Patents Examined by Delia M. Ramirez
  • Microorganisms and methods for the production of aniline
    Patent number: 10612029
    Abstract: A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline.
    Type: Grant
    Filed: November 19, 2018
    Date of Patent: April 7, 2020
    Assignee: GENOMATICA, INC.
    Inventor: Priti Pharkya
  • Use of an esterase to enhance ethyl ester content in fermentation media
    Patent number: 10604776
    Abstract: Methods to prepare vegetable oil compositions having an elevated ethyl ester content are provided.
    Type: Grant
    Filed: May 24, 2018
    Date of Patent: March 31, 2020
    Assignee: Poet Research, Inc.
    Inventors: Alexander T. McCurdy, Steven T. Bly, Stephen M. Lewis, Benjamin P. Gacke, Brandon James Breitling
  • Method of producing proteins in filamentous fungi with decreased CLR1 activity
    Patent number: 10597429
    Abstract: The present invention relates to a method of producing a recombinant polypeptide in a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 1 (CLR1) and to express the recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR1 and to the use of this filamentous fungus in the production of a recombinant polypeptide.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: March 24, 2020
    Assignee: BASF SE
    Inventors: Stefan Haefner, Andreas Thywissen, Holger Hartmann, Nico Boehmer
  • Enzyme scaffolds and methods of use
    Patent number: 10577633
    Abstract: Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: March 3, 2020
    Assignee: Alliance for Sustainable Energy, LLC
    Inventors: Yannick J. Bomble, Michael E. Himmel, Jeffrey Linger, Roman Brunecky, John Aikens
  • Compositions and methods for protein glycosylation
    Patent number: 10577592
    Abstract: Described herein are oligosaccharyl transferases for use in N-glycosylating proteins of interest in vitro and in host cells. Methods for using such oligosaccharyl transferases, nucleic acids encoding such oligosaccharyl transferases, and host cells comprising such oligosaccharyl transferases are also provided herein. Glycoconjugates generated by using such oligosaccharyl transferases are also provided herein.
    Type: Grant
    Filed: November 1, 2018
    Date of Patent: March 3, 2020
    Assignee: GlaxoSmithKline Biologicals SA
    Inventors: Jurgen Haas, Julian Ihssen, Michael Thomas Kowarik, Torsten Franz Schwede, Linda Christiane Thöny-Meyer
  • O-succinylhomoserine producing microorganism and method for producing O-succinylhomoserine using same
    Patent number: 10570429
    Abstract: Provided are a microorganism of the genus Escherichia for producing O-succinylhomoserine and a method for producing O-succinylhomoserine using the same.
    Type: Grant
    Filed: January 22, 2015
    Date of Patent: February 25, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Su Jin Choi, Kuk Ki Hong, Young Lyeol Yang, Min Sun Kang, Hye Min Park, Gyuhyeon Song, Jong Hyun Yoon
  • Metabolically engineered organisms for the production of added value bio-products
    Patent number: 10570430
    Abstract: The present invention relates to genetically engineered organisms, especially microorganisms such as bacteria and yeasts, for the production of added value bio-products such as specialty saccharide, activated saccharide, nucleoside, glycoside, glycolipid or glycoprotein. More specifically, the present invention relates to host cells that are metabolically engineered so that they can produce said valuable specialty products in large quantities and at a high rate by bypassing classical technical problems that occur in biocatalytical or fermentative production processes.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: February 25, 2020
    Assignee: INBIOSE N.V.
    Inventors: Jo Maertens, Joeri Beauprez, Marjan De Mey
  • Recombinant methanotrophic bacterium and a method of production of succinic acid from methane or biogas thereof
    Patent number: 10570424
    Abstract: The present disclosure provides for production of succinic acid from organic waste or biogas or methane using recombinant methanotrophic bacterium. In one embodiment, the recombinant methanotrophic bacterium includes exogenous nucleic acid(s) or gene(s) encoding for specified enzymes. In a further embodiment, succinic acid producing capacity of the recombinant methanotrophic bacterium is increased above the basal level by overexpression or/and downregulation of selected gene(s). In another embodiment, a process of producing succinic acid using the recombinant methanotrophic bacterium is disclosed. The present invention successfully solves the problems in converting organic waste to a useful chemical thereby providing an environment-friendly and commercially viable solution for waste management.
    Type: Grant
    Filed: April 13, 2015
    Date of Patent: February 25, 2020
    Assignee: STRING BIO PRIVATE LIMITED
    Inventor: Ezhilkani Subbian
  • Compositions comprising fungi and use thereof as a pesticide
    Patent number: 10563272
    Abstract: The present disclosure relates generally to an entomopathogenic fungi, Metarhiziuim spp, and its use to control invertebrate pests of agricultural crops, in particular, invertebrate pests which affect cotton crops.
    Type: Grant
    Filed: September 23, 2016
    Date of Patent: February 18, 2020
    Assignees: Cotton Research and Development Corporation, The Crown in right of the State of New South Wales acting through the Department of Primary Industries, as office of the NSW Department of Industry, Skills and Regional Development
    Inventor: Robert Mensah
  • Bacterium constitutively producing monophosphoryl lipid A and method of producing monophosphoryl lipid A by using bacterium
    Patent number: 10557156
    Abstract: A bacterium that constitutively produces monophosphoryl lipid A (MLA) and a method of producing MLA by using the bacterium may simply produce MLA and a derivative thereof without acid hydrolysis, reduce a probability of natural mutation, and increase yields of MLA and a derivative thereof by constitutive expression of the MLA and derivative thereof.
