Abstract: A rapid immunoassay method and apparatus for detecting foot and mouth disease virus are disclosed. The method and test device permit pen-side testing of animals and provide test results within a relatively short time period. In a preferred embodiment, the method and apparatus provide a means for differentiating between FMDV-infected and FMDV-vaccinated animals.
Type:
Grant
Filed:
April 1, 2010
Date of Patent:
October 4, 2016
Inventors:
Je-Mo Kang, Nam-Kyu Shin, In-Soo Cho, Bang-Hun Hyun, Kwang-Nyeong Lee, Jae-Ku Oem, Soo-Jeong Kye, Young-Joon Ko, Bok-Kyung Ku, Soo-Hwan An, In-Joong Kim, Ok-Kyung Kim, Hee-Jong Kim, Ki-Yong Jang, Suh-Ha Hwang, Chang-Ho Kim, Song-Woo Ko, Yi-Seok Joo
Abstract: A method and device for the rapid detection of biomolecules (320) diffusing in a nanometer-confined slit (204) is claimed. In particular, the present invention relates to a novel concept of fluidic side apertures (205) that facilitates the filling of the device, the surface coating with biomolecules and that measures the affinity between fluorescently labeled biomolecules in aqueous solution with other biomolecules immobilized on surfaces.
Type:
Grant
Filed:
November 19, 2010
Date of Patent:
September 27, 2016
Assignee:
ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)
Inventors:
Nicolas Durand, Yannick Fournier, Theo Lasser, Iwan Märki
Abstract: Provided is a method of analyzing binding efficiency of adhesive nanoparticles. The method includes (a) injecting a solution containing nanoparticles into a first chamber slide, (b) evaporating only the solution from the first chamber slide into which the solution containing the nanoparticles is injected, and measuring a saturation temperature using a thermal imager while radiating light from a light source, (c) injecting cells into a second chamber slide, (d) injecting a solution containing nanoparticles into the second chamber slide in which the cells are cultured, (e) removing nanoparticles which are not bound to the cells from the second chamber slide into which the cells and the nanoparticles are injected, and (f) evaporating only the solution from the second chamber slide from which the nanoparticles are removed, and measuring a saturation temperature using a thermal image while radiating light from the light source.
Type:
Grant
Filed:
May 16, 2013
Date of Patent:
September 13, 2016
Assignee:
KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
Inventors:
Young Min Jhon, Chi Woong Jang, Myung Suk Chun, Seok Lee, Jae Hun Kim, Chul Ki Kim, Deok Ha Woo, Taik Jin Lee, Young Tae Byun, Sun Ho Kim
Abstract: The invention relates to a sensing device for sensing a fluid. The sensing device comprises an inlet port (20) for receiving the fluid (100) and a measurement chamber (25) for sensing the fluid (100). The sensing device further comprises a fluid channel (30) coupling the inlet port (20) and the measurement chamber (25) for transporting the fluid (100) from the inlet port (20) to the measurement chamber (25) and a fluid stop unit (50) for stopping and controllably releasing the flow of fluid between the inlet port (20) and the measurement chamber (25). This allows stopping the fluid before reaching the measurement chamber (25) and controllably releasing the fluid for allowing the fluid to flow into the measurement chamber (25), if the sensing device is ready for analyzing the fluid.
Type:
Grant
Filed:
August 5, 2014
Date of Patent:
August 30, 2016
Assignee:
KONINKLIJKE PHILIPS N.V.
Inventors:
Albert Hendrik Jan Immink, Jozef Franciscus Elisabeth Gootzen, Dirk Jan Nelly Vananderoye, Petrus Johannes Wilhelmus Van Lankvelt
Abstract: The present invention relates to a diagnostic method for the detection of small quantities of amniotic fluid in the vagina. More specifically, the invention relates to the detection of PAMG-1 in the vagina using anti-PAMG-1 antibodies.
Abstract: Methods of determining whether an analyte is present in a sample using a competitive assay are provided. Aspects of the methods include employing a competitor that provides for the obtainment of signal which is directly proportional to the amount of analyte in the sample. Also provided are devices and kits that find use in practicing the methods described herein.
