Abstract: The present invention provides a vertical gas distribution device for aerobic compost and a gas distribution method. Current gas distribution pipelines are easily blocked by organic materials, resulting in poor ventilation due to non-uniform local ventilation and cleaning difficulties. The inventive device comprises a compost chamber, a compost bin, a compost heap, a percolate tank, and a plurality of gas distribution branch pipes at the bottom of the compost chamber, the pipes being vertically movable in the chamber; the percolate tank is positioned at the bottom of the chamber and under the pipes; an air inlet is provided on the side wall of the tank, and an outlet is provided on the side wall of the chamber; the compost bin is inside the compost chamber, and the compost heap is in the compost bin; each pipe is top sealed, with a plurality of vent holes on the side wall, and its bottom connected to the tank. The present invention is usable for compost ventilation.

Abstract: A cell culture device includes a main body and a plug element. The main body includes a slot, an open groove and a fluid chamber. The open groove is connected with one side of the slot. The fluid chamber is disposed inside the main body and connected with another side of the slot. The plug element includes a first cell culture chamber and a first porous membrane. The first porous membrane is disposed at one side of the first cell culture chamber. The plug element is detachably plugged into the slot. When the plug element is plugged into the slot, the first cell culture chamber is communicated with the open groove to form an open space, and the open space and the fluid chamber are separated by the first porous membrane.

Abstract: A collection pipette that collects a microscopic object includes a first tube part, a second tube part connected to an end of the first tube part, and a third tube part connected to the other end of the first tube part. The longitudinal direction of the third tube part intersects with the longitudinal direction of the first tube part, and is parallel to the longitudinal direction of the second tube part. For example, the length in the longitudinal direction of the third tube part is shorter than the length in the longitudinal direction of the first tube part.

Abstract: Described herein are microfluidic devices and systems for high density cell culture and/or high throughput cell assays. Methods of using the same are also provided herein. In some embodiments, the microfluidic devices and systems described herein provide rapid and automated trapping of single embryos in ordered arrays.

Abstract: An incubator (110, 140) for the reception of an individual sample carrier, a shelving system (100) for such incubators, a transport container for such incubators, as well as a modular system are suggested, wherein the incubators can be inserted and withdrawn (130) independently of one another, can be supplied with electricity, water and a gas via corresponding connections (150), and can communicate with a computer network via a wireless connection. Such a system reduces the risk of contamination or confusion in the lab and avoids interruptions in the monitoring and controlling of the environmental conditions in the respective incubators. These can rather independently maintain the most favourable environmental conditions for the respective samples, both during storage and also during a transport operation. The insertion and withdrawal of individual incubators from the shelving system does not, or only very briefly, interrupt the monitoring and control of the conditions in the interior of the incubators.

Abstract: The present disclosure relates to a device and method for remedying a heavy-metal-polluted soil with a microorganism, and relates to a device for remedying a metal-polluted soil and a remediation method thereof. The present disclosure solves the problem that a remediation microorganism cannot be extracted from the soil. The device comprises a main plate and a plurality of microorganism-extracting chambers, wherein the main plate is connected to the microorganism-extracting chambers; the microorganism-extracting chamber are filled with a filler; a tail end of the microorganism-extracting chambers is spiky, and openings are separately provided on a side wall of the microorganism-extracting chambers. The method comprises: I, adding a nutrient medium to the soil, and then inoculating a remediation microorganism; and II, inserting the above device into the soil for 6˜8 days, and then taking it out. The method can remedy heavy-metal-polluted soil in a short time with a high removal rate of the heavy metal.

Abstract: The present invention provides a method for culturing cells in a cell culture container having a base section, a top section arranged in parallel with the top section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, and in which the top section of the container has an optionally sealable inlet, in which the container is composed of a flexible material, comprising culturing cells in a culture medium in the cell culture container.

Abstract: A thermal block assembly is provided. The assembly can comprise a substrate comprising a first surface configured with a plurality of reaction sites each reaction site configured to contain a biological sample and a sample block comprising a plurality of pedestals configured to thermally modulate the plurality of biological samples wherein each pedestal is thermally coupled to one of the reaction sites. The assembly can further comprise cooling blocks, slots and insulating rings associated with reaction sites each capable of minimizing heat flow between reaction sites. A method for thermally isolating reaction sites is also provided.

Abstract: The present invention provides a cell culture vessel or a sample cell for observation use that makes it possible to observe three-dimensionally-cultured cells from various angles. The cell culture vessel or the sample cell for observation use according to the present invention is characterized by being equipped with a culture gel in which a cell or cell tissue is embedded and a first vessel which encloses the culture gel, wherein a space in the first vessel is filled with the culture gel; and the first vessel has a light-permeable window made from a hydrogel.

