Patents Examined by Lionel M. Shapiro
  • Maltogenic amylase enzyme, preparation and use thereof
    Patent number: 4604355
    Abstract: A novel maltogenic amylase enzyme with improved thermostability is provided. The novel enzyme can be produced by cultivating a newly discovered microorganism Bacillus strain NCIB 11837 belonging to the Bacillus stearothermophilus complex.
    Type: Grant
    Filed: March 20, 1984
    Date of Patent: August 5, 1986
    Assignee: Novo Industri A/S
    Inventor: Helle Outtrup
  • Process for preparing uridine diphosphate-N-acetylgalactosamine
    Patent number: 4604349
    Abstract: A process for preparing uridine diphosphate-N-acetylgalactosamine, which comprises treating a reaction solution obtained by the enzymatic conversion of uridine diphosphate-N-acetylglucosamine to uridine diphosphate-N-acetylgalactosamine, with uridine diphosphate-N-acetylglucosamine pyrophosphorylase to decompose the remaining uridine diphosphate-N-acetylglucosamine in the solution and then separating therefrom the uridine diphosphate-N-acetylgalactosamine for purification. In one aspect of this invention, it relates to a method for measuring the activity of .alpha.-N-acetylgalactosaminyl transferase characterized by the use of said reaction solution as the substrate for the transferase.
    Type: Grant
    Filed: February 25, 1985
    Date of Patent: August 5, 1986
    Assignee: Seitetsu Kagaku Co., Ltd.
    Inventors: Taiko Seno, Yasuto Okubo, Masao Kawamura, Seiichi Akutsu, Hirosuke Fukuda
  • Starch hydrolyzates and preparation thereof
    Patent number: 4603110
    Abstract: Starch hydrolyzates having a dextrose equivalent value of not more than about 25 which contain up to about 20% glucose by weight and up to about 40% maltose by weight, with the combination of glucose and maltose not exceeding about 40% by weight of the composition.
    Type: Grant
    Filed: October 21, 1983
    Date of Patent: July 29, 1986
    Assignee: Grain Processing Corporation
    Inventors: Alpha L. Morehouse, Pamela A. Sander
  • Coryneform bacteria carrying recombinant plasmids and their use in the fermentative production of L-threonine and L-isoleucine
    Patent number: 4601983
    Abstract: A genetic sequence coding for the production of a protein having the activity of homoserine dehydrogenase and having two Pst I cleavage sites in its DNA chain and a molecular weight of 2.24 Md, is incorporated into a vehicle capable of replication in Coryneform bacteria and used to produce L-threonine and L-isoleucine by fermentation.
    Type: Grant
    Filed: June 15, 1983
    Date of Patent: July 22, 1986
    Assignee: Ajinomoto Co., Inc.
    Inventors: Shigeru Nakamori, Masaaki Ishida, Hiroshi Takagi, Kiyoshi Miwa, Koichi Ito, Konosuke Sano
  • Novel bilirubin oxidase, its production and use
    Patent number: 4600689
    Abstract: The present invention relates to a novel bilirubin oxidase produced by a strain belonging to the genus Trachyderma of the class Basidiomycetes and its production, a reagent composition for bilirubin containing said novel bilirubin oxidase, a method for the quantitative determination of bilirubin in test solutions such as biological fluids with said reagent composition, and a method for applying said reagent composition to analytical methods in which the interfering effect of bilirubin needs to be eliminated.
    Type: Grant
    Filed: September 10, 1984
    Date of Patent: July 15, 1986
    Assignee: Takara Shuzo Co., Ltd.
    Inventors: Susumu Matsui, Yoshio Yoshihama, Tsutomu Taniguchi
  • Thermostable alpha amylase having a low requirement for calcium ions, derived from a bacillus microorganism
    Patent number: 4600693
    Abstract: The present invention relates to a novel thermostable alpha amylase having a low requirement for calcium ions, and a novel Bacillus microorganism that produces it extracellularly. This amylase is useful in high temperature, low calcium applications, e.g., in detergent compositions.
    Type: Grant
    Filed: February 13, 1984
    Date of Patent: July 15, 1986
    Assignee: Corning Glass Works
    Inventors: Karen L. Kindle, Stanley E. Mainzer, Deborah L. Marlatt, Constance B. Sawyer
  • R-(Z)-4-amino-3-chloro-2-pentenedioic acid, novel antibacterial agent
    Patent number: 4600691
    Abstract: R-(Z)-4-amino-3-chloro-2-pentenedioic acid is a novel antibacterial and isolated from Streptomyces viridogenes MA5450, ATCC 39387.
