Abstract: The present invention provides an exchangeable, reagent pre-loaded sheets which can be temporarily applied to an electrode array on a digital microfluidic device (DMF). The substrate facilitates virtually un-limited re-use of the DMF devices avoiding cross-contamination on the electrode array itself, as well as enabling rapid exchange of pre-loaded reagents while bridging the world-to-chip interface of DMF devices. The present invention allows for the transformation of DMF into a versatile platform for lab-on-a-chip applications.
Type:
Grant
Filed:
October 1, 2008
Date of Patent:
May 29, 2012
Assignee:
The Governing Council of the University of Toronto
Inventors:
Aaron R. Wheeler, Irena Barbulovic-Nad, Hao Yang, Mohamed Abdelgawad
Abstract: The present invention provides a system and method for detecting antigens captured on an antibody array. The method comprises the following steps of providing the antibody array having at least two antibodies, contacting the antibody array with a sample containing at least one antigen that may be captured by the antibodies disposed on the antibody array, and detecting the at least one antigen captured by the antibody array with a detecting agent that specifically binds to the antigen-bound antibodies on the antibody array, thereby the at least one antigen captured by the antibody array can be detected independent of the structures of the antigens. In a preferred embodiment, C1q is used as the detecting agent to detect antigen-bound antibodies.
Abstract: An electrochemical immunosensor system with reduced interference, comprising: a first immunosensor that generates an electrochemical signal based on the formation of a sandwich between an immobilized antibody, a target analyte and a labeled antibody, wherein a portion of the signal arises from non-specific binding of the labeled antibody in the region of the first immunosensor, and a second immunosensor that acts as an immuno-reference sensor and generates a signal that is the same as or predictably related to the degree of non-specific binding which occurs in the region of the first immunosensor, and has an immunocomplex between an immobilized antibody and an endogenous or exogenous protein that is in the sample and that is not the target analyte.
Type:
Grant
Filed:
February 18, 2010
Date of Patent:
May 1, 2012
Assignee:
Abbott Point of Care Inc.
Inventors:
Cary James Miller, John Lewis Emerson Campbell
Abstract: The invention provides devices, test kits and methods for removing target agents from a sample. The device contains one or more porous matrices having pore sizes larger than 10 ?m, and a plurality of particles impregnated therein. The target agents attach the device and are removed from the sample.
Type:
Grant
Filed:
June 9, 2005
Date of Patent:
April 24, 2012
Assignee:
Pathogen Removal and Diagnostic Technologies, Inc.
Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
Type:
Grant
Filed:
January 9, 2007
Date of Patent:
April 24, 2012
Assignee:
Applied Biosystems, LLC
Inventors:
Timothy Woudenberg, Michael Albin, Reid B. Kowallis, Yefim Raysberg, Robert P. Ragusa, Emily S. Winn-Deen
Abstract: A biosensor includes a substrate with areas of active receptive material disposed thereon. The receptive material is specific for an analyte of interest. A pattern of the active areas is defined on the substrate by an oxidizing photo-masking process.
Abstract: A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.
Type:
Grant
Filed:
January 18, 2005
Date of Patent:
April 17, 2012
Assignee:
Biocept, Inc.
Inventors:
Zhongliang Tang, Ram S. Bhatt, Pavel Tsinberg
Abstract: The present application provides Ag/AgCl based reference electrodes having an extended lifetime that are suitable for use in long term amperometric sensors. Electrochemical sensors equipped with reference electrodes described herein demonstrate considerable stability and extended lifetime in a variety of conditions.
Type:
Grant
Filed:
June 2, 2008
Date of Patent:
April 10, 2012
Assignee:
Abbott Diabetes Care Inc.
Inventors:
Benjamin J. Feldman, Zenghe Liu, Tianmei Ouyang
Abstract: Disclosed is a peptide-immobilized substrate for measuring a target protein, with which the peptide can have a structure required for being recognized by the target protein, with which the accurate loading amount of the peptide can be attained, and by which a trace amount of the target protein may be measured accurately and simply. The peptide-immobilized substrate for measuring a target protein according to the present invention comprises a chemically synthesized peptide having an expected spatial structure or having a binding ability with the target protein, which peptide can bind with the target protein and is immobilized on the substrate.
Abstract: The present invention relates to the analysis of oxidized phospholipids (OxPL) on apolipoprotein B-100 in patients at high risk or with documented coronary artery disease (CAD) or acute coronary syndromes (ACS) such as unstable angina and acute myocardial infarction or suspected of being at risk for ACS. Such methods are useful for diagnostic purposes and for monitoring the effects of dietary interventions or with drugs such as statins. More particularly, the present invention relates to methods for determining OxPL/apoB ratios as indices of atherosclerosis regression and plaque stability.
