Abstract: The present disclosure relates to chimeric Tim4 receptors, host cells modified to include chimeric Tim4 receptor molecules, and methods of making and using such receptor molecules and modified cells.

Abstract: The present disclosure relates to a method that includes utilizing a microorganism for the converting of a substrate to an acid contained in a mixture that includes the acid and water, maintaining a pH of the mixture to less than 5, and treating the mixture with a first stream comprising an organic.

Abstract: The present invention belongs to the field of organophosphorus pesticide (OP) detection, and relates to a method for detecting an OP by a microfluidic chip based on a fluorescent sensing film. A porous fluorescent sensing film and the microfluidic chip are first constructed. The fluorescent sensing film is fabricated through layer-by-layer self-assembly of a platinum nanoparticle@oxalate-metal-organic framework (MOF) composite and a porous two-dimensional (2D) nanosheet, and has the functions of specifically detecting OPs and blocking macromolecular interferents. The microfluidic chip includes a sample channel, injection channels, reaction tanks, microfluidic channels, a detection tank, and an optical fiber channel, such that sample pretreatment and detection processes are integrated in the chip.

Abstract: An apparatus includes a portable housing with a cavity, a heater disposed within the cavity of the housing, a temperature buffering device coupled to the heater and disposed within the cavity of the housing, a non-biological skin substitute substrate coupled to the temperature buffering device and disposed within the cavity of the housing, and a carbon dioxide delivery device coupled to the housing. The non-biological skin substrate simulates a surface property of human skin.

Abstract: The disclosure discloses a feruloyl esterase and application thereof, and belongs to the technical field of microorganisms. The disclosure provides a feruloyl esterase with an amino acid sequence shown in SEQ ID NO: 2 and specific enzyme activity as high as 519 U/g. The disclosure provides a recombinant Bacillus subtilis which uses Bacillus subtilis as a host, expresses a gene encoding the feruloyl esterase with an amino acid sequence shown in SEQ ID NO: 2, and can be used for producing the feruloyl esterase with a high yield. The process specifically includes inoculating a fermentation culture medium with the recombinant Bacillus subtilis for fermentation for 14 hours, and the enzyme activity of the feruloyl esterase in a cell disruption supernatant can be as high as 82.53 U/mL.

Abstract: Disclosed are sensors and related methods for measuring enzyme activity in a measurement environment and for quantifying enzyme and/or microbe concentrations in the measurement environment. An enzyme activity sensor includes a degradable conductive trace and a biodegradable target configured to enable the conductive trace, in an initial state, to conduct electrical current between first and second terminal ends via conductive particles. The conductive trace is configured to degrade over time as a result of enzymatic activity that degrades the biodegradable target when the sensor is placed in a measurement environment, the enzymatic activity thereby increasing resistance between the first and second terminal ends.

Abstract: Disclosed are methods of identifying a secretome from a subject cell within a heterogeneous cell population when the subject cell contacts a target cell (e.g. a subject immune cell contacts a target cancer cell) or a stimulatory agent and methods of using the identified secretome to identify cells that are safe and efficacious for cellular therapies, including adoptive CAR T-cell therapies.

Abstract: A method for synthesizing single-stranded DNA, specifically a process for producing single-stranded DNA without base mutations, is provided, by which single-stranded DNA is produced by uracil-specific excision reagent (USER)-mediated self-looping of double-stranded DNA combined with rolling circle replication.

Abstract: Bioreactor systems and controlled operation of bioreactor systems are disclosed herein. The bioreactor systems can include at least one bioreactor chamber, at least one reservoir, a plurality of sensors, and a fluid circuit. The operational methods disclosed herein are directed towards growing cells or tissue while measuring various parameters, and a controlled operation of the various parameters during the operation of the bioreactor systems. The controlled operation of the parameters includes, for example, cell concentration; a rate of flow; a volume; a pH; a temperature; a level of oxygen; a level of carbon dioxide; a level of bicarbonate ion; nutrient compound; and any combination thereof.

