Abstract: A novel method for preparing an active protein peptide from connective tissue which includes steps of: connective tissue acquisition, segmenting, washing, pulverization, pH adjustment, enzymolysis, filteration, ultrafiltration, nanofiltration concentration, sterilization, freeze-drying, etc. The connective tissue protein peptide obtained by the method of the invention has features of high peptide content, high activity, etc., and the prepared active protein peptide of the connective tissue is easily absorbed by the human body, and has functions of preventing and/or alleviating and/or treating related diseases.

Abstract: Embodiments disclose an apparatus and methods for biological sample processing enabling isolation and enrichment of microbial or pathogenic constituents from the sample. A vessel for sample containment and extraction is further disclosed for engagement with a transducer capable of efficient sample disruption and lysis. Together these components provide a convenient and inexpensive solution for rapid sample preparation compatible with downstream analysis techniques.

Abstract: The present invention provides methods of synthesizing trehalose analogues; methods of detecting mycobacteria, and trehalose analogues.

Abstract: The present invention relates to compositions comprising probiotic and prebiotic components, mineral salts, lactoferrin, and possibly saccharomycetes, which perform correct, effective colonisation of the probiotic components administered, with enteric consequences which involve maintaining and/or restoring intestinal health and preventing the consequences of common dysbioses of the digestive tract caused by stress, incorrect dietary habits and antibiotic treatments. Said compositions also have a concomitant anti-inflammatory and immunomodulating action.

Abstract: Glucosyl Stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The glucosyl Stevia compositions were purified to >95% content of total steviol glycosides. The compositions can be used as sweetness enhancers, flavors, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

Abstract: A method and system for enhancing methane generation in biogas production includes feeding cow manure and glucose into a semi-continuous stirring tank reactor. Incubating the cow manure and glucose to form waste activated sludge with methanogen growth. The methanogen growth is enhanced by surface areas of ferrous pebbles within the semi-continuous stirring tank reactor. Feeding rice husk and water into the semi-continuous stirring tank reactor. Mixing the rice husk, the water, and the formed waste activated sludge together to form a mixture. Initiating an anaerobic digestion process during the mixing to produce high volatile fatty acids. Increasing the production of the high volatile fatty acids to a higher level by enhancing adsorption of the methanogen microorganisms onto the ferrous pebbles. Reacting the higher level of the high volatile fatty acids with the ferrous pebbles to increase conversion of the higher level of volatile fatty acids to methane while producing biogas.

Abstract: A method for optimal selection of cytotoxic agents for organ or region specific therapy that includes extracorporeal filtration of the cytotoxic agent from blood drained from the organ or region being treated and return of the filtered blood to a mammal is disclosed.

Abstract: The invention involves the provision of recombinant algal mutants that have a genetic modification to a nucleic acid sequence encoding a trehalose biosynthetic enzyme, and/or a genetic modification to a nucleic acid encoding an RNA binding domain. And in some embodiments either of these algal mutants can further have a genetic mutation to a nucleic acid sequence encoding an SGI1 polypeptide. Attenuation of one, two, or all three of these genes results in a mutant organism with increased lipid productivity. It was also discovered that one, two, three, or more genetic mutations can be accumulated or “stacked” in a particular mutant cell or organism to result in further increases in the production of lipid products. The lipid products of these mutants are useful as biofuels or for other specialty chemical products.

Abstract: The invention relates to a method for the biotechnological production of the blue-green fungus pigment xylindein from fungus biomass in a bioreactor, the reactor contents being inoculated with biomass that is uncoloured. The invention also relates to the use of uncoloured biomass of the fungus culture Chlorociboria sp. for inoculation in the biotechnological production of the blue-green fungus pigment xylindein.

Abstract: Glucosyl Stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The short-chain glucosyl Stevia compositions were purified to >95% content of total steviol glycosides. The compositions can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

Abstract: In some aspects, the disclosure relates to compositions and method for detection, classification, and treatment of cancer. In some embodiments, the disclosure relates to protease imaging sensors comprising a scaffold linked to an enzyme-specific substrate that includes a first detectable marker capable of being released from the prostate protease sensor when exposed to an enzyme present in cancer and a tumor imaging agent comprising a second detectable marker that is linked to the scaffold. In some embodiments, the disclosure relates to methods of monitor progression of a tumor in a subject based upon detection of detectable markers in a sample obtained from a subject who has been administered a protease imaging sensor, upon detection of a tumor imaging agent, or any combination thereof.

