Abstract: Expression constructs are provided employing a plant EF-1.alpha. promote which allows for elevated expression in rapidly dividing cells. Sequences from the gene and untranslated regions associated with the gene may be employed in an antisense construct to reduce growth rate. The promoter finds particular use in protecting rapidly dividing tissue and tender shoots from a wide variety of environmentally induced stress conditions.

Abstract: The present invention provides a method for producing plants with labdane production in Nicotiana which comprises subjecting plans to somaclonal variation, and screening for plants having higher than average labdane content. The invention also provides improved Nicotiana sp. plant lines producing at least about 800 mg of cis-abienol or sclareol per kg of fresh plant weight.

Abstract: Transgenic plants have been created which express an insect-specific toxin from a scorpion. The chimeric inheritable trait produced conditions of toxicity in the plant cells of toxicity to certain insects upon ingestion of plant tissues. The inheritable trait has also been cross-bred to plants transgenic to the Bacillus thuringiensis delta-endotoxin to produce plants having two independent insect-specific toxin traits. Insect feeding trails revealed additive toxic effects. A generalized approach for developing other insecticidal toxins as candidates for insertion into transgenic plants is also presented.

Abstract: A transgenic mouse with enhanced viral resistance transmissible to its offspring is prepared by introduction of a gene encoding a human interferon having antiviral activity into a host mouse. The gene encodes the interferon of a different animal species and therefore its expression product is less toxic to the host at an embryonic, fetal, neonatal or junvenile stage of development. Preferably, the human beta interferon gene is introduced into the cells of a mouse.

Abstract: A male transgenic mouse containing germ cells and somatic cells which contain a recombinant gene which is a vertebrate gene in the int-2/FGF family which is capable of promoting benign prostatic hyperplasia or hypertrophy in said transgenic mouse, said gene being introduced into the mouse, or an ancestor of the mouse, at an embryonic stage.

Abstract: Transgenic mice having a phenotype characterized by the substantial absence of mature T lymphocytes otherwise naturally occurring in said mice. The phenotype is conferred in the transgenic mouse by a transgene contained in at least the precursor stem cell of the T lymphocyte which is depleted. The transgene comprises a DNA sequence encoding a T cell receptor polypeptide variant which inhibits maturation of the T lymphocyte.

Abstract: Methods are provided for determining the oncogenic potential of chemical compounds which utilizes a transgenic mouse predisposed to T-cell lymphomas. The transgenic mouse expresses a pim-1 oncogene and as a consequence is predisposed to the spontaneous onset of T-cell lymphomas. The oncogenic potential of a chemical compound is determined by administering a known dose of the chemical compound of interest to a pim-1 transgenic mouse. Thereafter, the transgenic mouse is monitored to detect the onset of a T-cell lymphoma. The time of onset of the T-cell lymphoma and the dosage of the chemical compound are compared to either the onset of spontaneous T-cell lymphomas in the pim-1 transgenic mouse or to the onset of a T-cell lymphoma in a pim-1 transgenic mouse which has been exposed to a known quantity of a carcinogenic agent. This provides an indication of the oncogenic potential of the chemical compound.

Abstract: Novel transcription initiation regions that provide for enhanced transcription of a DNA sequence, particularly a plant sequence, are provided.

Abstract: The invention relates to a novel method for transforming and rapidly regenerating plant tissue. The method employs target tissues which are the shoot apices thereby expanding the species range for transformation and reducing the risk of somaclonal variation.

Abstract: The present invention relates to a recombinant multigene comprising a plurality of structural genes and a plurality of DNA sequences which encode peptide linkers. In the present invention, one of the structural genes encodes a protease, the DNA sequences encoding the peptide linkers are adjacent to the DNA sequences which encode the structural genes and the peptide linkers contain an amino acid sequence which the protease recognizes as a proteolytic cleavage site. The present invention additionally relates to transgenic plants which contain such a recombinant multigene transgene, host cells transformed with such a recombinant multigene, and transgenic animals which contain such a recombinant multigene transgene. Furthermore, the present invention relates to a method of producing a plurality of polypeptides in a host by incorporating and expressing in the host a such recombinant multigene.

