Patents Examined by Lionel M. Shapiro
  • Restriction endonuclease XcyI
    Patent number: 4588689
    Abstract: A novel restriction endonuclease designated XcyI recognizes and cleaves the sequence 5'-C.dwnarw.CCGGG-3', where the arrow indicates the cleavage site. The enzyme may be obtained from Xanthamonas cyanopsidis.Xanthamonas cyanopsidis strain 13D5 was deposited at the American Type Culture Collection on Jan. 20, 1984, and granted accession No. 39587.
    Type: Grant
    Filed: March 2, 1984
    Date of Patent: May 13, 1986
    Assignee: The Regents of the University of California
    Inventors: Clarence I. Kado, Raymond L. Rodriguez, Byron E. Froman, Robert C. Tait
  • Microbiological oxidation reactions using purified monooxygenase enzyme components
    Patent number: 4587216
    Abstract: A purified hydroxylase enzyme component A of the methane monooxygenase enzyme isolated from the soluble fraction of Methylobacterium organophilum (CRL.26) (NRRL B-11,222) is found to contain three subunits. Any component A derived from methylotrophs having the particular characteristics of this isolated component A may be employed in conjunction with the flavoprotein component C of the methane monooxygenase enzyme, preferably the flavoportein component derived from the same organism, to catalyze the oxidation of various oxidizable organic substrates to their respective oxidation products. Preferably, the substrate is propylene.
    Type: Grant
    Filed: August 31, 1983
    Date of Patent: May 6, 1986
    Assignee: Exxon Research and Engineering Co.
    Inventors: Ramesh N. Patel, Leonard E. Mortenson
  • Highly thermostable amyloglucosidase
    Patent number: 4587215
    Abstract: Microorganisms of the species Talaromyces thermophilus elaborate an amyloglucosidase which shows remarkable thermostability. The enzyme shows biphasic decay, where an initial decay, corresponding to loss of 25-40% activity, is followed by essentially no further loss in activity at 70.degree. C. under conditions where a prior art thermostable amyloglucosidase showed a half-life of 53 minutes.
    Type: Grant
    Filed: June 25, 1984
    Date of Patent: May 6, 1986
    Assignee: UOP Inc.
    Inventor: Jody K. Hirsh
  • Method for the production of phenylalanine ammonia-lyase by fermentation
    Patent number: 4584273
    Abstract: A method for the production of the enzyme, phenylalanine ammonia-lyase (PAL), by fermentation, wherein the stability and useful life of the enzyme are improved by maintaining PAL-containing media in substantially anaerobic, static conditions. PAL is useful for catalyzing the conversion of t-cinnamic acid and ammonia to L-phenylalanine.
    Type: Grant
    Filed: October 31, 1983
    Date of Patent: April 22, 1986
    Assignee: Genex Corporation
    Inventors: Malcolm A. J. Finkelman, Huei-Hsuing Yang
  • Adenylate kinase and process for the production thereof
    Patent number: 4584272
    Abstract: A heat-resistant adenylate kinase is described whose activity after an incubation in a buffer solution at about 50.degree. C. for about 15 minutes is at least about 80% of the original activity prior to the incubation. This adenylate kinase can be obtained by cultivating a bacterium belonging to the genus Bacillus and collecting adenylate kinase from the resulting culture broth. This heat-resistant enzyme is very stable against heat and, therefore, after isolation, it can be stored for a long period of time compared with the conventional adenylate kinase.
    Type: Grant
    Filed: October 7, 1981
    Date of Patent: April 22, 1986
    Assignees: Unitika Ltd., Rikagaku Kenkyusho, Kazutomo Imahori
    Inventors: Kazutomo Imahori, Hiroshi Nakajima, Kazuhiko Nagata
  • Method for stabilizing the enzymatic activity of phenylalanine ammonia lyase during L-phenylalanine production
    Patent number: 4584269
    Abstract: A method for the enzymatic conversion of t-cinnamic acid and ammonia to L-phenylalanine, using the catalyst, phenylalanine ammonia-lyase, wherein the stability and useful life of the enzyme are improved by conducting the conversion under substantially anaerobic, static conditions.
    Type: Grant
    Filed: October 31, 1983
    Date of Patent: April 22, 1986
    Assignee: Genex Corporation
    Inventors: Patricia J. Vollmer, Jeffrey J. Schruben, John P. Montgomery, Huei-Hsuing Yang
  • Purification of phenylethanolamine N-methyltransferase
    Patent number: 4582806
    Abstract: The present invention provides an efficient process for purifying the enzyme phenylethanolamine N-methyltransferase suitable for use in radioenzymatic assays of endogenous compounds.
