Patents Examined by Robert J Yamasaki
  • Patent number: 12146178
    Abstract: Methods for obtaining a carboxylic acid product from a lactose-containing feedstock include contacting the lactose-containing feedstock and a first mixture of microorganisms in a first bioreactor under anaerobic conditions at a temperature of about 45° C. to about 55° C. and a pH of from about 4 to about 6 for a period of time such that lactic acid is formed; contacting the lactic acid with a second mixture of microorganisms in a second bioreactor under anaerobic conditions at a temperature of about 25° C. to about 35° C. and a pH of from about 4 to about 6 for a period of time such that the lactic acid is converted to one or more C3-C12 carboxylic acid products; and isolating the one or more C3-C12 carboxylic acid products. The lactose-containing feedstock has a pH greater than 4.5.
    Type: Grant
    Filed: February 26, 2020
    Date of Patent: November 19, 2024
    Assignee: Capro-X, Inc.
    Inventor: Juan J. Guzman, Jr.
  • Patent number: 12138319
    Abstract: A human chimeric protein(1) is described, expressed by a viral vector (2) designed for treating patients affected by genetic disorders, composed of a first cDNA sequence [SEQ. 2] of a N-terminal extracellular portion of a human receptor (4) of low-density lipoproteins (5) (hLDLR), fused with a second cDNA sequence [SEQ. 3] of the human transferrin (7) (hTf).
    Type: Grant
    Filed: January 15, 2020
    Date of Patent: November 12, 2024
    Inventors: Lucio Pastore, Eleonora Leggiero
  • Patent number: 12130296
    Abstract: The present invention relates to method for the direct detection and/or quantification of at least one compound with a molecular weight of at least 200, wherein the compound to be detected and/or quantified is a chemically complex molecule, wherein said chemically complex molecule is substituted with at least two groups R, wherein each R group means independently —OH, —OP(O)(OH)2 or —P(O)(OH)2, with the proviso that at least two R are independently selected from —P(O)(OH)2 and —OP(O)(OH)2, wherein the compound or compounds to be detected and/or quantified are within a biological matrix, wherein said biological matrix is a biological fluid, a biological tissue, stomach contents, intestine contents, stool sample or a culture cells, wherein the method comprises performing a chromatography and identifying the retention time and/or the intensity of the signal by means of a mass or radioactivity detector.
    Type: Grant
    Filed: October 28, 2021
    Date of Patent: October 29, 2024
    Assignee: SANIFIT THERAPEUTICS S.A.
    Inventors: Joan Perello Bestard, Ciriaco Maraschiello De Zuani, Irene Lentheric, Paula Mendoza De Las Heras, Fernando Tur Espinosa, Eva Tur Tur, Maximo Encabo Alarcon, Eva Martin Becerra, Maria de Mar Benito Amengual, Bernat Isern Amengual
  • Patent number: 12116607
    Abstract: The present invention relates to a method for producing a domain antibody in a yeast, wherein the formation of disulfide bridges in the domain antibody is promoted. The method encompasses the addition of oxidizing agents, preferably oxidizing metal ions, preferably one or more selected from Cu2+, Fe2+, Fe3+ and Zn2+.
    Type: Grant
    Filed: September 1, 2016
    Date of Patent: October 15, 2024
    Assignee: Ablynx N.V.
    Inventors: Peter Schotte, Patrick Stanssens, Christine Labeur, Jean-Luc Jonniaux, Marc Jozef Lauwereys
  • Patent number: 12116615
    Abstract: Disclosed in the present invention are a stabilizer for a color developing agent and application thereof, an application of a composition in preparation of the stabilizer, and a kit. A stabilizer for a color developing agent is provided in the present invention. The stabilizer includes a reducing substance and a weakly acidic buffer, and the weakly acidic buffer has a pH of 3.8-6.2. The reducing substance includes one or more of sodium sulfite, sodium bisulfite, sodium thiosulfate, or 1-mercaptoglycerol. The color developing agent includes one or two of a phenothiazine color developing agent or a triphenylmethane color developing agent. The color developing agent can be stably preserved by using the stabilizer. A method for stably preserving a color developing agent includes dissolving the color developing agent in the stabilizer.
    Type: Grant
    Filed: May 17, 2022
    Date of Patent: October 15, 2024
    Assignee: DA AN GENE CO., LTD.