    Type: Grant
    Filed: April 3, 1985
    Date of Patent: July 15, 1986
    Assignee: Merck & Co., Inc.
    Inventors: Louis Chaiet, Richard L. Monaghan, Sheldon B. Zimmerman, Maria I. M. Fernandez
  • Process for producing antitumor antibiotic compound
    Patent number: 4599310
    Abstract: A new cyclic depsipeptide antitumor antibiotic designated herein as sandramycin is produced by fermentation of a new microorganism, Nocardioides sp. strain C49,009, ATCC 39419. Sandramycin possesses antibacterial activity and inhibits the growth of tumors in experimental animals.
    Type: Grant
    Filed: September 25, 1985
    Date of Patent: July 8, 1986
    Assignee: Bristol-Myers Company
    Inventors: James A. Matson, James A. Bush
  • Phenylalanine ammonia lyase-producing microbial cells
    Patent number: 4598047
    Abstract: A method for producing and isolating catabolite resistant, phenylalanine ammonia-lyase-producing microorganisms is disclosed. Also disclosed are novel catabolite resistant organisms and a method for producing L-phenylalanine using such organisms.
    Type: Grant
    Filed: June 25, 1984
    Date of Patent: July 1, 1986
    Assignee: Genex Corporation
    Inventor: Jeffrey C. McGuire
  • Preparation of a maltogenic amylase enzyme
    Patent number: 4598048
    Abstract: A maltogenic amylase enzyme with improved thermostability, which can be produced by cultivating Bacillus strain NCIB 11837 belonging to the Bacillus stearothermophilus complex, is made by cultivation of a host microorganism transformed with the gene coding for the maltogenic amylase enzyme.
    Type: Grant
    Filed: March 20, 1984
    Date of Patent: July 1, 1986
    Assignee: Novo Industri A/S
    Inventors: Boerge K. Diderichsen, Lars Christiansen
  • Method for facilitating externalization of proteins synthesized in bacteria
    Patent number: 4595658
    Abstract: A method of externalizing proteins from the periplasmic space of gram-negative bacteria and in particular, E. coli and its relatives, comprising utilizing bacteria which have a phage gene, coding for a protein (such as gene III protein) or a mutant bacterial gene (such as a gene coding for a membrane function) which causes perturbation of the outer bacterial membrane resulting in leakage of proteins in the periplasmic space from the cell.
    Type: Grant
    Filed: September 13, 1982
    Date of Patent: June 17, 1986
    Assignee: The Rockefeller University
    Inventors: Norman D. Zinder, Peter Model, Jef D. Boeke
  • Glucose or maltose from starch
    Patent number: 4594322
    Abstract: A process for obtaining glucose from thinned starch by partially hydrolyzing the latter to give from 50% to 92% glucose followed by separation of the hydrolysis product to afford a glucose-enriched product with recycling of the glucose-depleted stream affords benefits unattainable by conventional commercial processes. Substantial reductions in process time and reversion products and a substantial increase in productivity are among some of the benefits.
    Type: Grant
    Filed: May 10, 1984
    Date of Patent: June 10, 1986
    Assignee: UOP Inc.
    Inventors: Gregory J. Thompson, Kaung-Far Lin, David W. Penner
  • High temperature isomerization process
    Patent number: 4593001
    Abstract: The invention relates to a method for producing a glucose isomerizing enzyme which is functional at temperatures over 90.degree., utilizing a strain microorganisms having the identifying characteristics of Arthrobacter sp. ATCC 21748.
    Type: Grant
    Filed: January 9, 1984
    Date of Patent: June 3, 1986
    Assignee: Nabisco Brands, Inc.
    Inventor: Robert O. Horwath
  • Strains of Aspergillus and use thereof to produce amylolytic enzymes
    Patent number: 4593005
    Abstract: A novel strain Aspergillus K27 belonging to genus Aspergillus which has the same taxonomical characteristics as those of Aspergillus fumigatus except that(1) its conidiophore is colorless and(2) its growing temperature range is between 10.degree. and 55.degree. C., which produces amylolytic enzymes which can hydrolyze alpha-amylase resistant starch as well as usual starch.
    Type: Grant
    Filed: August 6, 1984
    Date of Patent: June 3, 1986
    Assignee: Daikin Industries, Ltd.