Abstract: A method for introducing a replication-defective retroviral vector into pluripotent stem cells of embryos of an avian species, including chickens, turkeys, quails or ducks. The method is useful for transferring nucleic acid sequences into embryonic avian cells which may differentiate into somatic or germ cells. Transfer into germ cells has been achieved to produce transgenic animals. The replication-defective retroviral vector used for transfer may be a recombinant retroviral vector containing both a retroviral derived nucleic acid sequence and a non-retroviral derived nucleic acid sequence. Examples of non-retroviral nucleic acid sequences are a neomycin resistance gene from the bacterial transposon Tn5, a herpes simplex virus thymidine kinase gene and a chicken growth hormone gene, however, any prokaryotic or eukaryotic nucleic acid sequence of interest may be used. Transgenic chickens have been produced whose cells contain and express a replication-defective retroviral vector nucleic acid sequence.

Abstract: A panel of monoclonal antibody (Mab) producing hybridomas directed against various antigens of Bordetella pertussis are disclosed herein. The hybridomas and the antigens that the resulting monoclonals are directed against include: (1) BPG10F8C3 and BPE8D8B1--lipooligosaccharide A (LOS A), serotype 1; (2) BPD5, BPE6, and BPF2--fimbriae, serotype 2; (3) BPE3, BPE8 and BPD8--69 kDa nonfimbrial protein, serotype 3; and (4) BPB7, BPC10, BPD4, BPD6, BPD9, and BPF5--fimbriae, serotype 6.

Abstract: The present invention provides a maize plant wherein the growth and development of said plant is tolerant of inhibition by a cyclohexanedione herbicide, an aryloxyphenoxypropanoic acid herbicide, or mixtures thereof, at levels which normally inhibit the growth and development of maize, wherein said tolerance is conferred by an altered acetyl coenzyme A carboxylase (ACCase) tolerant of inhibition by said herbicide at levels which normally inhibit the activity of an unaltered ACCase.

Abstract: According to the invention, there is provided an inbred corn line, designated PHP55. This invention thus relates to the plants and seeds of inbred corn line PHP55 and to methods for producing a corn plant produced by crossing the inbred line PHP55 with itself or with another corn plant. This invention further relates to hybrid corn seeds and plants produced by crossing the inbred line PHP55 with another corn line or plant and to crosses with related species.

Abstract: According to the invention, there is provided an inbred corn line, designated PHV37. This invention thus relates to the plants and seeds of inbred corn line PHV37 and to methods for producing a corn plant produced by crossing the inbred line PHV37 with itself or with another corn plant. This invention further relates to hybrid corn seeds and plants produced by crossing the inbred line PHV37 with another corn line or plant and to crosses with related species.

Abstract: An improved process for the economical production of F.sub.1 hybrid Oryza sativa plants (i.e., hybrid rice plants of the first filial generation) is provided. Novel female fertile male sterile rice plants which are perennial and capable of retaining viability through the winter are planted in combination with annual female fertile male fertile restorer plants which are killed during the winter. Accordingly, the substantial expense of establishing the required male sterile plants at the beginning of each growing season effectively is eliminated. The seeds formed on the perennial male sterile plants are capable of forming annual male fertile F.sub.1 hybrid Oryza sativa plants. In preferred embodiments additional properties are incorporated into the parent rice plants (as described) which further enhance the F.sub.1 seed production.

Abstract: According to the invention, there is provided an inbred corn line, designated PHR63. This invention thus relates to the plants and seeds of inbred corn line PHR63 and to methods for producing a corn plant produced by crossing the inbred line PHR63 with itself or with another corn plant. This invention further relates to hybrid corn seeds and plants produced by crossing the inbred line PHR63 with another corn line or plant and to crosses with related species.

Abstract: A method for the production of a plant of reduced stature is provided comprising the introduction of a bacterial cell into a seed or a plant, the bacterial cell being capable of replicating in the plant and of inducing a reduction in plant stature. A seed and a plant modified by introduction of such a bacterial cell are also provided.

Abstract: According to the invention, there is provided an inbred corn line, designated PHN73. This invention thus relates to the plants and seeds of inbred corn line PHN73 and to methods for producing a corn plant produced by crossing the inbred line PHN73 with itself or with another corn plant. This invention further relates to hybrid corn seeds and plants produced by crossing the inbred line PHN73 with another corn line or plant and to crosses with related species.

Abstract: According to the invention, there is provided an inbred corn line, designated PHN82. This invention thus relates to the plants and seeds of inbred corn line PHN82 and to methods for producing a corn plant produced by crossing the inbred line PHN82 with itself or with another corn plant. This invention further relates to hybrid corn seeds and plants produced by crossing the inbred line PHN82 with another corn line or plant and to crosses with related species.