Patents Examined by Lyle A. Alexander
  • Patent number: 10502720
    Abstract: Methods are provided for making rapid labeled dextran ladders and other calibrants useful in liquid chromatography. The methodologies include a two-step process comprising a reductive amination step of providing a reducing glycan and reacting it with a compound having a primary amine to produce an intermediate compound. The intermediate compound is then rapidly tagged with a rapid tagging reagent to produce the rapid labeled dextran ladder.
    Type: Grant
    Filed: November 12, 2015
    Date of Patent: December 10, 2019
    Assignee: Waters Technologies Corporation
    Inventors: Michael F. Morris, Matthew A. Lauber, Darryl W. Brousmiche
  • Patent number: 10493448
    Abstract: In one arrangement, a cartridge includes a cartridge body defining a holding compartment, first and second fractioning compartments, and a number of flow channels formed within the cartridge body. A predetermined quantity of fluid can be held in the holding compartment when the cartridge body is held in a first orientation, and can be poured from the holding compartment to the first fractioning compartment by rotating the cartridge body about a predefined rotation axis to a second orientation, spilling the fluid from the holding compartment to the first fractioning compartment through one of the flow channels. The first fractioning compartment is such that when the cartridge body is in the second orientation, not all of the fluid can be contained in the first fractioning compartment, and fluid that overflows the first fractioning compartment flows through a second flow channel to the second fractioning compartment.
    Type: Grant
    Filed: March 8, 2016
    Date of Patent: December 3, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Glenn Price, Chris Charlton, William Link, Robert Alan Iovanni, Anthony F. Prestigiacomo
  • Patent number: 10449502
    Abstract: The present invention is generally related to the analysis of chemical compositions of hydrocarbons and hydrocarbon blends. This method, in particular embodiments, may apply specifically to the problem of analyzing extremely complex hydrocarbon-containing mixtures when the number and diversity of molecules makes it extremely difficult or impossible to realistically identify and quantify them individually in a reasonable timeframe. Particular SEC (size exclusion chromatography)-based methods and apparatus disclosed herein may be used to measure, e.g., the molecular size, weight, and/or volume, whether in absolute or relative manner, of the various components of eluate from the SEC stationary phase (e.g., a permeable gel). This analytical method is applicable on a wide variety of hydrocarbonaceous materials, and especially useful for, but not limited to oil, maltenes of oil, asphalt binders and asphalt binder blends, which may contain wide varieties of different types of additives, modifiers, and chemistries.
    Type: Grant
    Filed: November 7, 2018
    Date of Patent: October 22, 2019
    Assignee: The University of Wyoming Research Corporateion
    Inventors: Joseph F. Rovani, Jr., Jean-Pascal Planche, Ryan Bradley Boysen
  • Patent number: 10451598
    Abstract: A device for detecting an airborne contaminant includes (a) a reactive polymer film having affinity for binding with the airborne contaminant, (b) an electrically conductive polymer film in contact with the reactive polymer film and having electrical property sensitive to binding of the airborne contaminant to the reactive polymer film, and (c) two electrodes in electrical contact with the electrically conductive polymer film for measuring the electrical property to detect the binding of the airborne contaminant to the reactive polymer film. Another device for detecting an airborne contaminant includes (a) a polymer film molecularly imprinted with the airborne contaminant, and (b) color reporting molecules having color sensitive to binding of the airborne contaminant to the polymer film. A method for manufacturing a device for detecting an airborne contaminant includes depositing, using one or more inkjet print heads, a polymer film and at least two electrodes onto a substrate.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: October 22, 2019
    Assignee: THE TRUSTEES OF DARTMOUTH COLLEGE
    Inventors: Joseph J. Belbruno, Susanne E. Tanski
  • Patent number: 10434513
    Abstract: A method and microfluidic device are provided for containing a droplet having an outer surface at a predetermined location. The microfluidic device includes a plate having an upper surface and a central region communicating with the upper surface. The central region is adapted for receiving a droplet of fluid thereon. The central region includes an outer periphery that defines a first fluid constraint configured for discouraging fluid on the central region from flowing therepast. A second fluid constraint extends about the first fluid constraint. The second fluid constraint is configured for discouraging fluid flowing therepast. A third fluid constraint extends about the second fluid constraint. The third fluid constraint configured for discouraging fluid flowing therepast.