Abstract: Disclosed herein are methods, systems, and compositions for the uniform pretreatment of biomass within seconds with low inhibitor formation. The pretreatment process is used to convert biomass to a fuel, sugars, or other useful chemicals by subjecting the feedstock to a rapid retention time under pressure and temperature and/or chemical reactant. The system includes at least one high pressure, steam impermiable plug and a continuously-operating valve discharge apparatus to discharge pretreated feedstock while maintaining uniform pressure on the pretreatment system.

Abstract: The present invention provides methods for adsorbing a protein onto a conductive solid substrate, comprising the application of an external potential to the solid substrate greater than +500 mV vs. the Ag/AgCl/Cl?SAT reference electrode, in the presence of a solution comprising the protein, wherein the solution has a pH within ±2 units of the isoelectric point of the protein, and wherein the protein adsorbs to the conductive solid substrate upon application of the external potential. Some embodiments are directed to a process of protein adsorption on a solid surface mediated by an applied potential that can be affected significantly by altering a number of variables such as protein concentration, flexibility, charge, molecular weight, pH, and charge of substrate.

Abstract: The present invention concerns a new mutant strain of Clostridium acetobutylicum comprising attenuated glycerol kinase activity. In addition, the present invention concerns a consortium of Clostridium comprising at least said mutant strain and at least one other species of Clostridium chosen among C. sporogenes and C. sphenoides. As this modified strain may be adapted for growth and for the production of 1,3-propanediol in an appropriate culture medium with high glycerol content, the invention also relates to a method for the production of 1,3-propanediolandbutyric acid, by culturing at least this mutant strain in an appropriate culture medium.

Abstract: Embodiments of the present disclosure belongs to the technical field of animal feed and provides a method for rapidly evaluating metabolizable energy of goose diet by using a technique of simulative digestion gross energy. By using the technical means combining the biological method and the simulative digestion gross energy technique, metabolizable energy of goose feed can be evaluated quickly. Based on the “stomach-small intestine” two-step enzymatic methods, it is the first time to establish a regression equation between the metabolizable energy change and fiber level in the cecum to rectify the value of simulative digestion gross energy in the cecal microbial digestion phase, making the simulative digestion gross energy technique more reasonable in the assessment of metabolizable energy in geese. Results show that the use of simulative digestion gross energy technique to assess the metabolizable energy of goose feed value is highly feasible.

Abstract: The invention provides methods and reagents for detecting luciferase in biological samples. The methods and reagents of the present invention allow detecting fungal luciferase or a functional analog thereof.

Abstract: The present disclosure provides charged lipoprotein complexes that include as one component a negatively charged phospholipid that is expected to impart the complexes with improved therapeutic properties.

Abstract: The present invention provides compositions that include an extract of human feces, and methods for using such compositions, including methods for replacing or supplementing or modifying a subject's colon microbiota, and methods for treating a disease, pathological condition, and/or iatrogenic condition of the colon.

Abstract: The invention relates to a method for sample preparation on a spectrometric sample support, comprising the steps of: (i) providing the sample support with an arrangement of individual liquid droplets, for example of washing liquid or nutrient solution, each of which has microorganism sediments enclosed in it; (ii) locating a plate of an absorbent material containing cotton fibers, for example, above the sample support; (iii) lowering the plate vertically onto the sample support in such a way that microorganism sediments and plate come into contact, whereby the droplets of liquid are absorbed into the absorbent material; (iv) lifting the plate, which is locally enriched with droplet liquid, off the sample support, thereby exposing the microorganism sediments that are depleted of liquid; and (v) preparing the exposed microorganism sediments for spectrometric measurement, by means of infrared spectrometry or MALDI time-of-flight mass spectrometry, for example.

Abstract: The present invention features assays for co-culturing primary cells while maintaining key biological activities specific to the primary cells. The invention is based, at least in part, on the discovery that compositions and methods for primary cells in a high-throughput co-culture platform, image analysis for distinguishing cells in co-cultures and assays that are suitable for screening of agents in epithelial cells, such as hepatocytes.