Patents Examined by Delia M. Ramirez
  • Engineered nucleic-acid targeting nucleic acids
    Patent number: 10501728
    Abstract: The present disclosure provides engineered cross-type-nucleic-acid targeting nucleic acids and compositions thereof. Nucleic acid sequences encoding the engineered cross-type-nucleic-acid targeting nucleic acids, as well as expression cassettes, vectors and cells comprising such nucleic acid sequences, are described. Also, methods are disclosed for making and using the engineered cross-type-nucleic-acid targeting nucleic acids and compositions thereof.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: December 10, 2019
    Assignee: Caribou Biosciences, Inc.
    Inventors: Paul Daniel Donohoue, Andrew Paul May
  • Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
    Patent number: 10494656
    Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Grant
    Filed: April 22, 2019
    Date of Patent: December 3, 2019
    Assignee: Codexis, Inc.
    Inventors: Haibin Chen, Steven J. Collier, Jovana Nazor, Joly Sukumaran, Derek Smith, Jeffrey C. Moore, Gregory Hughes, Jacob Janey, Gjalt W. Huisman, Scott J. Novick, Nicholas J. Agard, Oscar Alvizo, Gregory A. Cope, Wan Lin Yeo, Stefanie Ng Minor
  • strains with amplified expression of cellulases and production of biofuel therefrom
    Patent number: 10464974
    Abstract: Transgenic strains of Neurospora crassa engineered to comprise a synthetic positive feedback loop for a transcriptional activator of cellulase expression such that cellulase production is amplified are disclosed, along with compositions thereof. The transgenic strains are particularly useful in methods for generating purified cellulases, fermentable sugars, and cellulosic ethanol for the efficient production of biofuel from cellulose-containing biomass and waste.
    Type: Grant
    Filed: January 8, 2016
    Date of Patent: November 5, 2019
    Assignee: University of Cincinnati
    Inventors: Christian Hong, Toru Matsuura
  • Integrated system for programmable DNA methylation
    Patent number: 10465187
    Abstract: Provided herein are engineered systems and methods for establishing DNA adenine methylation at specific genomic locations and using DNA adenine methylation as an artificial chemical “handle” on the genome. These systems and methods allow for placing the handle on specific genomic locations as well as molecular technologies to bind, spatially spread, and maintain the handle. The systems described herein comprise, in some embodiments, three functional modules that mediate m6A operations: (1) a synthetic initiator module to place m6A at specific genomic sites; (2) a synthetic readout module to program m6A recognition and m6A-dependent transcriptional logic; and (3) propagation module that implements “read-write,” a mechanism proposed to underlie chromatin spreading and epigenetic maintenance across cellular systems.
    Type: Grant
    Filed: February 6, 2018
    Date of Patent: November 5, 2019
    Assignee: Trustees of Boston University
    Inventors: Ahmad S. Khalil, Albert J. Keung, Minhee Park
  • Artificial forisome body with SEO-F fusion proteins, plant or yeast cells comprising vectors which code for these proteins, as well as vectors which code for SEO-F fusion proteins
    Patent number: 10442840
    Abstract: The invention relates to artificial forisome bodies including a fusion protein of at least one SEO-F protein or an at least 50-amino acid portion thereof, and at least one additional protein or peptide, with the exception of GFP and the Venus protein, wherein in an embodiment the forisome body further includes an unfused SEO-F protein or a form of the protein having C-terminal deletions of up to 45 amino acids and/or N-terminal deletions of up to 13 amino acids, wherein the unfused SEO-F protein is selected from proteins having the property of being capable of forming homomeric forisome bodies in the absence of additional SEO-F proteins.
    Type: Grant
    Filed: May 2, 2013
    Date of Patent: October 15, 2019
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der Angewandten Forchung e.V.
    Inventors: Boje Mueller, Dirk Pruefer, Rainer Fischer
  • Adipate (ester or thioester) synthesis
    Patent number: 10435723
    Abstract: The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid (6-ACA), a method for preparing 5-formyl valeric acid (5-FVA), and a method for preparing caprolactam. Further, the invention relates to a host cell for use in a method according to the invention.
    Type: Grant
    Filed: April 27, 2016
    Date of Patent: October 8, 2019
    Assignee: Genomatica, Inc.
