Abstract: An example of a flow cell includes a substrate and a reaction area defined in or over the substrate. The reaction area includes two angularly offset and non-perpendicular surfaces relative to a planar surface of the substrate, a polymeric hydrogel positioned over at least a portion of each of the two angularly offset and non-perpendicular surfaces; a first primer set attached to the polymeric hydrogel that is positioned over the portion of a first of the two angularly offset and non-perpendicular surfaces; and a second primer set attached to the polymeric hydrogel that is positioned over the portion of a second of the two angularly offset and non-perpendicular surfaces, wherein the first and second primer sets are orthogonal.

Abstract: Methods for the rapid detection of the presence of variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) that contain mutations in the Spike (S) protein gene in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting SARS-CoV-2 variants containing S gene mutations and kits are provided that are designed for the detection of SARS-CoV-2 variants containing S gene mutations.

Abstract: An anti-pollution test device and a method for rapid test of nucleic acid amplifications, and a use. The anti-pollution test device includes a test portion and a mixing reaction chamber, where the mixing reaction chamber includes: a reaction chamber body, a reaction chamber cover, a channel and a mixing reaction portion. The test portion includes a test inner core, a test kit, a transparent observation window, a flow guide device, and the flow guide device including: a flow guide tube and a flow guide component. One end of the flow guide component is connected to the flow guide tube, one end of the flow guide component is connected to the test inner core, to guide a mixed solution of a hybridization solution and a sample to the test inner core. The test inner core displays a final test result in an area corresponding to the transparent observation window.

Abstract: A nucleic acid sequencing system may include a substrate coupled to a rotating disk. The substrate may include a plurality of nucleic acid samples. A detection system, including for example an objective and a camera, may detect sequencing events on the substrate while the substrate is rotated relative to the detection system around a rotational axis of the substrate, perpendicular to a surface of the substrate, by the actuation system.

Abstract: The present disclosure is directed to a nucleic acid amplification based method for determining the presence or absence of a target polynucleotide in a sample, wherein a portion of the amplified reaction mix is replaced with a further portion of the sample between pre-amplification steps to obtain a mix of an amplified reaction and an unamplified sample. The present disclosure thus provides a cumulative amplification reaction for highly sensitive detection of target polynucleotides. The present disclosure is also directed to a nucleic acid amplification reaction system for performing such a cumulative amplification reaction.

Abstract: Described herein is an aqueous, miscible, multiphase, one-pot detection system including a first phase comprising a low density solution comprising a nucleic acid detection system; and a second phase in diffusive communication with the first phase, the second phase having a higher density than the first phase, and the second phase including a nucleic acid amplification system. Also included are multiwell plates and/or devices including the system and methods of detecting target nucleic acids.

Abstract: A polymeric hydrogel is applied to a surface of a substrate including depressions separated by interstitial regions. Each depression includes a UV blocking layer positioned at a first region, and a second region that is transparent to UV and adjacent to the first region. Before or after the hydrogel is applied, a primer set is grafted to the hydrogel. The primer set includes 3? blocked uncleavable first and second pre-primers. The hydrogel or grafted layer is removed from the interstitial regions. At a first predetermined region of the grafted layer within the second region: the first pre-primers are altered to introduce cleavable first primers; and the second pre-primers are altered to introduce uncleavable second primers. At a second predetermined region of the grafted layer within the first region: the first pre-primers are altered to introduce uncleavable first primers; and the second pre-primers are blocked to introduce cleavable second primers.

Abstract: This application discloses methods of producing a DNA strand for sequencing, as well as genetic constructs, libraries, and arrays using DNA strands produced according to these methods. The application also discloses methods of sequencing using the DNA strands, genetic constructs, libraries, and arrays produced. In certain aspects, DNA being sequenced includes a target sequence and at least one adaptor sequence.

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and/or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

Abstract: Systems and methods to assess the health of various microbiomes and to identity species therein are disclosed. Described assessments and identifications can inform treatment decisions if a microbiome is determined to have a less than optimal balance of bacterial species within it; the presence of one or more negative species; and/or the absence of one or more positive species.

Abstract: The present invention relates to a use of a compound represented by formula (I) and a pharmaceutically acceptable salt thereof in the field of pharmaceutics, and in particular an application thereof in the preparation of a drug for treating pneumonia.

