Patents Examined by Robert T. Crow
  • Patent number: 10495656
    Abstract: This invention provides biochip cartridges and instrument devices for the detection and/or analysis of target analytes from patient samples.
    Type: Grant
    Filed: July 23, 2015
    Date of Patent: December 3, 2019
    Assignee: GenMark Diagnostics, Inc.
    Inventors: Jon Faiz Kayyem, Jayashankar Srinivasan, Sean Ford
  • Patent number: 10480026
    Abstract: The invention relates to a new method of characterizing a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: November 19, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
  • Patent number: 10465244
    Abstract: This study describes about a new electrochemical biosensor using DNA-redox electrostatic interaction and their subsequent non-specific adsorption on graphene screen-printed electrode or biochip. The ruthenium hexamine molecule [Ru(NH3)6]3+, or RuHex, was observed to form complexes with free DNA in solution that adsorbed onto graphene surfaces, enabling the development of a rapid, high-sensitivity DNA biosensor. Reproducible cathodic current signals were generated from these low-cost graphene biochips, both in the presence and absence of dsDNA and loop-mediated isothermal amplification (LAMP) amplicons. The combination of the DNA-redox molecule complexes and the graphene surface therefore provided a novel detection strategy. This new biosensor was able to identify different meat species based on the isothermal amplification of target genes followed by electrochemical detection with square wave voltammetry.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: November 5, 2019
    Assignee: Universiti Brunei Darussalam
    Inventors: Sharmili Roy, Ibrahim Abdul Rahman, Minhaz Uddin Ahmed
  • Patent number: 10465184
    Abstract: The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.
    Type: Grant
    Filed: September 15, 2015
    Date of Patent: November 5, 2019
    Inventors: Han Oh Park, Jae Ha Kim, Jong Gwang Park
  • Patent number: 10456417
    Abstract: Provided herein are pharmaceutically acceptable sodium nitrite and pharmaceutical compositions thereof. Also provided herein are methods for determining the total non-volatile organic carbon in a sodium nitrite-containing sample. Further provided herein are methods for producing pharmaceutically acceptable sodium nitrite. Still further provided herein are methods of treatment comprising the administration of pharmaceutically acceptable sodium nitrite.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: October 29, 2019
    Assignee: Hope Medical Enterprises, Inc.
    Inventors: Craig Sherman, Anthony James Lepine, Catherine Marie Smith, Kevin Robert Wirtz, Erich Schulze
  • Patent number: 10457979
    Abstract: Translocation control for sensing by a nanopore, as well as methods and products related to the same, are provided. Such methods optimize duplex stability to provide high fill rate (of the hybridization sites) but do not prevent rapid dissociation required for high read rates, as well as controlling the translocation of a target molecule for sensing by a nanopore by use of a selective pulsed voltage. Products related to the same include a reporter construct comprising two or more phosphoramidites.
    Type: Grant
    Filed: May 14, 2015
    Date of Patent: October 29, 2019
    Inventors: Robert N. McRuer, Mark Stamatios Kokoris
  • Patent number: 10458978
    Abstract: The invention provides miniaturized lateral flow chromatographic and lateral flow chromatographic microarray devices (LFM). The miniaturization of lateral flow nucleic acid detection achieved by the present invention offers reduced reagent use, femtomole sensitivity, excellent linear dynamic range, and rapid detection. Moreover, the small feature sizes of capture oligonucleotides renders the potential information capacity of the platform comparable to more traditional spotted fluorescence microarrays as well as improving sensitivity. The LFM devices exemplified herein enable analytes to be detected within 10 seconds from the time of sample introduction to the LFM device. Sample volumes may be as low as about 10 microliters, significantly reducing assay costs and ameliorating reagent storage logistics. Additionally, the miniaturization of lateral flow opens the door to highly multiplexed assays, allowing many proteins or nucleic acids to be detected in a single assay.
