Patents Examined by Robert T. Crow
  • Patent number: 10889857
    Abstract: Apparatus and methods relating to DNA sequencing are provided. In one embodiment, a DNA sequencing device includes a nanochannel having a width that is approximately 0.3 nm to approximately 20 nm. A pair of electrodes having portions exposed to the nanochannel may form a tunneling current electrode (TCE) with an electrode gap of approximately 0.1 nm to approximately 2 nm, and more particularly about 0.3 nm to about 1 nm. In one embodiment, at least one of the pair of electrodes is formed as a suspended electrode. An actuator may be associated with the suspended electrode to displace it relative to the other electrode. In various embodiments, the nanochannel and/or the electrodes may be formed using thermal reflow processes to reduce the size of such features.
    Type: Grant
    Filed: February 1, 2018
    Date of Patent: January 12, 2021
    Inventors: Xiaomin Yang, ShuaiGang Xiao, David S. Kuo, Koichi Wago, Thomas Young Chang
  • Patent number: 10876150
    Abstract: Gold nanorattle probes are provided that are highly tunable, physiologically stable, and ultra-bright Raman probes for in vitro and in vivo surface-enhanced Raman scattering (SERS) applications. The nanorattles contain an essentially uniform gap between core and shell that is tunable and can range from 2 nm to 10 nm in width. This provides numerous advantages including allowing for increased loading with a variety of dye molecules that exhibit SERS in various spectral regions, including the “tissue optical window” for in vivo studies. In addition, the nanorattle probes provide an internal label when used in diagnostic methods to detect nucleic acids, proteins and other biotargets. The nanorattles have an essentially spherical gold metal nanoparticle core, a porous material of silver metal of an essentially uniform width surrounding the nanoparticle core that is loaded with one or more SERS reporter molecules, and an outer gold metal shell encapsulating the porous material.
    Type: Grant
    Filed: January 29, 2018
    Date of Patent: December 29, 2020
    Assignee: Duke University
    Inventors: Tuan Vo-Dinh, Naveen Gandra, Hoan T. Ngo
  • Patent number: 10874691
    Abstract: Provided herein are pharmaceutically acceptable sodium nitrite and pharmaceutical compositions thereof. Also provided herein are methods for determining the total non-volatile organic carbon in a sodium nitrite-containing sample. Further provided herein are methods for producing pharmaceutically acceptable sodium nitrite. Still further provided herein are methods of treatment comprising the administration of pharmaceutically acceptable sodium nitrite.
    Type: Grant
    Filed: September 23, 2019
    Date of Patent: December 29, 2020
    Assignee: Hope Medical Enterprises, Inc.
    Inventors: Craig Sherman, Anthony James Lepine, Catherine Marie Smith, Kevin Robert Wirtz, Erich Schulze
  • Patent number: 10866230
    Abstract: Provided herein are compositions, compounds, processes, and methods of use of 3D porous coating(s) on or near a nanopore(s) for analysis or detection of charged polymers such as nucleic acids, proteins, protein-nucleic acid complexes, small molecule-biological complexes, polymer-biological complexes, and/or polyelectrolytes.
    Type: Grant
    Filed: May 30, 2014
    Date of Patent: December 15, 2020
    Inventors: Mark Grinstaff, Amit Meller, Joseph Hersey, Allison Squires
  • Patent number: 10837954
    Abstract: Disclosed herein are methods and compositions for detection of target small molecules in a mixed sample by performing a competition assay between the target and a surrogate and subsequently detecting the complex types in a nanopore device.
    Type: Grant
    Filed: April 27, 2018
    Date of Patent: November 17, 2020
    Assignee: Ontera Inc.
    Inventor: Trevor J. Morin
  • Patent number: 10823721
    Abstract: In some aspects the invention is directed to methods of analyzing a polynucleotide which include steps of directing to a nanopore an excitation beam having a predetermined polarization state; translocating a polynucleotide through the nanopore, wherein nucleotides of the polynucleotide are labeled with fluorescent labels having absorption dipoles and wherein the nanopore spatially orients the fluorescent labels so that during translocation the adsorption dipoles are substantially unresponsive to the excitation beam; detecting changes in fluorescent signals generated by the fluorescent labels as nucleotides with fluorescent labels exit the nanopore and absorption dipoles thereof become responsive to excitation by the excitation beam with the predetermined polarization state; and identifying nucleotides exiting the nanopore from the changes in fluorescent signals.
    Type: Grant
    Filed: June 22, 2017
    Date of Patent: November 3, 2020
    Assignee: Quantapore, Inc.
    Inventor: Brett N. Anderson
  • Patent number: 10792246
    Abstract: Disclosed herein is a self-supporting, dissolvable, film containing dexmedetomidine or a pharmaceutically acceptable salt thereof. The film is administered orally to treat various conditions, particularly agitation, by transmucosal delivery of the active agent.
