Patents Examined by Richard G Hutson
  • Patent number: 10479981
    Abstract: The present invention relates to polypeptide variants and methods for obtaining variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: October 14, 2016
    Date of Patent: November 19, 2019
    Assignee: Novozymes A/S
    Inventors: Lars Henrik Oestergaard, Gernot J. Abel, Dorte Marie Koefoed Klitgaard, Annette Helle Johansen
  • Patent number: 10465219
    Abstract: The usefulness of ?-galactosidases derived from Bacillus circulans is further enhanced. A modified ?-galactosidase in which one or more amino acids selected from the group consisting of proline 182 (P182), tyrosine 187 (Y187), serine 188 (S188), tryptophan 405 (W405), alanine 406 (A406), glutamine 407 (Q407), tyrosine 449 (Y449), threonine 483 (T483), serine 512 (S512), serine 531 (S531), threonine 533 (T533), serine 534 (S534), asparagine 550 (N550), glutamine 551 (Q551), tryptophan 593 (W593), tyrosine 598 (Y598), proline 602 (P602), proline 604 (P604), tyrosine 609 (Y609), lysine 612 (K612), and tyrosine 615 (Y615), or an amino acid(s) corresponding thereto, has/have been substituted by other amino acid in a ?-galactosidase consisting of the amino acid sequence of any of SEQ ID NOs. 1 to 4 or an amino acid sequence having 90% or more identity to the amino acid sequence set forth in any of SEQ ID NOs. 1 to 4.
    Type: Grant
    Filed: September 26, 2014
    Date of Patent: November 5, 2019
    Assignee: AMANO ENZYME INC.
    Inventors: Genichi Nakamura, Yuko Kuritani, Satoru Ishihara, Tetsuya Takahashi
  • Patent number: 10457968
    Abstract: The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
    Type: Grant
    Filed: November 3, 2009
    Date of Patent: October 29, 2019
    Assignee: Kapa Biosystems, Inc.
    Inventors: William Bourn, Maryke Appel, Gavin Rush, John Foskett, Paul McEwan
  • Patent number: 10443048
    Abstract: A DNA polymerase composition for amplifying nucleic acids can be tolerant of extreme conditions.
    Type: Grant
    Filed: April 10, 2015
    Date of Patent: October 15, 2019
    Assignee: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY
    Inventors: Samir M. Hamdan, Masateru Takahashi
  • Patent number: 10435675
    Abstract: A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: October 8, 2019
    Assignee: Thermo Fisher Scientific Baltics UAB
    Inventors: Arunas Lagunavicius, Arvydas Janulaitis
  • Patent number: 10435680
    Abstract: A thermostable cellobiohydrolase, having a cellulose-binding motif domain including (A1) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1, (B1) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having a cellulose-binding function, or (C1) a polypeptide including an amino acid sequence having 70% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having a cellulose-binding function, and also having a cellobiohydrolase catalytic domain, wherein the thermostable cellobiohydrolase exhibits hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 95° C. and pH 5.5.
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: October 8, 2019
    Assignee: Honda Motor Co., Ltd.
    Inventors: Jiro Okuma, Yoshitsugu Hirose, Migiwa Suda, Asuka Yamaguchi, Yasuhiro Kondo, Masaru Sato, Daisuke Shibata
  • Patent number: 10426821
    Abstract: A method of treating an inflammatory disease is disclosed. The method comprises administering to the subject a therapeutically effective amount of a polypeptide comprising a pro-domain of TNF-alpha converting enzyme (TACE), said polypeptide being devoid of a catalytic domain of said TACE, said polypeptide comprising a modification at a site selected from the group consisting of R58, R56 and K57 which renders said polypeptide resistant to furin degradation said polypeptide being capable of downregulating an activity of TACE, thereby treating the inflammatory disease.
    Type: Grant
    Filed: June 29, 2017
    Date of Patent: October 1, 2019
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Irit Sagi, Eitan Wong, Ran Afik
  • Patent number: 10412977
    Abstract: The present invention relates to methods and uses of at least one bacterial amylase in poultry feed to improve the nutritional value of the feed. The invention also relates to poultry feed and poultry feed additives comprising at least one bacterial amylase and at least one vitamin and/or mineral which improve the nutritional value of the feed.
    Type: Grant
    Filed: December 1, 2014
    Date of Patent: September 17, 2019
    Assignees: Novozymes A/S, DSM IP Assets B.V.
    Inventors: Inge Knap, Jose-Octavio Sorbara, Rafael Gustavo Hermes
  • Patent number: 10392637
    Abstract: The disclosure relates to a recombinant microorganism engineered to express an enzyme which catalyzes the conversion of a primary amine and an acyl thioester to a fatty amide. The disclosure further encompasses a method of producing a fatty amide by culturing the recombinant microorganism in the presence of a carbon source.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: August 27, 2019
    Assignee: REG Life Sciences, LLC
    Inventors: Jason J. Lutes, Stephen B. Del Cardayre
  • Patent number: 10377999
    Abstract: The present invention relates to mixtures comprising a polypeptide or a plurality of polypeptides having biomass-degrading activity that is solubilized from an inclusion body, and retaining biomass-degrading activity, and methods for producing and using the same. The invention described herein provides methods for increasing the yield of recombinant protein with biomass-degrading activity that can be isolated from host cells.
