Patents Examined by Richard G Hutson
  • Patent number: 10377999
    Abstract: The present invention relates to mixtures comprising a polypeptide or a plurality of polypeptides having biomass-degrading activity that is solubilized from an inclusion body, and retaining biomass-degrading activity, and methods for producing and using the same. The invention described herein provides methods for increasing the yield of recombinant protein with biomass-degrading activity that can be isolated from host cells.
    Type: Grant
    Filed: September 25, 2015
    Date of Patent: August 13, 2019
    Assignee: XYLECO, INC.
    Inventors: Marshall Medoff, Natasha Kreder, James J. Lynch, Sean Landry, Aiichiro Yoshida, Desiree Pangilinan, Thomas Craig Masterman
  • Patent number: 10364420
    Abstract: Covalently-linked DNA polymerases are provided.
    Type: Grant
    Filed: December 6, 2016
    Date of Patent: July 30, 2019
    Assignee: BIO-RAD LABORATORIES, INC
    Inventor: Xiao-Song Gong
  • Patent number: 10357047
    Abstract: An Arthrobacter gandavensis strain having an activity against Clostridium perfringens selected from the strains AP1 filed with DSMZ on Feb. 19, 2014 under the number DSM 28444, AP2 filed with DSMZ on Feb. 19, 2014 under the number DSM 28445, AP3 filed with DSMZ on Feb. 19, 2014 under the number DSM 28446 or AP4 filed with DSMZ on Feb. 19, 2014 under the number DSM 28447.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: July 23, 2019
    Assignee: ADISSEO FRANCE S.A.S.
    Inventors: Ange Pujol, Michel Fons, Caroline Mirande, Estelle Devillard, Lamya Rhayat
  • Patent number: 10351831
    Abstract: Fusion polypeptides having a heterologous 5?-3? exonuclease domain linked to a polymerase that does not naturally have 5?-3? exonuclease activity, as well as methods of their use are provided. Other aspects are also disclosed.
    Type: Grant
    Filed: November 1, 2017
    Date of Patent: July 16, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yan Wang, Man Cheng
  • Patent number: 10344268
    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: July 9, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Daniel Mazur, Peter Vander Horn, Eileen Tozer, Sihong Chen, Guobin Luo, Joshua Shirley, Kevin Heinemann
  • Patent number: 10336991
    Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: July 2, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Marian Peris, Michael Phelan, Barnett Rosenblum, Stephen Hendricks
  • Patent number: 10329545
    Abstract: Presented herein are methods and compositions for thermostable DNA polymerases that may be used to improve the PCR process and to improve the results obtained when using a thermostable DNA polymerase in other recombinant techniques such as DNA sequencing, nick-translation, and reverse transcription.
    Type: Grant
    Filed: November 13, 2015
    Date of Patent: June 25, 2019
    Assignee: ILLUMINA, INC.
    Inventors: Misha Golynskiy, Molly He, Michael Previte, BeiBei Wang, Sergio Peisajovich
  • Patent number: 10329541
    Abstract: The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a ?5 and/or ?6 desaturase to the substrate specificity of a ?4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the ?5 and/or ?6 desaturase and (ii) the ?4 desaturase; and replacing in the amino acid sequence of the mentioned ?5 and/or ?6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the ?5 and/or ?6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the ?4 desaturase, thereby converting the substrate specificity of the ?5 and/or ?6 desaturase to the substrate specificity of the ?4 desaturase.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: June 25, 2019
    Assignees: BASF PLANT SCIENCE COMPANY GMBH, BIORIGINAL FOOD & SCIENCE CORP.
    Inventors: Toralf Senger, Patricia Vrinten, Ze Long Lim
  • Patent number: 10301606
    Abstract: A DNA polymerase, having an amino acid sequence represented by SEQ ID NO:2, or a derivative of the amino acid sequence by substitution, deletion, or addition of at least one amino acid residue. The DNA polymerase is a hybrid DNA polymerase prepared by inserting a thioredoxin binding domain (TBD) of bacteriophage T7 DNA polymerase into a DNA polymerase I (Sau) of Staphylococcus aureus. A method for preparing the DNA polymerase includes: 1) determining a corresponding position and a target substitution sequence in Sau protein for the TBD of the bacteriophage T7 DNA polymerase; 2) devising and synthesizing a primer according to a gene sequence of Sau and a sequence TBD published by GenBank; 3) cloning the Sau-TBD segment acquired in (2) to an expression vector pTrc99A to construct a recombinant vector pTrc99A-Sau-TBD; and 4) transforming Escherichia coli by the recombinant vector pTrc99A-Sau-TBD and inducing protein expression.
    Type: Grant
    Filed: June 13, 2018
    Date of Patent: May 28, 2019
    Assignee: Hangzhou Zhongee Bio-Sci & Tech. Co., Ltd.
    Inventors: Qi Cheng, Bing Zhai, Joseph Chow, Xianzhen Li, Guoxian Liu
  • Patent number: 10301604
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
    Type: Grant
    Filed: September 13, 2017
    Date of Patent: May 28, 2019
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Yougen Li, Mark S. Payne, Jared B. Parker, Slavko Kralj, Veli Alkan, Richard R. Bott, Robert Dicosimo, Qiong Cheng, Ellen D. Semke, Susan Marie Hennessey
  • Patent number: 10301661
    Abstract: The present invention relates to an isolated DNA molecule encoding a fagopyritol synthase. A method for producing a fagopyritol, an insulin mediator, an insulin mediator analog, an insulin mediator homolog, or an insulin mediator inhibitor is also described. The method includes providing a fagopyritol synthase, providing a substrate comprising a galactosyl donor and a galactosyl acceptor, and combining the fagopyritol synthase with the substrate under conditions effective produce a fagopyritol, an insulin mediator, an insulin mediator analog, an insulin mediator homolog, or an insulin mediator inhibitor.
