Abstract: Asporous mutants of Bacillus thuringiensis serovar. israelensis, a process for their preparation and their use for the isolation of bacterial toxins for controlling diptera.

Abstract: A method of multiplying bovine embryos by transfer of nuclei is described. The method includes enucleating a recipient oocyte, transferring the donor embryo nucleus to the enucleated recipient oocyte, and fusing the donor nucleus to the recipient oocyte by dielectrophoresis. The method is intended to allow the generation of multiplied genetically identical cattle.

Abstract: Vigorous growth of endothelial cells from human umbilical vein endothelial cells in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.

Abstract: A method for regenerating soybean plants from cotyledonary nodes is disclosed. Soybean seeds are germinated on nutrient medium containing a cytokinin to produce a donor plant. The cotyledonary nodes of the donor plant are excised and divided into pieces. The cotyledonary node pieces are cultured on nutrient medium containing a cytokinin until callus tissue develops which contains shoots. The shoots are removed and rooted on nutrient medium free of exogenous hormone to form a plantlet.

Abstract: A transacting DNA binding factor is disclosed. The ASF-1 protein factor specifically binds to the sequence motif TGACG found upstream of the promoter in many plant genes. Co-expression of this protein factor augments the level of expression of the up-regulated promoter containing the TGACG motif.

Abstract: A method is disclosed for the in vitro growth and development of cells obtained from the follicles of a mammal to reproductively competent oocytes. By incubating these cells, which comprise small and medium oocytes, in a series of specific hormone-supplemented tissue culture media as described herein, they can be induced to grow and develop into reproductively competent oocytes. After a further maturation step in which chromosome number reduction occurs, the oocytes are fertilizable.

Abstract: Phosphinamides are prepared by the action of an organic halide on an oxazaphospholidine. Depending upon the configuration desired for the stereoisomer to be obtained, an oxazaphospholidine is utilized which has been derived from a (+) or (-) optically-active amino-alcohol. These phosphinamides are useful for the preparation of phosphinates, in particular optically-active phosphinates of known absolute configuration; for this, the phosphinamide is subjected to alcoholysis.

Abstract: Recombinant DNA clones according to the invention contain nodABCDIJL genes of B. japonicum. These clones can be used to practice a method wherein a nod- strain of Rhizobium or Bradyrhizobium bacteria lacking one or more of these genes is converted to a nod+ strain by transfering one or more of the missing genes to the strain. According to a further aspect of the invention, nucleotide sequences have been identified which control the expression of nod LABC genes of Bradyrhizobium japonicum in the presence of flavones produced by the plant roots. By linking such sequences with a gene having a desired characteristic, such as a gene encoding for production of a plant parasite toxin, bacterial can be created which selectively express the characteristic only in the presence of the flavone which triggers expression of the gene.

Abstract: A method for the combined in-line bleaching and dewaxing of vegetable oils which includes the steps of bleaching the vegetable oil with a sufficient amount of bleaching clay and filter aid at a temperature of about 80.degree.-130.degree. C. for about 15-60 minutes, followed by rapid cooling of the bleached vegetable oil containing the bleaching clay, to a temperature of about 0.degree.-15.degree. C. for about 15 minutes-4 hours to thereby dewax the vegetable oil. The spent bleaching clay, waxy material and other impurities in the vegetable oil are then seaparted at low temperatures of about 0.degree.-20.degree. C., by such means as filtration, to thereby recover the bleached and dewaxed vegetable oil.

Abstract: A process for the one-step conversion of cephalosporin C and derivatives thereof to the corresponding 7-aminocephalosporanic acid and derivatives comprising treating said cephalosporin C and derivatives with a cephalosporin C amidase derived from Arthrobacter viscosus ATCC 53594, or from any cephalosporin C amidase producing, or potentially producing descendants thereof, or from any expression of the genetic material of said Arthrobacter viscosus ATCC 53594, or any cephalosporin C amidase producing, or potentially producing descendants thereof.

Abstract: Immortalized human uroepithelial cells are disclosed. One cell line is not spontaneously tumorigenic, but can be tumorigenically transformed by certain carcinogens. Another is a tumorigenic variant that provides a source of epithelial keratins.