    Inventors: Liang Wu, Axel Christoph Trefzer, Stefaan Marie Andre De Wildeman, Marco Alexander Van Den Berg
  • Preparation of (R)-3-hydroxybutyric acid or its salts by one-step fermentation
    Patent number: 10428357
    Abstract: The subject invention relates to a process of preparing (R)-3-hydroxybutyric acid or a salt thereof by one-step fermentation with a nonpathogenic microorganism. The fermentation of (R)-3-hydroxybutyric acid was performed by supplying with certain carbon and nitrogen sources. These microorganisms include a Glutamic acid Bacterium HR057 strain or one type of genetically engineered Corynebacterium Glutamicum.
    Type: Grant
    Filed: April 3, 2018
    Date of Patent: October 1, 2019
    Assignee: NNB Nutrition USA, LLC
    Inventors: Kylin Liao, Wenchao Fan
  • Production of arabitol
    Patent number: 10428355
    Abstract: A method for producing arabitol may include providing a fermentation culture having a microorganism and a carbon source; allowing the microorganism to ferment the carbon source; monitoring a process condition of said step of allowing the microorganism to ferment the carbon source; and collecting a product from the fermentation culture after said step of monitoring a process condition indicates that a predetermined change in the process condition has occurred. Other methods may include steps of providing soybean-based lignocellulosic hydrolysate as a carbon source for a fermentation culture, and modifying the pH of one or more of the growth phase and the stationary phase of a fermentation process.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: October 1, 2019
    Assignee: THE UNIVERSITY OF AKRON
    Inventors: Lu-Kwang Ju, Abdullah Al Loman
  • Modified polymerases for improved incorporation of nucleotide analogues
    Patent number: 10421996
    Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogs, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: September 24, 2019
    Assignee: ILLUMINA, INC.
    Inventors: Erin Bomati, Molly He, Cheng-Yao Chen, Sergio Peisajovich
  • Microorganism for production of putrescine and methods for production of putrescine using the same
    Patent number: 10415068
    Abstract: Provided are a novel modified ornithine decarboxylase protein having improved putrescine productivity and a use thereof.
    Type: Grant
    Filed: November 2, 2018
    Date of Patent: September 17, 2019
    Assignees: Korea Advanced Institute of Science and Technology, CJ Cheiljedang Corporation
    Inventors: Hyang Choi, Hee Kyoung Jung, Hak Sung Kim, Hyun Ho Kyeong, Jung Min Choi, Joong Jae Lee, Hyo Deok Seo
  • Biocatalysts and methods for the synthesis of armodafinil
    Patent number: 10400223
    Abstract: The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess.
    Type: Grant
    Filed: August 27, 2018
    Date of Patent: September 3, 2019
    Assignee: Codexis, Inc.
    Inventors: Ee Lui Ang, Oscar Alvizo, Behnaz Behrouzian, Michael D. Clay, Steven J. Collier, Ellen D. Eberhard, Fu Fan, Shiwei Song, Derek J. Smith, Magnus Widegren, Robert Wilson, Junye Xu, Jun Zhu
  • Method for producing L-amino acids using a bacterium of the family enterobacteriaceae overexpressing a gene encoding an iron exporter
    Patent number: 10392638
    Abstract: The present invention provides a method for producing L-amino acids by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress a gene encoding an iron exporter, such as a fetB gene, fetA gene, fieF gene, or a combination of these genes.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: August 27, 2019
    Assignee: AJINOMOTO CO., INC.
    Inventors: Anastasia Yurievna Romkina, Alexey Nikolaevich Chernyshov, Yulia Georgievna Rostova, Elvira Borisovna Voroshilova, Mikhail Yurievich Kiryukhin
  • Pregnancy-associated plasma protein-A2 (PAPP-A2) polypeptides
    Patent number: 10392610
    Abstract: The present invention provides pregnancy associated plasma protein A2 (PAPP-A2), its nucleotide and amino acid sequences, antisense molecules to the nucleotide sequences which encode PAPP-A2, expression vectors for the production of purified PAPP-A2, antibodies capable of binding specifically to PAPP-A2, hybridization probes or oligonucleotides for the detection of PAPP-A2-encoding nucleotide sequences, genetically engineered host cells for the expression of PAPP-A2, and methods for screening for pathologies in pregnant and non-pregnant patients. Methods for screening for altered focal proliferation states in pregnant and/or non-pregnant patients, which include detecting levels of PAPP-A2, are also described.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: August 27, 2019
    Assignee: Como Biotech ApS
    Inventors: Claus Oxvig, Michael Toft Overgaard
  • Modified transposases for improved insertion sequence bias and increased DNA input tolerance
    Patent number: 10385323
    Abstract: Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: August 20, 2019
    Assignee: ILLUMINA, INC.
