Patents Examined by Richard Hutson
  • Mutations in OAS1 genes
    Patent number: 8951768
    Abstract: Modified amino acid sequences of OAS1 proteins in non-human primates, and genes related thereto, are provided.
    Type: Grant
    Filed: July 11, 2011
    Date of Patent: February 10, 2015
    Assignee: Kineta Two, LLC
    Inventors: Shawn P. Iadonato, Charles L. Magness, P. Campion Fellin, Christina A. Scherer, Tory Hagen, Amy Olson
  • Active surface coupled polymerases
    Patent number: 8936926
    Abstract: Active surface coupled polymerases, surfaces that include such polymerases, and methods of making and using surface-attached polymerases are provided.
    Type: Grant
    Filed: December 21, 2006
    Date of Patent: January 20, 2015
    Assignee: Pacific Biosciences of California
    Inventors: David Hanzel, Jonas Korlach, Paul Peluso, Geoffrey Otto, Thang Pham, David Rank, Stephen Turner
  • I-I derived single-chain meganuclease and uses thereof
    Patent number: 8927247
    Abstract: A new I-CreI derived single-chain meganuclease comprising two domains, each domain comprising a portion of a parent I-CreI monomer which extends at least from the beginning of the first alpha helix to the end of the C-terminal loop and said two domains being joined by a peptidic linker which allows them to fold as a I-CreI dimer that is able to bind and cleave a chimeric DNA target comprising one different half of each parent homodimeric I-CreI meganuclease target sequence. Use of said I-CreI derived single-chain meganuclease for genetic engineering, genome therapy and antiviral therapy.
    Type: Grant
    Filed: February 2, 2009
    Date of Patent: January 6, 2015
    Assignee: Cellectis, S.A.
    Inventor: Sylvestre Grizot
  • Methods for obtaining thermostable enzymes, DNA polymerase I variants from having new catalytic activities, methods for obtaining the same, and applications to the same
    Patent number: 8927699
    Abstract: A thermostable mutant DNA polymerase; a method for obtaining the thernmostable mutant DNA polymerase by identifying a thermostable mutant polypeptide exhibiting enzymatic activity, wherein the thermostable mutant polypeptide is a variant of DNA polymerase I obtained from Thermus aquaticus; a polynucleotide, an expression vector, and a host cell encoding the thermostable mutant DNA polymerase; and a method of performing a reverse transcription polymerase chain reaction (RT-PCR) utilizing the thermostable mutant DNA polymerase, as well as a kit for facilitating the same.
    Type: Grant
    Filed: February 25, 2005
    Date of Patent: January 6, 2015
    Assignees: Institut Pasteur, Centre National de la Recherche Scientifique (CNRS)
    Inventors: Jean-Luc Jestin, Sophie Vichier-Guerre, Stephane Ferris
  • Polymerases for nucleotide analogue incorporation
    Patent number: 8921086
    Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
    Type: Grant
    Filed: December 21, 2006
    Date of Patent: December 30, 2014
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: David K. Hanzel, Geoff Otto, Paul Peluso, Thang Pham, David R. Rank, Paul Mitsis, Fred Christians, Arek Bibillo, Insil Park, Sonya Clark, John Lyle
  • Mammalian alpha-kinase proteins, nucleic acids and diagnostic and therapeutic uses thereof
    Patent number: 8916379
    Abstract: The present invention provides novel mammalian alpha-kinase proteins: melanoma alpha-kinase (MK), heart alpha-kinase (HK), kidney alpha-kinase (KK), skeletal muscle alpha-kinase (SK), and lymphocyte alpha-kinase (LK). In particular, a novel kinase type is herein provided, characterized by the presence of an alpha-kinase catalytic domain and an ion channel domain. Isolated nucleic acids of the alpha-kinases MK, HK, KK, SK and LK are provided. Methods for making the novel alpha-kinases, cells that express the alpha-kinases and methods for treating an animal in need of either increased or decreased activity of the alpha-kinases are provided.
    Type: Grant
    Filed: June 17, 2010
    Date of Patent: December 23, 2014
    Assignee: Rutgers, The State University of New Jersey
    Inventor: Alexey G. Ryazanov
  • Endoglucanase variants
    Patent number: 8906689
    Abstract: The present invention relates to variant endoglucanases having improved thermoactivity, improved thermostability, and improved viscosity reduction activity over wild-type M. thermophila endoglucanase.
    Type: Grant
    Filed: December 20, 2011
    Date of Patent: December 9, 2014
    Assignee: Codexis, Inc.
