Abstract: A method of growing anchorage-dependent cells is disclosed in which a substrate comprising a hydrogel is employed. The hydrogel is formed from an aqueous solution gelled with a crosslinked polymer of a hydrophilic monomer and includes a macromolecule capable of supporting cell growth.

Abstract: Purified BMP-5 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.

Abstract: A high titer fermentation process using transformed Escherichia coli cells carrying a plasmid containing DNA which codes for Somatomedin C is disclosed. Titers of about 900-1000 mg/L have been obtained using the method of the present invention; about two times the titer of prior art processes.

Abstract: The invention provides genetically modified plant cells having a plant structural gene introduced and expressed therein under the control of a T-DNA promoter. In particular, the invention provides genetically modified plant cells having the phaseolin gene introduced and expressed therein under the control of a T-DNA promoter. Also provided are novel strains of bacteria containing and replicating T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter. Further, the invention provides plasmids having the ability to replicate in E. coli and comprising T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter.

Abstract: The present invention relates to new stable sulpho-adenosyl-L-methionine (SAMe) salts particulary suitable for parenteral use, their production process, and pharmaceutical compositions containing them as active principles.Said salts correspond to the general formula:SAMe.n(CH.sub.2).sub.m (SO.sub.3 H).sub.2 (I)where n can vary from 1 to 2 and m can vary from 3 to 12.The process for producing said salts consists of the following stages: a) enriching the starting yeast with SAMe; b) lysing the cells and recovering a solution rich in SAMe (cell lysate); c) prepurifying the cell lysate by ultrafiltration; d) passing the prepurified lysate through a column of weak acid ion exchange resin and eluting with the required disulphonic acid; e) passing the eluate of said column through a colum of absorption resin and washing with the required disulphonic acid; f) concentrating the eluate of the later column by reverse osmosis; g) drying the concentrated solution.

Abstract: Mature human serum albumin is produced from a human serum albumin produced by a microbiological route in the form of fused protein ("pseudo-pro-HSA") containing an N-terminal peptide elongation.

Abstract: The present invention is directed to a method for cloning and producing the AseI restriction endonuclease by 1) introducing the restriction endonuclease gene from A. serpens ATCC 12638 into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the AseI restriction endonuclease, and 3) purifying the AseI restriction endonuclease from the fermented host which contains the vector encoding and expressing the AseI restriction endonuclease activity.

Abstract: According to the invention, there is provided an inbred corn line, designated PHM10. This invention thus relates to the plants and seeds of inbred corn line PHM10 and to methods for producing a corn plant produced by crossing the inbred line PHM10 with itself or with another corn plant. This invention further relates to hybrid corn seeds and plants produced by crossing the inbred line PHM10 with another corn line or plant and to crosses with related species.

Abstract: Five subdomains of the CaMV35S promoter are provided that cause tissue specific and/or developmentally regulated expression of chimeric genes in plants. These subdomains act as promoters for use in transformed plant cells, seeds and transgenic plants. Some of the subdomains require fusion to domain A for expression. Subdomains B2, B3, B4, and B5 exhibit expression when fused to the minimal promoter sequence in mature plants whereas only B2 and B3 confer expression in seeds and only B2, B3 and B4 confer expression at the seedling stage of development. The combination of subdomains B4 and B5 confers expression at all stages of development as does the B1+TGACG motif combination when each combination is fused with the minimal promoter sequence. The nucleotide sequence and DNA molecule that function as the enhancers are provided.

Abstract: According to the invention, there is provided an inbred corn line, designated PHP60. This invention thus relates to the plants and seeds of inbred corn line PHP60 and to methods for producing a corn plant produced by crossing the inbred line PHP60 with itself or with another corn plant. This invention further relates to hybrid corn seeds and plants produced by crossing the inbred line PHP60 with another corn line or plant and to crosses with related species.

