patent: August 14, 2018
    Assignee: DRYLET, LLC
    Inventors: Ramiro Trevino, Steven R. Ellis
  • Method of mixotrophic culturing of microalgae in a flexible bioreactor
    Patent number: 10047337
    Abstract: Some embodiments include a method of culturing one or more microalgae. The method can include: inoculating a bioreactor with the one or more microalgae and a fluidic support medium, the bioreactor having one or more bioreactor walls at least partially enclosing a bioreactor cavity, being configured to be at least one of folded up or rolled up, and being sterile when the bioreactor is inoculated with the one or more microalgae, the one or more bioreactor walls comprising at least one bioreactor wall material, and the at least one bioreactor wall material being flexible and at least partially transparent; and vitally supporting the one or more microalgae. Other embodiments of related systems and methods are also disclosed.
    Type: Grant
    Filed: April 29, 2016
    Date of Patent: August 14, 2018
    Assignee: Heliae Development LLC
    Inventors: Luke Cizek, Miquel Olaizola, Mason McCarty, Mason Oelschlager