Patents Examined by Richard G Hutson
  • Recombinant nucleoside-specific ribonuclease and method of producing and using same
    Patent number: 11535834
    Abstract: A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
    Type: Grant
    Filed: November 12, 2019
    Date of Patent: December 27, 2022
    Assignee: University of Cincinnati
    Inventors: Sarah Venus, Balasubrahmanyam Addepalli, Nicholas Paul Lesner, Patrick Alan Limbach
  • Dynamic control of colocalization of proteins
    Patent number: 11530278
    Abstract: The present invention provides a method for controlling colocalization of two or more proteins in a cell. The method comprises expressing the proteins, scaffold RNA molecules having binding motifs for the proteins, and a trigger RNA molecule in the cell. In the presence of the trigger RNA molecule, a scaffold may be assembled (ON) by the scaffold RNA molecules via hybridization such that the proteins may be colocalized; or disassembled (OFF) such that the proteins may be separated and not colocalized. The proteins may provide a biological activity when colocalized or not colocalized.
    Type: Grant
    Filed: January 24, 2020
    Date of Patent: December 20, 2022
    Assignee: University of Delaware
    Inventors: Wilfred Chen, Alexander A. Mitkas
  • DPO4 polymerase variants with improved accuracy
    Patent number: 11530392
    Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and accurate incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.
    Type: Grant
    Filed: December 10, 2018
    Date of Patent: December 20, 2022
    Assignee: Stratos Genomics, Inc.
    Inventors: Mark Kokoris, Marc Prindle, Gregory Scott Thiessen, Alexander Isaac Lehmann, Drew Goodman, Aaron Jacobs, John Chase
  • Detergent composition comprising a DNase
    Patent number: 11518985
    Abstract: The present invention relates to new polypeptides, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides. The present invention also relates to detergent composition comprising polypeptides, a laundering method and the use of polypeptides.
    Type: Grant
    Filed: January 26, 2021
    Date of Patent: December 6, 2022
    Assignee: Novozymes A/S
    Inventors: Klaus Gori, Henrik Marcus Geertz-Hansen, Jesper Salomon, Thomas Holberg Blicher, Mary Ann Stringer, Nikolaj Spodsberg, Tianqi Sun, Morten Gjermansen
  • Modified thermoccocus polymerases
    Patent number: 11512295
    Abstract: Provided herein are modified Archaeal family B polymerases derived from the Archaeal microorganism Thermococcus sp. EP1 that exhibit improved incorporation of nucleotide analogues utilized in DNA sequences.
    Type: Grant
    Filed: September 10, 2020
    Date of Patent: November 29, 2022
    Assignee: Singular Genomics Systems, Inc.
    Inventors: Souad Naji, Carl W. Fuller, Eli N. Glezer, Andrew Spaventa
  • Modified polymerases for improved incorporation of nucleotide analogues
    Patent number: 11473067
    Abstract: The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3? position of the sugar moiety that are larger in size than the naturally occurring 3? hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
    Type: Grant
    Filed: August 24, 2021
    Date of Patent: October 18, 2022
    Assignee: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Geoffrey Paul Smith, David Mark Dunstan Bailey, Raquel Maria Sanches-Kuiper, Harold Swerdlow, David James Earnshaw
  • CASZ compositions and methods of use
    Patent number: 11453866
    Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).
    Type: Grant
    Filed: June 9, 2020
    Date of Patent: September 27, 2022
    Assignee: The Regents of the University of California
    Inventors: Jennifer A. Doudna, David Burstein, Janice S. Chen, Lucas B. Harrington, David Paez-Espino, Jillian F. Banfield
  • Polymerase compositions and methods
    Patent number: 11453909
    Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: September 27, 2022
    Assignee: Life Technologies Corporation
    Inventors: Stephen P. Hendricks, Michael Phelan, Marian Peris, Cheng-Yao Chen, Daniel Mazur, Xinzhan Peng, Amy Castillo
  • Omega-hydroxylase-related fusion polypeptides with improved properties
    Patent number: 11441130
    Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
    Type: Grant
    Filed: June 16, 2015
    Date of Patent: September 13, 2022
    Assignee: Genomatica, Inc.
    Inventors: Baolong Zhu, Andreas W. Schirmer, Cindy Chang
  • CasZ compositions and methods of use
    Patent number: 11441137
    Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).
