Abstract: Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one amide compound selected from the group consisting of acrylamide, methacrylamide, crotonamide, and n-butyramide in the preparation of cells of bacteria having nitrile hydratase activity by cultivating Pseudomonas bacteria capable of producing nitrile hydratase.

Abstract: A novel strain Trichosporon Kashiwayama, a culture product or culture liquid of said strain, sterile liquid, sterile filtrate, sterile supernatant of said culture liquid, or concentrate or dry product thereof, processes for preparations thereof, a dermatic medicament having curative effect of skin disorder or a cosmetic consisting solely of or comprising as a main ingredient the above product, liquid, filtrate, supernatant, concentrate or dry product.

Abstract: A mutant Bacillus subtilis, arising from spontaneous adaptation from a parent without detectable aspartase activity, produces recoverable amounts of aspartase. The micro-organism attains maximum growth and enzyme production within about 8 hours and shows excellent glucose tolerance. Aspartase is at least in part produced constitutively, but L-aspartic acid stimulates further aspartase production. The aspartase so produced is readily released from the cell upon cell wall rupture and shows adequate extra-cellular stability for further concentration and purification.

Abstract: A novel antitumor agent designated herein as rebeccamycin is produced by fermentation of Nocardia aerocolonigenes (ATCC 39243). Rebeccamycin and its 5'-N-methyl and 5',2",3",6"-tetraacetate derivatives exhibit activity against experimental animal tumor systems.

Abstract: The present invention relates to a method for separating lysozyme from egg-white which has a lysozyme fraction and an albumin fraction, without destroying the commercial utility of the lysozyme fraction or the albumin fraction. The method comprises the step of preferentially, reversibly binding the lysozyme fraction of the egg-white to an affinity resin at about the natural pH of the egg-white by contacting the egg-white with the resin, separating the albumin fraction from the resin to leave the lysozyme fraction bound to the resin in a lysozyme-resin complex, and eluting the bound lysozyme from the resin to obtain a lysozyme solution and a reusable resin. The eluted lysozyme and the separated albumin fraction are further treated in separating processing lines to produce a commercial albumin fraction egg-white product and a commercial lysozyme product. The invention also deals with the preparation and compositions of suitable affinity resins for the reversible, preferential binding of lysozyme in egg-white.

Abstract: A strain of Paracoccus denitrificans, NRRL B-15710, is an overproducer of a fumarase which is readily purified and relatively thermostable. The fumarase so produced converts fumaric acid to L-malic acid without formation of any significant amounts of a coproduct.

Abstract: A mutant Streptomyces thermoviolaceus, NRRL 15615, elaborates a thermostable glucose isomerase in high yield, constitutively. The GI produced shows a negligible loss in activity when heated at 90.degree. C. in high fructose corn syrup for 5 minutes relative to the same treatment at 80.degree. C. The mutant microorganism produces about 1500 units of glucose isomerase per gram of dry weight cells in the absence of xylose.

Abstract: A process for the preparation of a plasminogen activator is described, which comprises bringing normal diploid cells derived from human bodies capable of producing the plasminogen activator into contact with a solution containing an enzymatically-decomposed animal meat peptone, the plasminogen activator having the following properties:(a) molecular weight: 63,000.+-.10,000;(b) isoelectric point: 7.0 to 8.5;(c) affinity to fibrin: present;(d) affinity to Concanavalin A: present;(e) optimum pH: 7 to 9.5; and(f) no reactivity with anti-urokinase specific antibody.The plasminogen activator can be obtained in a high yield.

Abstract: An aqueous enzyme preparation stabilized with an ester of the formula RCOOR' where R is an alkyl of from one to three carbons or hydrogen and R' is an alkyl of from one to six carbons, the ester being in an amount of from 0.1 to about 2.5% by weight.

Abstract: This invention relates to a process for producing a TF-500 substance having carcinostatic and immunostimulating activities, which comprises subjecting organisms belonging to the Fusobacterium genus to extraction treatment with a dialkyl sulfoxide and obtaining said TF-500 substance from the resulting extract.

Abstract: Leucine dehydrogenase is disclosed which retains 80% or more of the activity even after it is incubated in a buffer solution at about 70.degree. C. for 10 minutes, as compared to the activity prior to incubation. A process for producing a heat resistant leucine dehydrogenase is also disclosed. The process comprises culturing a microorganism having an optimum growth temperature of 50.degree. to 85.degree. C. and harvesting the leucine dehydrogenase from the culture and a composition of assaying for leucine aminopeptidase (LAP) comprising the heat resistant leucine dehydrogenase.The leucine dehydrogenase can be stored for a long period of time and thus very effective for investigations in biochemistry, food analysis and clinical test. Further the composition provides extremely easy assay for the LAP activity in high accuracy with good reproducibility which is an important item in clinical test.