    Inventors: Xiwen Jiang, Wenchuang Qi, Runfeng Wu, Junxing Jian, Chuanfei Mo, Yizhu Yin, Suwei Lei
  • Patent number: 12115190
    Abstract: Methods to treat cancer in a subject comprising administering to the subject a therapeutically effective amount of T-cells of the subject having increased IRF4 polypeptide expression compared to a control are disclosed. Also disclosed are methods of increasing tumor reactivity of a T-cell by increasing IRF4 polypeptide expression, and methods to predict the likelihood that a subject having cancer will respond therapeutically to administered T-cells having increased IRF4 polypeptide expression. Also disclosed are compositions comprising a T-cell and a viral vector encoding an IRF4 polypeptide. The compositions are methods are useful for treating numerous cancers in which higher level expression of IRF4 in T-cells would be beneficial. In some embodiments, activated tumor specific T-cells having increased IRF4 expression have greater infiltration in tumors and enhanced local immunological responses.
    Type: Grant
    Filed: February 27, 2019
    Date of Patent: October 15, 2024
    Assignee: THE METHODIST HOSPITAL
    Inventors: Wenhao Chen, Xian Chang Li
  • Patent number: 12104197
    Abstract: A prostaglandin production method according to the present invention comprises reacting an unsaturated fatty acid with cyclooxygenase in the presence of a reducing agent. According to the present invention, it is possible to produce prostaglandins at a high yield.
    Type: Grant
    Filed: December 25, 2020
    Date of Patent: October 1, 2024
    Assignee: Kyowa Pharma Chemical Co., Ltd.
    Inventors: Eitora Yamamura, Jun Ogawa, Akinori Ando
  • Patent number: 12104139
    Abstract: Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.
    Type: Grant
    Filed: September 6, 2019
    Date of Patent: October 1, 2024
    Assignee: DSM IP Assets B.V.
    Inventors: Neil Francis Leininger, Ginger Shank, Xiao Dong, Joseph William Pfeifer, III, Vidya Pai
  • Patent number: 12105081
    Abstract: The present invention includes methods and systems for assessing a cellular response to a treatment modality, such as potential drug candidate, by comparing normalized single-cell measurements of cellular properties without the need of a calibration step.
    Type: Grant
    Filed: April 26, 2022
    Date of Patent: October 1, 2024
    Assignee: Travera, Inc.
    Inventors: Robert Kimmerling, Selim Olcum, Mark Stevens
  • Patent number: 12098406
    Abstract: This invention provides for an improved method of stabilizing freeze dried bacterial extracts with a carbohydrate lyoprotectant such that the extracts can be for use in cell free protein synthesis. Also provided herein are formulations for stable, freeze dried bacterial extracts that when stored at room temperature retain at least about 70% protein synthesis activity compared to undried frozen bacterial extracts.
    Type: Grant
    Filed: June 29, 2022
    Date of Patent: September 24, 2024
    Assignee: Sutro Biopharma, Inc.
    Inventors: Martin Chandler, Kirk Hayenga, Henry Heinsohn
  • Patent number: 12097224
    Abstract: Disclosed is a use of a cell wall skeleton of isolated Rhodococcus ruber or a composition containing the same for preparing a human papillomavirus infection treatment drug. The cell wall skeleton of Rhodococcus ruber isolated from Rhodococcus ruber was stored and preserved at China General Microbiological Culture Collection Center, No. 1, West Beichen Road, Chaoyang District, Beijing on Mar. 22, 2019 with accession number CGMCC 17431.
    Type: Grant
    Filed: December 24, 2019
    Date of Patent: September 24, 2024
    Assignee: Liaoning Greatest Bio-Pharmaceutical Co., Ltd.
    Inventors: Bo Gai, Chunyan Dou, Yi Zhang, Guoying Zhang
  • Patent number: 12091697
    Abstract: A method for preparing glucosamine includes the steps of converting fructose-6-phosphate (F6P) and an ammonium salt to glucosamine-6-phosphate (GlcN6P) under the catalysis of glucosamine-6-phosphate deaminase (EC 3.5.99.6, GlmD); and producing glucosamine (GlcN) by the dephosphorylation of GlcN6P under the catalysis of an enzyme capable of catalyzing the dephosphorylation. Such a method can be used to prepare glucosamine by in vitro enzymatic biosystem.
    Type: Grant
    Filed: July 11, 2019
    Date of Patent: September 17, 2024
    Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES
    Inventors: Chun You, Dongdong Meng
  • Patent number: 12090173
    Abstract: Methods of treatment are provided herein, including administration of a population cells modified to enforce expression of an E-selectin and/or an L-selectin ligand, the modified cell population having a cell viability of at least 70% after a treatment to enforce such expression.
    Type: Grant
    Filed: November 10, 2022
    Date of Patent: September 17, 2024
    Assignee: THE BRIGHAM AND WOMEN'S HOSPITAL, INC.