    Type: Grant
    Filed: September 25, 2015
    Date of Patent: August 13, 2019
    Assignee: XYLECO, INC.
    Inventors: Marshall Medoff, Natasha Kreder, James J. Lynch, Sean Landry, Aiichiro Yoshida, Desiree Pangilinan, Thomas Craig Masterman
  • Patent number: 10364420
    Abstract: Covalently-linked DNA polymerases are provided.
    Type: Grant
    Filed: December 6, 2016
    Date of Patent: July 30, 2019
    Assignee: BIO-RAD LABORATORIES, INC
    Inventor: Xiao-Song Gong
  • Patent number: 10357047
    Abstract: An Arthrobacter gandavensis strain having an activity against Clostridium perfringens selected from the strains AP1 filed with DSMZ on Feb. 19, 2014 under the number DSM 28444, AP2 filed with DSMZ on Feb. 19, 2014 under the number DSM 28445, AP3 filed with DSMZ on Feb. 19, 2014 under the number DSM 28446 or AP4 filed with DSMZ on Feb. 19, 2014 under the number DSM 28447.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: July 23, 2019
    Assignee: ADISSEO FRANCE S.A.S.
    Inventors: Ange Pujol, Michel Fons, Caroline Mirande, Estelle Devillard, Lamya Rhayat
  • Patent number: 10351831
    Abstract: Fusion polypeptides having a heterologous 5?-3? exonuclease domain linked to a polymerase that does not naturally have 5?-3? exonuclease activity, as well as methods of their use are provided. Other aspects are also disclosed.
    Type: Grant
    Filed: November 1, 2017
    Date of Patent: July 16, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yan Wang, Man Cheng
  • Patent number: 10344268
    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: July 9, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Daniel Mazur, Peter Vander Horn, Eileen Tozer, Sihong Chen, Guobin Luo, Joshua Shirley, Kevin Heinemann
  • Patent number: 10336991
    Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: July 2, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Marian Peris, Michael Phelan, Barnett Rosenblum, Stephen Hendricks
  • Patent number: 10329545
    Abstract: Presented herein are methods and compositions for thermostable DNA polymerases that may be used to improve the PCR process and to improve the results obtained when using a thermostable DNA polymerase in other recombinant techniques such as DNA sequencing, nick-translation, and reverse transcription.
    Type: Grant
    Filed: November 13, 2015
    Date of Patent: June 25, 2019
    Assignee: ILLUMINA, INC.
    Inventors: Misha Golynskiy, Molly He, Michael Previte, BeiBei Wang, Sergio Peisajovich
  • Patent number: 10329541
    Abstract: The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a ?5 and/or ?6 desaturase to the substrate specificity of a ?4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the ?5 and/or ?6 desaturase and (ii) the ?4 desaturase; and replacing in the amino acid sequence of the mentioned ?5 and/or ?6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the ?5 and/or ?6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the ?4 desaturase, thereby converting the substrate specificity of the ?5 and/or ?6 desaturase to the substrate specificity of the ?4 desaturase.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: June 25, 2019
    Assignees: BASF PLANT SCIENCE COMPANY GMBH, BIORIGINAL FOOD & SCIENCE CORP.
    Inventors: Toralf Senger, Patricia Vrinten, Ze Long Lim
  • Patent number: 10301604
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
    Type: Grant
    Filed: September 13, 2017
    Date of Patent: May 28, 2019
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Yougen Li, Mark S. Payne, Jared B. Parker, Slavko Kralj, Veli Alkan, Richard R. Bott, Robert Dicosimo, Qiong Cheng, Ellen D. Semke, Susan Marie Hennessey
  • Patent number: 10301656
    Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.
    Type: Grant
    Filed: December 29, 2015
    Date of Patent: May 28, 2019
    Assignee: ENI S.p.A.
    Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
  • Patent number: 10301661
    Abstract: The present invention relates to an isolated DNA molecule encoding a fagopyritol synthase. A method for producing a fagopyritol, an insulin mediator, an insulin mediator analog, an insulin mediator homolog, or an insulin mediator inhibitor is also described. The method includes providing a fagopyritol synthase, providing a substrate comprising a galactosyl donor and a galactosyl acceptor, and combining the fagopyritol synthase with the substrate under conditions effective produce a fagopyritol, an insulin mediator, an insulin mediator analog, an insulin mediator homolog, or an insulin mediator inhibitor.
    Type: Grant
    Filed: February 14, 2018
    Date of Patent: May 28, 2019
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Ralph L. Obendorf, Takashi Ueda