    Type: Grant
    Filed: May 27, 2020
    Date of Patent: September 13, 2022
    Assignee: The Regents of the University of California
    Inventors: Jennifer A. Doudna, David Burstein, Janice S. Chen, Lucas B. Harrington, David Paez-Espino, Jillian F. Banfield
  • ?-Amylase variants
    Patent number: 11441139
    Abstract: Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: September 13, 2022
    Assignee: DANISCO US INC (157111)
    Inventors: Jonathan K. Lassila, Sandra W. Ramer
  • Glucose oxidase GOD mutant and gene and application thereof
    Patent number: 11434473
    Abstract: The present invention takes the glucose oxidase GOD from Aspergillus niger as the mutation template to obtain the glucose oxidase GOD mutants with improved catalytic efficiency and thermal stability by site directed mutagenesis. The specific activity of the mutant of the present invention is 66% higher than that of the wild type GOD; the enzyme activity of the mutant of the present invention is 13.6 times higher than that of the wild type after being treated at 70° C. for 10 min; and the enzyme activity of the mutant of the present invention is 29.4 times higher than that of the wild type after being treated at 80° C. for 2 min.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: September 6, 2022
    Inventors: Bin Yao, Huiying Luo, Tao Tu, Huoqing Huang, Xiaoyun Su, Yaru Wang, Yingguo Bai, Yuan Wang, Kun Meng
  • Omega-hydroxylase-related fusion polypeptides with improved properties
    Patent number: 11421206
    Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
    Type: Grant
    Filed: October 23, 2019
    Date of Patent: August 23, 2022
    Assignee: Genomatica, Inc.
    Inventors: Baolong Zhu, Andreas W. Schirmer, Cindy Chang
  • Genetically modified microorganisms having improved tolerance towards L-serine
    Patent number: 11407976
    Abstract: The present invention generally relates to the microbiological industry, and specifically to the production of L-serine or L-serine derivatives using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes involved in the degradation of L-serine is attenuated, such as by inactivation, which makes them particularly suitable for the production of L-serine at higher yield. The present invention also provides means by which the microorganism, and more particularly a bacterium, can be made tolerant towards higher concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms.
    Type: Grant
    Filed: October 6, 2020
    Date of Patent: August 9, 2022
    Assignee: CysBio ApS
    Inventors: Hemanshu Mundhada, Alex Toftgaard Nielsen
  • Compositions and methods for identifying RNA binding polypeptide targets
    Patent number: 11401514
    Abstract: The present invention features compositions comprising fusion polypeptides comprising an RNA binding polypeptide operationally linked to an RNA modifying enzyme (e.g., adenosine deaminase, cytidine deaminase), and methods of use therefore.
    Type: Grant
    Filed: September 29, 2016
    Date of Patent: August 2, 2022
    Assignee: BRANDEIS UNIVERSITY
    Inventors: Michael Rosbash, Aoife McMahon, Weijin Xu, Hua Jin
  • Methods for genomic integration in pichia and other host cells
    Patent number: 11390874
    Abstract: The present invention provides high efficiency targeted and marker-less single, double, triple, quadruple, and quintuple integrations by using CRISPR in host cells, including Pichia.
    Type: Grant
    Filed: September 12, 2018
    Date of Patent: July 19, 2022
    Assignee: Amyris, Inc.
    Inventor: Hanxiao Jiang
  • Immobilized poly(n)polymerase
    Patent number: 11384375
    Abstract: The present invention relates to an immobilized poly(N)polymerase (PNP), methods of producing said PNP and uses thereof. Further disclosed is an enzyme reactor and kit comprising the PNP for producing polynucleotidylated ribonucleic acid poly(N)RNA)molecules which are useful in gene therapy, immunotherapy, protein replacement therapy and/or vaccination.
    Type: Grant
    Filed: May 2, 2016
    Date of Patent: July 12, 2022
    Assignee: CureVac AG
    Inventors: Tilmann Roos, Benyamin Yazdan Panah, Markus Conzelmann, Veronika Wagner
  • CasZ compositions and methods of use
    Patent number: 11371031
    Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).
    Type: Grant
    Filed: September 25, 2020
    Date of Patent: June 28, 2022
    Assignee: The Regents of the University of California
    Inventors: Jennifer A. Doudna, David Burstein, Janice S. Chen, Lucas B. Harrington, David Paez-Espino, Jillian F. Banfield
  • Thermostable variants of T7 RNA polymerase
    Patent number: 11359184
    Abstract: A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.
    Type: Grant
    Filed: November 11, 2019
    Date of Patent: June 14, 2022
    Assignee: New England Biolabs, Inc.
    Inventors: Jennifer Ong, Vladimir Potapov, Kuo-Chan Hung, Haruichi Asahara, Shaorong Chong, George Tzertzinis
  • Griffithsin mutants
    Patent number: 11339195
    Abstract: The invention provides modified griffithsin polypeptides comprising the amino acid sequence of SEQ ID NO: 1, as well as corresponding nucleic acids, vectors, cells, fusion proteins, constructs, conjugates, and methods of inhibiting viral infection.
    Type: Grant
    Filed: November 27, 2019
    Date of Patent: May 24, 2022
    Inventors: Barry R. O'Keefe, Tinoush Moulaei, Kenneth E. Palmer, Lisa C. Rohan, Joshua L. Fuqua, Lindsay F. Kramzer