Abstract: The invention discloses a method for producing surfactin by using Bacillus amyloliquefaciens, belonging to the technical field of industrial microorganisms. The invention synthesizes surfactin by using the screened Bacillus amyloliquefaciens CGMCC No. 12593, the yield is up to 9.43 g/L, and a fermentation process has the characteristics that the surfactin synthesis and the growth of the strain are synchronous without a lag period. The strain and the fermentation method provided by the invention will play an important role in the industrial production of surfactin and have broad application prospects.
Abstract: Disclosed is a method for producing 2?-fucosyllactose from a recombinant Corynebacterium sp. introduced with fucosyltransferase derived from Pseudopedobacter saltans. The recombinant Corynebacterium sp. microorganism introduced with fucosyltransferase derived from Pseudopedobacter saltans is capable of producing 2?-fucosyllactose at a high concentration, high yield and high productivity.
Type:
Grant
Filed:
February 14, 2019
Date of Patent:
January 4, 2022
Assignee:
ADVANCED PROTEIN TECHNOLOGIES CORP.
Inventors:
Chul Soo Shin, Jong Won Yoon, Young Ha Song, Young Sun You, Bo Mee Kim, Jin Kyung Kim, Ga Eun Lee
Abstract: The invention relates to a device and a method for growing and screening of plant samples, comprising a specialized multiwell plate system well-suited for housing granular media, for use in in vivo screening methods of uninterrupted plant tissue growth.
Abstract: Methods of inhibiting or reducing tumor metabolism and growth are disclosed. A composition containing oxygen scavenging membrane fragments is administered within a tumor to create a hypoxic environment. This interferes with tumor growth and metabolism, and can lead to tumor death.
Abstract: The disclosure discloses a method for producing long-chain glycosylated genistein and belongs to the technical fields of enzyme engineering and fermentation engineering. The disclosure provides a method for producing long-chain glycosylated genistein. By using this method to produce long-chain glycosylated genistein, the content of long-chain glycosylated genistein in a reaction solution and the ratio of the content of long-chain glycosylated genistein in the reaction solution to the content of total glycosylated genistein in the reaction solution can be increased. The content of long-chain glycosylated genistein in the reaction solution can be increased to 10.3 g/L, and the ratio of the content of long-chain glycosylated genistein in the reaction solution to the content of total glycosylated genistein in the reaction solution can be increased to 70%.
Abstract: The present invention provides a process for producing biogas and/or methane from solid spent cereal products derived from, for example, the mashing process of malt whisk(e)y and/or beer production. There is also provided a system for producing biogas and/or methane from solid spent cereal products derived from, for example, the mashing process of malt whisk(e)y and/or beer production.
Type:
Grant
Filed:
June 29, 2018
Date of Patent:
December 7, 2021
Assignee:
Blaygow Limited
Inventors:
John Morris Ross, Cornelius Martin Lynch
Abstract: A method for producing an antimicrobial agent is provided. The method includes co-fermenting at least one bacterium and at least one yeast in a growth media to produce a co-fermented product. The co-fermented product includes an antimicrobial agent. A filtration method is applied to the co-fermented product to isolate and extract the antimicrobial agent from the co-fermented product, wherein the antimicrobial agent is a secondary metabolite.
Type:
Grant
Filed:
August 12, 2019
Date of Patent:
December 7, 2021
Assignee:
Active Micro Technologies, LLC
Inventors:
Durant Scholz, Erica Segura, Maureen Danaher, Monica Beltran
Abstract: This invention pertains to the general field of microbiology, and more specifically to transfer, inoculation and/or streaking of micro-organisms, e.g. for the purpose of obtaining individual colonies. Provided is a method for streaking a microbial sample onto a solid carrier, comprising the steps of: a) contacting at least one ferromagnetic particle with a solid carrier, followed or preceded by providing the particle with at least part of said sample, and b) applying a magnetic field gradient to allow for magnetically controlled motion of said particle on said surface, such that at least part of the sample is streaked onto the solid carrier. Also provided is an apparatus for carrying out such method in an (semi-)automated fashion.
Type:
Grant
Filed:
August 1, 2018
Date of Patent:
November 30, 2021
Assignee:
BD KIESTRA B.V.
Inventors:
Trienko Marten Van Der Kaap, Martijn Kleefstra, Ingela Marie Sofie Edlund Tjernberg, Jetze Botma
Abstract: The present invention relates to organoids derived from a single cell, such as a prostate cancer cell, and methods and compositions relating to the production and use thereof, including cell culture medium for producing organoids and methods of personalized treatment for prostate cancer. The invention further provides a humanized mouse comprising a prostate organoid derived from a patient's prostate cell.
Type:
Grant
Filed:
June 19, 2015
Date of Patent:
November 23, 2021
Assignee:
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
Abstract: A method for producing a food or a precursor of the same, including the steps: (a) providing a mash or a wort or last runnings as a first nutrient medium; and (b) treatment of the first nutrient medium with lactic acid bacteria of the species Lactobacillus rossiae (DSM 15814T) or with lactic acid bacteria of at least two kinds of species including Lactobacillus rossiae (DSM 15814T). Furthermore, a corresponding food and corresponding uses are claimed.
Type:
Grant
Filed:
August 31, 2018
Date of Patent:
November 2, 2021
Inventors:
Malaika Fischer, Peter Liebert, Stefan Kreisz
Abstract: The present invention relates to method for the direct detection and/or quantification of at least one compound with a molecular weight of at least 200, wherein the compound to be detected and/or quantified is a chemically complex molecule, wherein said chemically complex molecule is substituted with at least two groups R, wherein each R group means independently —OH, —OP(O)(OH)2 or —P(O)(OH)2, with the proviso that at least two R are independently selected from —P(O)(OH)2 and —OP(O)(OH)2, wherein the compound or compounds to be detected and/or quantified are within a biological matrix, wherein said biological matrix is a biological fluid, a biological tissue, stomach contents, intestine contents, stool sample or a culture cells, wherein the method comprises performing a chromatography and identifying the retention time and/or the intensity of the signal by means of a mass or radioactivity detector.