Abstract: A device for determining the presence and/or quantity of one or more analytes in a sample of human body fluid has a container for receiving a sample of body fluid, with an interior that is delimited by a base and by a circumferential surface. It further comprises at least one test strip and a holding element for receiving and holding the one or more test strips. The holding element is designed such that it has a shape corresponding and adapted to the peripheral circumferential surface of the container. The device further comprises an elongate sampling element having an absorbent sampler that takes up the sample of body fluid and by means of which the sample of body fluid is transferred into the container. The sampling element can include an indicator strip for determining whether the amount of liquid sample sufficient for carrying out an assay.
Type:
Grant
Filed:
September 29, 2014
Date of Patent:
August 16, 2016
Assignee:
EXPRESS DIAGNOSTICS INT'L, INC.
Inventors:
Matthias W. Engel, Leslie Wilson, Paul Johnson
Abstract: One or more aqueous, near infrared emitting, high yield, highly photoluminescent, stable quantum dots conjugated to one or more biomarkers specific moieties. The conjugated quantum dots have an enhanced detection sensitivity and selectivity and may be formed using a novel and efficient method for conjugating one or more biomarker specific moieties to the quantum dots. The invention is further directed to a method for using the conjugated quantum dots for cancer detection in the margin of excised tissue.
Type:
Grant
Filed:
May 25, 2012
Date of Patent:
August 16, 2016
Assignee:
Drexel University
Inventors:
Wei-Heng Shih, Wan Y. Shih, Giang Au, Ari D. Brooks, Vanlila K. Swami
Abstract: Methods for forming cell arrays of multiple cell samples arranged substantially in a monolayer on a single substrate particularly suited for diagnostic analysis are disclosed. The cell arrays are formed with a high-speed dispensing apparatus capable of dispensing small volumes in precise, complex patterns. Also disclosed are substrates upon which cell arrays may be formed, and methods for conducting diagnostic analyses on the formed cell arrays.
Type:
Grant
Filed:
December 23, 2014
Date of Patent:
August 16, 2016
Assignees:
Accupath Diagnostic Laboratories, Inc., BioDot, Inc.
Inventors:
Mathew Moore, Miriam Reyes, David Baunoch, Thomas C. Tisone, Brendan O'Farrell
Abstract: A laminated, integrated, analyte assay device and methods of using the device are described. The assay device is useful as an inexpensive, disposable assay device for detecting the presence and/or amount of a particular analyte in a body fluid or other sample.
Abstract: Methods for detecting one or more analytes, such as a protein, in a fluid path are provided. The methods include resolving, immobilizing and detecting one or more analytes in a fluid path, such as a capillary. Also included are devices and kits for performing such assays.
Type:
Grant
Filed:
November 19, 2010
Date of Patent:
July 26, 2016
Assignee:
ProteinSimple
Inventors:
Tom Weisan Yang, Arunashree Bhamidipati, Andrei V. Bordunov, James Eugene Knittle, Roger A. O'Neill, Karl O. Voss
Abstract: The present invention provides a single self-contained device for collecting, extracting, on-site testing, and transferring for forensic confirmatory analysis, a wide variety of substances including, but not limited to, drugs of abuse, explosives, weapons of mass destruction, food toxins and industrial wastes. Samples can be obtained from a surface by swabbing a suspect area or the testing of solid materials (pills, capsules, powders), air samples and biological and non-biological fluids by placing the substance in the device. The device includes a swab, a retention well including a wash, and analysis technologies that can be, for example, a lateral flow testing system. The swab is rinsed with a wash prior to testing thereby not compromising the chemistry of the detection technologies and allowing for a wide variety of applications under a number of field conditions. Also, the device is a single self-contained unit instead of having a separate reagent droppers or sprays, making it compact and easy to use.
Type:
Grant
Filed:
February 11, 2013
Date of Patent:
June 7, 2016
Assignee:
University of Florida Research Foundation, Inc.
