Patents Examined by Delia M. Ramirez
  • Variant amylase enzyme compositions and methods
    Patent number: 10808234
    Abstract: The invention is directed to novel amylases.
    Type: Grant
    Filed: May 2, 2019
    Date of Patent: October 20, 2020
    Assignee: Fornia BioSolutions, Inc.
    Inventors: Xiyun Zhang, Jie Yang, Khin Oo, Goutami Banerjee, Tatsuya Fukushima, Eric Lin Hu, Marielle De Jesus Palatino, Jijiao Zeng, Imad N. Sawaya
  • Engineered meganucleases with recognition sequences found in the human T cell receptor alpha constant region gene
    Patent number: 10799535
    Abstract: Disclosed herein are recombinant meganucleases engineered to recognize and cleave a recognition sequence present in the human T cell receptor alpha constant region gene. The present disclosure further relates to the use of such recombinant meganucleases in methods for producing genetically-modified eukaryotic cells.
    Type: Grant
    Filed: October 5, 2016
    Date of Patent: October 13, 2020
    Assignee: Precision BioSciences, Inc.
    Inventors: Michael G. Nicholson, James Jefferson Smith, Derek Jantz, Victor Bartsevich, Daniel T. MacLeod, Jeyaraj Antony
  • Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
    Patent number: 10787689
    Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Grant
    Filed: October 17, 2019
    Date of Patent: September 29, 2020
    Assignee: Codexis, Inc.
    Inventors: Haibin Chen, Steven J. Collier, Jovana Nazor, Joly Sukumaran, Derek Smith, Jeffrey C. Moore, Gregory Hughes, Jacob Janey, Gjalt W. Huisman, Scott J. Novick, Nicholas J. Agard, Oscar Alvizo, Gregory A. Cope, Wan Lin Yeo, Stefanie Ng Minor
  • Biocatalysts and methods for the synthesis of armodafinil
    Patent number: 10781429
    Abstract: The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess.
    Type: Grant
    Filed: December 16, 2019
    Date of Patent: September 22, 2020
    Assignee: Codexis, Inc.
    Inventors: Ee Lui Ang, Oscar Alvizo, Behnaz Behrouzian, Michael D. Clay, Steven J. Collier, Ellen D. Eberhard, Fu Fan, Shiwei Song, Derek J. Smith, Magnus Widegren, Robert Wilson, Junye Xu, Jun Zhu
  • Method of preparing a strain of and a method of fermentation to produce ethanol
    Patent number: 10767155
    Abstract: The present invention relates to a method of preparing a strain of sugar fermenting Saccharomyces cerevisiae with capability to ferment xylose, wherein said method comprises different procedural steps. The method comprises mating a first sporulated Saccharomyces cerevisiae strain with a second Saccharomyces cerevisiae haploid strain. Thereafter, screening for mated cells is performed, growing such mated cells, and verifying that mated cells exhibit basic morphology by microscopic inspection. Thereafter, creation of a mixture of the mated cells is performed, subjecting the mixture to continuous chemostat lignocellulose cultivation and obtaining the sugar fermenting Saccharomyces cerevisiae cells with capability to ferment xylose is performed. The invention also comprises strains obtained by said method.
    Type: Grant
    Filed: July 3, 2019
    Date of Patent: September 8, 2020
    Assignee: SCANDINAVIAN TECHNOLOGY GROUP AB
    Inventor: Nicklas Bonander
  • GF423 strain, and composition for providing antioxidant and anti-inflammatory activities or preventing or treating hyperlipidemia, including polypeptide produced by the same
    Patent number: 10751393
    Abstract: The present invention relates to a Bacillus amyloliquefaciens strain that produces superoxide dismutase (SOD), and also relates to an antioxidant, anti-inflammatory pharmaceutical composition and a pharmaceutical composition and food composition for preventing or treating hyperlipidemia, which include a superoxide dismutase produced by the Bacillus amyloliquefaciens strain. The compositions of the present invention exhibit excellent effects without causing side effects, and may thus be used as functional raw materials or products having an enhanced activity of preventing or treating inflammation, cancer or hyperlipidemia in the pharmaceutical drug, food, cosmetic and livestock fields.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: August 25, 2020
    Assignee: GENOFOCUS INC.