    Type: Grant
    Filed: February 14, 2018
    Date of Patent: May 28, 2019
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Ralph L. Obendorf, Takashi Ueda
  • Patent number: 10301656
    Abstract: The present invention concerns an oleaginous yeast variant of the species Rhodosporidium azoricum characterized by higher biomass yields and intra-cellular lipid accumulation useful for the production of bio-fuels higher, in determined conditions, with respect to the wild type strain of the same species. Furthermore, the invention concerns a method through which said oleaginous yeast variant of the species Rhodosporidium azoricum was obtained. The invention further concerns the lipid production by means of said variant strain of oleaginous yeast of the species Rhodosporidium azoricum.
    Type: Grant
    Filed: December 29, 2015
    Date of Patent: May 28, 2019
    Assignee: ENI S.p.A.
    Inventors: Giuliana Franzosi, Daniela Cucchetti, Daniele Bianchi, Silvia Galafassi, Concetta Compagno
  • Patent number: 10287560
    Abstract: Presented herein are recombinases for improved recombinase-mediated amplification of nucleic acids, such as a PCR-library having single-stranded adapter regions, on a patterned flow cell surface for improved cluster amplifications, as well as methods and kits using the same.
    Type: Grant
    Filed: March 28, 2017
    Date of Patent: May 14, 2019
    Assignee: ILLUMINA, INC.
    Inventor: Matthew William Kellinger
  • Patent number: 10280411
    Abstract: Provided are compositions comprising recombinant polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for sequencing RNA or RNA/DNA templates. Polymerases that topologically encircle the template nucleic acid are provided. Also provided are methods of using such polymerases to make a DNA or to sequence a template comprising RNA.
    Type: Grant
    Filed: October 26, 2016
    Date of Patent: May 7, 2019
    Assignee: Pacific Biosciences of California, In.c
    Inventors: Satwik Kamtekar, Jeremiah Hanes, Erik Miller
  • Patent number: 10266837
    Abstract: The present invention relates to the field of plant molecular biology. More particularly, the present invention relates to the isolation of nucleic acids encoding terpene synthases (TPSs), including a novel, multifunctional TPS identified herein as CoTPS2.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: April 23, 2019
    Assignee: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: In-cheol Jang, Mi Jung Kim
  • Patent number: 10233218
    Abstract: A recombinant polypeptide is described which comprises at least one PUF RNA-binding domain capable of specifically binding to a cytosine RNA base.
    Type: Grant
    Filed: January 25, 2017
    Date of Patent: March 19, 2019
    Assignee: The University of Western Australia
    Inventors: Aleksandra Filipovska, Oliver Rackham
  • Patent number: 10233468
    Abstract: An object of the present invention is to provide a microorganism strain that accumulates a high molecular weight PHA, and a PHA production method using the microorganism. The present invention provides a method for producing a PHA copolymer, which includes culturing a microorganism, wherein at least a portion of either of the following genes (a) and (b) of the microorganism has been altered by substitution, deletion, insertion, and/or addition to reduce or eliminate the activity of a PHA degrading enzyme encoded by the gene: (a) a PHA degrading enzyme gene encoding the amino acid sequence of SEQ ID NO:2 in the sequence listing; and (b) a gene encoding a polypeptide having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 in the sequence listing and having PHA degrading enzyme activity.
    Type: Grant
    Filed: October 22, 2013
    Date of Patent: March 19, 2019
    Assignee: KANEKA CORPORATION
    Inventors: Hisashi Arikawa, Shunsuke Sato, Keiji Matsumoto
  • Patent number: 10220098
    Abstract: Disclosed are synthetic polypeptides modeled after NspI nucleoporin which are useful for forming hydrogels characterized by selective permeability. The polypeptides and hydrogels formed from them include phenylalanine-glycine (FG) repeats, which are believed to participate in the selectivity of the nuclear pore complex. Also disclosed are filtering devices, drug delivery devices, and methods of separating or selectively filtering macromolecules using the hydrogels.
    Type: Grant
    Filed: June 19, 2015
    Date of Patent: March 5, 2019
    Assignee: Massachusetts Institute of Technology
    Inventors: Minkyu Kim, Bradley D. Olsen
  • Patent number: 10174355
    Abstract: The present invention relates to a uridine diphosphate (UDP)-glycosyltransferase protein which has glycosylation activity for a hydroxyl group at the C-20 position of a protopanaxadiol (PPD)- or protopanaxatriol (PPT)-type ginsenoside, and a method for glycosylation of UDP using the same.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: January 8, 2019
    Assignees: Korea Advanced Institute of Science and Technology, Intelligent Synthetic Biology Center
    Inventors: Sun Chang Kim, Gil Tsu Choi, Suk Chae Jung, Myung Keun Park, Woo Hyun Kim, Soo Hwan Lim, Wan Taek Im
  • Patent number: 10167455
    Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: January 1, 2019
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Satwik Kamtekar, Lei Jia, Robin Emig, Erik Miller, Walter H. Lee