Patents Examined by Richard Hutson
  • Restriction endonucleases, DNA encoding these endonucleases and methods for identifying new endonucleases with the same or varied specificity
    Patent number: 8227231
    Abstract: Specified restriction endonucleases have been characterized for the first time by their amino acid and DNA sequences. These sequences and those with at least 90% identity thereto have been used as probes in sequence similarity analyses to identify sequence matches in a sequence database that corresponds to novel restriction endonucleases or isoschizomers. The sequence similarity analyses includes selecting a positive sequence match from any sequence producing an expectation value of less than or equal to e-02.
    Type: Grant
    Filed: August 3, 2006
    Date of Patent: July 24, 2012
    Assignee: New England Biolabs, Inc.
    Inventors: Richard D. Morgan, Richard J. Roberts
  • Hybrid and single chain meganucleases and use thereof
    Patent number: 8206965
    Abstract: This patent application relates to hybrid and/or single-chain rare-cutting endonucleases, called meganucleases, which recognize and cleave a specific nucleotide sequence, to polynucleotide sequences encoding for said rare-cutting endonucleases, to a vector comprising one of said polynucleotide sequences, to a cell or animal comprising one of said polynucleotide sequences or said rare-cutting endonucleases, to a process for producing one of said rare-cutting endonucleases and any use of the disclosed products and methods. More particularly, this invention contemplates any use of such rare-cutting endonuclease for genetic engineering and gene therapy.
    Type: Grant
    Filed: March 14, 2003
    Date of Patent: June 26, 2012
    Assignee: Cellectis S.A.
    Inventors: Sylvain Arnould, Patrick Chames, Andre Choulika, Jean-Charles Epinat, Emmanuel Lacroix
  • Nematode phosphoethanolamine N-methyltransferase-like sequences
    Patent number: 8198060
    Abstract: Nucleic acid molecules from nematodes encoding phosphoethanolamine n-methyltransferase polypeptides are described. PEAMT-like polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of PEAMT-like nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.
    Type: Grant
    Filed: October 28, 2008
    Date of Patent: June 12, 2012
    Assignee: Divergence, Inc.
    Inventors: Deryck J. Williams, Merry B. McLaird, Michelle Coutu Hresko, Anita M. Frevert, Ronald E. Worthington, Andrew P. Kloek, Jennifer A. Davila-Aponte, John D. Bradley, Siqun Xu
  • Detection of mutations in a gene associated with resistance to viral infection, OAS1
    Patent number: 8192973
    Abstract: A method for detecting a mutation related to the gene encoding OAS1. This and other disclosed mutations correlate with resistance of humans to viral infection including hepatitis C. Also provided is a therapeutic agent consisting of a protein or polypeptide encoded by the mutated gene, or a polynucleotide encoding the protein or polypeptide. Inhibitors of human OAS1, including antisense oligonucleotides, methods, and compositions specific for human OAS1, are also provided.
    Type: Grant
    Filed: November 2, 2006
    Date of Patent: June 5, 2012
    Assignee: Kineta Two, LLC
    Inventors: Shawn P Iadonato, Charles L Magness, Gary Rosenberg, Christina A Scherer, Thierry Guillaudeux
  • One component and two component DNA Pol III replicases and uses thereof
    Patent number: 8192960
    Abstract: The invention provides one-component and two-component DNA polymerases, as well as kits comprising the same, and methods of using the same for nucleic amplification and nucleic acid sequencing.
    Type: Grant
    Filed: April 7, 2005
    Date of Patent: June 5, 2012
    Assignee: Qiagen North American Holdings
    Inventors: Lars-Erik Peters, Anna Ettinger
  • Determination of ions using ion-sensitive enzymes
    Patent number: 8187831
    Abstract: The invention relates generally to the field of sodium and lithium ion detection. In particular, the invention provides chimeric proteins, nucleic acids encoding chimeric proteins, methods and kits for assaying for sodium ions and for lithium ions in a sample, using inter alia, a 3?(2?),5?-bisphosphate nucleotidase.