Abstract: The invention provides a method for validating patient-specific oligos using spike-in sequences.

Abstract: Provided are methods, devices, and systems for a loop-mediated isothermal amplification (LAMP) reactions for detecting the presence of genetic material. A LAMP device includes an enclosure having outer walls configured to define an internal space. The LAMP device also includes a tray assembly configured to support a reaction vessel configured for holding a sample for amplification. The LAMP device also includes a plurality of light emitters configured to illuminate the reaction vessel for exciting a fluorescence of the sample in the reaction vessel. The LAMP device also includes an imaging device configured to obtain an image of the sample for determining a quantity of the fluorescence in the sample. The LAMP device also includes a controller configured to regulate a temperature of the tray assembly and instruct the imaging device to obtain the image for determining the quantity of the fluorescence in the sample.

Abstract: The present invention discloses a method for rapidly detecting the total nitrogen content of the soil in an urban green land by using archaea molecular marker OTU300. The present invention provides a DNA molecule (probe), as shown in SEQ ID NO.1 of the sequence listing. The present invention also protects the application of the probe in detecting or assisting in detecting the total nitrogen content of the soil. The present invention also protects the application of the probe in comparing the total nitrogen content of the soil in different plots. Using the method provided by the present invention to detect the total nitrogen content of the soil or compare the total nitrogen content of the soil of different plots has the following advantages: small sample demand, no need for pre-treatment, short required time, low labor cost, and realizing the rapid automatic detection of large quantities of samples. The present invention deserves to apply and promote in the evaluation of soil samples.

Abstract: A method of manipulating microdroplets having an average volume in the range 0.5 femtolitres to 10 nanolitres comprised of at least one biological component and a first aqueous medium having a water activity of aw1 of less than 1 is provided. It is characterised by the step of maintaining the microdroplets in a water-immiscible carrier fluid which further includes secondary droplets having an average volume less than 25% of the average volume of the microdroplets up to and including a maximum of 4 femtolitres and wherein the volume ratio of carrier fluid to total volume of microdroplets per unit volume of the total is greater than 2:1. The method may be employed for example with microdroplets containing biological cells or with microdroplets containing single nucleoside phosphate such as are prepared in a droplet-based nucleic acid sequencer.

Abstract: Disclosed herein is a multiplex microarray having serially attached non-functionalized biomolecules attached to a polymer coating covering each electrode of an array of electrodes for assays and a method of making the multiplex microarray. The method comprises serially blocking the electrodes of the microarray with a blocking protein, electropolymerizing pyrrole or a functionalized pyrrole on the electrodes where the biomolecule is not present during polymerization, exposing the microarray to a biomolecular solution containing a non-functionalized biomolecule for attachment to the polymer coating, and then repeating the steps to form the multiplex microarray.

Abstract: Provided herein is a fluid delivery method for permeabilizing a biological sample. The method includes delivering the fluid to a first substrate and/or a second substrate. At least one of the first substrate and the second substrate includes a spacer. The method further includes assembling, subsequent to the delivering, a chamber comprising the first substrate, the second substrate, the biological sample, and the spacer. The spacer may be disposed between the first substrate and second substrate. The spacer may be configured to maintain the fluid within the chamber and maintain a separation distance between the first substrate and the second substrate. The spacer may be positioned to at least partially surround an area on the first substrate on which the biological sample is disposed and/or at least partially surround the array disposed on the second substrate.

Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.

Abstract: The present invention relates to a nasal solution used for elimination of nasal occlusions, for cleaning of the inner section of the nose and for the treatment of nasal diseases, wherein bicarbonate (HCO3?) is provided with weight proportion between 0.05% and 1.50% and carbonate (CO3?2) is provided with weight proportion between 0.05% and 1.50% as the active substance in the ingredient thereof and it has alkali pH characteristic and preferably it is obtained from alkaline, closed basin natural spring waters.

Abstract: A fluidic testing system and method for use are presented. The fluidic testing system includes a microfluidic channel, a first chamber and second chamber. The microfluidic channel has only one port for the introduction and/or extraction of fluid through the microfluidic channel. The first chamber is disposed at a terminal end of the microfluidic channel. The second chamber is coupled to the fluidic channel and is aligned such that each opening to the second chamber is configured to be aligned substantially parallel to a gravity vector during operation.