Abstract: A process for producing carbohydrates from vegetal juice comprises extracting a vegetal juice from raw vegetal material, filtering the vegetal juice to remove material suspended therein, subjecting the resultant vegetal juice to an enzymatic reaction which consists of four stages in the following sequence. The vegetal juice in a first stage is admixed with a mixture of 3.2.1.4-.beta.-1,4-glucan glucanhydrolase and 3.2.1.15-poly-.alpha.-1,4-galacturonic glucanhydrolase, then the vegetal juice is admixed with 3.2.1.20-.alpha.-D-glucoside glucohydrolase. In the second stage the vegetal juice is admixed with 3.2.1.20-.alpha.-D-glucoside glucohydrolase; in the third state the vegetal juice is contacted with 3.2.1.21-.beta.-D-glucoside glucohydrolase, 5.3.1.4-L-arabinose-ketol-isomerase and 5.3.1.5-D-xylose-ketol-isomerase; and in the fourth stage the vegetal juice is admixed with 3.2.1.1-.alpha.-1,4-glucan-4-glucanhydrolase.

Abstract: Novel odor-flavor materials and methods for producing the same are disclosed comprising a mixture of various gamma-lactones. In the preferred embodiment, a member of the genus Pityrosporum is cultured on a culture medium and is stimulated to produce a gamma-lactone containing flavor-fragrance mixture through its incubation with a lipid rich precursor. The preferred species for use in producing these gamma-lactone containing materials are P. canis, P. pachydermatis, P. orbiculare and P. ovale. The preferred lipid-rich materials include triolein, sebum, lecithin, oleic acid, and SAB with human sebum, and Tween-80. The resulting gamma-lactone rich products comprise the following lactones: gamma-hexa, gamma-hepta, gamma-octa, gamma-nona, gamma-deca, gamma-undeca, and in the case of P. canis, gamma-dodecalactone. The resultant flavor-fragrance product is all natural, exhibiting extremely pleasing taste and/or flavor characteristics.

Abstract: This invention relates to a method for manufacturing restriction enzymes, which comprises cultivating a restriction enzyme-producing strain belonging to Bifidobacterium and recovering the restriction enzyme from the bacteria thus cultivated.

Abstract: A process for producing glucose dehydrogenase consists of cultivating a mutant strain (ATCC 39 118) of Bacillus megaterium in a culture medium and recovering the resultant glucose dehydrogenase. The latter can be used in the enzymatic production of L-carnitine consisting of subjecting 3-dehydrocarnitine to the simultaneous action of carnitine dehydrogenase, nicotinamide adenine dinucleotide and glucose and glucose dehydrogenase.

Abstract: Antibiotic U-66,026 is produced in a fermentation under controlled conditions using the microorganism Alcaligenes sp., NRRL B-15269. Enhanced fermentation of titers U-66,026 are obtained when Alcaligenes sp., NRRL B-15269, is cultivated in mixture with Streptomyces plicatus strain 395, NRRL 15273.Antibiotic U-66,026 is a useful antibiotic which has antifungal activity.

Abstract: Starch liquefaction is accomplished using fungal amylase at a temperature of from about 75.degree. C. to 80.degree. C.

Abstract: A new alcohol oxidase is isolated from Pichia-type microorganisms in soluble or crystalline form. The crystalline novel enzyme is isolated by preparing an aqueous fluid containing cells of a Pichia-type microorganism, homogenizing the fluid and separating solids therefrom to produce an alcohol oxidase solution, adjusting the solution to have an ionic strength in the range of 0.05 to 0.01 in ionic strength to form a recovery range solution thereby causing the crystalline alcohol oxidase to form. The new enzyme is used to determine alcohol concentrations in fluid samples in which conditions are compatible with the enzyme's activity.

Abstract: From the fermentation carried, out with mutants blocked in the synthesis respectively of erythromycin and of oleandomycin, namely Streptomyces erythreus ATCC 31772 and Streptomyces antibioticus ATCC 31771, using as the substrate a derivative of erythronolide A, namely (8S)-B-fluoroerythronolide A, a derivative of erythronolide B, namely (8S)-B-fluoroerythronolide B, or a derivative of 3-O-mycarosyl-erythronolide B, namely 3-O-mycarosyl-(8S)-fluoroerythronolide B, the corresponding (8S)-8-fluoro derivatives of the erythromycins A,B, C and D, as well as 3-O-oleandrosyl-5-desosaminyl-(8S)-8-fluoroerythronolide A and 3-O-oleandrosyl-5-O-desosaminyl-(8S)-8-fluoroerythronolide B, all belonging to the class of the marcolide antibiotics are obtained.

Abstract: A method of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of a reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.