Patents Examined by Robert T. Crow
  • Patent number: 10724031
    Abstract: The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.
    Type: Grant
    Filed: September 27, 2019
    Date of Patent: July 28, 2020
    Assignee: BIONEER CORPORATION
    Inventors: Han Oh Park, Jae Ha Kim, Jong Gwang Park
  • Patent number: 10709764
    Abstract: A method for treating a defect site in a living bone of an animal by applying an exogenous compound having thrombopoietic activity to the defect site in an amount effective to induce thrombopoiesis. The exogenous compound activates a thrombopoietin receptor, leading to accelerated bone formation at the defect site. Also provided is a method for repairing a segmental bone defect in an animal bone by inserting into the segmental bone defect a biodegradable bone repair scaffold that contains a compound having thrombopoietic activity. The compound activates a thrombopoietin receptor and accelerates bone formation such that bridging occurs at the segmental bone defect.
    Type: Grant
    Filed: April 23, 2019
    Date of Patent: July 14, 2020
    Assignee: Osteofuse, Inc.
    Inventors: Melissa A. Kacena, Tien-Min Gabriel Chu
  • Patent number: 10704083
    Abstract: Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.
    Type: Grant
    Filed: October 18, 2018
    Date of Patent: July 7, 2020
    Assignee: Exact Sciences Development Company, LLC
    Inventors: Michael J. Domanico, Hatim Allawi, Graham P. Lidgard, Brian Aizenstein, Oliver Hunt, Tobias Charles Zutz
  • Patent number: 10675623
    Abstract: A method includes conveying a detection solution containing a target amplicon into a detection module of a molecular diagnostic test device. The detection module includes a detection surface including a series of capture probes to which a first portion of the target amplicon is bound when the detection solution is conveyed. A first reagent formulated to produce a visible signal indicating a presence of the target amplicon is then conveyed into the detection module. The first reagent is bound to a second portion of the target amplicon when the first reagent is conveyed. A second reagent is conveyed into the detection module. The second reagent includes a precipitating substrate formulated to catalyze the production of the visible signal by producing an insoluble colored product when the second reagent is in contact with the first reagent. The method includes viewing the visible signal via a transparent portion of the detection module.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: June 9, 2020
    Assignee: Visby Medical, Inc.
    Inventors: Boris Andreyev, Rajinder K. Bhatia, Victor Briones, Phoebe Cao, Jesus Ching, Brian Ciopyk, Adam De La Zerda, Jonathan H. Hong, Helen Huang, Colin Kelly, Adrienne C. Lam, Gregory Loney, Danielle McSheery, Keith E. Moravick, Valeria Revilla, Shaunak Roy, David D. Swenson
  • Patent number: 10676782
    Abstract: Translocation control for sensing by a nanopore, as well as methods and products related to the same, are provided. Such methods optimize duplex stability to provide high fill rate (of the hybridization sites) but do not prevent rapid dissociation required for high read rates, as well as controlling the translocation of a target molecule for sensing by a nanopore by use of a selective pulsed voltage. Products related to the same include a reporter construct comprising two or more phosphoramidites.
    Type: Grant
    Filed: September 12, 2019
    Date of Patent: June 9, 2020
    Assignee: STRATOS GENOMICS, INC.
    Inventors: Robert N. McRuer, Mark Stamatios Kokoris
  • Patent number: 10676784
    Abstract: A sample analyzing method includes: denaturing DNA by heating a measurement specimen; bleaching the measurement specimen to inhibit autofluorescence from the measurement specimen; binding a fluorescent dye to a test substance in the measurement specimen; and capturing an image of fluorescence originated from the fluorescent dye by irradiating the measurement specimen with light. The DNA denaturation treatment is performed before the bleaching.
    Type: Grant
    Filed: March 21, 2016
    Date of Patent: June 9, 2020
    Assignee: SYSMEX CORPORATION
    Inventors: Takuya Kubo, Shigeki Iwanaga
  • Patent number: 10669581
    Abstract: The invention relates to a method for sequencing a heteropolymeric target nucleic acid sequence that involves stochastic sensing. The invention also relates to a method for improving a pore for sequencing a target nucleic acid sequence by modifying one or more sites in the pore.
    Type: Grant
    Filed: December 20, 2017
    Date of Patent: June 2, 2020
    Assignee: Oxford University Innovation Limited
    Inventors: David Jackson Stoddart, Andrew John Heron, Giovanni Maglia, John Hagan Pryce Bayley
  • Patent number: 10662471
    Abstract: This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.