Abstract: This invention relates generally to the field of cell culture. The invention presents pH based methods for controlling the glycosylation patterns of cell products, particularly proteins produced by cells in vitro, preferably via a multi-level pH control strategy. Preferably, this invention is applied to manage the effect of high level of ammonia on the glycosylation of cell products. The glycosylation affected is preferably that of terminal sialylations of the oligosaccharide of glycoproteins.

Abstract: The invention concerns novel isolates of Bacillus thuringiensis (B.t.) which contains a toxin(s) which is active against adult nematode worms and larvae. This B.t. toxin(s) can be used to treat animals and plants hosting susceptible nematodes.

Abstract: The present invention relates to a continuous cell-line tamarin adenocarcinoma of the colon derived from the species Saquinus oedipus oedipus which is designated TAC-1. The invention is also directed to a method for establishing an adenocarcinoma cell-line, which comprises, obtaining a sample of a colorectal tumor, and then mincing, incubating and subculturing the sample. Finally, the invention is directed to a method for the serial passage of the cell-line and a suitable medium therefor.

Abstract: Highly concentrated hematopoietic stem cell compositions are provided which are substantially free of differentiated or dedicated hematopoietic cells. The cells are obtained by subtraction of cells having particular markers and selection of cells having particular markers. The resulting composition may be used to provide for individual or groups of hematopoietic lineages, to reconstitute stem cells of the host, and to identify an assay for a wide variety of hematopoietic growth factors.

Abstract: A transgenic non-human eukaryotic animal whose germ cells and somatic cells contain an activated oncogene sequence introduced into the animal, or an ancestor of the animal, at an embryonic stage.

Abstract: Novel constructs are provided containing DNA fragments comprising a Rhizobium nodulation gene divergent transcriptional initiation regulatory region. The region is responsive to plant exudate in the presence of a nod D gene product. When associated with plants, the region can control the expression of structural genes, such as agents active in protecting plants or inducing their growth.This invention was funded at least in-part by a grant from the National Institutes of Health. The U.S. government may have certain rights in this invention.

Abstract: The nucleotide sequence of the promoter region from pollen-specific genes of corn is disclosed. The sequence consists of at least 1315 base pairs upstream from a region of DNA which hybridizes to mRNA found only in pollen. In addition, a sequence of DNA containing over 500 base pairs downstream from the transcribed region has also been cloned and sequenced. The sequenced regions can be used to introduce into plants genes, the expression of which in pollen is desired. A suicide gene can be added to the 3' end of the promoter to induce abortion of pollen development and thereby product male sterile plants. Other genes coding for insect toxins or nutritional proteins can be expressed in pollen by use of the pollen-specific promoter.

Abstract: A method for producing a stigma-like tissue of a saffron flower comprising (1) culturing a corolla tube of a saffron in the presence of a plant hormone or (2) culturing a female organ and/or a corolla tube of a saffron flower in the presence of a plant hormone and alanine, and a method for producing useful components of a saffron flower, e.g., saffron pigment, from the thus produced stigma-like tissue. The tissue cultures produce larger quantitites of the stigma-like tissue and useful components, compared to that obtained by conventional tissue cultures.

Abstract: The utilization of a positive selection seed screening process to isolate rate variants with resistance to a herbicide is described. The ability to screen large numbers of mutagenized seed has made it feasible to pursue and isolate plants with low frequency dominant herbicide-resistance mutations. The dominant herbicide resistance mutations are useful for many applications including expanding the utility of sulfonylurea herbicides for soybean weed control, production of F.sub.1 and F.sub.2 seeds, and as selectable markers for efficient seed purification.

Abstract: An inbred corn plant containing dominant genetic information that expresses high protein and increased lysine levels in corn kernels produced by the plant is provided. The genetic information in the plant can be used to produce novel inbred corn lines and hybrid corn lines containing high protein and lysine levels both by established plant breeding techniques and other techniques of genetic manipulation.