    Inventor: Susumu Hizukuri
  • Process for the purification of dextran-sucrase
    Patent number: 4591563
    Abstract: The invention is directed to a process for the purification of the dextran-sucrase produced by Leuconostoc mesenteroides bacteria.The process according to the invention consists of adding to the culture medium, that contains the extra-cellular enzyme and dextran, a quantity of a PEG type polyether so that, in the medium appear two non miscible phases, that are thus maintained under stirring in order to obtain a good contact. Thereafter the lower dextran phase is separated from the upper polyether phase in order to provide a dextran-sucrase enriched enzymatic preparation.The purified enzyme can be used in the synthesis processes of the dextrans possessing specific molecular weights for certain applications.
    Type: Grant
    Filed: May 3, 1984
    Date of Patent: May 27, 1986
    Assignee: Societe Nationale Elf Aquitaine
    Inventors: Francois Paul, Pierre Monsan, Daniel Auriol
  • Transferase enzymes which modify the 3'-termini of ribonucleic acid and methods
    Patent number: 4591564
    Abstract: Three ribonucleotidyl terminal transferase enzymes are disclosed which modify the 3'-termini of ribonucleic acid (RNA) molecules by the addition of ribonucleotide units using ribonucleoside triphosphates as substrates. These terminal transferase activities are distinguishable by the specific ribonucleotide (e.g. AMP, CMP, or UMP) transferred to the 3'-hydroxyl terminus of an RNA primer. Also provided is a method for the 3'-terminal modification of RNA molecules by these enzymes and sequencing of RNA from its 3'-termini. The methods provide a convenient and efficient procedure for 3'-terminal modification (homopolymer tailing) of RNA required for synthesis of complete complementary DNA (cDNA) copies or double-stranded DNA gene copies by retrovirus-associated reverse transcriptase. Using the enzymes of the invention, RNA can also be radiolabelled to very high levels for molecular hybridization.
    Type: Grant
    Filed: November 10, 1982
    Date of Patent: May 27, 1986
    Assignee: The University of Montana
    Inventor: Kenneth F. Watson
  • Microbiologically produced L-phenylalanine-dehydrogenase, process for its recovery and use
    Patent number: 4590161
    Abstract: The invention is directed to a microbiologically produced L-phenylalanine-dehydrogenase and a process for its recovery from Brevibacterium species DSM 2448. The new enzyme can be used for the enzymatic conversion of phenyl pyruvic acid, p-hydroxyphenyl pyruvic aid, indolyl pyruvic acid or 2-keto-4-(methylmercapto)-butyric acid into the corresponding L-.alpha.-aminocarboxylic acids.
    Type: Grant
    Filed: February 24, 1984
    Date of Patent: May 20, 1986
    Assignees: Degussa Aktiengesellschaft, Gesellschaft fur biotechnologisch Forschung
    Inventors: Maria-Regina Kula, Werner Hummel, Horst Schutte, Wolfgang Leuchtenberger
  • Preparation of the enzyme .beta.-glucanase by fermentation of fungi
    Patent number: 4588690
    Abstract: Process for preparation of .beta.-glucanase by cultivation of species of the fungus Rhizomucor pusillus (Lindt) Schipper under aerobic and thermophilic conditions. The enzyme .beta.-glucanase is an important component for degradation of polysaccharides into shorter molecular units.
    Type: Grant
    Filed: August 15, 1984
    Date of Patent: May 13, 1986
    Assignee: Norsk Hydro a.s.
    Inventors: Bernt A. Nissen, Jon Hovland
  • Process for the production of muconic acid
    Patent number: 4588688
    Abstract: This invention provides a process for bioconversion of an organic substrate (e.g., ethylbenzene or catechol) to muconic acid.This invention further provides a procedure for constructing novel strains of microorganisms (e.g., Pseudomonas putida Biotype A) which are capable of converting an organic substrate to muconic acid quantitatively by the ortho (catechol 1,2-oxygenase) pathway.Muconate lactonizing enzyme is not induced in the microorganisms, thereby permitting the muconic acid to be produced and accumulated in a quantity greater than one gram of muconic acid per liter of growth medium.
    Type: Grant
    Filed: July 22, 1983
    Date of Patent: May 13, 1986
    Assignee: Celanese Corporation
    Inventor: Peter C. Maxwell
  • Highly thermostable glucoamylaseand process for its production
    Patent number: RE32153
    Abstract: Process for the production of a thermostable glucoamylase by a strain of Talaromyces and the glucoamylase produced thereby.
    Type: Grant
    Filed: August 2, 1985
    Date of Patent: May 20, 1986
    Assignee: CPC International Inc.
    Inventors: Masaki Tamura, Mizuho Shimizu, Minoru Tago