    Inventors: Xiyun Zhang, Sachin Patil, Jie Yang, Ish Kumar Dhawan
  • Nucleic acid modifying enzymes
    Patent number: 8900846
    Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
    Type: Grant
    Filed: October 18, 2013
    Date of Patent: December 2, 2014
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Yan Wang
  • Nucleic acid modifying enzymes
    Patent number: 8895283
    Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
    Type: Grant
    Filed: March 25, 2013
    Date of Patent: November 25, 2014
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Yan Wang
  • Multiple domain proteins
    Patent number: 8889394
    Abstract: Described herein are methods and compositions for generating and using fusion proteins.
    Type: Grant
    Filed: September 7, 2009
    Date of Patent: November 18, 2014
    Assignee: Empire Technology Development LLC
    Inventor: Sreekanth H. Chalasani
  • Modified polymerases for improved incorporation of nucleotide analogues
    Patent number: 8852910
    Abstract: The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogs bearing substituents at the 3? position of the sugar moiety that are larger in size than the naturally occurring 3? hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
    Type: Grant
    Filed: April 4, 2013
    Date of Patent: October 7, 2014
    Assignee: Illumina Cambridge Limited
    Inventors: Geoffrey Paul Smith, David Mark Dunstan Bailey, Raquel Maria Sanches-Kuiper, Hardold Swerdlow, David James Earnshaw
  • SSB-polymerase fusion proteins
    Patent number: 8828700
    Abstract: Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).
    Type: Grant
    Filed: December 1, 2008
    Date of Patent: September 9, 2014
    Assignee: Life Technologies Corporation
    Inventors: Jun Lee, Robert Potter, David Mandelman
  • Cancer selective auxotrophs
    Patent number: 8822194
    Abstract: Bacteria that are auxotrophic for at least two amino acids found in at least one tumor are effective anti tumor treatments, labeling agents, and vaccines against infection. Improved antitumor effects can also be provided such strains by passage through an appropriate tumor model.
    Type: Grant
    Filed: June 29, 2005
    Date of Patent: September 2, 2014
    Assignee: Anticancer, Inc.
    Inventors: Ming Zhao, Meng Yang
  • Recombinant enzyme with altered feedback sensitivity
    Patent number: 8795990
    Abstract: The present invention relates to the use of recombinant homoserine transsuccinylase enzymes with altered feedback sensitivity (MetA*) and possibly recombinant S-adenosyl methionine synthetase enzymes with reduced activity (MetK*) for the production of methionine, its precursors or derivatives thereof.
    Type: Grant
    Filed: May 12, 2005
    Date of Patent: August 5, 2014
    Assignee: Metabolic Explorer
    Inventors: Gwénaëlle Bestel-Corre, Michel Chateau, Rainer Figge, Céline Raynaud, Philippe Soucaille
  • Compositions and methods utilizing DNA polymerases
    Patent number: 8772006
    Abstract: The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3? to 5? exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
    Type: Grant
    Filed: May 15, 2006
    Date of Patent: July 8, 2014
    Assignee: Agilent Technologies, Inc.
    Inventors: Joseph A. Sorge, Connie Jo Hansen, Holly Hogrefe
  • DNA polymerases with increased 3?-mismatch discrimination
    Patent number: 8765435
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: July 11, 2011
    Date of Patent: July 1, 2014
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Keith Bauer, Fred Reichert, Thomas W. Myers
  • DNA polymerases with increased 3?-mismatch discrimination
    Patent number: 8765436
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: July 11, 2011
    Date of Patent: July 1, 2014
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Keith Bauer, Fred Reichert, Thomas W. Myers
  • DNA polymerases with increased 3?- mismatch discrimination
    Patent number: 8759062
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: June 17, 2011
    Date of Patent: June 24, 2014
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer
  • Mutated DNA polymerases with increased mispairing discrimination
    Patent number: 8759061
    Abstract: The present invention relates to DNA polymerases with a special mutation which have an enhanced mismatch discrimination, the preparation and use thereof. The thermostable DNA polymerases with this mutation are particularly suitable for diagnostic and molecular-biological methods, e.g., allele-specific PCR.
    Type: Grant
    Filed: February 4, 2005
    Date of Patent: June 24, 2014
    Assignee: Rheinische Friedrich-Wilhelms-Universitaet Bonn
    Inventors: Andreas Marx, Daniel Summerer, Nicolas Zackes Rudinger
  • Thermostable reverse transcriptases and uses thereof
    Patent number: 8753845
    Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
    Type: Grant
    Filed: December 28, 2010
    Date of Patent: June 17, 2014
    Assignee: Life Technologies Corporation
    Inventors: Gulshan Dhariwal, Gary Gerard, Jun Lee, Robert Potter, Kim Rosenthal, Michael D. Smith