Abstract: The present invention relates to the technical field of natural substance extraction. Disclosed is a method for extracting Citrulline from a watermelon. The method for extracting Citrulline from a watermelon in the present invention comprises Raw material pretreatment, ultrasound-enzymatic hydrolysis assisted solvent leaching, and purification steps. The purification step comprises microbial fermentation, ion-exchange resin purification, macroporous adsorption resin discoloration, and crystallization and recrystallization. The method for extracting Citrulline in the present invention is simple, requires a mild condition, and has a good extraction effect on Citrulline. The purity of Citrulline after purification is more than 90%. In addition, the Citrulline extracted by the method in the present invention meets the related health requirements and product quality standards, can be applied to food and health food industries as a raw material, and has natural security advantages.

Abstract: Described herein are methods, compositions, kits and systems for fixing biological samples. In one aspect, the method includes contacting the tissue sample with a fixing composition comprising about 20 to about 80% v/v DMSO. The solutions of the dehydration and clearing steps also include DMSO. Tissues fixed according to the methods described herein (and using the compositions herein described) may be fixed at a more rapid rate as compared to conventional procedures, but with comparable results.

Abstract: A method of sealing a durotomy from which cerebrospinal fluid is leaking. A durotomy sealant dressing is prepared by applying an active agent to a dextran base. The durotomy sealant dressing is applied to a durotomy. At least a portion of the durotomy sealant dressing dissolves. The durotomy is sealed with the dissolved durotomy sealant dressing to substantially prevent cerebrospinal fluid from flowing through the durotomy.

Abstract: A method for the treatment of vernal keratoconjunctivitis in a subject by administering to said subject an effective amount of a cell-based composition containing a suspension of mesenchymal stem cells in crystalloid with a cellular concentration from 0.01 million to 3.0 million cells/ml.

Abstract: A method for producing a biologically active, microorganism-containing aqueous solution or suspension including steps of the provision of water, the optional addition of a fine-grained mineral or organic material to the water and mixing of this materials into the water in order to obtain a suspension, the addition of compost to the suspension, and resting the suspension in an airtight environment for at least one week at a temperature of less than 15° C. This method allows the production of a biologically active, microorganism-containing solution or suspension which can be added to natural bodies of water or to soil, and may be used for external and internal application in humans and animals.

Abstract: The invention provides processes and methods for utilization of carbon dioxide (CO2) in the fermentation of a gaseous substrate comprising hydrogen (H2) and CO2. In particular, the invention allows for the conversion of at least a portion of the CO2 in the gaseous substrate to one or more products, such as ethanol, acetate, and/or 2,3-butanediol.

Abstract: The present invention provides a biomolecule conjugate having one or more functionalized biomolecules wherein the biomolecule is functionalized with one or more reactive sites, and at least one polymer capable of undergoing a polymer growth reaction, wherein the polymer is attached to at least one of the reactive sites of the functionalized biomolecule and wherein the polymer envelopes the functionalized biomolecule to form a reversible nanoparticle structure which protects the biomolecule by dynamically collapsing to preserve the biomolecule when an adverse environmental stimulus is present. A method of protecting a biomolecule from environmental conditions is also provided.

Abstract: The present disclosure provides a culture device for enumerating colonies of sulfate-reducing microorganisms. The device includes a body having a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed therebetween. The growth compartment has a perimeter and an opening. A portion of the perimeter is defined by a waterproof seal. The portion can include >50% of the perimeter. Disposed in the growth compartment are a dry cold water-soluble gelling agent, a dry culture medium selected to facilitate growth of a sulfate-reducing bacterium or indicator reagent for detecting hydrogen sulfide production by a sulfate-reducing bacterium, and a dry first oxygen-scavenging reagent.

Abstract: Use of at least one chromogenic and/or fluorogenic phosphatase substrate for the detection and/or enumeration of enterobacteria in a sample likely to contain them, such as a food sample.

