Abstract: Novel methods for stable in vitro propagation of human hepatitis virus using non-human hepatocytes are disclosed. Also discussed are novel systems for efficient expression and secretion of foreign genes employing particular rat hepatoma cells.

Abstract: New type II restriction endonuclease MamI has recognition sequence: ##STR1## and a cleavage site indicated by the arrows. It is preferably obtained from microorganisms of the genus Microbacterium.

Abstract: The present invention is related to the promoter of the gene for the human precursor of the Alzheimer's Disease A4 amyloid protein (PAD gene).Another object of the present invention is the use of this promoter in a method of establishing a screening model for the Alzheimer's Disease. Thus the present invention is also related to a vector useful in the transfection of cells and the cells and animals transfected therewith.

Abstract: Process for introducing foreign DNA into the genome of plants by infecting plants or explantates thereof or plant protoplasts or by incubating plant protoplasts with Agrobacterium, in which Agrobacteria are used, which contain one or more T-regions and a Vir-region from the Ti-plasmid of Agrobacterium in their genetic material and/or one or more T-regions comprising an artificial T-region which consists of each DNA desired, flanked by border sequences as present in the wild type T-region of Agrobacterium or sequences being functionally analogous thereto.

Abstract: A plant cell which is resistant to a herbicidal glutamine synthetase inhibitor, wherein the resistance is caused by levels of GS activity which, when present in an otherwise herbicidal GS inhibitor sensitive plant cell, render the cell substantially resistant to the herbicidal GS-inhibitor.

Abstract: A new and innovative technique of producing Lilium elegans Asiatic hybrid lily is disclosed. Very small bulbils from leaf axils of L. elegans receives sequential low-high-low temperature treatment before planting in the soil medium. After planting, scale leaves emerge and bulb weight increases. After about 13 scale leaves have emerged from the bulbs, the temperature is lowered to favor shoot emergence, preferably under long day photoperiod to accelerate shoot emergence and to induce flower bud formation. A desirable plant producing up to three flowers on a long stem is produced in about 270-300 days after harvesting bulbils, thus bypassing the field production phase of the bulb that lasts more than a year in the field.

Abstract: We have identified a new human gene, SCL. We discovered this gene because of its involvement in a chromosomal translocation associated with the occurrence of a stem cell leukemia manifesting myeloid and lymphoid differentiation capabilities. Here we report the sequence of a cDNA for the normal SCL transcript, as well as for an aberrant fusion transcript produced in the leukemic cells. Although different at their 3' untranslated regions, both cDNAs predict a protein with primary amino acid sequence homology to the previously described amphipathic helix-loop-helix DNA binding and dimerization motif of the Lyl-1, myc, MyoD, Ig enhancer binding, daughterless, and achaete-scute families of genes. For these cDNAs, two forms of the SCL protein (greater than 20 and 30 kD) are predicted, both of which retain this putative DNA binding domain. The pattern of expression of SCL mRNA is primarily predominant in early hematopoietic tissues.

Abstract: A tough mutant strain of the bioluminescent marine dinoflagellate pyrocys lunula which produces almost three times more light than its predecessor Pyrocystis lunula strain, which can withstand much more centrifugal force than its predecessor, and which grows faster than its predecessor in a defined medium under laboratory conditions, doubling about every four days, and requiring only monthly transfers.

Abstract: This invention relates to new stable sulpho-adenosyl-L-methionine (SAMe) salts and the relative production process.Said salts have the following general formula:SAMe.nR(O).sub.m (SO.sub.3 H).sub.p (I)where m can be zero or 1; n is 1.5 when p is 2, and is 3 when p is 1; R is chosen from the group consisting of alkyl, phenylalkyl and carboxyalkyl, in which the linear or branched alkyl chain contains from 8 to 18 carbon atoms.In particular, the salts according to the present invention are SAMe salts of sulphonic acids, or of sulphuric acid esters, or of dioctylsulphosuccinic acid, which fall within formula (I).The process for producing said salts consists of: a) enriching the starting yeast with SAMe; b) lysing the cells and recovering an aqueous solution rich in SAMe (cell lysate); c) purifying the lysate by ultrafiltration; d) precipitating the SAMe by treatment with one of the aforesaid acids or esters; e) separating the precipitated product, washing it and drying it under vacuum.

Abstract: A stable mutant human megakaryocytic cell line is disclosed which is useful for the study of megakaryocytopoiesis, platelet formation and production, platelet component formation, and the identification and characterization of factors which modulate megakaryocytopoiesis.