Type:
Grant
Filed:
February 17, 2017
Date of Patent:
November 2, 2021
Assignee:
Sanifit Therapeutics S.A.
Inventors:
Joan Perello Bestard, Ciriaco Maraschiello De Zuani, Irene Lentheric, Paula Mendoza De Las Heras, Fernando Tur Espinosa, Eva Tur Tur, Maximo Encabo Alarcon, Eva Martin Becerra, Maria de Mar Benito Amengual, Bernat Isern Amengual
Abstract: The invention relates to production of a yeast strain with increased mannan oligosaccharides, amino acids, and complex carbohydrates. The invention further relates to the process to propagate and ferment such yeast. The invention further relates to methods of use of the yeast as a feed additive for animals and a food product for humans.
Abstract: Described here is a composition comprising amphiphilic silica nanoparticles, wherein the silica nanoparticles are partially fluorinated. Also described here is a method for droplet-based assay, comprising dispersing at least one aqueous droplet in a continuous fluorous phase in a microfluidic channel, wherein at least one amphiphilic silica nanoparticle is absorbed to the interface of the continuous fluorous phase and the aqueous droplet, and wherein the silica nanoparticle is partially fluorinated. Further described here is a method for droplet-based assay, comprising dispersing at least one aqueous phase droplet in a continuous fluorous phase in a microfluidic channel, wherein the continuous fluorous phase comprises at least one partially fluorinated amphiphilic particle adsorbed to an interface of the continuous fluorous phase and the aqueous phase droplet, and wherein the aqueous phase droplet comprises at least one hydrophilic polymer adsorbed to the amphiphilic particle at the interface.
Type:
Grant
Filed:
October 23, 2015
Date of Patent:
October 5, 2021
Assignees:
The Board of Trustees of the Leland Stanford Junior University, The Governors of the University of Alberta
Inventors:
Sindy Tang, Ming Pan, Fengjiao Lyu, Ratmir Derda
Abstract: Provided herein are methods and compositions for use in the treatment of depression, anxiety or a depressive or anxiety-related disorder. Embodiments describe the administration of orotic acid or a salt thereof, or the administration of one or more probiotic microorganisms, or administration of a combination of orotic acid or a salt thereof and one or more probiotic microorganisms.
Type:
Grant
Filed:
October 28, 2015
Date of Patent:
October 5, 2021
Assignee:
Medlab IP Pty Ltd
Inventors:
Luis Vitetta, Samantha Maree Coulson, Sean Hall
Abstract: Provided are pharmaceutical foam compositions comprising a peptone, a peptide hydrolysate or an enzymatically-hydrolyzed protein prepared by enzymatic hydrolysis of a full-length protein; methods of preparation and uses thereof.
Type:
Grant
Filed:
September 11, 2017
Date of Patent:
September 21, 2021
Assignees:
Omrix Biopharmaceuticals Ltd., Ethicon, Inc.
Inventors:
Tamar Auerbach-Nevo, Ashley Deanglis, Israel Nur
Abstract: Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition and then recovering the oil from the lysed cell composition. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.
Type:
Grant
Filed:
December 19, 2014
Date of Patent:
September 21, 2021
Assignee:
DSM IP Assets B.V.
Inventors:
Brock Triplett, Micah Needham, Nasrin Tabayehnejad, Ginger Shank, Kirt Lyvell Matthews, John Hogan, Mark Barker, Neil Leininger
Abstract: Bone grafts and constructs including stem cells are provided. Example bone grafts include osteogenic stem cells seeded on a scaffold of osteoconductive cortico-cancellous chips and/or osteoinductive demineralized bone. Example constructs include extracellular matrix on a synthetic scaffold, in which the ECM is secreted from MSCs seeded onto the synthetic scaffold. Also provided are methods of making the present bone grafts and scaffolds. Further provided are methods of promoting bone healing and treating wound healing, by administering the present bone grafts and constructs to a mammal in need thereof Also provided are kits that include the present bone grafts and/or constructs, or components thereof.
Type:
Grant
Filed:
June 15, 2018
Date of Patent:
September 14, 2021
Assignee:
Globus Medical, Inc.
Inventors:
Archana Bhat, Shairali Rao, Daniel Laskowitz
Abstract: Apparatuses and kits for the detection of ATP in a liquid sample are provided. The apparatuses and kits comprise a liquid reagent composition comprising luciferin and a sampling device having a sampling portion and a handling portion. The sampling portion comprises a fibrous or a foam material and is adapted to acquire and releasably retain a predetermined volume of a liquid sample or a sample of residue from a surface. The sampling device comprises a dry coating or a liquid coating, the coating including an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. Methods of use of the apparatus or kit are also provided.
Type:
Grant
Filed:
January 28, 2019
Date of Patent:
August 10, 2021
Assignee:
3M INNOVATIVE PROPERTIES COMPANY
Inventors:
Mark B. Driscoll, Rachel E. Evans, Catherine M. Ramsay, Neil Percy
Abstract: Compositions of peritoneal dialysis solutions and metabolizing enzymes, and their uses to treat disorders associated with elevated levels of metabolites are disclosed. Animal models of hyperoxalemia are also disclosed.
Type:
Grant
Filed:
June 6, 2014
Date of Patent:
August 3, 2021
Assignee:
Allena Pharmaceuticals, Inc.
Inventors:
Alexey Margolin, Danica Grujic, Stefan Pierzynowski