Inventors:
Mark S. Gold, David M. Martin, Steven T. Gold, Bruce A. Goldberger
Abstract: A magnetic particle based separation and assaying method uses at least two sets of magnetic particles placed in solution within a container and characterized respectively by a coercive field e1 and e2, wherein e1 is greater than e2. The first magnetic particles with a larger coercive field e1 will be used as carrier to handle the second affinity magnetic particles having a lower coercive field e2. A magnetic particles handling method includes the step of applying an external magnetic field having a polarity and amplitude that varies with time to cause the said carrier magnetic particles to be in relative motion within the container driving thereby the affinity particles to form an homogenous suspension of particles in perpetual relative movement with the respect to the liquid.
Abstract: The invention relates to a laboratory apparatus for handling at least one laboratory sample, in particular for mixing and/or adjusting the temperature of a biochemical laboratory sample, which is arranged in at least one sample vessel element, having a carrier device for carrying the at least one sample vessel element, an electrical control device, which is set up for controlling or setting at least one operating parameter of the laboratory apparatus that controls this handling, and at least one sensor device for recording at least one measured value, by which at least one geometrical property of the at least one sample vessel element can be determined, the at least one sensor device being signal-connected to the electrical control device, the electrical control device being set up for controlling the handling of the at least one laboratory sample in dependence on the at least one measured value and the at least one set operating parameter by at least one control step.
Type:
Grant
Filed:
August 2, 2012
Date of Patent:
May 31, 2016
Assignee:
EPPENDORF AF
Inventors:
Florian Duerr, Ruediger Huhn, Manuel Mayer, Janine Roehrs, Gerrit Walter, Wolf Wente
Abstract: Devices and methods for the detection of target molecules are disclosed. Devices and methods for detecting food-borne target molecules are also disclosed.
Type:
Grant
Filed:
June 28, 2013
Date of Patent:
May 17, 2016
Assignee:
Invisible Sentinel, Inc.
Inventors:
Nicholas A. Siciliano, Martin Joseph Bouliane
Abstract: An assay device for determining the presence of analytes in a cell lysate comprises a porous support member and a plurality of binding reagents arranged and immobilized at multiple reaction sites on the support member. The binding reagents are selected and arranged to assess the status of a selected cellular signal transduction pathway/protein-protein interactive network. In a further aspect, a method for assessing the status of a signal transduction pathway comprises generating a lysate of cells, the lysate retaining one or more pathway molecules present in one or more states and the pathway molecules reflecting signal transduction events taking place in the cells. The method further includes applying the lysate to an immobilized series of binding reagents which can discriminate the pathway molecules and their states. Binding events between the pathway molecules and the binding reagents are identified and the state of the selected signal pathway is determined.
Type:
Grant
Filed:
February 2, 2001
Date of Patent:
May 10, 2016
Assignee:
Instant Medical Diagnostics, LLC
Inventors:
Lance A. Liotta, Emanuel F. Petricoin, III, Katherine L. Paweletz, Alan R. Day
Abstract: An assay method with use of a sensor chip which includes a metal member, a self-assembled monolayer (SAM), and ligands on a support, and is configured to be used for a fluorescence measuring apparatus with utilization of a surface plasmon-field enhanced Fluorescence Spectrometry, including the steps of: forming a hydrophilic high molecule layer on the self-assembled monolayer in the sensor chip; immobilizing the ligands at least one of in the hydrophilic high molecule layer and on the surface of the hydrophilic high molecule layer; and bringing a moisturizer in contact with the hydrophilic high molecule layer.
Abstract: Light-emitting intra-cavity interferometric (ICI) optical sensors based on channel waveguide structures which include an internal light emitting material and a functionalized region. In some embodiments, the waveguides are made of a sol-gel which incorporates the light emitting material. In some embodiments, the waveguide structure includes an ICI resonator backbone and the ICI sensor is a laser sensor. In some embodiments, the resonator backbone has an interferometric Y-branch shape. In some embodiments, the resonator backbone has a Mach Zehnder interferometer shape. In some embodiments, an ICI laser sensor has an interferometric arrayed waveguide grating shape. In some embodiments, an ICI sensor may be remotely optically pumped and remotely read.
Type:
Grant
Filed:
June 3, 2012
Date of Patent:
April 19, 2016
Assignee:
Tel Aviv University Future Technology Development Ltd.