    Type: Grant
    Filed: June 26, 2019
    Date of Patent: October 6, 2020
    Inventors: Vasukumar Kakumanu, David Christian Hanley, Frank Yocca, Chetan Dalpatbhai Lathia, Scott David Barnhart
  • Patent number: 10787710
    Abstract: Disclosed herein are compositions and methods for accurately estimating the absorbed dose of radiation indicated by a subject based on the expression pattern of a panel of radiation-modulated (RM) genes at various time points following exposure of the subject to ionizing radiation.
    Type: Grant
    Filed: August 5, 2019
    Date of Patent: September 29, 2020
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Joshua LaBaer, Kristin Gillis, Garrick Wallstrom, Jin Park, Vel Murugan, Mitch Magee
  • Patent number: 10781478
    Abstract: Systems, methods and compositions of matter according to the present invention, can be used in capture/enrichment, gene expression profiling and targeted sequencing. Provided are systems, methods and compositions concerning the enhancement of nucleic acid hybridization specificity and controlling the shapes of melting curves revealed by nucleic acid hybrid pairs to optimize nucleic acid analysis. These systems, methods and compositions comprise producing a positively charged surface or surface coating, on the surface of microarray slides or other types of surfaces similarly purposed, which enhances melting curve analysis to the point of allowing detection or differentiation of small changes in sequences between nucleic acid binding partners. The accuracy or resolution of melting curve analysis was to be sufficient to distinguish between the melting of perfect matched dsDNA and dsDNA with the smallest possible change in sequence, a one base pair mismatch.
    Type: Grant
    Filed: February 15, 2019
    Date of Patent: September 22, 2020
    Inventor: Patricia Okura
  • Patent number: 10775370
    Abstract: A fluidic testing system and method for use are presented. The fluidic testing system includes a microfluidic channel, a first chamber and second chamber. The microfluidic channel has only one port for the introduction and/or extraction of fluid through the microfluidic channel. The first chamber is disposed at a terminal end of the microfluidic channel. The second chamber is coupled to the fluidic channel and is aligned such that each opening to the second chamber is configured to be aligned substantially parallel to a gravity vector during operation.
    Type: Grant
    Filed: July 14, 2016
    Date of Patent: September 15, 2020
    Inventors: Jordi Carrera Fabra, Mathias Kuphal, Rafael Bru Gilbert, Anna Comengés Casas
  • Patent number: 10765640
    Abstract: [Problem] If lidocaine is composed of non-aqueous patch, the adhesive power of the preparation tends to get lower, as the composition amount of lidocaine is higher. It is popular to solve lidocaine in dissolving agent in order to compose lidocaine in patch and release effective amount into skin. However, if the amount of dissolving agent gets higher, the adhesive power gets extremely lower, so that an long-time attachment is difficult. [Solution] A non-aqueous patch comprising lidocaine and/or its reactant, and a dissolving agent which are contained in a base of plaster, the plaster being hold by a support, of which strength of 50% stretched to longitudinal direction is less than 2,000 g/50 mm and of biaxially-oriented stretch cloth.
    Type: Grant
    Filed: September 27, 2011
    Date of Patent: September 8, 2020
    Inventors: Tatsuya Mori, Naoyuki Saida
  • Patent number: 10760182
    Abstract: A method of marking fibers, wherein the method includes providing a plurality of fibers; depositing a marker onto at least a portion of the fibers, the depositing being performed with a delivery mechanism comprising one or more outlets; and thereby marking the fibers. Also provided is a device for marking fibers, including a transport system adapted to transport fibers in a direction of a marker delivery apparatus positioned along the transport system; the delivery apparatus includes one or more outlets, adapted to deposit a solution of the marker through the outlets onto at least a portion of the fibers; and thereby marking the fibers. Authentication of a fibrous material using the marking method of the invention followed obtaining a sample of the marked fibers and assaying the sample for the presence of the nucleic acid marker; and thereby determining whether the fibrous material is authentic or counterfeit.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: September 1, 2020
    Assignee: APDN (B.V.I.) INC.
    Inventors: Alex Phidung Tran, Abdelkrim Berrada, MingHwa Benjamin Liang, Lawrence Jung
  • Patent number: 10745666
    Abstract: A therapeutic composition comprising a purified fraction of adipose-derived mesenchymal stem cells encapsulated in a three-dimensional biocompatible gel matrix, and methods, and systems for preparing and using encapsulated adipose-derived mesenchymal stem cells. Hydrogel microbeads encapsulating stem cells maintain the viability and location of the stem cells for an extended period as compared to stem cells in suspension. The gel matrix allows the release of cellular factors from the encapsulated stem cells to surrounding tissues to achieve desired therapeutic results.
