Patents Examined by Richard Hutson
  • Nucleic acid modifying enzymes
    Patent number: 8415129
    Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
    Type: Grant
    Filed: March 14, 2011
    Date of Patent: April 9, 2013
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Yan Wang
  • Histone deacetylases, and uses related thereto
    Patent number: 8399233
    Abstract: The present invention concerns the discovery that proteins encoded by a family of genes, termed here HDx-related genes, which are involved in the control of chromatin structure and, thus in transcription and translation. The present invention makes available compositions and methods that can be utilized, for example to control cell proliferation and differentiation in vitro and in vivo.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: March 19, 2013
    Assignee: President and Fellows of Harvard College
    Inventors: Stuart L. Schreiber, Jack Taunton, Christian A. Hassig, Timothy F. Jamison
  • Deoxyketohexose isomerase and method for producing deoxyhexose and derivative thereof using same
    Patent number: 8389248
    Abstract: Providing 1- or 6-deoxy products corresponding to all of aldohexoses, ketohexoses and sugar alcohols, as based on Deoxy-Izumoring, as well as a method for systematically producing those products. A method for producing deoxyketohexose and a derivative thereof using a deoxyketohexose isomerase derived from Pseudomonas cichorii ST-24 (FERM BP-2736), comprising epimerizing 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose at position 3 to produce the individually corresponding 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose as an intended product.
    Type: Grant
    Filed: November 20, 2007
    Date of Patent: March 5, 2013
    Assignees: National University Corporation Kagawa University, Rare Sugar Production Technical Research Laboratories, LLC., Hayashibara Co., Ltd., Matsutani Chemical Industry Co., Ltd.
    Inventors: Ken Izumori, Masaaki Tokuda, George Fleet, Yoshio Tsujisaka, Kei Takeshita, Keiji Tsusaki, Kazuhiro Okuma
  • Compositions with polymerase activity
    Patent number: 8367376
    Abstract: The invention provides novel compositions with polymerase activity and methods of using the compositions.
    Type: Grant
    Filed: June 29, 2009
    Date of Patent: February 5, 2013
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Peter B. Vander Horn, Yan Wang
  • Methods of improving the introduction of DNA into bacterial cells
    Patent number: 8361771
    Abstract: The present invention relates to methods of improving the introduction of DNA into bacterial host cells.
    Type: Grant
    Filed: July 10, 2012
    Date of Patent: January 29, 2013
    Assignee: Novozymes, Inc.
    Inventors: Michael Thomas, Michael Rey
  • Recombinant reverse transcriptases
    Patent number: 8361754
    Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.
    Type: Grant
    Filed: September 28, 2009
    Date of Patent: January 29, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Liangjing Chen, Robert A. Setterquist, Gary Latham
  • Specific lysis of staphylococcal pathogens by bacteriophage phi11 endolysin
    Patent number: 8361772
    Abstract: The Staphylococcus aureus bacteriophage phi11 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and a SH3b cell wall-binding domain. In turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis-causing pathogens, S. aureus and coagulase negative staphylococci (S. chronogenes, S. epidermis, S. hyicus, S. simulans, S. warneri, and S. xylocus), making it a strong antimicrobial protein and an effective candidate for treating multidrug-resistant staphylococci. Lytic activity is maintained at the pH (6.7) and the ‘free’ calcium concentration (3 mM) of milk. Truncated endolysin-derived proteins, containing just the endopeptidase domain, also lyse staphylococci, in the absence of the SH3b-binding domain.
    Type: Grant
    Filed: July 27, 2011
    Date of Patent: January 29, 2013
    Assignee: The United States of America as Represented by the Secretary of Agriculture
    Inventor: David M. Donovan
  • Polypeptides having nucleic acid binding activity
    Patent number: 8349600
    Abstract: Polypeptides having nucleic acid binding activity are provided. Methods of stabilizing a nucleic acid duplex are provided. Methods of promoting the annealing of complementary nucleic acid strands are provided. Methods of increasing the processivity of a DNA polymerase are provided. Methods of enhancing the activity of a nucleic acid modification enzyme are provided. Fusion proteins are provided. Methods of using fusion proteins are provided. Kits are provided.
    Type: Grant
    Filed: January 6, 2006
    Date of Patent: January 8, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Patrick K. Martin, David A. Simpson
  • Polymerase enzymes and reagents for enhanced nucleic acid sequencing
    Patent number: 8343746
    Abstract: Compositions that include DNA polymerases having increased residence times for nucleotide analogues, particularly modified recombinant ?29-type DNA polymerases with such increased residence times, are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA amplification are also provided. Compositions including ?-thiophosphate nucleotide analogues with four or more phosphate groups are described, as are methods for determining the sequence of nucleic acid molecules using such analogues.
    Type: Grant
    Filed: October 22, 2007
    Date of Patent: January 1, 2013
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: David R. Rank, Arek Bibillo, Paul Peluso
  • nucleic acid polymerases
    Patent number: 8318469
    Abstract: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides.
    Type: Grant
    Filed: December 13, 2005
    Date of Patent: November 27, 2012
    Assignee: Applied Biosystems, LLC
    Inventors: James E. Rozzelle, Elena V. Bolchakova
  • DNA polymerase compositions for quantitative PCR and methods thereof
    Patent number: 8283148
    Abstract: The invention relates to the generation and characterization of Archaeal DNA polymerase mutants with deficient 3?-5? exonuclease activity and reduced base analog detection activity. The invention further provides for Archaeal DNA polymerase mutants with deficient 3?-5? exonuclease activity and reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or reverse transcriptase activity. The invention also discloses methods and applications of DNA polymerases with deficient 3?-5? exonuclease activity and reduced base analog detection activity.
