Patents Examined by Delia M. Ramirez
  • Microorganism for methionine production with improved methionine synthase activity and methionine efflux
    Patent number: 10196658
    Abstract: The present application is related to a recombinant microorganism optimized for the fermentative production of methionine and/or its derivatives, wherein in said recombinant microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced. The application is also related to a method for optimizing the fermentative production of methionine and/or its derivatives comprising the steps of: c. culturing a recombinant microorganism wherein in said microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and d. recovering methionine and/or its derivatives from the culture medium.
    Type: Grant
    Filed: September 1, 2014
    Date of Patent: February 5, 2019
    Assignee: EVONIK DEGUSSA GMBH
    Inventors: Wanda Dischert, Perrine Vasseur, Rainer Figge
  • Nicotinamide phosphoribosyltransferase (NAMPT) mutant and use thereof
    Patent number: 10174298
    Abstract: The present invention discloses a Nicotinamide phosphoribosyltransferase (nampt) mutant and use thereof. The present invention relates to a nicotinamide phosphoribosyltransferase (Nampt) mutant artificially obtained through genic site-directed mutation. An object of the present invention is to provide a Nampt mutant having a catalytic activity higher than that of a conventional wild type parent, wherein the enzymatic activity of the Nampt mutant provided in the present invention is 1.2-6.9 times of the enzymatic activity of the parent.
    Type: Grant
    Filed: July 30, 2016
    Date of Patent: January 8, 2019
    Assignee: BONTAC BIO-ENGINEERING(SHENZHEN) CO., LTD
    Inventors: Rongzhao Fu, Qi Zhang
  • Heterologous pathway to produce terpenes
    Patent number: 10167488
    Abstract: Cells comprising a heterologous metabolic pathway are configured to produce a terpene product containing non-multiples of five carbon, particularly wherein the pathway comprises heterologous Lepidoptera insect juvenile hormone biosynthetic pathway enzymes of the insect's mevalonate pathway.
    Type: Grant
    Filed: November 3, 2017
    Date of Patent: January 1, 2019
    Assignee: The Regents of the University of California
    Inventors: Jay D. Keasling, Christopher B. Eiben
  • Microorganisms and methods for the production of aniline
    Patent number: 10167477
    Abstract: A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: January 1, 2019
    Assignee: GENOMATICA, INC.
    Inventor: Priti Pharkya
  • Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
    Patent number: 10160983
    Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Grant
    Filed: August 3, 2018
    Date of Patent: December 25, 2018
    Assignee: Codexis, Inc.
    Inventors: Haibin Chen, Steven J. Collier, Jovana Nazor, Joly Sukumaran, Derek Smith, Jeffrey C. Moore, Gregory Hughes, Jacob Janey, Gjalt W. Huisman, Scott J. Novick, Nicholas J. Agard, Oscar Alvizo, Gregory A. Cope, Wan Lin Yeo, Stefanie Ng Minor
  • Modified ornithine decarboxylase protein and a use thereof
    Patent number: 10160981
    Abstract: Provided are a novel modified ornithine decarboxylase protein having improved putrescine productivity and a use thereof.
    Type: Grant
    Filed: July 17, 2014
    Date of Patent: December 25, 2018
    Assignees: CJ Cheiljedang Corporation, Korea Advanced Institute of Science and Technology
    Inventors: Hyang Choi, Hee Kyoung Jung, Hak Sung Kim, Hyun Ho Kyeong, Jung Min Choi, Joong Jae Lee, Hyo Deok Seo
  • Compositions and methods for protein glycosylation
    Patent number: 10150952
    Abstract: Described herein are oligosaccharyl transferases for use in N-glycosylating proteins of interest in vitro and in host cells. Methods for using such oligosaccharyl transferases, nucleic acids encoding such oligosaccharyl transferases, and host cells comprising such oligosaccharyl transferases are also provided herein. Glycoconjugates generated by using such oligosaccharyl transferases are also provided herein.
    Type: Grant
    Filed: December 24, 2015
    Date of Patent: December 11, 2018
    Assignee: GLAXOSMITHKLINE BIOLOGICALS S.A.