    Type: Grant
    Filed: June 8, 2016
    Date of Patent: October 8, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: David J. Beebe, David J. Guckenberger, Jr., Scott M. Berry, Hannah M. Pezzi
  • Patent number: 10434508
    Abstract: A capsule (100) comprising a housing (110) in which are disposed nanofluidic biosensors (120), a fluid connecting element (140), a filter (150) and a cover (160) is described. The capsule (100) allows the analysis of a fluid sample (300) that would be deposited in the capsule system (100) by a pipette (400). The fluid sample (300) is filtered when passing through a filter (150), then transferred by a fluid connecting element (140) to the inlets of one or several nanofluidic biosensors (120). The capsule system (100) is disposed on an external support (200), and finally an optical or an electrical measurement unit (500) is used to measure the molecular interactions in the nanofluidic biosensors.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: October 8, 2019
    Assignee: Abionic SA
    Inventors: Iwan Maerki, Lauriane Carrere, Nicolas Durand
  • Patent number: 10436790
    Abstract: Mass spectrometry (MS) active, fluorescent rapid tagging reagent is provided having three substituent groups: (a) a tertiary amino group or other MS active atom; (b) a highly fluorescent moiety; and (c) a reactive group that can react with an amine. The reactive group provides rapid tagging of desired bio-molecules. The fluorescent moiety provides the fluorescent signal. The tertiary amino group provides the MS signal.
    Type: Grant
    Filed: August 13, 2014
    Date of Patent: October 8, 2019
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Darryl W. Brousmiche, Ying-Qing Yu, Matthew Lauber
  • Patent number: 10416166
    Abstract: Reagents comprising MS active, fluorescent molecules with an activate functionality for reaction with amines useful in rapid tagging of biomolecules such as N-glycans, proteins, peptides, and amino acids, and uses thereof are taught and described. These MS active, fluorescent molecules may have three functional components such as a tertiary amino group or other MS active atom, a highly fluorescent moiety, and a functional group that rapidly reacts with amines.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: September 17, 2019
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Darryl W. Brousmiche, Ying-Qing Yu
  • Patent number: 10408815
    Abstract: One embodiment of the present invention provides an apparatus for analyzing substances included in a liquid. The apparatus includes an absorber for absorbing the liquid and a testing device separate from the absorber. The absorber includes a main body and a detachable strip embedded in the main body, and both the main body and the detachable strip absorb the liquid. The testing device includes one or more sensors positioned on a top surface of the testing device to provide information associated with the substances included in the liquid in response to the detachable strip being placed on the top surface of the testing device.
    Type: Grant
    Filed: December 30, 2016
    Date of Patent: September 10, 2019
    Assignee: PALO ALTO RESEARCH CENTER INCORPORATED
    Inventors: Michael Kuniavsky, Siqi Wang
  • Patent number: 10401347
    Abstract: Disclosed are methods, materials and devices for approximation of blood volume in a fluid, such as in a biological fluid collected during a surgical procedure. The method and devices include the use of a RBC flocculant, such as polyDADMAC, and an approximate blood hematocrit for the type of animal, as well as a calculated RBC packing ratio corresponding to the collection device being used. Also provided is a Blood Indicator Panel (BIP), comprising a series of markings calculated from an observed red blood settlement volume, the average animal type hematocrit, and a calculated RBC packing ratio “?” value for the collection device. Pediatric (about 200 ml or 250 ml size container), adult human (about 1,000 ml-1,500 ml) and veterinary (about 500 ml-2,500 ml) collection containers are also disclosed, that include a RBC flocculant, for use in approximating blood volume in a fluid.