    Type: Grant
    Filed: December 12, 2003
    Date of Patent: October 9, 2012
    Assignee: Agilent Technologies, Inc.
    Inventors: Joseph A. Sorge, Reinhold Dietrich Mueller, Gothami Padmabandu, Nick Roelofs, Holly H. Hogrefe
  • Compositions and methods utilizing DNA polymerases
    Patent number: 8268605
    Abstract: The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3? to 5? exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
    Type: Grant
    Filed: June 29, 2001
    Date of Patent: September 18, 2012
    Assignee: Agilent Technologies, Inc.
    Inventors: Joseph A. Sorge, Connie Jo Hansen, Holly Hogrefe
  • Method for isolating and purifying oligodendrocytes and oligodendrocyte progenitor cells
    Patent number: 8263402
    Abstract: The present invention is directed to a method of separating oligodendrocyte cells or progenitor cells thereof from a mixed population of cells. It comprises selecting a promoter which functions only in the oligodendrocyte cells or progenitor cells thereof, introducing a nucleic acid molecule encoding a fluorescent protein under control of that promoter into the mixed population cells, allowing the oligodendrocyte cells or progenitor cells thereof to express the fluorescent protein, and separating the fluorescent cells from the mixed population cells, where the separated cells are the oligodendrocyte cells or progenitor cells thereof. The invention also relates to the isolated and enriched human oligodendrocyte cells or progenitor cells thereof.
    Type: Grant
    Filed: March 31, 1999
    Date of Patent: September 11, 2012
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Steven A. Goldman, Su Wang
  • Hyperthermophilic DNA polymerase and methods of preparation thereof
    Patent number: 8257953
    Abstract: The present invention relates to a hyperthermophilic DNA polymerase and a preparation method thereof. The invention provides a novel hyperthermophilic DNA polymerase isolated from a Thermococcus sp. strain, a functional equivalent thereof, a protein having the amino acid sequence thereof, and a preparation method thereof. The DNA polymerase according to the invention is a DNA polymerase, which is hyperthermophilic and has an elongation ability and fidelity higher than those of prior commercial DNA polymerases. Thus, the DNA polymerase according to the invention will be useful in precision analysis, precision diagnosis, identification and the like, which require accurate PCR.
    Type: Grant
    Filed: October 2, 2006
    Date of Patent: September 4, 2012
    Assignee: Korean Ocean Research & Development Institute
    Inventors: Jung Hyun Lee, Suk Tae Kwon, Sung Gyun Kang, Sang Jin Kim, Jung Ho Hyun, Kae Kyoung Kwon, Yun Jae Kim, Hyun Sook Lee, Seung Seob Bae, Ki Hoon Nam, Jae Kyu Lim, Jung Ho Jeon, Sung Hyun Yang
  • Method for the prevention or reduction of haze in beverages
    Patent number: 8257760
    Abstract: The invention relates to a method for the prevention or reduction of haze in a beverage by the addition of a prolyl-specific and/or alamine specific endoprotease and the new beverages obtainable by the method according to the invention. It also relates to new endoproteases. It also relates to methods as described above wherein auxiliary enzymes are used in combination with the specific endoprotease. Sequence information of a genomic DNA, cDNA as well as protein sequences are provided.
    Type: Grant
    Filed: May 14, 2003
    Date of Patent: September 4, 2012
    Assignee: DSM IP Assets B.V.
    Inventors: Luppo Edens, Michel Lopez
  • Generation of modified polymerases for improved accuracy in single molecule sequencing
    Patent number: 8257954
    Abstract: Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
    Type: Grant
    Filed: March 30, 2009
    Date of Patent: September 4, 2012
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Sonya Clark, Arek Bibillo, Paul Peluso, Fred Christians, Molly He, Insil Park, Harold Lee, Keith Bjornson, Lei Jia, Robin Emig
  • Glycerol-3-phosphate acyltransferase (GPAT) homologs and use thereof
    Patent number: 8247209
    Abstract: The present invention provides novel genes for glycerol-3-phosphate acyltransferase. In exemplary embodiments, the invention provides a nucleic acid comprising the nucleotide sequence shown in SEQ ID NO: 1 or 4 or a fragment thereof.
    Type: Grant
    Filed: June 12, 2008
    Date of Patent: August 21, 2012
    Assignee: Suntory Holdings Limited
    Inventors: Misa Ochiai, Hisanori Tokuda
  • Methods of improving the introduction of DNA into bacterial cells
    Patent number: 8236544
    Abstract: The present invention relates to methods of improving the introduction of DNA into bacterial host cells.
    Type: Grant
    Filed: October 1, 2010
    Date of Patent: August 7, 2012
    Assignee: Novozymes, Inc.
    Inventors: Michael Thomas, Michael Rey
  • Isolated luciferases and the use thereof
    Patent number: 8236540
    Abstract: The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22.
    Type: Grant
    Filed: October 22, 2007
    Date of Patent: August 7, 2012
    Assignee: Bayer Intellectual Property GmbH
    Inventors: Stefan Golz, Bernd Kalthof, Svetlana Markova, Ludmila Frank, Eugene Vysotski
  • Methods of using improved polymerases
    Patent number: 8232078
    Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.
    Type: Grant
    Filed: November 27, 2002
    Date of Patent: July 31, 2012
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yan Wang, Peter Vander Horn, Lei Xi