    Inventors: Do Young Yum, Jeong Hyun Kim, Jae Gu Pan, Eui Joong Kim, Taek Ho Yang, Ji Eun Kang, Soo Young Park, Hyun Do Kim
  • Heme protein catalysts for carbon-boron bond formation in vitro and in vivo
    Patent number: 10745673
    Abstract: Provided herein are methods for producing an organoboron product. The methods include combining a boron-containing reagent and a carbene precursor in the presence of a heme protein, e.g., a cytochrome c, a cytochrome P450, a globin, a protoglobin, a nitric oxide dioxygenase, a peroxidase, or a catalase, or a variant thereof, under conditions sufficient to form the organoboron product. Reaction mixtures for producing organoboron products are also described, as well as whole-cell catalysts comprising heme proteins and variants thereof for forming carbon-boron bonds.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: August 18, 2020
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: S. B. Jennifer Kan, Xiongyi Huang, Kai Chen
  • Modified polymerases for improved incorporation of nucleotide analogues
    Patent number: 10745751
    Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.
    Type: Grant
    Filed: August 15, 2019
    Date of Patent: August 18, 2020
    Assignee: ILLUMINA, INC.
    Inventors: Erin Bomati, Molly He, Cheng-Yao Chen, Sergio Peisajovich
  • Production of itaconic acid and related molecules from aromatic compounds
    Patent number: 10738333
    Abstract: This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that produces itaconate or trans-aconitate. The disclosure further provides methods for producing itaconate or trans-aconitate using a genetically-modified bacterium from the genus Pseudomonas.
    Type: Grant
    Filed: April 29, 2019
    Date of Patent: August 11, 2020
    Assignees: UT-BATTELLE, LLC, ALLIANCE FOR SUSTAINABLE ENERGY, LLC
    Inventors: Joshua R. Elmore, Jay Huenemann, Davinia Salvachua, Gregg T. Beckham, Adam M. Guss
  • Transformant for producing 4-hydroxybenzoic acid or salt thereof
    Patent number: 10738296
    Abstract: The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or mere other amino acids.
    Type: Grant
    Filed: January 10, 2019
    Date of Patent: August 11, 2020
    Assignee: GREEN CHEMICALS CO., LTD.
    Inventors: Masayuki Inui, Kazumi Hiraga, Masako Suda, Ryoma Hashimoto
  • Enzyme exhibiting fructan hydrolase activity
    Patent number: 10716308
    Abstract: The present invention is related to an enzyme that allows efficient removal of fructan from grain and vegetable raw material. The enzyme according to the invention produces grain and vegetable material having a fructan content significantly lower compared to that of the starting material.
    Type: Grant
    Filed: June 22, 2017
    Date of Patent: July 21, 2020
    Assignee: Oy Karl Fazer Ab
    Inventors: Jussi Loponen, Markku Mikola, Juhani Sibakov
  • Methods of mobilizing soil phosphorus with a synergistic bacterial consortia
    Patent number: 10710938
    Abstract: The present disclosure relates to consortia of bacteria strains and composition comprising one or more bacterial strains disclosed herein. These consortia of isolated bacteria cultures and compositions comprising said cultures, having greater activity than would be observed for the individual bacteria cultures and compositions. A composition of the disclosure may advantageously be used for enhancing the availability of soil phosphorus and other macronutrients and/or micronutrients to plants, and thereby enhancing their growth and yield.