    Type: Grant
    Filed: January 11, 2018
    Date of Patent: September 3, 2019
    Assignee: Cypher Medical, LLC
    Inventors: Christopher A. Carew, Jian Ling
  • Patent number: 10393749
    Abstract: The present invention addresses the problem of identifying a biomarker of renal failure, said biomarker being available from urine or blood, and fluctuating from an early stage than glomerular filtration rate and serum creatinine level, and thus developing a technique for diagnosing early stage kidney failure. A method for analyzing the blood, plasma, serum or urine of a renal failure suspected subject comprises a step of measuring the concentration of a pair of D-form and L-form of at least one amino acid selected from the amino acid group consisting of [D-serine] and [L-serine], etc., contained in the blood, plasma, serum or urine of the subject, and calculating, as an pathological index of the subject, the ratio of the D-form concentration to the L-form concentration or the percentage of the D-form concentration relative to the total concentration of the D-form and L-form.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: August 27, 2019
    Assignee: Shiseido Company, Ltd.
    Inventors: Kenji Hamase, Yurika Miyoshi, Masashi Mita
  • Patent number: 10393666
    Abstract: A method of monitoring a respiratory stream can be provided by monitoring color change of a color change material to determine a CO2 level of the respiratory stream in contact with the color change material by emitting visible light onto the color change material. Related devices, systems, and compositions are also disclosed.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: August 27, 2019
    Assignee: RESPIRION, LLC
    Inventors: Eugene W. Moretti, Robert Lavin Wood, Allan Bruce Shang, Steven S. Yauch
  • Patent number: 10391488
    Abstract: A microfluidic probe head for providing a sequence of separate liquid volumes separated by spacers, the separate liquid volumes including a respective target substance associated with a respective target area, the microfluidic probe head including an inlet and an outlet; a first fluid channel fluidly connected to the inlet, the first fluid channel configured for delivering an injection liquid from the inlet to a respective target area; a second fluid channel fluidly connected to the outlet, the second fluid channel configured for delivering liquid volumes from the respective target area to the outlet; and a spacer insertion unit fluidly connected to the second fluid channel, the spacer insertion unit configured for inserting spacers into the second fluid channel between the liquid volumes to provide the sequence of separate liquid volumes separated by spacers.
    Type: Grant
    Filed: February 11, 2016
    Date of Patent: August 27, 2019
    Assignee: International Business Machines Corporation
    Inventors: Julien Autebert, Emmanuel Delamarche, Govind Kaigala, Aditya Kashyap, Xander Frank Van Kooten
  • Patent number: 10384209
    Abstract: A microfluidic platform including a microfluidic layer and a contact layer. The microfluidic layer is embedded with a microfluidic structure including a micro-channel and a fluidic sample contained in the micro-channel. The contact layer is able to be attached to the microfluidic layer, and includes a first heater for heating a first area of the microfluidic structure to a first temperature and a second heater for heating a second area of the microfluidic structure to a second temperature. The microfluidic layer and the contact layer rotate together during operation. A method for controlling a sample in the micro-channel of the microfluidic structure.
    Type: Grant
    Filed: September 10, 2012
    Date of Patent: August 20, 2019
    Assignees: THE CHINESE UNIVERSITY OF HONG KONG, NANYANG TECHNOLOGICAL UNIVERSITY, HOSPITAL AUTHORITY
    Inventors: Qiu Lan Chen, Ho Pui Ho, Siu Kai Kong, Patrick Kwok Leung Kwan, Yiu Wa Kwan, Ho Chin Kwok, Ping Shum, Yick Keung Suen, Shu Yuen Wu, Alice Kar Lai Yang, Jun Qiang Zhou
  • Patent number: 10379104
    Abstract: An exposure apparatus for carrying out in vitro experiments with biological test systems, which are perfused with an exposure medium, forming an exposure atmosphere, includes an exposure cap, which is dimensioned in such a manner that it can be set onto a standard multiwell plate and connected with the standard multiwell plate using suitable connecting elements.
    Type: Grant
    Filed: April 29, 2014
    Date of Patent: August 13, 2019
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forshcung e.V.