    Type: Grant
    Filed: February 13, 2017
    Date of Patent: October 29, 2019
    Assignee: Triad National Security, LLC
    Inventor: Robert B. Cary
  • Patent number: 10448646
    Abstract: The present invention provides an attracting tool that is convenient, inexpensive, and useful for hunting. The present invention provides an attracting tool comprising an exothermic composition and an attractant, the exothermic composition containing an oxidation accelerator having an iodine adsorption number of not more than 700 g/kg, an oxidizable metal powder, and water, wherein at least the exothermic composition is housed in an air-permeable container bag.
    Type: Grant
    Filed: July 30, 2013
    Date of Patent: October 22, 2019
    Assignee: Kobayashi Pharmaceutical Co., Ltd.
    Inventor: Tetsuya Maruyama
  • Patent number: 10446768
    Abstract: Ordered (e.g., self-assembled) structures, arranged from peptide nucleic acids and/or analogs thereof, are disclosed. The peptide nucleic acids forming the ordered structures comprise from 1 to 10 PNA backbone units, at least one comprising a guanine nucleobase or an analog thereof. Processes of generating the ordered structures, uses thereof and articles-of manufacturing, devices and systems containing same are also disclosed.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: October 15, 2019
    Assignee: Ramot at Tel-Aviv University Ltd.
    Inventors: Or Berger, Lihi Adler-Abramovich, Ehud Gazit
  • Patent number: 10443089
    Abstract: The present disclosure relates to a method of multiplexing DNA sensors and optionally measuring pH in cell, a method of localizing DNA sensor in Golgi Network of scFv-Furin expressing cell and a method of identifying optimal location of fluorophore pair on the DNA sensor for multiplexing DNA sensors. The DNA sensors of the present disclosure follow independent cellular pathways and do not interact with or compromise functionality of another DNA sensor.
    Type: Grant
    Filed: February 25, 2014
    Date of Patent: October 15, 2019
    Assignee: National Centre For Biological Sciences (NCBS-TIFR)
    Inventors: Yamuna Krishnan, Souvik Modi, Sunaina Surana
  • Patent number: 10435747
    Abstract: Disclosed herein are compositions and methods for accurately estimating the absorbed dose of radiation indicated by a subject based on the expression pattern of a panel of radiation-modulated (RM) genes at various time points following exposure of the subject to ionizing radiation.
    Type: Grant
    Filed: August 11, 2015
    Date of Patent: October 8, 2019
    Inventors: Joshua LaBaer, Kristin Gillis, Garrick Wallstrom, Jin Park, Vel Murugan, Mitch Magee
  • Patent number: 10428381
    Abstract: This invention relates to the identification of modified cytosine residues, such as 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC) to be distinguished from cytosine (C) in a sample nucleotide sequence. Methods may comprise oxidizing or reducing a first portion of polynucleotides which comprise the sample nucleotide sequence; treating the oxidized or reduced first portion and a second portion of polynucleotides with bisulfite; sequencing the polynucleotides in the first and second portions of the population following steps ii) and iii) to produce first and second nucleotide sequences, respectively and; identifying the residue in the first and second nucleotide sequences which corresponds to a cytosine residue in the sample nucleotide sequence. These methods may be useful, for example in the analysis of genomic DNA and/or of RNA.
    Type: Grant
    Filed: March 21, 2016
    Date of Patent: October 1, 2019
    Assignee: Cambridge Epigenetix Limited
    Inventors: Michael John Booth, Shankar Balasubramanian
  • Patent number: 10426743
    Abstract: The present invention relates to topical pharmaceutical emulsion compositions comprising a therapeutically effective amount of 3,5-Dihydroxy-4-isopropyl-trans-stilbene or a pharmaceutically acceptable salt thereof, an oil phase, a water phase, a surfactant, and an antioxidant, and wherein the emulsion composition is homogeneous and/or the active is solubilized in the oil phase. The invention also relates to methods of treating a dermatological condition or disorder in a patient by administering the present compositions to the skin of the patient.