    Type: Grant
    Filed: April 6, 2018
    Date of Patent: August 18, 2020
    Assignee: JointechLabs, Inc.
    Inventors: Nathan Katz, Felix Pustilnik
  • Patent number: 10741034
    Abstract: A method of marking an inventory item includes providing an activatable smoke generator and a reservoir for holding a smoke fluid and adapted to provide a flow of smoke fluid to the generator. The reservoir contains a smoke fluid including a carrier nucleic acid having a uniquely identifiable sequence, and upon activation of the smoke generator, marker smoke is generated and targeted to flow over the inventory item. The method further includes activating the smoke generator to produce the marker smoke including the carrier nucleic acid so as to cause the marker smoke to flow over the inventory item and thereby to detectably mark the inventory item with carrier nucleic acid. The invention provides methods for stably binding and immobilizing deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate.
    Type: Grant
    Filed: December 15, 2014
    Date of Patent: August 11, 2020
    Assignee: APDN (B.V.I.) INC.
    Inventors: Abdelkrim Berrada, MingHwa Benjamin Liang, Lawrence Jung
  • Patent number: 10729164
    Abstract: The present invention relates to an immuno-enhancing composition including a corn-derived polysaccharide. The immuno-enhancing composition may be a food composition, a feed composition, or a pharmaceutical composition.
    Type: Grant
    Filed: October 26, 2016
    Date of Patent: August 4, 2020
    Inventors: Kwang-Soon Shin, Sue-Jung Lee, Hye-Ryung Park, Sun Young Kim, Dong-Joo Kwon, Ho-Sung Lee
  • Patent number: 10731210
    Abstract: A DNA sequencing device and related methods, wherein the device includes a substrate, a nanochannel formed in the substrate, a first electrode positioned on a first side of the nanochannel, and a second electrode. The second electrode is positioned on a second side of the nanochannel opposite the first electrode, and is spaced apart from the first electrode to form an electrode gap that is exposed in the nanochannel. At least a portion of first electrode is movable relative to the second electrode to decrease a size of the electrode gap.
    Type: Grant
    Filed: February 1, 2018
    Date of Patent: August 4, 2020
    Inventors: Kim Yang Lee, Thomas Young Chang, David S. Kuo, ShuaiGang Xiao, Xiaomin Yang, Koichi Wago
  • Patent number: 10724031
    Abstract: The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.
    Type: Grant
    Filed: September 27, 2019
    Date of Patent: July 28, 2020
    Inventors: Han Oh Park, Jae Ha Kim, Jong Gwang Park
  • Patent number: 10709764
    Abstract: A method for treating a defect site in a living bone of an animal by applying an exogenous compound having thrombopoietic activity to the defect site in an amount effective to induce thrombopoiesis. The exogenous compound activates a thrombopoietin receptor, leading to accelerated bone formation at the defect site. Also provided is a method for repairing a segmental bone defect in an animal bone by inserting into the segmental bone defect a biodegradable bone repair scaffold that contains a compound having thrombopoietic activity. The compound activates a thrombopoietin receptor and accelerates bone formation such that bridging occurs at the segmental bone defect.
    Type: Grant
    Filed: April 23, 2019
    Date of Patent: July 14, 2020
    Assignee: Osteofuse, Inc.
    Inventors: Melissa A. Kacena, Tien-Min Gabriel Chu
  • Patent number: 10704083
    Abstract: Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.
    Type: Grant
    Filed: October 18, 2018
    Date of Patent: July 7, 2020
    Assignee: Exact Sciences Development Company, LLC
    Inventors: Michael J. Domanico, Hatim Allawi, Graham P. Lidgard, Brian Aizenstein, Oliver Hunt, Tobias Charles Zutz
  • Patent number: 10675623
    Abstract: A method includes conveying a detection solution containing a target amplicon into a detection module of a molecular diagnostic test device. The detection module includes a detection surface including a series of capture probes to which a first portion of the target amplicon is bound when the detection solution is conveyed. A first reagent formulated to produce a visible signal indicating a presence of the target amplicon is then conveyed into the detection module. The first reagent is bound to a second portion of the target amplicon when the first reagent is conveyed. A second reagent is conveyed into the detection module. The second reagent includes a precipitating substrate formulated to catalyze the production of the visible signal by producing an insoluble colored product when the second reagent is in contact with the first reagent. The method includes viewing the visible signal via a transparent portion of the detection module.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: June 9, 2020
    Assignee: Visby Medical, Inc.
    Inventors: Boris Andreyev, Rajinder K. Bhatia, Victor Briones, Phoebe Cao, Jesus Ching, Brian Ciopyk, Adam De La Zerda, Jonathan H. Hong, Helen Huang, Colin Kelly, Adrienne C. Lam, Gregory Loney, Danielle McSheery, Keith E. Moravick, Valeria Revilla, Shaunak Roy, David D. Swenson