Abstract: Magnetic microcarrier beads have a particle size of 1 to 1000 micrometers and include a composite core and a polymer coating that surrounds and encapsulates the core. The composite core includes magnetic particles embedded within an indigestible polymer matrix. The coating is a digestible or indigestible polymer that facilitates cell adhesion to the surface of the beads during cell culture. Magnetic force can be used to agitate the microcarrier beads during cell culture as well as to separate the beads from cultured cells or processed bio-media.
Type:
Grant
Filed:
November 24, 2015
Date of Patent:
June 6, 2023
Assignee:
CORNING INCORPORATED
Inventors:
Stephen Joseph Caracci, Ye Fang, Ann MeeJin Ferrie, Yan Jin, Lingyan Wang, Yue Zhou
Abstract: The present invention provides a method for producing a heparosan compound having an isomerized hexuronic acid residue and a method for producing a heparan sulfate with improved C5-epimerization efficiency.
Abstract: Provided herein are methods and compositions for batch production of producer cells using fluorescence activated cell sorting (FACS). In some aspects, the disclosure provides a drug-selection-free method for batch production of producer cells using FACS. Such batch production methods and compositions can be further utilized to generate clonal populations of producer cells, e.g., for large-scale manufacturing of a polypeptide of interest.
Type:
Grant
Filed:
October 7, 2016
Date of Patent:
April 25, 2023
Assignee:
GENZYME CORPORATION
Inventors:
Victor R. Cairns, Jose Ignacio Sancho Chavida, Christine DeMaria
Abstract: The present invention provides a technique for treating the cornea. More specifically, the present invention is an agent for the treatment or prevention of a state of corneal endothelial disease, the agent including at least one factor selected from the group consisting of laminin and fragments thereof, wherein the problem is solved by also providing a technique characterized in that this agent is administered together with corneal endothelial cells. Specifically, the present invention can include laminin 511 (?5?1?1), laminin 521 (?5?2?1), or a fragment of these.
Type:
Grant
Filed:
October 30, 2015
Date of Patent:
April 25, 2023
Assignees:
KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION, THE DOSHISHA, SENJU PHARMACEUTICAL CO., LTD.
Abstract: Provided is a microorganism of the genus Epidermidibacterium belonging to the family Sporichthyaceae, or a culture thereof, or a composition for preventing, improving, or treating skin conditions or inflammatory diseases including the same.
Type:
Grant
Filed:
November 23, 2021
Date of Patent:
April 25, 2023
Assignee:
COSMAX CO., LTD.
Inventors:
Dong Geol Lee, Jin Ju Nam, Min Ji Kim, Seung Hyun Kang, Youn Joon Kim
Abstract: The present invention includes methods for preparing anticoagulant polysaccharides using several non-naturally occurring, engineered sulfotransferase enzymes that are designed to react with aryl sulfate compounds instead of the natural substrate, PAPS, to facilitate sulfo group transfer to polysaccharide sulfo group acceptors. Suitable aryl sulfate compounds include, but are not limited to, p-nitrophenyl sulfate or 4-nitrocatechol sulfate. Anticoagulant polysaccharides produced by methods of the present invention comprise N-, 3-O-, 6-O-sulfated glucosamine residues and 2-O sulfated hexuronic acid residues, have comparable anticoagulant activity compared to commercially-available anticoagulant polysaccharides, and can be utilized to form truncated anticoagulant polysaccharides having a reduced molecular weight.
Type:
Grant
Filed:
August 24, 2022
Date of Patent:
April 18, 2023
Assignee:
OPTIMVIA, LLC
Inventors:
Tarsis Ferriera Gesteira, Daniel H. Lajiness
Abstract: Disclosed is a high-throughput transcriptional assay format in Actinomycete bacteria, and Streptomyces spp. in particular, that leverages eGFP, inserted both at a neutral site and inside the biosynthetic cluster of interest, as a read-out for secondary metabolite synthesis. Using this approach, a silent gene cluster in Streptomyces albus J1074 was induced. The cytotoxins etoposide and ivermectin were revealed as potent inducers, allowing the isolation and structural characterization of nearly 20 novel small molecule products of the chosen cluster. One of these molecules is a novel antifungal, while several others inhibit a cysteine protease implicated in cancer. Studies addressing the mechanism of induction by the two elicitors led to the identification of a pathway-specific transcriptional repressor that silences the gene cluster under normal growth conditions.
Type:
Grant
Filed:
May 11, 2018
Date of Patent:
April 18, 2023
Assignee:
THE TRUSTEES OF PRINCETON UNIVERSITY
Inventors:
Mohammad R. Seyedsayamdost, Fei Xu, Kyuho Moon, Behnam Nazari
Abstract: A yeast strain producing glutathione (GSH) and aldehyde dehydrogenase, and more specifically, the yeast strains Saccharomyces cerevisiae Kwon P-1 KCTC13925BP, Saccharomyces cerevisiae Kwon P-2 KCTC14122BP, and Saccharomyces cerevisiae Kwon P-3 KCTC14123BP, which produce both glutathione and aldehyde dehydrogenase.
Abstract: An apparatus includes a housing having an aperture extending through a wall of the housing, a carbon dioxide delivery device coupled to the housing through the aperture, a non-biological skin substitute substrate, and a heater coupled to the substrate.