    Type: Grant
    Filed: January 23, 2019
    Date of Patent: October 1, 2019
    Inventors: Sujatha D. Sonti, Joey Roger Thomas, Jon Lenn, Leandro Santos, Justin Whiteman, Michael Quinn Doherty, Mary Bedard, Piyush Jain
  • Patent number: 10420842
    Abstract: Nucleic acid origami devices are provided that are non-immunogenic and/or resistant to nucleases, which may be activated by one or more external cues, such as ligands, glucose concentration or electromagnetic fields; or used for attenuation or prevention of internalization of cell-surface receptors or prolongation of shelf-life of active agents. Further provided are methods for treating diabetes and methods for preparing the devices.
    Type: Grant
    Filed: April 13, 2014
    Date of Patent: September 24, 2019
    Inventors: Ido Bachelet, Almogit Abu-Horowitz, Eldad Ben-Ishay, Yaniv Amir, Ariel Munitz, Anastasia Shapiro, Amir Shemer, Zeev Zalevsky
  • Patent number: 10415079
    Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: September 17, 2019
    Inventors: Jonathan M. Rothberg, Wolfgang Hinz
  • Patent number: 10416112
    Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or dis
    Type: Grant
    Filed: September 14, 2015
    Date of Patent: September 17, 2019
    Inventors: Chiu Tai Andrew Wong, Todd Rearick, John Donohue
  • Patent number: 10398155
    Abstract: The invention relates to a composition for admixture to animal feed or to the drinking water for animals, comprising monolaurate and monobutyrate glycerides, not comprising monoglycerides derived from a carboxylic acid having from 8 to 10 carbon atoms. The invention also relates to the use of this composition 1 as growth promoter in animal feed, preferably in poultry feed, still more preferably in broiler feed.
    Type: Grant
    Filed: November 20, 2014
    Date of Patent: September 3, 2019
    Inventors: Tina Rogge, José Vanheule
  • Patent number: 10398748
    Abstract: The present invention relates to the combination of organic acid salts with at least one active ingredient of plant origin, preferably essential oils, partially protected with vegetable fats and/or oils, which prevent these active ingredients from being digested by stomach enzymes during the digestive process. The sodium salts of short-chain acids, preferably salts of butyric, acid are preferred. The preferred essential oils are ginger, piperine, oregano, garlic, thymol, carvacrol, cinnamaldehyde and/or any combinations thereof. The combination of organic acid salts and the essential oils protected with vegetable fats and/or oils, are used as powerful promoters or stimulants for animal growth, as organic bactericides against pathogenic bacteria present in said animals and as modulators of immune response thereof.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: September 3, 2019
    Inventor: Enrique Pablos Pérez
  • Patent number: 10401266
    Abstract: In a first aspect, the invention relates to the use of a UV-polymerizable composition as an embedding medium for biological samples. The UV-polymerizable composition is a composition with a refractive index ranging from n=1.45 to n=1.6 after a polymerization process. In another aspect, the invention relates to a method for producing embedded biological samples using said UV-polymerizable composition and to the embedded biological samples themselves. The biological samples can be used in a wide variety of areas, such as diagnostics among others, and are suitable in particular for RNA-based diagnostics.
    Type: Grant
    Filed: June 19, 2015
    Date of Patent: September 3, 2019
    Assignee: Laser Zentrum Hannover e.V.
    Inventors: Mark Kuehnel, Manuela Kellner, Danny Jonigk, Nicole Izykowski, Heiko Meyer, Raoul-Amadeus Lorbeer, Marko Heidrich
  • Patent number: 10392591
    Abstract: A nucleic acid detection plate comprises a piezoelectric sensor and at least a pipe flowing through the surface of the piezoelectric sensor, two valves intervally installed on the pipe relative to the upstream end of the piezoelectric sensor, the nucleic acid to be detected is blocked in the pipe between the two valves for isothermal amplification; the nucleic acid detection system comprises the nucleic acid detection plate described above, a thermostat capable of accommodating the nucleic acid detection plate; and a signal processor capable of being date connected to the piezoelectric sensor. The inventive method simplifies device structure in through coordinated detection by combination of thermostatic amplification and piezoelectric sensing, and improves detection efficiency.
    Type: Grant
    Filed: November 2, 2015
    Date of Patent: August 27, 2019
    Inventors: Lianqun Zhou, Chuanyu Li, Jia Yao, Wei Zhang, Zhen Guo