    Type: Grant
    Filed: July 3, 2018
    Date of Patent: February 11, 2020
    Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Hak Suk Chung, Yu Hyun Ji, Jin Su An, Ick Chan Kwon, Eun Gyeong Yang
  • Polynucleotides encoding cyclohexanone monooxygenases
    Patent number: 10557126
    Abstract: The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess.
    Type: Grant
    Filed: July 29, 2019
    Date of Patent: February 11, 2020
    Assignee: Codexis, Inc.
    Inventors: Ee Lui Ang, Oscar Alvizo, Behnaz Behrouzian, Michael D. Clay, Steven J. Collier, Ellen D. Eberhard, Fu Fan, Shiwei Song, Derek J. Smith, Magnus Widegren, Robert Wilson, Junye Xu, Jun Zhu
  • Methods of detecting a polypeptide having anaplastic lymphoma kinase activity in kidney cancer
    Patent number: 10551383
    Abstract: The invention provides methods to identify, diagnose, and treat kidney cancer through the detection of expression and/or activity of anaplastic lymphoma kinase (ALK). The detection of the presence of a polypeptide with ALK kinase activity (e.g., by detecting expression and/or activity of the polypeptide), identify those kidney cancers that are likely to respond to an ALK-inhibiting therapeutic.
    Type: Grant
    Filed: September 3, 2015
    Date of Patent: February 4, 2020
    Assignee: CELL SIGNALING TECHNOLOGY, INC.
    Inventors: Herbert Haack, Katherine Eleanor Crosby, Victoria McGuinness Rimkunas, Matthew Ren Silver
  • Modified transposases for improved insertion sequence bias and increased DNA input tolerance
    Patent number: 10544403
    Abstract: Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.
    Type: Grant
    Filed: April 29, 2019
    Date of Patent: January 28, 2020
    Assignee: ILLUMINA, INC.
    Inventors: Christian Gloeckner, Amirali Kia, Erin Bomati, Molly He, Haiying Li Grunenwald, Scott Kuersten, Trina Faye Osothprarop, Darin Haskins, Joshua Burgess, Anupama Khanna, Daniel Schlingman, Ramesh Vaidyanathan
  • Microbial ergothioneine biosynthesis
    Patent number: 10544437
    Abstract: Disclosed are methods for ergothioneine biosynthesis. More particularly, the present disclosure relates to methods for microbial ergothioneine biosynthesis. The present disclosure relates generally to engineered host cells and methods for producing ergothioneine. More particularly, the present disclosure relates to an engineered host cell and methods for microbial ergothioneine biosynthesis using the engineered host cell.
    Type: Grant
    Filed: April 28, 2015
    Date of Patent: January 28, 2020
    Assignee: Conagen Inc.
    Inventors: Jixiang Han, Hui Chen, Xiaodan Yu
  • Method of reducing serine for asparagine misincorporation
    Patent number: 10526631
    Abstract: The present invention relates to a method of reducing serine for asparagine misincorporation in a protein produced by a culture of cells in a cell culture method, the method comprising the addition of asparagine and iron to the cell culture medium.
    Type: Grant
    Filed: March 13, 2017
    Date of Patent: January 7, 2020
    Assignee: ARES TRADING S.A.
    Inventors: Thomas Solacroup, Matthieu Stettler, Hervé Broly
  • Method of using split inteins, conjugates to generate protein conjugates
    Patent number: 10526401
    Abstract: Disclosed herein are split inteins, fused proteins of split inteins, and methods of using split inteins to efficiently purify and modify proteins of interest.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: January 7, 2020
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Tom W. Muir, Miquel Vila-Perello, Zhihua Liu, Neel H. Shah
  • Nicotinamide phosphoribosyltransferase (NAMPT) mutant and use thereof
    Patent number: 10519429
    Abstract: The present invention discloses a Nicotinamide phosphoribosyltransferase (nampt) mutant and use thereof. The present invention relates to a nicotinamide phosphoribosyltransferase (Nampt) mutant artificially obtained through genic site-directed mutation. An object of the present invention is to provide a Nampt mutant having a catalytic activity higher than that of a conventional wild type parent, wherein the enzymatic activity of the Nampt mutant provided in the present invention is 1.2-6.9 times of the enzymatic activity of the parent.
    Type: Grant
    Filed: November 22, 2018
    Date of Patent: December 31, 2019
    Assignee: BONTAC BIO-ENGINEERING (SHENZHEN) CO., LTD
    Inventors: Rongzhao Fu, Qi Zhang
  • Cells containing CRISPR hybrid DNA/RNA polynucleotides
    Patent number: 10519468
    Abstract: The present disclosure provides DNA-guided CRISPR systems; polynucleotides comprising DNA, RNA and mixtures thereof for use with CRISPR systems; and methods of use involving such polynucleotides and DNA-guided CRISPR systems.
    Type: Grant
    Filed: December 18, 2017
    Date of Patent: December 31, 2019
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Andrew Paul May, Paul Daniel Donohoue
  • Phage insensitive
    Patent number: 10519519
    Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.
    Type: Grant
    Filed: July 2, 2018
    Date of Patent: December 31, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Roelof Hendrik Matthijs Kouwen, Pim Van Hee, Douwe Van Sinderen, Brian McDonnell, Jennifer Mahony
  • Modified host cells having tolerance to ?-olefins
    Patent number: 10513713
    Abstract: The present invention provides methods for improving microbial tolerance to alpha olefin compounds, host cells having increased tolerance to such compounds, and method of using the host cells to produce alpha olefin compounds.
    Type: Grant
    Filed: April 29, 2015
    Date of Patent: December 24, 2019
    Assignees: The Regents of the University of California, Total Marketing Services
    Inventors: Aindrila Mukhopadhyay, Florence Mingardon, Angelique Chanal