    Inventors: Jurgen Haas, Julian Ihssen, Michael Thomas Kowarik, Torsten Franz Schwede, Linda Christiane Thöny-Meyer
  • Mutant organophosphorus acid anhydrolase enzymes with stereospecificity on Sarin enantiomers and uses thereof
    Patent number: 10143874
    Abstract: Disclosed herein are non-wild-type organophosphorus acid anhydrolases having three site mutations, methods of production, and methods of use to effectively degrade toxic chemicals such as((RS)-Propan-2-yl methylphosphonofluoridate)(Sarin) and other organophosphorus compounds.
    Type: Grant
    Filed: September 21, 2017
    Date of Patent: December 4, 2018
    Assignee: The United States of America as Represented by the Secretary of the Army
    Inventors: Sue Y Bae, Mark A. Guelta, Steven P Harvey
  • Engineered nucleic-acid targeting nucleic acids
    Patent number: 10138472
    Abstract: The present disclosure provides engineered cross-type-nucleic-acid targeting nucleic acids and compositions thereof. Nucleic acid sequences encoding the engineered cross-type-nucleic-acid targeting nucleic acids, as well as expression cassettes, vectors and cells comprising such nucleic acid sequences, are described. Also, methods are disclosed for making and using the engineered cross-type-nucleic-acid targeting nucleic acids and compositions thereof.
    Type: Grant
    Filed: July 16, 2018
    Date of Patent: November 27, 2018
    Assignee: Caribou Biosciences, Inc.
    Inventors: Paul Daniel Donohoue, Andrew Paul May
  • Thermostable cellobiohydrolase
    Patent number: 10131892
    Abstract: A thermostable cellobiohydrolase, having a cellobiohydrolase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1 or 2, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 75° C. and pH 5.5, or (C) a polypeptide including an amino acid sequence having 75% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1 or 2, and having hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 75° C. and pH 5.5.
    Type: Grant
    Filed: March 8, 2016
    Date of Patent: November 20, 2018
    Assignee: Honda Motor Co., Ltd.
    Inventors: Jiro Okuma, Migiwa Suda, Asuka Yamaguchi, Yoshitsugu Hirose, Yasuhiro Kondo, Masaru Sato, Daisuke Shibata
  • Engineered nucleic-acid targeting nucleic acids
    Patent number: 10125354
    Abstract: The present disclosure provides engineered cross-type-nucleic-acid targeting nucleic acids and compositions thereof. Nucleic acid sequences encoding the engineered cross-type-nucleic-acid targeting nucleic acids, as well as expression cassettes, vectors and cells comprising such nucleic acid sequences, are described. Also, methods are disclosed for making and using the engineered cross-type-nucleic-acid targeting nucleic acids and compositions thereof.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: November 13, 2018
    Assignee: Caribou Biosciences, Inc.
    Inventors: Paul Daniel Donohoue, Andrew Paul May
  • Ketoreductase polypeptides for the reduction of acetophenones
    Patent number: 10100288
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: March 8, 2018
    Date of Patent: October 16, 2018
    Assignee: Codexis, Inc.
    Inventors: Jack Liang, Stephane J. Jenne, Emily Mundorff, Charlene Ching, John M. Gruber, Anke Krebber, Gjalt W. Hiusman
  • Xylose isomerase genes and their use in fermentation of pentose sugars
    Patent number: 10093914
    Abstract: The present invention relates to eukaryotic cells which have the ability to isomerize xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridium and Fusobacterium or a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.
    Type: Grant
    Filed: April 7, 2016
    Date of Patent: October 9, 2018
    Assignee: DSM IP ASSETS B.V.
    Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
  • Method for manufacturing cis-5-hydroxy-L-pipecolic acid
    Patent number: 10087473
    Abstract: A method for producing cis-5-hydroxy-L-pipecolic acid, the method comprising allowing a 2-oxoglutarate-dependent L-pipecolic acid hydroxylase to act on L-pipecolic acid to generate cis-5-hydroxy-L-pipecolic acid, wherein the 2-oxoglutarate-dependent L-pipecolic acid hydroxylase comprises the polypeptide (A), (B) or (C) below: (A) a polypeptide comprising the amino acid sequence represented by SEQ ID NO: 4 or 11; (B) a polypeptide comprising the amino acid sequence represented by SEQ ID NO: 4 or 11 except that one or several amino acids are deleted, substituted, and/or added, which polypeptide has 2-oxoglutarate-dependent L-pipecolic acid hydroxylase activity; or (C) a polypeptide comprising an amino acid sequence with an identity of not less than 60% to the amino acid sequence represented by SEQ ID NO: 4 or 11, which polypeptide has 2-oxoglutarate-dependent L-pipecolic acid hydroxylase activity.