Abstract: There is disclosed a method of treating or preventing viral infections in plants wherein an animal interferon is applied to the plants.

Abstract: Immunoreactive heterochain antibodies are described. The heterochain antibodies are made up of light chain and heavy chain variable regions derived from different antibodies. The heterochain antibodies can exhibit antigen binding properties which are different from the parent antibodies from which they are derived. Methods of producing the heterochain antibodies and methods of their use in diagnostic and therapy are also disclosed.

Abstract: A new process for the production of tocopherols by tissue culture, which comprises (1) preparing a callus of a plant Carthamus tinctorius, (2) inoculating the callus into a synthetic nutrient medium and culturing the callus to produce tocopherols, and (3) recovering the tocopherols. The tocopherols thus produced are minaly .alpha.-tocopherol, which has the strongest vitamin E activity among tocopherol analogs.

Abstract: 2,2'-Dichloro-6,6'-diethylmethylene-bis-aniline or mixtures thereof with compounds of the formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4 are the same or different and are straight-chained or branched alkyl radicals having 2 to 4 C atoms, or R.sub.1 and/or R.sub.3 are chlorine and the remaining R's have the above-mentioned significance, as chain-extension means or cross-linkage means for polyurethanes or epoxide resins. Process for the production of 2,2'-dichloro-6,6'-diethylmethylene-bis-aniline or such mixtures. 2-ethyl-6-chloroaniline is condensed with itself or an aniline having the formula: ##STR2## wherein R.sub.5 and R.sub.6 are the same or different and are straight-chained or branched having 2 to 4 C atoms, with the proviso that R.sub.5 can also be chlorine, in acidic medium with formaldehyde or compounds which form formaldehyde. The compounds in the mixture can be condensation products of two molecules of such aniline derivatives.

Abstract: Disclosed is cultured plant cell derived from tissues or cells of a plant belonging to Hydrocotyle genus (water-pennyworts) and Centella genus, a culture method therefor, and a blood coagulation component and an therapeutic agent for mental disease obtained the cultured plant cells.

Abstract: A DNA sequence on which a chromosomal DNA sequence coding for human interferon-.gamma. (HuIFN-.gamma.) and having a TATA box is ligated to a sequence of a promoter. Cell cultures transformed with said DNA revealed a higher expression of HuIFN- than cell cultures transformed with a DNA which does not have such TATA box. When transformed with said DNA, cell cultures produced HuIFN-.gamma. in a serum free medium. Transformed cell cultures derived from blood cells of HuIFN-.gamma. resistant human cell-line also could produce HuIFN-.gamma..

Abstract: Transgenic plants are disclosed which are resistant to virus infection by Potato Virus X and Potato Virus Y. Plant genes and transformation vectors are also disclosed. Potato plants, for example, Russet Burbank variety, are made resistant to dual infection by Potato Virus X and Potato Virus Y by transforming the plant to express the coat proteins of the two viruses.

Abstract: Microorganisms have been developed which may be characterized as possessing substantially all of the following qualities or capabilities:(a) capable of having foreign genetic information introduced thereinto and recovered therefrom along with its expression with production of useful gene products;(b) the microorganism being dependent for growth and survival upon defined conditions;(c) the microorganism being incapable of establishment or growth or colonization and/or survival under conditions or in ecological niches that are considered to be natural and/or undesirable for said microorganism;(d) the microorganism being capable of causing genetic information incorporated therein to undergo degradation under conditions or ecological niches that are considered to be natural and/or undesirable for said microorganism;(e) the microorganism being capable of permitting cloning vectors incorporated therein to be dependent for their replication, maintenance and/or function on said microorganism;(f) the microorganism bei

Abstract: This invention provides a continuous bioconversion process in which a non-growth toluene substrate is bio-oxidized by a specific microbe mutant strain to accumulated extracellular muconic acid at a bioreactor production rate of at least about 5 grams of muconic acid per liter of fermentation medium per hour.Essential features of the invention process include a continuous feed of whole cell-containing fermentation broth from an auxiliary cell growth and enzyme induction fermentation zone into the main fermentation zone, and a purge stream of whole cell-containing fermentation broth from the main fermentation zone.