Patents Examined by Lionel M. Shapiro
  • Antibiotics, neoviridogriseins, and their method of production
    Patent number: 4536397
    Abstract: A group of novel depsipeptide antibiotics, neoviridogriseins I, II and III are produced by fermentation of Streptomyces sp. P8648 (FERM-P 3562; ATCC 31289). The antibiotics are highly active against Gram-positive bacteria and mycoplasmas.
    Type: Grant
    Filed: October 31, 1983
    Date of Patent: August 20, 1985
    Assignees: Sanraku-Ocean Co., Ltd., Panlabs, Inc.
    Inventors: Yasushi Okumura, Kazuhiko Okamura, Yasuo Fugakawa, Tomoyuki Ishikura, Kageaki Kouno, Joseph Lein
  • Thermostable glucoamylase and method for its production
    Patent number: 4536477
    Abstract: This invention relates to a glucoamylase enzyme exhibiting thermostability at pH values between 6 and 7 which is derived from a spore-forming, thermophilic, anaerobic bacterium and to a process for its production. The glucoamylase is especially useful for the preparation of glucose-containing syrups from starch.
    Type: Grant
    Filed: August 17, 1983
    Date of Patent: August 20, 1985
    Assignee: CPC International Inc.
    Inventors: Dennis M. Katkocin, Nancy S. Word, Shiow-Shong Yang
  • Recovery of acid fungal protease
    Patent number: 4532213
    Abstract: Glucoamylase and acid fungal protease co-produced by the fermentation of fungal species capable of producing these enzymes are separated by carrying out the fermentation at a pH at which the protease associates itself with the fungal mycelium and removing the mycelium from the fermentation broth. The mycelium is then resuspended in an aqueous medium and the pH adjusted to cause the dissociation of the protease and mycelium with subsequent recovery of the protease from the aqueous medium.
    Type: Grant
    Filed: December 8, 1983
    Date of Patent: July 30, 1985
    Assignee: Miles Laboratories, Inc.
    Inventors: Jayarama K. Shetty, James J. Marshall
  • Constitutive glucose isomerase producer
    Patent number: 4532208
    Abstract: A mutant Streptomyces coelicolor, NRRL 15398, produces glucose isomerase constitutively at a level at least as great as the parent does inductively in common growth media. The isomerase can be effectively used to isomerize glucose to fructose in a continuous process using immobilized enzyme.
    Type: Grant
    Filed: May 5, 1983
    Date of Patent: July 30, 1985
    Assignee: UOP Inc.
    Inventors: Edmund W. Hafner, Denise M. Jackson
  • L-2-hydroxy-4-methylpentanoic acid-dehydrogenase, process for obtaining it and its use
    Patent number: 4530903
    Abstract: The purpose of the invention is the new enzyme L-2-hydroxy-4-methylpentanoic acid-dehydrogenase and its recovery from Lactobacillus confuses. The new enzyme can be used to enzymatically change L-2-hydroxy-4-methylpentanoic acid and various other L-2-hydroxy-carboxylic acids into the corresponding 2-ketocarboxylic acids or 2-keto-4-methylpentanoic acid and various other 2-ketocarboxylic acids into the corresponding L-2-hydroxycarboxylic acids.
    Type: Grant
    Filed: September 13, 1983
    Date of Patent: July 23, 1985
    Assignee: Degussa Aktiengelleschaft
    Inventors: Wolfgang Leuchtenberger, Maria-Regina Kula, Werner Hummel, Horst Schutte
  • Microbial rennet having increased milk coagulating activity and method and method for production thereof
    Patent number: 4530906
    Abstract: A method for increasing the milk coagulating activity of microbial rennet obtained from Mucor pusillus microorganisms, which comprises acylating the microbial rennet in an aqueous medium by contacting the microbial rennet with succinic anhydride at a temperature of about 0.degree. C. to about 40.degree. C. and a pH of about 4 to about 10 for a time sufficient to obtain the desired increase in milk coagulating activity; and improved microbial rennet having a low PA/MCA index and a high MCA index and obtained by the aforesaid method.
    Type: Grant
    Filed: April 5, 1983
    Date of Patent: July 23, 1985
    Assignee: Meito Sangyo Kabushiki Kaisha
    Inventors: Toshihiko Higashi, Yoshinori Kobayashi, Shinjiro Iwasaki
  • Process for producing L-glutamic acid by fermentation
    Patent number: 4529697
    Abstract: A process for producing L-glutamic acid by fermentation is disclosed, which process comprises culturing aerobically in a culture medium a mutant of the genus Brevibacterium or Corynebacterium which has an increased superoxide dismutase activity and is capable of producing L-glutamic acid in the culture medium and recovering the L-glutamic acid. The yield of L-glutamic acid can be increased by using the aforementioned mutants.