    Type: Grant
    Filed: November 2, 2015
    Date of Patent: October 2, 2018
    Assignee: API CORPORATION
    Inventors: Ryoma Miyake, Yasumasa Dekishima
  • Polynucleotides encoding cyclohexanone monooxygenases
    Patent number: 10087426
    Abstract: The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess.
    Type: Grant
    Filed: February 23, 2018
    Date of Patent: October 2, 2018
    Assignee: Codexis, Inc.
    Inventors: Ee Lui Ang, Oscar Alvizo, Behnaz Behrouzian, Michael D. Clay, Steven J. Collier, Ellen D. Eberhard, Fu Fan, Shiwei Song, Derek J. Smith, Magnus Widegren, Robert Wilson, Junye Xu, Jun Zhu
  • Flavin-conjugated glucose dehydrogenase
    Patent number: 10077432
    Abstract: A flavin-conjugated glucose dehydrogenase which is composed of proteins having the following amino acid sequence (a), (b) or (c), and having glucose dehydrogenase activity: (a) an amino acid sequence represented by SEQ ID NO: 2, 3, 5, 6, 8 or 9; (b) an amino acid sequence in which one or more amino acids are deleted from, replaced in or added to the amino acid sequence represented by SEQ ID NO: 2, 3, 5, 6, 8 or 9; (c) an amino acid sequence having at least 85% identity with the amino acid sequence represented by SEQ ID NO 2 or 3, at least 95% identity with the amino acid sequence represented by SEQ ID NO 5 or 6, or at least 80% identity with the amino acid sequence represented by SEQ ID NO 8 or 9.
    Type: Grant
    Filed: March 19, 2015
    Date of Patent: September 18, 2018
    Assignee: IKEDA FOOD RESEARCH CO., LTD.
    Inventors: Takafumi Takumi, Mizuki Shigeshiro, Daisuke Sato
  • Compositions and methods for the biosynthesis of vanillin or vanillin beta-D glucoside
    Patent number: 10066252
    Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductase thereby increasing the production of vanillin or vanillin beta-D-glucoside.
    Type: Grant
    Filed: May 15, 2018
    Date of Patent: September 4, 2018
    Assignee: INTERNATIONAL FLAVORS & FRAGRANCES INC.
    Inventors: Esben Halkjaer Hansen, Swee Chuang Lim Hallwyl, Klavs Riishede Hansen
  • Synergistic bacterial consortia for mobilizing soil phosphorus
    Patent number: 10053392
    Abstract: The present disclosure relates to consortia of bacteria strains and composition comprising one or more bacterial strains disclosed herein. These consortia of isolated bacteria cultures and compositions comprising said cultures, having greater activity than would be observed for the individual bacteria cultures and compositions. A composition of the disclosure may advantageously be used for enhancing the availability of soil phosphorus and other macronutrients and/or micronutrients to plants, and thereby enhancing their growth and yield.
    Type: Grant
    Filed: January 12, 2018
    Date of Patent: August 21, 2018
    Assignee: Colorado State University Research Foundation
    Inventors: Matthew D. Wallenstein, Colin W. Bell
  • Functionalized carboxylic acids and alcohols by reverse fatty acid oxidation in engineered microbes
    Patent number: 9994881
    Abstract: Bacteria that run the beta oxidation cycle in reverse anabolic direction are provided, along with many novel primers to start the reverse cycle, pathways to make such primers, and a large variety of products produced thereby. Methods for making desired product by using such primers in the reverse pathway are also disclosed.
    Type: Grant
    Filed: March 6, 2014
    Date of Patent: June 12, 2018
    Assignee: William Marsh Rice University
    Inventors: Ramon Gonzalez, James M. Clomburg
  • Biocatalysts and methods for the synthesis of armodafinil
    Patent number: 9938509
    Abstract: The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess.
    Type: Grant
    Filed: September 7, 2017
    Date of Patent: April 10, 2018
    Assignee: Codexis, Inc.
    Inventors: Ee Lui Ang, Oscar Alvizo, Behnaz Behrouzian, Michael D. Clay, Steven J. Collier, Ellen D. Eberhard, Fu Fan, Shiwei Song, Derek J. Smith, Magnus Widegren, Robert Wilson, Junye Xu, Jun Zhu