Abstract: Novel compositions are provided for modulating growth, particularly of tumor cells, which compositions are combinations of Oncostatin M, and one or both of transforming growth factors or .gamma.-interferons, or analogs thereof. In addition, a novel transforming growth factor is provided, designated TGF-.beta.2, as well as methods for its preparation.

Abstract: The methods of the invention can be used to identify sites of cleavage of an enzyme in the presence of hypochlorite. It has been found that such cleavage occurs at tryptophan. Chemical modifications or genetic manipulation to change or delete tryptophan can be done to produce a more stable enzyme which retains activity in the presence of hypochlorite. The invention is particularly applicable to alkaline proteases which are useful in detergent compositions.

Abstract: The present invention relates to a method for inducing male sterility in plants which comprises treating seeds of a plant receptive to said treatment by application of an effective amount of a compound of the formula: ##STR1## wherein R and R.sup.1 are hydrogen, alkyl, alkenl, alkynl, allyl, aryl, halogen or boron and may be the same or different;X and X.sup.1 are fluorine, chlorine, bromine or iodine; nitrate, sulfate, sulfonate, phosphate, citrate, or maleate;n and n.sup.1 are the integers 0 or 1, and may be the same or different;m and m.sup.1 are integers from 0 to 4, and may be the same or different;for a period of time and under conditions sufficient to induce male sterility in adult plants which are grown from said treated seeds.

Abstract: Nucleic acids encoding the .beta. chain of the human granulocyte-macrophage colony stimulating factor (GM-CSF) receptor, as well as the .beta. chain itself, are provided. The .beta. chain may be expressed with the .alpha. chain in cellular hosts to form compositions useful in screening agonists and antagonists of human GM-CSF.

Abstract: Permanent infection of a cell line such as a canine thymus cell line with a retrovirus such as equine infectious anemia virus and bovine Maedi-Visna-like virus is now possible. By culturing such an infected cell line under appropriate conditions, it is now possible to produce large quantities of viral antigens on a continuous basis. Such antigens are useful in for diagnostics and research.

Abstract: Fragments of the 35S promoter of cauliflower mosaic virus are disclosed which exhibit selective expression of chimeric plant genes in plant tissue. Promoter fragment A exhibits selective expression in root tissue and the radical of the seed. Promoter fragment B exhibits constitutive expression in plant tissue other than root tissue.

Abstract: A cell culturing system, methods for the preparation thereof, and methods for affixing other biologically active moieties to a substrate are provided. Said cell culturing system comprises: a substrate; a coating thereon of a sterile formulation comprising polyphenolic protein containing from about 35 to 100% by weight pure bioadhesive polyphenolic protein having the repeating decapeptide unit: ##STR1##wherein N is a whole number ranging from about 10 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; viable cells affixed to said coated substrate; and a nutritive medium contacting said cells, whereby said cells perform normal metabolic cell functions.

Abstract: Regulation of expression of genes encoded for in plant cell genomes is achieved by integration of a gene under the transcriptional control of a promoter which is functional in the host and in which the transcribed strand of DNA is complementary to the strand of DNA that is transcribed from the endogenous gene(s) one wishes to regulate. The integrated gene, referred to as anti-sense, provides an RNA sequence capable of binding to naturally existing RNAs, exemplified by polygalacturonase, and inhibiting their expression, where the anti-sense sequence may bind to the coding, non-coding, or both, portions of the RNA. The antisense construction may be introduced into the plant cells in a variety of ways and be integrated into the plant genome for inducible or constitutive transcription of the anti-sense sequence. A wide variety of plant cell properties may be modifed by employing this technique.The pCGN978xK12 was deposited at the A.T.C.C. on Mar. 25, 1986, and given Accession No.

Abstract: This invention relates to a novel dwarf Brassica oleracea plant and a useful variety of rapid-cycling B. oleracea containing the single recessive mutant gene for the dwarf phenotype homozygously. The single gene (dwfl) is produced by mutagenesis and selection and is useful for producing novel types and varieties of dwarf Brassica plants for ornamental or agricultural purposes.

Abstract: A transgenic avian line, which is resistant to infection by avian leukosis virus (ALV), subgroup A, has been produced. The line was discovered as a chance event occurring in a program of infecting embryonic chickens with a recombinant ALV virus, selecting individuals assayed to be positive for genomic insertion of proviral sequences, and backcrossing to the original line to obtain G1 progeny demonstrating stable inheritance of the inserts. Of at least three proviral inserts defective for ALV production, one was found to be capable of expressing in the cell membrane subgroup A envelope glycoprotein which specifically interferes with infection by subgroup A virus. This insert represents an artificially introduced dominant gene for ALV disease resistance. Through an appropriate breeding program, a line of chickens homozygous for this gene has been obtained.