    Type: Grant
    Filed: March 15, 1983
    Date of Patent: July 16, 1985
    Assignee: Ajinomoto Co., Inc.
    Inventors: Minoru Yoshimura, Yosuke Koyama, Koichi Goto, Sumio Inoue, Shigeho Ikeda, Hiroe Yoshii
  • Process for liquefaction of starch
    Patent number: 4529696
    Abstract: A liquid starch solution suitable for saccharification into a maltose-containing product is obtained by suspending starch in water, adding to the starch suspension a heat resistant .alpha.-amylase and a carbonate, adjusting the resultant mixture to a pH value in the range of from 7.5 to 8.0, and thermally treating the resultant starch milk while repressing possible hydrolysis of starch molecules.
    Type: Grant
    Filed: June 30, 1983
    Date of Patent: July 16, 1985
    Assignees: Agency of Industrial Science and Technology, Ministry of International Trade and Industry
    Inventors: Yoshiyuki Takasaki, Yoshimasa Takahara, Chikashi Izumi, Atsushi Mori, Masahiko Nishiguchi, Masaru Yamada
  • Processes and products involving cell modification
    Patent number: 4528265
    Abstract: Living mammalian cells are modified by subjecting them to the influence of the ions from a metal electrode, for example of silver, which is placed in contact with them and which is made electrically positive, and causing a low intensity direct current to flow through them. The cells, which are relatively specialized, such as normal mammalian fibroblasts, assume a simpler, relatively unspecialized form and come to resemble hematopoetic or marrow-like cells.
    Type: Grant
    Filed: May 11, 1982
    Date of Patent: July 9, 1985
    Inventor: Robert O. Becker
  • Angiotensin I-converting enzyme inhibitor and preparation of the same
    Patent number: 4526866
    Abstract: A novel enzyme inhibitor of angiotensin I-converting enzyme was produced from an actinomycetes belonging to genus Streptomyces. This enzyme inhibitor is a peptide, and specifically inhibits the angiotensin I-converting enzyme, but does not inhibit thermolycin, pepsin and .alpha.-chymotrypsin.
    Type: Grant
    Filed: April 5, 1983
    Date of Patent: July 2, 1985
    Assignee: Fujizoki Pharmaceutical Co., Ltd.
    Inventors: Yasuji Kido, Tsutomu Yoshida, Toshinari Hamakado, Masami Anno, Takahiro Harada, Yoshinobu Motoki
  • Method for the production of sphingomyelinase
    Patent number: 4525458
    Abstract: The invention is to provide a method for the production of sphingomyelinase, by culturing in a medium a sphingomyelinase-producing strain belonging to genus Streptomyces and collecting the resulting sphingomyelinase from the cultured medium. The strain, Streptomyces sp. A 9107, newly separated from a soil, is found preferable for the method of the present invention, and has been deposited as NRRL 15100 and FERM-P 4978.
    Type: Grant
    Filed: September 1, 1982
    Date of Patent: June 25, 1985
    Assignee: Toyo Jozo Company, Ltd.
    Inventors: Shigeyuki Imamura, Hideo Misaki, Naoki Muto
  • Production of L-4-phenyl-2-aminobutanoic acid by transamination
    Patent number: 4525454
    Abstract: A process is described for producing L-4-phenyl-2-amino butanoic acid. The process comprises reacting 4-phenyl-2-oxobutanoic acid or an ester thereof with L-aspartic acid in the presence of transaminase enzyme to produce L-4-phenyl-2-aminobutanoic acid and oxaloacetate, and decarboxylating said oxaloacetate.
    Type: Grant
    Filed: August 23, 1984
    Date of Patent: June 25, 1985
    Assignee: Genetics Institute, Inc.
    Inventor: J. David Rozzell
  • Hybridoma cell lines and monoclonal antibodies to theophylline
    Patent number: 4524025
    Abstract: Monoclonal antibodies to theophylline having 5% or less cross-reactivity with caffeine and the continuous hybrid monoclonal cell lines for their production are provided. These antibodies are useful in a particle-enhanced turbidimetric inhibition immunoassay for theophylline.
    Type: Grant
    Filed: August 9, 1982
    Date of Patent: June 18, 1985
    Assignee: E. I. Du Pont de Nemours and Company
    Inventor: John E. Geltosky
  • Bacterial strain for purifying hydrocarbons pollution and purification process
    Patent number: 4521515
    Abstract: A bacterial strain comprising a strain of Pseudomonas exhibits acceleration of growth in a logarithmic phase in a trophic culture medium when hydrocarbons are added thereto, a process for producing such a bacterial strain, and a purification process using such a bacterial strain.