Abstract: Disclosed are an apparatus and methods for rapid amplification of nucleic acids. More particularly, the present disclosure relates to an apparatus for mixing a reaction solution during amplification of nucleic acids and to methods for amplifying nucleic acids. Also disclosed are methods for lysing cells in a sample and amplifying nucleic acids.

Abstract: The present invention provides various improved systems and methods for obtaining, generating, culturing, and handling cells, such as stem cells (including induced pluripotent stem cells or iPSCs) and differentiated cells, as well as cells and cell panels produced using such systems and methods, and uses of such cells and cell panels.

Abstract: The present invention provides for methods and at least partially automated devices suitable for producing a transplantable cellular suspension of living tissue suitable for promoting tissue regeneration in an epithelium-related procedure, as well as compositions produced therefrom. Tissue regeneration in humans is extremely limited and constitutes a major challenge to the repair of damaged organ function. Wound treatment is a typical area where tissue regeneration is required. Wounds (lacerations or openings) in mammalian tissue can result in tissue disruption and coagulation of the microvasculature at the wound face.

Abstract: Generation of energy and storage of energy for subsequent use is provided by electromethanogenesis of carbon dioxide into a fuel gas and the storage of the fuel gas for subsequent use. An electromethanogenic reactor includes an anode conductor and a cathode conductor wherein the cathode conductor includes submicron to micron scale pores. Electromethanogenesis microbes and/or enzymes are located in the micron scale pores of the cathode electrode conductor. Carbon dioxide is introduced into the electromethanogenic reactor and the electromethanogenesis microbes/enzymes and the carbon dioxide interact and produce a fuel gas. The fuel gas is stored for subsequent use, for example use in power generation.

Abstract: The present invention provides a culture vessel with which cells can be aseptically cultured and aseptically transferred to a place where the cells are to be used (such as an operating room) and from which the cells can be simply taken out. The present invention provides a closed culture vessel including an openable airtight vessel, and a cell culture member disposed in the airtight vessel and including a cell culture surface, in which the cell culture member is provided detachably from the airtight vessel.

Abstract: Embodiments described herein relate to apparatus for temperature modulation of samples. Generally, aspects of the disclosure provide for a portable cooling and heating apparatus which enables a user to visualize a sample during cooling or heating. In one embodiment, the apparatus include a sample block, a plurality of thermoelectric modules coupled to the sample block, and a cooling block coupled to the plurality of thermoelectric modules. In another embodiment, the cooling block is in fluid communication with a fluid reservoir. Other embodiments utilize various insulating materials to influence thermal conductivity between the sample block and the cooling block to provide for enhanced temperature control and modulation.

Abstract: Reactor for enzymatic hydrolysis of a raw material comprising in sequence: i)—a first heat exchanger adapted to heat the raw material supplied to the reactor to a temperature within a range that favours enzymatic hydrolysis, ii)—a reactor comprising plural in reactor chambers connected in series, separated by closable valves, iii)—a second heat exchanger adapted to heat the reaction mixture to a temperature higher than the temperature range favouring enzymatic hydrolysis, the reactor being formed with inclined tubular reactor chambers assembled to form a reactor with vertical axis, the first reactor chamber being the vertically uppermost chamber of the reactor, while at least one reactor chamber is adapted to be stirred with a through-flowing inert gas.

Abstract: Digester (1) having an agitating device (10) and an agitating device (10) and a method for manufacturing an agitating device (10), the agitating device (10) comprising multiple agitator blades (21-29) which agitator blades (11-13) comprise a plurality of blade sections (21-29) angled relative to one another. The blade sections (21-29) of the agitator blade (11-13) are angled relative to one another such that the agitator blade gradient (11-13) decreases with the distance (30) from a central rotational axis (19) increasing to configure the agitator blade in a flow-optimized three-dimensional shape.

Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.

Abstract: A device for treating organic materials having a nozzle base, a roof, a rear wall, and two side walls, delimiting the interior of the device. Extending in the nozzle base are tubes connected to nozzles in the nozzle base, directed toward the interior of the device. The tubes are connected to a process gas apparatus for supplying the tubes with process gas. The process gas apparatus includes a ventilator connected to a pressure chamber for feeding the pressure chamber with process gas. The pressure chamber has at least one outlet connected to at least one of the tubes in the nozzle base to conduct process gas from the pressure chamber into the tubes. The ventilator and the pressure chamber are situated on the roof of the device, in particular in an enclosure. The pressure chamber is connected to the tubes via at least one connecting line in the rear wall.

Abstract: Provided is a culture system comprising a base comprising a multiwell plate; a transwell-like type insert plate comprising a plurality of culture insert assemblies, wherein each culture insert assembly comprises a culture surface element that is removably mounted to a culture insert body; and a cover designed to fit over the transwell-like type insert plate.