Type:
Grant
Filed:
February 21, 2020
Date of Patent:
March 28, 2023
Assignee:
S. C. JOHNSON & SON, INC.
Inventors:
Thomas Michael Mascari, Richard W. Wadleigh
Abstract: A food composition and pharmaceutical composition with strains of lactic acid bacteria for modulating blood glucose are provided, comprising an isolated lactic acid bacteria strain. The isolated lactic acid bacteria strain is at least one selected from a group including a GL-104 strain of Lactobacillus reuteri (CCTCC NO: M209138), an AP-32 strain of Lactobacillus salivarius subsp. salicinius (CCTCC NO: M2011127), a TYCA06 strain of Lactobacillus acidophilus (CGMCC No: 15210), and an MH-68 strain of Lactobacillus johnsonii (CCTCC NO: M2011128), or a combination thereof.
Type:
Grant
Filed:
October 11, 2019
Date of Patent:
March 28, 2023
Assignee:
GLAC BIOTECH CO., LTD.
Inventors:
Pei-Shan Hsieh, Chung-Wei Kuo, Yi-Chun Tsai, Hsieh-Hsun Ho, Yi Wei Kuo
Abstract: The present invention provides a method for evaluating effectiveness of a cell therapeutic agent. When using TGF-? and/or TSP-1 expression level(s) in: (a) a first population of transformed mammalian cells with TGF-?; and (b) a second population of untransformed mammalian cells with the same gene, respectively, as a criterion for determining effectiveness of a cell therapeutic agent, and whether or not expression thereof, it is possible to definitely determine the therapeutic efficacy of each cell therapeutic agent prior to initiation of the treatment. In addition, since use of a cell therapeutic agent without therapeutic effects is avoided, undesired procedures and side effects may not be entailed.
Type:
Grant
Filed:
June 29, 2018
Date of Patent:
March 28, 2023
Assignees:
KOLON LIFE SCIENCE, INC., KOLON TISSUEGENE, INC.
Inventors:
Su Jeong Kim, Sang Eun Noh, Jun Ho Lee, Hyeon Youl Lee, Kyoung Baek Choi, Heon Sik Choi
Abstract: An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.
Type:
Grant
Filed:
June 14, 2021
Date of Patent:
March 7, 2023
Assignee:
Fluid Quip Technologies, LLC
Inventors:
Neal Jakel, John Kwik, Michael Franko, Andrew Whalen
Abstract: The invention discloses a microfluidic device for the culture, selection and/or analysis of sample organisms such as nematodes, as well as for other biological entities such as for instance animal embryos. The device features reservoirs, culture chambers and smart filtering systems allowing for the selection of specific populations/specimens of sample organisms, thus permitting long-term cultures thereof as well as phenotypic/behavioural analyses. Systems and methods for using the microfluidic device are within the present disclosure as well.
Type:
Grant
Filed:
October 19, 2015
Date of Patent:
March 7, 2023
Assignee:
ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)
Inventors:
Matteo Cornaglia, Martin Gijs, Daniel Migliozzi
Abstract: Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
Type:
Grant
Filed:
January 26, 2019
Date of Patent:
March 7, 2023
Assignees:
Toyota Motor Corporation, Regents of the University of Minnesota
Abstract: The invention relates, in part, to methods to identify compounds to treat a phytoparasitic nematode infection and/or reduce phytoparasitic nematode contamination, and to methods and compositions to treat phytoparasitic nematode infections and to reduce phytoparasitic nematode contamination of a substrate such as, but not limited to: a plant, agricultural medium, or soil.
Type:
Grant
Filed:
January 16, 2020
Date of Patent:
February 28, 2023
Assignee:
University of New Hampshire
Inventors:
Richard H. Cote, Karyn B. Cahill, Kevin D. Schuster
Abstract: A method for screening substances for their ability to reduce malodours from emanations from an animal, said method comprising determining the effect of said substances on the C-S lyase activity of bacteria that emit volatile sulphuric compounds (VSCs), by contacting a test substance with a sample comprising said bacteria or a supernatant obtainable from a culture of said bacteria in the presence of a substrate for a C-S lyase, detecting the levels of thiol production from said bacteria, and comparing the results with those obtained from similar bacteria in the absence of said substance.
Abstract: In order to quantitatively characterize biological objects, for example individual cells, a stimulus is applied to a biological object (8) in a contactless fashion. A measurement and a further measurement are performed on the biological object (8) in order to ascertain a response of the biological object (8) to the stimulus, wherein the measurement and the further measurement comprise detecting Raman scattering on and/or in the biological object (8) and/or capturing data using digital holographic microinterferometry (DHMI). The biological object (8) is characterized according to a result of the measurement and is sorted if needed. The stimulus can be applied by means of a laser beam that creates optical tweezers or an optical trap, by means of ultrasonic waves or an electric or magnetic radio frequency field.
Abstract: The invention discloses a fermentation method for production of fucoxanthin by Nitzschia laevis, including the following steps of: step A, preparation of inocula; step B, fermentation culture: inoculating of Nitzschia laevis according to a certain volume ratio to reaction kettle containing sterile fermentation medium for aeration fermentation, preparing fucoxanthin fermentation broth through culture mean of fed-batch nutrient components; step C, obtaining high fucoxanthin induction culture solution by aeration induction culture under irradiation of monochromatic light or mixed light; extracting fucoxanthin from high fucoxanthin induction culture solution.
Abstract: System and methods for monitoring the growth of an aquatic plant culture and detecting real-time characteristics associated with the aquatic plant culture aquatic plants. The systems and methods may include a control unit configured to perform an analysis of at least one image of an aquatic plant culture. The analysis may include processing at least one collected image to determine at least one physical characteristic or state of an aquatic plant culture. Systems and methods for distributing aquatic plant cultures are also provided. The distribution systems and methods may track and control the distribution of an aquatic plant culture based on information received from various sources. Systems and methods for growing and harvesting aquatic plants in a controlled and compact environment are also provided. The systems may include a bioreactor having a plurality of vertically stacked modules designed to contain the aquatic plants and a liquid growth medium.
Abstract: Bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.