    Inventors: Christian Gloeckner, Amirali Kia, Erin Bomati, Molly He, Haiying Li Grunenwald, Scott Kuersten, Trina Faye Osothprarop, Darin Haskins, Joshua Burgess, Anupama Khanna, Daniel Schlingman, Ramesh Vaidyanathan
  • strains
    Patent number: 10385309
    Abstract: The present invention relates to a method of preparing a strain of sugar fermenting Saccharomyces cerevisiae with capability to ferment xylose, wherein said method comprises different procedural steps. The method comprises mating a first sporulated Saccharomyces cerevisiae strain with a second Saccharomyces cerevisiae haploid strain. Thereafter, screening for mated cells is performed, growing such mated cells, and verifying that mated cells exhibit basic morphology by microscopic inspection. Thereafter, creation of a mixture of the mated cells is performed, subjecting the mixture to continuous chemostat lignocellulose cultivation and obtaining the sugar fermenting Saccharomyces cerevisiae cells with capability to ferment xylose is performed. The invention also comprises strains obtained by said method.
    Type: Grant
    Filed: April 23, 2015
    Date of Patent: August 20, 2019
    Assignee: SCANDINAVIAN TECHNOLOGY GROUP AB
    Inventor: Nicklas Bonander
  • Subtilase variants and compositions comprising same
    Patent number: 10378001
    Abstract: The present invention relates to novel subtilase variants exhibiting increased stability and optionally on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.
    Type: Grant
    Filed: June 27, 2014
    Date of Patent: August 13, 2019
    Assignee: Novozymes A/S
    Inventors: Jens Erik Nielsen, Pernille Ollendorf Micheelsen, Jurgen Carsten Franz Knotzel, Maria Norman Hockauf, Lars Beier, Michael Bauer, Annette Helle Johansen, Lars Lehmann Christensen, Julie Billie Rannes
  • Method for producing L-?-amino acid compound
    Patent number: 10364448
    Abstract: Isolated oxidases, isolated polynucleotides encoding the oxidases, and methods of using the oxidases to produce ?-oxocarboxylic acid compounds or L-?-amino acid compounds are described.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: July 30, 2019
    Assignee: Sumitomo Chemical Company, Limited
    Inventor: Hiroyuki Asako
  • Mutant-type glucose dehydrogenase and use thereof
    Patent number: 10351892
    Abstract: The present invention provides a mutant-type glucose dehydrogenase having glucose dehydrogenase activity and having decreased reactivity with xylose, wherein said mutant-type glucose dehydrogenase comprises a mutant-type ?-subunit comprising an amino acid sequence of 520 to 550 amino acids comprising an amino acid sequence of 520 to 550 amino acids comprising SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24 and SEQ ID NO: 25 in this order from N-terminus to C-terminus, except that one or more amino acid residue(s) selected from the group consisting of the glycine at position 10 in SEQ ID NO: 23, the histidine at position 4 in SEQ ID NO: 24, and the asparagine at position 4 in SEQ ID NO: 25 is/are substituted with another/other amino acid(s).
    Type: Grant
    Filed: October 28, 2016
    Date of Patent: July 16, 2019
    Assignees: ARKRAY, Inc., Ultizyme International Ltd.
    Inventors: Katsuhiro Kojima, Kazushige Mori
  • Recombinase mutants
    Patent number: 10344269
    Abstract: Presented herein are recombinases for improved recombinase-mediated amplification of nucleic acids, such as a PCR-library having single-stranded adapter regions, on a patterned flow cell surface for improved cluster amplification, as well as methods and kits using the same.
    Type: Grant
    Filed: May 11, 2018
    Date of Patent: July 9, 2019
    Assignee: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Erin Bomati, Matthew William Kellinger, Jonathan Mark Boutell
  • Reversible regulation of intein activity through engineered new zinc binding domain
    Patent number: 10323235
    Abstract: Disclosed are compositions comprising an engineered intein designed such that the self-cleaving activity of the intein can be modulated by a zinc-binding motif as well as methods and systems for making and using the compositions.
    Type: Grant
    Filed: September 18, 2017
    Date of Patent: June 18, 2019
    Assignee: Ohio State Innovation Foundation
    Inventors: Buyong Ma, David F. Nellis, Jianwei S. Zhu, David W. Wood