    Inventor: Robert Sackstein
  • Patent number: 12084704
    Abstract: Described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl phosphate (DMAP) into a flavin-derived cofactor, wherein said method further comprises providing said DMAP enzymatically by: (i) the enzymatic conversion of dimethylallyl pyrophosphate (DMAPP) into said DMAP; or (ii) a single enzymatic step in which prenol is directly enzymatically converted into said DMAP; or (iii) two enzymatic steps comprising: first enzymatically converting DMAPP into prenol; and then enzymatically converting the thus obtained prenol into said DMAP; or (iv) the enzymatic conversion of isopentenyl monophosphate (IMP) into said DMAP, or by a combination of any one of (i) t
    Type: Grant
    Filed: April 19, 2018
    Date of Patent: September 10, 2024
    Assignee: Global Bioenergies
    Inventors: Romain Chayot, Mathieu Allard, Maria Anissimova
  • Patent number: 12084709
    Abstract: A method for screening substances for their ability to reduce malodours from emanations from an animal, said method comprising determining the effect of said substances on the C-S lyase activity of bacteria that emit volatile sulphuric compounds (VSCs), by contacting a test substance with a sample comprising said bacteria or a supernatant obtainable from a culture of said bacteria in the presence of a substrate for a C-S lyase, detecting the levels of thiol production from said bacteria, and comparing the results with those obtained from similar bacteria in the absence of said substance.
    Type: Grant
    Filed: February 16, 2023
    Date of Patent: September 10, 2024
    Assignee: Mars, Incorporated
    Inventors: Taichi Inui, Richard Mark Haydock
  • Patent number: 12077801
    Abstract: The present invention relates to novel process for the production of ketoisophorone via biocatalytic conversion of isophorone, in particular a one-pot biocatalytic system for conversion of ?-isophorone in a two-step oxidation process, with a first oxidation being catalyzed by a heme containing oxidoreductase such as a cytochrome P450 monooxygenase followed by another oxidation which can be either a chemical reaction or a biocatalytic reaction, in particular wherein the oxidation is catalyzed by an NAD(P) or NADP(H)-dependent oxidoreductase. The invention further provides polypeptides and nucleic acid sequences coding for cytochrome P450 monooxygenase with modified (higher) substrate selectivity, total turnover numbers and/or (re)activity compared to the wild-type enzyme. Ketoisophorone is useful as building block in the synthesis of vitamins and carotenoids.
    Type: Grant
    Filed: April 6, 2018
    Date of Patent: September 3, 2024
    Assignee: DSM IP ASSETS B.V.
    Inventors: Werner Bonrath, Martin Schürmann, Michele Tavanti, Fabio Parmeggiani, Nicholas Turner, Andrea Mattevi, Jose Ruben Casstellanos
  • Patent number: 12076376
    Abstract: The present invention provides methods of treating LAL deficiency comprising administering to a mammal a therapeutically effective amount of lysosomal acid lipase with an effective dosage frequency. Methods of improving growth and liver function, increasing LAL tissue concentration, and increasing LAL activity in a human patient suffering from LAL deficiency are also provided.
    Type: Grant
    Filed: June 30, 2022
    Date of Patent: September 3, 2024
    Assignee: Alexion Pharmaceuticals, Inc.
    Inventor: Anthony Quinn
  • Patent number: 12070038
    Abstract: A method of controlling or preventing pathogenic damage and/or pest damage in a plant propagation material, a plant, part of a plant and/or plant organ, comprising applying on the plant, part of the plant, plant organ, plant propagation material or a surrounding area thereof a phytoprotective agent comprising an enzyme and a fungicide.
    Type: Grant
    Filed: June 5, 2019
    Date of Patent: August 27, 2024
    Assignee: NOVOZYMES BIOAG A/S
    Inventors: Brian R. Scott, John William Rice, Jarrod Leland, Dave Greenshields, Sharon Inch
  • Patent number: 12071614
    Abstract: A fermentation broth which includes a microbial cell which has been subjected to a condition under which the activity an endogenous transporter for 2? fucosyllactose is increased. Also provided are methods for increasing export of 2? fucosyllactose from a microbial cell, methods for identifying an endogenous yeast transporter of 2? fucosyllactose, and microbial cells genetically engineered to increase the activity of an endogenous transporter of 2? fucosyllactose.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: August 27, 2024
    Assignee: INBIOSE N.V.
    Inventors: Lisa A. Laffend, Mark J. Nelson, Lori Ann Maggio-Hall
  • Patent number: 12071586
    Abstract: A process includes growing NRB in a nitrate reducing bacteria media to form a NRB culture and combining the NRB culture with a concentrated nitrate solution to form a NRB composition.
    Type: Grant
    Filed: February 28, 2020
    Date of Patent: August 27, 2024
    Assignee: BIOTECHNOLOGY SOLUTIONS, LLC
    Inventors: Edward Corrin, Michael Gurecki, Michael Harless