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: July 14, 2020
    Assignee: COLORADO STATE UNIVERSITY RESEARCH FOUNDATION
    Inventors: Matthew D. Wallenstein, Colin W. Bell
  • Engineered nucleic-acid targeting nucleic acids
    Patent number: 10711258
    Abstract: The present disclosure provides engineered cross-type-nucleic-acid targeting nucleic acids and compositions thereof. Nucleic acid sequences encoding the engineered cross-type-nucleic-acid targeting nucleic acids, as well as expression cassettes, vectors and cells comprising such nucleic acid sequences, are described. Also, methods are disclosed for making and using the engineered cross-type-nucleic-acid targeting nucleic acids and compositions thereof.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: July 14, 2020
    Assignee: Caribou Biosciences, Inc.
    Inventors: Paul Daniel Donohoue, Andrew Paul May
  • Recombinant engineered bacterium co-expressing trans-anethole oxygenase and formate dehydrogenase and application thereof in production of piperonal
    Patent number: 10711291
    Abstract: The present application discloses a method for producing piperonal by using a recombinant engineered bacterium co-expressing trans-anethole oxygenase and formate dehydrogenase, and an engineered bacterium thereof, including constructing a formate dehydrogenase gene fdh and trans-anethole oxygenase gene tao or trans-anethole oxygenase mutant gene co-expression recombinant vector; inductively expressing recombinant genetically engineered bacterium; and producing piperonal by using the recombinant genetically engineered bacterium. 15.91 g/L of piperonal with a transformation rate of 79.55% and a time-space transformation rate of 2.27 g/L/h can be finally obtained during catalysis, and the yield is significantly improved compared with the existing piperonal, thereby being more conducive to the smooth realization of industrial production.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: July 14, 2020
    Assignee: Jiangnan University
    Inventors: Pu Zheng, Dan Wu, Peng Wen, Pengcheng Chen, Yin Fu
  • Alpha-amylase variant with altered properties
    Patent number: 10612012
    Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
    Type: Grant
    Filed: April 1, 2019
    Date of Patent: April 7, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus von der Osten
  • Microorganisms and methods for the production of aniline
    Patent number: 10612029
    Abstract: A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline.
    Type: Grant
    Filed: November 19, 2018
    Date of Patent: April 7, 2020
    Assignee: GENOMATICA, INC.
    Inventor: Priti Pharkya
  • Use of an esterase to enhance ethyl ester content in fermentation media
    Patent number: 10604776
    Abstract: Methods to prepare vegetable oil compositions having an elevated ethyl ester content are provided.
    Type: Grant
    Filed: May 24, 2018
    Date of Patent: March 31, 2020
    Assignee: Poet Research, Inc.
    Inventors: Alexander T. McCurdy, Steven T. Bly, Stephen M. Lewis, Benjamin P. Gacke, Brandon James Breitling
  • Method of producing proteins in filamentous fungi with decreased CLR1 activity
    Patent number: 10597429
    Abstract: The present invention relates to a method of producing a recombinant polypeptide in a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 1 (CLR1) and to express the recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR1 and to the use of this filamentous fungus in the production of a recombinant polypeptide.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: March 24, 2020
    Assignee: BASF SE
    Inventors: Stefan Haefner, Andreas Thywissen, Holger Hartmann, Nico Boehmer
  • Enzyme scaffolds and methods of use
    Patent number: 10577633
    Abstract: Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: March 3, 2020
    Assignee: Alliance for Sustainable Energy, LLC
    Inventors: Yannick J. Bomble, Michael E. Himmel, Jeffrey Linger, Roman Brunecky, John Aikens
  • Compositions and methods for protein glycosylation
    Patent number: 10577592
    Abstract: Described herein are oligosaccharyl transferases for use in N-glycosylating proteins of interest in vitro and in host cells. Methods for using such oligosaccharyl transferases, nucleic acids encoding such oligosaccharyl transferases, and host cells comprising such oligosaccharyl transferases are also provided herein. Glycoconjugates generated by using such oligosaccharyl transferases are also provided herein.
    Type: Grant
    Filed: November 1, 2018
    Date of Patent: March 3, 2020
    Assignee: GlaxoSmithKline Biologicals SA
    Inventors: Jurgen Haas, Julian Ihssen, Michael Thomas Kowarik, Torsten Franz Schwede, Linda Christiane Thöny-Meyer