    Type: Grant
    Filed: October 21, 1981
    Date of Patent: June 4, 1985
    Assignee: Seiken Kai Foundation Juridical Person
    Inventor: Kosei Hata
  • Process for producing an addition compound of a dipeptide ester and an amino acid ester
    Patent number: 4521514
    Abstract: A process for producing an addition compound of N-benzyloxycarbonyl-.alpha.-L-aspartyl-L-phenylalanine methyl ester and phenylalanine methyl ester. Benzyloxycarbonylation of the amino group of L-aspartic acid by benzyloxycarbonylchloride in the presence of a base and esterification of phenylalanine containing its L-body by methanol in the presence of an acid are carried out and both the reaction solutions are admixed after residual methanol is removed from the esterification reaction solution. A proteolytic enzyme is added to the resulting mixture solution after it is adjusted to have a pH, value, at which no substantial deactivation of the enzyme occurs. The reaction of N-benzyloxycarbonyl-L-aspartic acid and phenylalanine methyl ester is carried out in the solution at a pH, at which the proteolytic enzyme exerts the enzymatic activity to deposit the addition compound which is then recovered.
    Type: Grant
    Filed: July 22, 1983
    Date of Patent: June 4, 1985
    Assignees: Toyo Soda Manufacturing Co., Ltd., Ajinomoto Co., Inc., Sagami Chemical Research Center
    Inventors: Kiyotaka Oyama, Shigeaki Irino, Tsuneo Harada, Masao Nakamura
  • Hybridoma cell lines and monoclonal antibodies to theophylline
    Patent number: 4521510
    Abstract: Monoclonal antibodies to theophylline having 5% or less cross-reactivity with caffeine and the continuous hybrid monoclonal cell lines for their production are provided. These antibodies are useful in a particle-enhanced turbidimetric inhibition immunoassay for theophylline.
    Type: Grant
    Filed: January 3, 1984
    Date of Patent: June 4, 1985
    Assignee: E. I. Du Pont de Nemours and Company
    Inventor: John E. Geltosky
  • Tissue culture and cell growth-promoting material and its method of manufacture
    Patent number: 4520107
    Abstract: This invention relates to a novel cell growth-promoting material for use as a supplement to basal tissue culture media for the cultivation of animal cells in vitro and to the method for its preparation. More particularly, it relates to an infusion consisting of growth-promoting material from adult, calf or fetal bovine blood clots as a supplement to basal media for cultivation of animal cells in vitro.
    Type: Grant
    Filed: September 28, 1982
    Date of Patent: May 28, 1985
    Assignee: Polydex Chemicals Ltd.
    Inventors: George M. Healy, Kenneth D. Curry
  • Production of L-amino acids by transamination
    Patent number: 4518692
    Abstract: A process is described for producing alpha amino acids or derivatives thereof. The process comprises reacting an alpha-keto acid with L-aspartic acid in the presence of transaminase enzyme to produce (1) an alpha amino acid corresponding to said alpha-keto acid and (2) oxaloacetate; and decarboxylating said oxaloacetate.
    Type: Grant
    Filed: September 1, 1983
    Date of Patent: May 21, 1985
    Assignee: Genetics Institute, Inc.
    Inventor: J. David Rozzell
  • Acid stable protease from mutants of genus Aspergillus
    Patent number: 4518697
    Abstract: Process for the preparation of acid stable protease having a broad activity spectrum in high yields from mutants of the fungus Aspergillus, and the mutants used therein.
    Type: Grant
    Filed: December 13, 1982
    Date of Patent: May 21, 1985
    Assignee: Henkel Kommanditgesellschaft auf Aktien
    Inventors: Friedhelm Bartnik, Joachim Schindler, Albrecht Weiss, Rolf Schmid
  • Process for eluting egg white lysozyme
    Patent number: 4518695
    Abstract: In the process of eluting egg white lysozyme by contacting a weakly acidic cation exchange resin having egg white lysozyme adsorbed thereon with an eluting agent comprising a salt solution, an alkali agent is added to the eluate containing the weakly acidic cation exchange resin having egg white lysozyme adsorbed thereon whereby the egg white lysozyme can be eluted more effectively. From the eluate thus obtained, lysozyme can be collected in a high yield by an ordinary collecting method such as salting out.
    Type: Grant
    Filed: November 23, 1982
    Date of Patent: May 21, 1985
    Assignee: Kewpie Kabushiki Kaisha
    Inventor: Mineo Hasegawa