Abstract: The present invention features assays for co-culturing primary cells while maintaining key biological activities specific to the primary cells. The invention is based, at least in part, on the discovery that compositions and methods for primary cells in a high-throughput co-culture platform, image analysis for distinguishing cells in co-cultures and assays that are suitable for screening of agents in epithelial cells, such as hepatocytes.

Abstract: A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

Abstract: A composition and method for generating reagents and the composition of these reagents for the stabilization and preservation of viability of cancer tissue which has been surgically excised and the suspension and/or termination of apoptosis (cell death) by significant modulation of cell metabolism by low molar concentrations of synergistic chemistries and hormonal growth enhancers while maintaining normal gene expression patterns of the surgically excised tissue.

Abstract: The present disclosure relates to a composition and methods for preparing a tissue-derived, dissolved extracellular matrix. In implementations, the extracellular matrix composition includes components native to the cornea including liquefied corneal tissue. The extracellular matrix composition can be prepared by dissolving extracted corneas in an aqueous solution of inorganic base. The resulting solution is then dialyzed to achieve a physiological pH. In some implementations, the extracellular matrix composition can be seeded and/or chemically cross-linked to form a gel matrix. The extracellular matrix and/or the gel matrix can be seeded with a desired cell type (e.g., endothelial cells, epithelial cells, stromal keratocytes, etc.) to stimulate cell proliferation and regeneration.

Abstract: A process for fermenting syngas and a fermentation medium provides high ethanol productivity while removing medium components that were previously thought to be essential. The process is effective for providing a specific STY of at least about 1 gram of ethanol/(L·day·gram cells) and for providing a selenium content in cell biomass exiting the fermentation of about 1 ppm or less.

Abstract: The invention relates to biotechnology. The plankton strain of the unicellular green alga Chlorella vulgaris GKO, which has a thin cell wall, deposited in the Russian National Collection of Industrial Microorganisms under the registration number of VKPM A1-24. The strain VKPM A1-24 of the unicellular green alga Chlorella vulgaris can be used to produce food biomass intended for preparation of a beverage, concentrate, paste or dry powder. The invention makes it possible to shorten the period of cultivation of the biomass of unicellular algae.

Abstract: The present invention relates to a composition comprising an isolated CD4+ Treg cell population, wherein the Treg cells have signatures for i) identifying that the T-cells are CD4+ regulatory Tcells, ii) identifying that the Treg cells are tissue type tropic, i.e they can migrate to the diseased tissue, iii) optionally identifying that the Treg cells are tropic with respect to the diseased tissue, i.e. they are homing cells, iv) identifying that the Treg cells are emigrant cells, i.e. they originate from the target tissue, and v) optionally identifying that the Treg cells are retained in the target tissue and optionally one or more X-signatures and/or one or more Y-signatures and one ore more Z-signatures.

Abstract: A method of inhibiting bleeding from an open surgical site includes mixing (i) a flowable gel solution comprising a biopolymer dissolved in a first solvent and (ii) a flowable hardener solution comprising a cross-linking agent dissolved in a second solvent to form a flowable hemostatic gel composition. The method also includes applying the flowable hemostatic gel composition to the open surgical site. The cross-linking agent links chains of the biopolymer together to form a solid hydrogel that inhibits bleeding from the surgical site.

Abstract: Disclosed is a postprandial glucose-measuring device for preventing the development of or reversing T2D. Included are methods for using the device as well as better use for invasive and noninvasive glucose meters. Further disclosed are novel exercise-sensitizing compositions useful for managing blood glucose levels in Type-2 diabetics with minimal risk of hypoglycemia. The disclosed glucose meters allow a user to also measure exercise and meal size—all with relatively instant feedback—more effectively than having to track the complexity posed by labels, glycemic index and calories. Also disclosed is integration of glucose-measuring devices with smartphones and health monitoring technology to make possible safe and effective interpretation of postprandial glucose readings by a patient to control or reverse Type-2 diabetes.

Abstract: The present invention relates to an improved method for drying microorganisms, especially lactic acid bacteria, in a spray dryer.

Abstract: The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film.