    Type: Grant
    Filed: July 2, 1984
    Date of Patent: April 15, 1986
    Assignee: Eli Lilly and Company
    Inventors: Ronald R. Bowsher, David P. Henry
  • Staged immobilized amyloglucosidase and immobilized glucose isomerase in producing fructose from thinned starch
    Patent number: 4582803
    Abstract: The limitation of an immobilized amyloglucosidase in hydrolyzing thinned starch to afford not more than about 93% glucose with isomaltose levels above about 1.5% can be overcome in a process for converting thinned starch to fructose using four closely coupled reactor stages. The first stage is a saccharification reactor using amyloglucosidase which converts thinned starch to a product containing from 50% to 85% glucose. This product is used in a first stage isomerization reactor, the effluent from which is sent to another saccharification reactor using immobilized amyloglucosidase where hydrolysis is continued until no more than about 6% disaccharides and higher oligosaccharides are present. Where this effluent is used as a feedstock for further conversion of glucose to fructose, it is operationally equivalent to a feedstock containing at least 94% glucose but with isomaltose levels under about 1.5%.
    Type: Grant
    Filed: June 11, 1984
    Date of Patent: April 15, 1986
    Assignee: UOP Inc.
    Inventors: H. Peter G. Knapik, William H. Mueller
  • Erythromycin D and esters thereof by fermentation with Nocardia sp. ATCC 39043
    Patent number: 4582796
    Abstract: Fermentation of a culture of Nocardia sp. ATCC 39043 produces an antibiotic complex comprising erythromycin D, 3",4"-di-O-acetylerythromycin D, 3"-O-acetyl-4"-O-propionylerythromycin D and 4"-O-acetylerythromycin D. The components are separated and are each useful in vitro and in vivo as antibacterial agents. If erythromycin D is the desired product, the esters can be hydrolyzed prior to the separation of the erythromycin D.
    Type: Grant
    Filed: April 16, 1984
    Date of Patent: April 15, 1986
    Assignee: Pfizer, Inc.
    Inventors: Walter D. Celmer, Walter P. Cullen, Paul C. Watts, Riichiro Shibakawa, Junsuke Tone
  • Control of bean rust with Bacillus subtilis
    Patent number: 4582704
    Abstract: A strain of Bacillus subtilis designated APPL-1 and a purified extract of APPL-1 were found to effectively control and inhibit rust on bean plants.
    Type: Grant
    Filed: October 7, 1983
    Date of Patent: April 15, 1986
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventors: Con J. Baker, J. Rennie Stavely
  • Novel alkaline protease
    Patent number: 4581332
    Abstract: A novel protease is described. The enzyme has the following characteristics: molecular weight: 30,000 (a gel filtration method using Sephadex G-75); isoelectric point, 5.3; pH reactivity: the optimum pH for its esterase activity is at pH 8.5 and the optimum pH for its amidase activity is at pH 9.0 respectively; substrate reactivity: it selectively and specifically hydrolyzes the ester bond and the amide bond derived from the carboxyl group of L-lysine; inhibitor: it is inhibited by diisopropyl phosphofluoride, tosyl-L-lysine chloro-methyl ketone and phenylmethylsulfonyl chloride. The protease is preferably produced by a microorganism belonging to the genus Achromobacter.
    Type: Grant
    Filed: September 28, 1983
    Date of Patent: April 8, 1986
    Assignee: Wako Pure Chemical Industries, Ltd.
    Inventors: Masami Soejima, Takeharu Masaki, Hideya Suzuki
  • Process for producing dihydroxyacetone kinase
    Patent number: 4579822
    Abstract: A process for producing dihydroxyacetone kinase comprises the cultivation of the DHAK-producing strains of genus Schizosaccharomyces, and the purification of dihydroxyacetone kinase from the culture.
    Type: Grant
    Filed: April 10, 1984
    Date of Patent: April 1, 1986
    Assignee: Amano Pharmaceutical Co., Ltd.
    Inventors: Nobuya Itoh, Kuniyoshi Matsunaga
  • Novel thermostable, aciduric alpha-amylase and method for its production
    Patent number: 4578352
    Abstract: This invention relates to an alpha-amylase enzyme exhibiting thermostability at an acidic pH which is derived from a spore-forming, thermophilic, anaerobic bacterium and to a process for its production. This alpha-amylase is especially useful for the preparation of glucose-containing syrups from starch.
    Type: Grant
    Filed: July 13, 1983
    Date of Patent: March 25, 1986
    Assignee: CPC International Inc.
    Inventors: Dennis M. Katkocin, Nancy S. Word, Shiow-Shong Yang
  • Method for enhancing a fungus-lytic activity of .beta.-1,3-D-glucanase
    Patent number: 4576914
    Abstract: A method of enhancing a fungus-lytic activity of .beta.-1,3-D-glucanase which comprises using said glucanase in the presence of one or more of the activators selected from the group consisting of sodium lauroylsarcosinate, polyoxyethylene alkylphenyl ether, polyoxyethylene alkyl ether, polyoxyethylene polyoxypropylenealkyl ether, benzalkonium chloride, ammonium chloride, chlorhexidine glucuronate, methylparaben, propylparaben, trypsin, Pronase.RTM. and Alcalase.RTM..A method of enhancing a fungus-lytic activity of .beta.-1,3-D-glucanase which comprises using two .beta.-1,3-D-glucanases of different origins is also provided.
