Abstract: Bone grafts and constructs including stem cells are provided. Example bone grafts include osteogenic stem cells seeded on a scaffold of osteoconductive cortico-cancellous chips and/or osteoinductive demineralized bone. Example constructs include extracellular matrix on a synthetic scaffold, in which the ECM is secreted from MSCs seeded onto the synthetic scaffold. Also provided are methods of making the present bone grafts and scaffolds. Further provided are methods of promoting bone healing and treating wound healing, by administering the present bone grafts and constructs to a mammal in need thereof Also provided are kits that include the present bone grafts and/or constructs, or components thereof.
Type:
Grant
Filed:
June 15, 2018
Date of Patent:
September 14, 2021
Assignee:
Globus Medical, Inc.
Inventors:
Archana Bhat, Shairali Rao, Daniel Laskowitz
Abstract: Apparatuses and kits for the detection of ATP in a liquid sample are provided. The apparatuses and kits comprise a liquid reagent composition comprising luciferin and a sampling device having a sampling portion and a handling portion. The sampling portion comprises a fibrous or a foam material and is adapted to acquire and releasably retain a predetermined volume of a liquid sample or a sample of residue from a surface. The sampling device comprises a dry coating or a liquid coating, the coating including an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. Methods of use of the apparatus or kit are also provided.
Type:
Grant
Filed:
January 28, 2019
Date of Patent:
August 10, 2021
Assignee:
3M INNOVATIVE PROPERTIES COMPANY
Inventors:
Mark B. Driscoll, Rachel E. Evans, Catherine M. Ramsay, Neil Percy
Abstract: Compositions of peritoneal dialysis solutions and metabolizing enzymes, and their uses to treat disorders associated with elevated levels of metabolites are disclosed. Animal models of hyperoxalemia are also disclosed.
Type:
Grant
Filed:
June 6, 2014
Date of Patent:
August 3, 2021
Assignee:
Allena Pharmaceuticals, Inc.
Inventors:
Alexey Margolin, Danica Grujic, Stefan Pierzynowski
Abstract: Provided are a variant of 5?-inosinic acid dehydrogenase, a microorganism including the same, and a method of preparing 5?-inosinic acid using the same.
Type:
Grant
Filed:
August 16, 2018
Date of Patent:
July 20, 2021
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Ji Hye Lee, So-jung Park, Min Ji Baek, Jin Sook Chang, Byoung Hoon Yoon
Abstract: An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production, using membrane filtration. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process) using membrane filtration, with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.
Abstract: Disclosed is a bacteria analyzing method comprising: irradiating with light a measurement sample prepared by mixing a specimen and a reagent; obtaining two types of optical information from each of at least some particles contained in the measurement sample; and generating a measurement result of the specimen with a flag representing morphological characteristics of bacteria contained in the specimen based on both of: (i) information indicative of a characteristic of a distribution pattern of particles plotted in a first region of a coordinate space including at least two axes, wherein the two types of optical information are scalable along the respective axes, and (ii) information representing a number of particles plotted in a second region being a part of the first region.
Abstract: Disclosed are a method and a reagent for quantifying HDL3 in a test sample without requiring laborious operations. The method for quantifying cholesterol in high-density lipoprotein 3 comprises reacting a test sample with one or more surfactants which react specifically with high-density lipoprotein 3, and quantifying cholesterol. When one surfactant is used, the surfactant is one selected from the group consisting of polyoxyethylene polycyclic phenyl ethers having an HLB of 12.5 to 15. When two or more surfactants are used, at least one of the surfactants is at least one selected from the group consisting of polyoxyethylene polycyclic phenyl ethers, and the two or more surfactants are combined so as to provide the total HLB of 12.5 to 15 of the combined surfactants.
Abstract: Magnetic cells plus a magnet are used to treat ED and other bodily lesions, whereby the magnetic cells are held in at or near the location of the lesion with a magnetic field for a period of time until efficacy is established. The cells can be any cell type, including stem cells, autologous cells, recombinant cells, combinations thereof and the like.
Abstract: An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.
Type:
Grant
Filed:
July 3, 2018
Date of Patent:
June 15, 2021
Assignee:
Fluid Quip Technologies, LLC
Inventors:
Neal Jakel, John Kwik, Michael Franko, Andrew Whalen
Abstract: Methods and systems for preparing clonal cell populations are described. In some instances the disclosed methods comprise: a) identifying and selecting a cell based on its position on a surface or in a container, where the selection is not based on whether the cell comprises an exogenous label or an expressed reporter; b) photoablating all non-selected cells on the surface or in the container; and c) growing a clonal population of the selected cell.
Abstract: A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.