Type:
Grant
Filed:
October 5, 2005
Date of Patent:
March 6, 2012
Assignee:
The Regents of the University of California
Abstract: The invention relates to an in vitro method for detecting the formation of endothelins during serious illnesses, especially cardiovascular diseases, inflammations, sepsis and cancer, in whole blood, plasma or serum of a human patient for medical diagnosis. Using this method, relatively long-lasting peptide fragments, especially a C-terminal peptide fragment, of the processed primary prepro- or proendothelins that contain neither the actual biologically active endothelin nor its direct precursor, big endothelin, can be detected.
Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
Type:
Grant
Filed:
April 7, 2006
Date of Patent:
February 21, 2012
Assignee:
Applied Biosystems, LLC
Inventors:
Timothy M. Woudenberg, Michael Albin, Reid B. Kowallis, Yefim Raysberg, Robert P. Ragusa, Emily S. Winn-Deen
Abstract: A biosensor includes a substrate member with a pattern of active areas of receptive material and a pattern of blocking material layers. The receptive material and blocking material are attached to the substrate member with a photo-reactive crosslinking agent activated in a masking process. The receptive material is specific for an analyte of interest.
Type:
Grant
Filed:
August 10, 2010
Date of Patent:
February 7, 2012
Assignee:
Kimberly-Clark Worldwide, Inc.
Inventors:
David Cohen, Rosann Kaylor, Curtis Sayre
Abstract: Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.
Type:
Grant
Filed:
December 22, 2004
Date of Patent:
January 31, 2012
Assignee:
Board of Regents, The University of Texas System
Inventors:
John T. McDevitt, Karri L. Ballard, Pierre N. Floriano, Nick J. Christodoulides, Dean Neikirk, Eric Anslyn, Jason Shear
Abstract: Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.
Type:
Grant
Filed:
December 22, 2004
Date of Patent:
January 24, 2012
Assignee:
Board of Regents, The University of Texas System
Inventors:
John T. McDevitt, Karri L. Ballard, Pierre N. Floriano, Nick J. Christodoulides, Dean Neikirk, Eric Anslyn, Jason Shear
Abstract: The invention provides devices, test kits and methods for removing target agents from a sample. The device contains one or more porous matrices having pore sizes larger than 10 ?m, and a plurality of particles impregnated therein. The target agents attach the device and are removed from the sample.
Type:
Grant
Filed:
June 9, 2005
Date of Patent:
January 24, 2012
Assignee:
Pathogen Removal and Diagnostic Technologies Inc.
Abstract: A homogeneous immunoassay method and system for quantitative determination of total immunoglobulin E and specific antibody levels to a plurality of allergens, in which a relatively small sampling of blood is required. The method utilizes relatively small microparticles in aqueous suspension. The immunoassay procedure is an immunometric sandwich procedure preferably utilizing biotin-streptavidin signal amplification techniques and R-phycoerytherin fluorescent labels.
Abstract: The invention provides a method of depleting anti-MHC antibodies in a sample comprising contacting said sample with one or more recombinant MHC molecules or functionally equivalent variants, derivatives or fragments thereof and removing at least the recombinant MHC molecules to which antibodies to said recombinant MHC molecules contained within the sample have bound. This method allows the depletion of one or more specific MHC particularly HLA allele antibodies from a sample.
Type:
Grant
Filed:
July 22, 2003
Date of Patent:
January 3, 2012
Assignees:
Oxford Radcliffe Hospital NHS Trust, Guy's & St. Thomas' Hospital NHS Trust, King's College London
Inventors:
Martin C. N. M. Barnardo, Andrea W. Harmer, Michael Bunce, Robert W. Vaughan, Kenneth I. Welsh
Abstract: Methods that permit the rapid release of one or more analytes from head or body hair or other keratinized structures of an individual (who may previously have ingested one or more of the analytes) are provided. The methods can include contacting the keratinized structure with a reducing agent but not with a proteolytic agent. The methods can further include identification and quantification of the one or more analytes by known analytical techniques such as immunoassays. The described methods do not damage the analyte and do not cause harmful effects on a subsequently-used analyte detection probe (e.g., an antibody).
Type:
Grant
Filed:
April 29, 2008
Date of Patent:
December 27, 2011
Assignee:
Psychemedics Corporation
Inventors:
Virginia Hill, Mohammad Atefi, Michael I. Schaffer
Abstract: Described herein are methods for identifying a subject at risk of progression of heart failure. In some embodiments, the level of galectin-3 in a biological sample from a human subject may be measured and compared to a standard level indicative of risk of progression of heart failure, wherein an elevated level of galectin-3 in the sample indicates a risk of progression of heart failure.