    Inventors: Detlef Ritter, Jan Knebel
  • Patent number: 10376884
    Abstract: Systems and methods disclosed herein related to an apparatus including a fluid flow plate and a microfluidic valve assembly. The fluid flow plate includes a plurality of polymer layers that define a fluid flow passage through the microfluidic valve assembly. The microfluidic valve assembly includes a valve seat, a flexible membrane, a valve cavity, a valve head, and an actuator. The actuator is configured to selectively control pressure applied by the valve head to the flexible membrane, such that in a first actuator state the valve head depresses the flexible membrane into the valve cavity and into contact with the valve seat, thereby preventing fluid flow through the valve assembly, and in a second state, the valve head and the flexible membrane are retracted substantially out of the valve cavity allowing fluid to flow through the valve assembly. In various implementations, the valve seat and/or the flexible membrane include an elastomer layer.
    Type: Grant
    Filed: February 4, 2016
    Date of Patent: August 13, 2019
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Mark Joseph Mescher, Jonathan Robert Coppeta, Abigail June Spencer, Brett Isenberg
  • Patent number: 10369572
    Abstract: A reagent dispensing apparatus includes a housing having a cavity with an opening and an aperture; and a plunger that includes a first member, that fits through the aperture, that is associated with a first seal. The plunger includes a second member associated with the first seal and a second seal that together form a first chamber, within the cavity, in which a first reagent is stored. The plunger includes a safety mechanism that controls movement of the plunger within the cavity. The apparatus includes a frangible seal that covers the opening and together with the second seal forms a second chamber, within the cavity, that stores a second reagent. When the first end is depressed and the safety mechanism is disengaged, the frangible seal is breached causing the evacuation of the first reagent or the second reagent through the opening.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: August 6, 2019
    Assignee: Point of Care Diagnostics, LLC
    Inventor: William Francis Marion, Jr.
  • Patent number: 10359441
    Abstract: The invention relates to a reagent station (1) for an automated analysis device, comprising a first and a second reagent container storage (2, 10) and a transfer apparatus (20) which transfers reagent containers (16) between the first and the second reagent container storage (2, 10). Furthermore, the invention relates to a method for loading an automated analysis device with reagent containers.
    Type: Grant
    Filed: November 11, 2013
    Date of Patent: July 23, 2019
    Assignee: Siemens Healthcare Diagnostics Products GmbH
    Inventor: Holger Pufahl
  • Patent number: 10330574
    Abstract: A biological fluids concentration device, including a tube-in-tube assembly, is disclosed. The tube-in-tube assembly receives biologic fluids and may then be placed in the bucket of a centrifuge and spun to separate out the components of the biological fluid by their various densities. For example, whole blood may be centrifuged in the tube-in-tube assembly for separating into plasma, red blood cell component, and a buffy coat. A piston slideably and sealingly engages an inner tube of the tube-in-tube assembly, the inner tube fitting within an outer tube. A lid is designed to engage the top of the outer tube, which lid has an opening therein for receipt of a plunger. The plunger is adapted to move up and down with respect to the lid and the tubes, so as to sealingly, in a down position, and unsealingly, in an open position, engage the top of the inner tube of the tube-in-tube assembly.
    Type: Grant
    Filed: October 30, 2017
    Date of Patent: June 25, 2019
    Assignee: ALLIANCE PARTNERS, LLC
    Inventors: Emad Hassouneh, Logan White, Matt Schmidt, Andrew Enke, Frank Morris
  • Patent number: 10295447
    Abstract: An energized dispersive extraction method for sample preparation for analysis is disclosed. The method includes the steps of placing an extraction solvent, sorbent particles, and a sample matrix containing an analyte in a heat conductive sample cup; positioning the sample cup in a pressure-resistant reaction chamber; dispersing the solvent and the sample matrix in the sample cup in the reaction chamber; heating the sample matrix and the solvent in the sample cup in the reaction chamber to a temperature that generates an above-atmospheric pressure; draining the solvent extract from the sample cup at atmospheric pressure; and collecting the solvent extract for analysis.
    Type: Grant
    Filed: July 10, 2017
    Date of Patent: May 21, 2019
    Assignee: CEM Corporation
    Inventors: Michael J. Collins, Sr., Joseph J. Lambert, Matthew N. Beard, Paul C. Elliott