Abstract: A granule for detecting occult blood in pet urine including 50-70 parts by weight of an adsorbent, 50-60 parts by weight of a filler, 3-5 parts by weight of an antibacterial agent, 5-10 parts by weight of a deodorant, 2-4 parts by weight of cumene hydroperoxide, and 2-4 parts by weight of an indicator.

Abstract: The present invention relates to a composition comprising Pediococcus pentosaceus, one or more yeast, wherein a weight ratio of Pediococcus pentosaceus:yeast is between 0.01 to 0.5:1, and optionally one or more minerals, sugar and pharmaceutical acceptable excipients, additives and/or adjuvants. The invention also relates to a use of the composition as food supplement or in treatment and/or prevention of a disease in a mammal, such as a human and a use in treatment and/or prevention of food and/or alcohol poisoning in a mammal.

Abstract: The present invention relates to compositions and methods for activating expression from the paternally-inherited allele of UBE3A in human Angelman's Syndrome neurons using viral vector delivery of short hairpin RNAs, ribozymes, and/or microRNAs.

Abstract: The present invention relates to the field of biomedicine, and in particular to an engineered migrasome, a method for preparing the engineered migrasome, a delivery system comprising the engineered migrasome, and a method for preparing the delivery system.

Abstract: Described herein are probiotics, more particularly [to] the probiotic yeast Saccharomyces boulardii. Even more particularly described herein are enhanced probiotic potency S. boulardii. Also described here are mutant alleles useful to develop yeast strains with enhanced production of acetic acid. In addition, described here is the use of such yeast strains for the production of dietary supplements or pharmaceutical compositions to improve gastrointestinal comfort.

Abstract: Compositions and methods for mucosal delivery of agents are provided. The emulsion compositions are intended for administration to a mucosal surface, such as oral, gastrointestinal and nasal mucosa. The emulsion compositions provided contain one or more mucoadhesive proteins and an agent to be delivered. Methods for delivery of agents using the compositions provided herein are also provided.

Abstract: The present application relates to a psicose-6-phosphate phosphatase comprising motif A and motif B, a composition for producing D-psicose comprising the enzyme, and a method for producing D-psicose using the enzyme.

Abstract: The present invention comprises methods and compositions comprising a microalgae consortium. A composition comprises at least one genera of microalgae flocculated by Paenibacillus polymyxa strain, Strain 2, deposited under the Budapest Treaty as ATCC Accession No. PTA-12841. Methods of treating the soil comprise adding a composition of the present invention to soil.

Abstract: The present disclosure provides dimeric antigen receptors (DAR) constructs comprising a heavy chain binding region on one polypeptide chain and a light chain binding region on a separate polypeptide chain. The two polypeptide chains that make up the dimeric antigen receptors can dimerize to form an antigen binding domain. The dimeric antigen receptors have antibody-like properties as they bind specifically to a target antigen. The dimeric antigen receptors can be used for directed cell therapy.

Abstract: Provided is a method of producing monophosphoryl lipid A (MPLA). According to the method, MPLA may be produced with high purity and high purity by using a bacterium producing MPLA.

Abstract: The invention discloses a genetically engineered bacterium in which the gene encoding adenine deaminase on the genome of the bacterium is knocked out or/and the gene encoding the enzyme in the NAD+ anabolic pathway is integrated on the genome of the bacterium. The invention also discloses a construction method of the above-mentioned genetically engineered bacteria. The gene encoding adenine deaminase on the genome of the host strain is knocked out to obtain a strain with high NAD+ yield. Or the expression cassettes of the gene encoding the enzyme in the NAD+ synthesis pathway are constructed separately, and then the enzyme encoding The gene expression cassette is integrated into the genome of the host strain whose gene encoding adenine deaminase is knocked out to construct a strain with high NAD+ production. The application of the above genetically engineered bacteria is disclosed. A method of producing NAD+ is disclosed.