Type:
Grant
Filed:
August 11, 2020
Date of Patent:
May 25, 2021
Assignee:
The Fynder Group, Inc.
Inventors:
Mark A. Kozubal, Richard E. Macur, Yuval C. Avniel
Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.
Abstract: Described is a method to fabricate a gold/mesoporous silica hybrid nanoparticle. According to the process, a gold nanoparticle can be conjugated onto the surface of a mesoporous silica nanoparticle to yield a photothermal stable theranostic platform, gold/mesoporous silica hybrid nanoparticle. The nanoparticles can be useful for disease detection, treatment, and monitoring.
Abstract: A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.
Type:
Grant
Filed:
August 11, 2020
Date of Patent:
May 11, 2021
Assignee:
THE FYNDER GROUP, INC.
Inventors:
Mark A. Kozubal, Richard E. Macur, Yuval C. Avniel
Abstract: A cell composition composed of spermatogonial stem cells, Sertoli cells, Leydig cells and optionally peritubular cells, is provided, as is a culture composition, artificial testicular construct, hydrogel composition, and device containing the same. A method for using the device as a physiologically relevant in vitro model of human testicular function to screen compounds for pharmacological or toxicological activity is also provided.
Type:
Grant
Filed:
October 14, 2016
Date of Patent:
May 4, 2021
Assignee:
Wake Forest University Health Sciences
Inventors:
Samuel Pendergraft, Hooman Sadri-Ardekani, Anthony Atala, Colin Bishop
Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pH less than or equal to 6.35 is formed.
Type:
Grant
Filed:
April 26, 2016
Date of Patent:
May 4, 2021
Assignee:
3M INNOVATIVE PROPERTIES COMPANY
Inventors:
Jason W. Bjork, Evan D. Brutinel, Adam J. Stanenas
Abstract: There is provided a system and method for detection of cancerous cells from an organ tissue. The system includes a plurality of cells obtained from the organ tissue, a light source for directing light through the plurality of cells and an image sensor for detecting and measuring optical transmission characteristics of the plurality of cells. The proposed method includes modeling the measured optical transmission characteristics using a statistical algorithm and calculating a figure of merit (FOM) for each of the plurality of cells for enhancing identification accuracy of cancerous cells, wherein the FOM is calculated from pole coefficients and locations corresponding to the plurality of cells.
Type:
Grant
Filed:
July 14, 2020
Date of Patent:
April 27, 2021
Assignee:
UNITED ARAB EMIRATES UNIVERSITY
Inventors:
Mahmoud Al Ahmad, Ayshathul Fouzia Abdulgani
Abstract: The present invention relates to a pharmaceutical composition for treating cancer, containing cytochrome b5 reductase 3 (CYB5R3) protein as an active ingredient. Over-expression of CYB5R3 in cancer cells results in significant reduction of hypoxia-inducible factor-1? (HIF-1?) expression, which leads inhibition of cancer cells growth in vitro and in vivo. Thus, the CYB5R3 gene or protein of the present invention can be useful as an active ingredient of a pharmaceutical composition for treating cancer.
Type:
Grant
Filed:
March 31, 2016
Date of Patent:
April 13, 2021
Inventors:
Mi Sun Won, Hyun Seung Ban, Bo Kyung Kim, Soon Woo Nam, Kyeong Lee, Young-Ju Lee, Hong-Sub Lee, Dong Uk Kim, Ki Cheol Park, Joo-Young Im, Kyoung-Jin Oh
Abstract: Methods for identifying and treating cancer patients likely to respond to topoisomerase inhibitors or likely to fail to respond to topoisomerase inhibitors are provided. The methods take advantage of the newly discovered role of BAF complexes in decatenation of DNA by topoisomerase IIa. Cancer cells are frequently at least partly defective in BAF complex activity. Such cells are targeted for therapy using certain topoisomerase IIa inhibitors according to the disclosed methods of treatment. Therapy of such cells using other topoisomerase inhibitors should be avoided.
Type:
Grant
Filed:
May 21, 2014
Date of Patent:
April 13, 2021
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Abstract: The invention relates to a method for the immobilization of biomolecules containing at least one sulfhydryl group, which method comprises contacting a modified metal surface with the biomolecule irradiating the resulting surface with UV radiation in the presence of a photo-initiator, wherein said metal surface is modified with a cross-linker compound comprising a terminal thiol or dithiol group covalently linked to the metal surface, a spacer group, which at the other terminal end is carrying an isolated double or triple bond.
Type:
Grant
Filed:
February 17, 2017
Date of Patent:
March 30, 2021
Assignee:
BOEHRINGER INGELHEIM VETMEDICA GMBH
Inventors:
Heinz Schoeder, Matthias Griessner, Frank Leenders, Ralf Kraehmer