    Type: Grant
    Filed: October 17, 1983
    Date of Patent: March 18, 1986
    Assignee: Rohto Pharmaceutical Co., Ltd.
    Inventors: Akiyoshi Yoshida, Shigeru Kametaka, Shin'ichi Hayashi
  • Stabilization of phenylalanine ammonia-lyase in a bioreactor using reducing agents
    Patent number: 4574117
    Abstract: A method is disclosed for producing L-phenylalanine using, as a catalyst, phenylalanine ammonia-lyase (PAL). Reducing agents are added to a bioreaction mixture containing t-cinnamic acid and ammonia or soluble ammonium salts, in order to reduce the effects of oxygen on catalyst life. The reducing agents of the invention include any substance that lowers the overall electrochemical potential of the solution, or which has a tendency to donate electrons to an oxidizing agent.
    Type: Grant
    Filed: June 6, 1984
    Date of Patent: March 4, 1986
    Assignee: Genex Corporation
    Inventors: Patricia J. Vollmer, Jeffrey J. Schruben
  • Human leukemia virus-related peptides, a process for production thereof, antibodies of the peptides and a process for production of the antibodies
    Patent number: 4572800
    Abstract: An antibody of a human leukemia virus-related peptide obtained by collecting an antibody produced in a mammal body by administering to the mammal an antigen prepared by reacting a human leukemia virus-related peptide represented by formula (1):R-Pro-Val-Met-His-Pro-His-Gly-Ala-Pro-OH (1)whereinR is a hydrogen atom or a group shown by formula, H-Tyr-;as a hapten, with a carrier in the presence of a hapten-carrier binding agent.
    Type: Grant
    Filed: September 23, 1983
    Date of Patent: February 25, 1986
    Assignee: Otsuka Pharmaceutical Co., Ltd.
    Inventors: Fumio Shimizu, Tetsuya Tachikawa, Nobuhiro Ikei, Atunari Noda, Etsuro Hashimura, Kenichi Imagawa
  • Thermophile isolate having thermostable hydrolytic activity
    Patent number: 4572898
    Abstract: This application represents an invention based upon the discovery of a novel thermophilic organism, its isolation from natural source and to the unique thermostable hydrolytic activity produced by this bacterium, this activity being useful for industrial purposes.
    Type: Grant
    Filed: December 14, 1982
    Date of Patent: February 25, 1986
    Assignee: Genentech, Inc.
    Inventor: Joseph G. Zeikus
  • Production of bilirubin oxidase
    Patent number: 4569912
    Abstract: Method for producing bilirubin oxidase by cultivating Schizophyllum commune K-17.
    Type: Grant
    Filed: January 11, 1984
    Date of Patent: February 11, 1986
    Assignee: Takara Shuzo Co., Ltd.
    Inventors: Susumu Matsui, Takako T. Sato, Kazuo Nakajima
  • Method of making aspartic acid and purifying aspartase
    Patent number: 4569911
    Abstract: The aspartase from a mutant Bacillus subtilis, NRRL B-15536, is produced in relatively high cell yield within a comparatively short time. The enzyme converts fumaric acid to L-aspartic acid stoichiometrically with outstanding selectivity and productivity. The enzyme is stabilized by divalent magnesium ions, 2-mercaptoethanol, and ammonium furmarate, and can be conveniently purified with high recovery.
    Type: Grant
    Filed: January 19, 1984
    Date of Patent: February 11, 1986
    Assignee: UOP Inc.
    Inventor: Yoshihisa Tsuda
  • Process for preparing uridine diphosphate-N-acetylgalactosamine
    Patent number: 4569909
    Abstract: A process for preparing uridine diphosphate-N-acetylgalactosamine, which comprises treating a reaction solution obtained by the enzymatic conversion of uridine diphosphate-N-acetylglucosamine to uridine diphosphate-N-acetylgalactosamine, with uridine diphosphate-N-acetylglucosamine pyrophosphorylase to decompose the remaining uridine diphosphate-N-acetylglucosamine in the solution and then separating therefrom the uridine diphosphate-N-acetylgalactosamine for purification. In one aspect of this invention, it relates to a method for measuring the activity of .alpha.-N-acetylgalactosaminyl transferase characterized by the use of said reaction solution as the substrate for the transferase.
    Type: Grant
    Filed: June 1, 1983
    Date of Patent: February 11, 1986
    Assignee: Seitetsu Kagaku Co., Ltd.
    Inventors: Taiko Seno, Yasuto Okubo, Masao Kawamura, Seiichi Akutsu, Hirosuke Fukuda