    Type: Grant
    Filed: September 19, 2003
    Date of Patent: May 29, 2012
    Assignee: General Atomics
    Inventor: Chong-Sheng Yuan
  • Assays for nucleic acid modifying enzymes
    Patent number: 8182986
    Abstract: Methods and compositions are provided for assaying for enzymes capable of releasing an enzyme donor fragment (ED) conjugated to a nucleic acid strand bonded to a surface. Conveniently, the beads are magnetic allowing segregation of the beads during the determination. Upon addition of enzyme acceptor fragment (EA) and substrate to the assay mixture, the method allows for discrimination between ED free in solution and ED bound to the bead. The complexing of ED and EA provides an active ?-galactosidase enzyme. The method permits the assay of any substance involved in a pathway that can result in a reaction releasing the ED.
    Type: Grant
    Filed: July 12, 2002
    Date of Patent: May 22, 2012
    Assignee: Discoverx
    Inventors: Pyare Khanna, Shu-Ling Cheng
  • Method of producing L-lysine
    Patent number: 8183017
    Abstract: The ability and speed with which a coryneform bacterium can produce L-lysine are improved when the coryneform bacterium contains an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized. This is accomplished by successively enhancing the DNA coding for dihydrodipicolinate reductase, the DNA coding for dihydrodipicolinate synthase, the DNA coding for diaminopimelate decarboxylase, and the DNA coding for diaminopimelate dehydrogenase.
    Type: Grant
    Filed: October 29, 2010
    Date of Patent: May 22, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Seiko Otsuna, Masakazu Sugimoto, Masako Izui, Atsushi Hayakawa, Eiichi Nakano, Masaki Kobayashi, Yasuhiko Yoshihara, Tsuyoshi Nakamatsu
  • Nucleic acids encoding a domain-containing polypeptide
    Patent number: 8153400
    Abstract: The invention pertains to nucleic acids encoding a mutT domain-containing polypeptide, including fragments and biologically functional variants thereof. The invention also pertains to therapeutics and diagnostics involving the foregoing polypeptide and nucleic acids and agents that bind the foregoing polypeptide and nucleic acids. The invention also pertains to the identification of a novel mutT domain in human TrpC7, a polypeptide previously described as a putative calcium ion channel. Accordingly, the invention also pertains to methods and compositions for identifying agents useful modulating mutT domain-mediated calcium or other ion transport in cells expressing a polypeptide comprising a mutT domain and a calcium or other ion channel.
    Type: Grant
    Filed: April 26, 2000
    Date of Patent: April 10, 2012
    Assignee: Beth Israel Deaconess Medical Center, Inc.
    Inventor: Andrew M. Scharenberg
  • Polymerase
    Patent number: 8148126
    Abstract: The invention relates to thermostable polymerases that have polymerase activity temperatures in the range from 90.degree. C. up to 113.degree. C., such as those derived from Pyrolobus fumaria, and to polynucleotides encoding the polymerases In addition, methods of designing new thermostable DNA polymerases and methods of use thereof are also provided. The polymerases have increased activity and stability at increased pH and temperature.
    Type: Grant
    Filed: July 2, 2010
    Date of Patent: April 3, 2012
    Assignee: Verenium Corporation
    Inventors: Walter N. Callen, Eric J. Mathur
  • Mutant phytase polypeptide
    Patent number: 8143045
    Abstract: The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.
    Type: Grant
    Filed: April 3, 2007
    Date of Patent: March 27, 2012
    Assignee: Danisco A/S
    Inventors: Andrei Miasnikov, Vijay Kumar, Oliver Kensch, Klaus Pellangahr, Birgitta Leuthner, Ulrich Kettling, Andre Koltermann
  • Variant sp. phytases having altered properties
    Patent number: 8143046
    Abstract: The present invention relates to variant phytase enzymes having altered properties.
    Type: Grant
    Filed: February 6, 2008
    Date of Patent: March 27, 2012
    Assignee: Danisco US Inc., Genencor Division
    Inventors: Marguerite A. Cervin, Oliver Kensch, Ulrich Kettling, Steven S. Kim, Birgitta Leuthner, Andrei Miasnikov, Michael Ward, Klaus Pellengahr
  • Chondroitin polymerase and DNA encoding the same
    Patent number: 8137941
    Abstract: A chondroitin polymerase having such properties that it transfers GlcUA and GalNAc alternately to a non-reduced terminal of a sugar chain from a GlcUA donor and a GalNAc donor, respectively, and the like; and a process for producing the chondroitin polymerase.
    Type: Grant
    Filed: August 10, 2009
    Date of Patent: March 20, 2012
    Assignee: Seikagaku Corporation
    Inventors: Toshio Ninomiya, Nobuo Sugiura, Koji Kimata
  • Thermostable polymerases from
    Patent number: 8124391
    Abstract: The invention relates to a thermostable polymerase based on thermococcus pacificus; DNA molecules which code for one such polymerase; expression vectors; host cells; methods for producing one such polymerase and the use thereof for polymerising nucleic acid, especially in the polymerase chain reaction.
    Type: Grant
    Filed: October 5, 2001
    Date of Patent: February 28, 2012
    Assignee: Qiagen GmbH
    Inventors: Katja Decker, Dirk Löffert, Jie Kang
  • Polypeptides of strain sp. P203
    Patent number: 8119387
    Abstract: A new strain Bacillus sp. P203 (Bacillus plakortiensis) is disclosed. Isolated mature functional polypeptide which is obtainable from the bacterium strain Bacillus sp. P203 deposited under accession number DSM 17419 are disclosed.
    Type: Grant
    Filed: August 15, 2006
    Date of Patent: February 21, 2012
    Assignee: Novozymes A/S
    Inventors: Preben Nielsen, Reinhard Wilting, Martin Borchert
  • Variant of KpnI restriction endonuclease
    Patent number: 8119382
    Abstract: Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated.
    Type: Grant
    Filed: August 18, 2006
    Date of Patent: February 21, 2012
    Assignees: New England Biolabs, Inc., Indian Institute of Science
    Inventors: Valakunja Nagaraja, Matheshwaran Saravanan, Zhenyu Zhu
  • Method for the prevention or reduction of haze in beverages
    Patent number: 8119171
    Abstract: The invention relates to a method for the prevention or reduction of haze in a beverage by the addition of an prolyl-specific endoprotease and to new beverages obtainable by the method according to the invention. It also relates to new endoproteases. Sequence information of a genomic DNA, cDNA as well as protein sequences.
    Type: Grant
    Filed: December 6, 2001
    Date of Patent: February 21, 2012
    Assignee: DSM IP Assets B.V.
    Inventors: Michel Lopez, Luppo Edens
  • Materials and methods for the generation of fully 2?-modified nucleic acid transcripts
    Patent number: 8105813
    Abstract: Materials and methods are provided for producing aptamer therapeutics having fully modified nucleotide triphosphates incorporated into their sequence.
    Type: Grant
    Filed: June 30, 2006
    Date of Patent: January 31, 2012
    Assignee: Archemix Corp.
    Inventors: John L. Diener, Anthony Dominic Keefe, Kristin Thompson, Chunhua Wang, Shuhao Zhu
  • DNA replication factors
    Patent number: 8105814
    Abstract: A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from Pyrococcus horikoshii, a hyperthermophilic archaeon.
    Type: Grant
    Filed: August 25, 2010
    Date of Patent: January 31, 2012
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Ikuo Matsui, Yuji Urushibata
  • Materials and methods for the generation of transcripts comprising modified nucleotides
    Patent number: 8101385
    Abstract: Materials and methods are provided for producing aptamer therapeutics having modified nucleotide triphosphates incorporated into their sequence.
    Type: Grant
    Filed: December 21, 2007
    Date of Patent: January 24, 2012
    Assignee: Archemix Corp.
    Inventors: Sharon Cload, John L. Diener, Anthony Dominic Keefe, Kristin Thompson