    Type: Grant
    Filed: September 13, 2017
    Date of Patent: May 26, 2020
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Randall Davis, Roger Chen
  • Patent number: 10663423
    Abstract: A system for detecting electrical properties of a molecular complex is disclosed. The system includes an electrode electrically coupled to a molecular complex that outputs an electrical signal affected by an electrical property of the molecular complex, wherein the effect of the electrical property of the molecular complex on the electrical signal is characterized by an expected bandwidth. The system further includes an integrating amplifier circuit configured to receive the electrical signal from the electrode. The integrating amplifier circuit is further configured to selectively amplify and integrate a portion of the electrical signal over time within a predetermined bandwidth, wherein the predetermined bandwidth is selected at least in part based on the expected bandwidth.
    Type: Grant
    Filed: November 7, 2018
    Date of Patent: May 26, 2020
    Assignee: Roche Sequencing Solutions, Inc.
    Inventor: Roger J. A. Chen
  • Patent number: 10662465
    Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The composition comprises at least one nucleic acid sequence, formamide, and a hybridization solution, wherein the concentration of formamide is less than or equal to 25%.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: May 26, 2020
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventor: Steen Hauge Matthiesen
  • Patent number: 10655177
    Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, utilize a of cyclic and/or non-cyclic solvent that is non-toxic and may eliminate or reduce the amount of formamide in the hybridization composition.
    Type: Grant
    Filed: October 22, 2012
    Date of Patent: May 19, 2020
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventor: Steen Hauge Matthiesen
  • Patent number: 10633647
    Abstract: A method for nucleic acid isolation comprising: receiving a binding moiety solution within a process chamber; mixing the binding moiety solution with a biological sample, within the process chamber, in order to produce a moiety-sample mixture; incubating the moiety-sample mixture during a time window, thereby producing a solution comprising a set of moiety-bound nucleic acid particles and a waste volume; separating the set of moiety-bound nucleic acid particles from the waste volume; washing the set of moiety-bound nucleic acid particles; and releasing a nucleic acid sample from the set of moiety-bound nucleic acid particles. The method preferably utilizes a binding moiety comprising at least one of poly(allylamine) and polypropylenimine tetramine dendrimer, both of which reversibly bind and unbind to nucleic acids based upon environmental pH.
    Type: Grant
    Filed: November 29, 2016
    Date of Patent: April 28, 2020
    Assignee: NeuMoDx Molecular, Inc.
    Inventors: Sundaresh Brahmasandra, Michelle Mastronardi, Elizabeth Craig, Maureen Carey
  • Patent number: 10612017
    Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.
    Type: Grant
    Filed: September 4, 2018
    Date of Patent: April 7, 2020
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Wolfgang Hinz, John Leamon, David Light, Jonathan M. Rothberg
  • Patent number: 10613076
    Abstract: Optoelectronic control of solid-state nanopores and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. Methods for controlling the translocation speed of biopolymers through solid-state nanopores and methods for unblocking clogged pores by illuminating nanopores are described.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: April 7, 2020
    Assignee: THE TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Amit Meller, Nicolas Di Fiori
  • Patent number: 10610512
    Abstract: Provided herein are embodiments related to metered dose inhalers and formulations for such inhalers. In some embodiments, the inhalers are configured so as to allow a more comfortable experience for the subject receiving the formulation. In some embodiments, the formulation comprises various ingredients, such as terpenes and/or waxes, which can further enhance the level of comfort for the subject receiving the formulation.
    Type: Grant
    Filed: June 16, 2015
    Date of Patent: April 7, 2020
    Assignee: Island Breeze Systems CA, LLC
    Inventors: Michael S. Hartman, James V. C. Boyles
  • Patent number: 10590161
    Abstract: The current landscape for preparative chromatographic RNA purification uses reversed phase HPLC, but this technique presents many issues with process scale up and ion exchange for preparative purification has only been used for short RNAs. The invention provides preparative purification of RNA (e.g., mRNA) using ion (e.g., anion) exchange chromatography that allows for separation of longer RNAs up to 10,000 nucleotides in length via a scalable method. This method avoids problems with current techniques by using low pressure chromatography that is agreeable with existing equipment in cGMP commercial facilities, that uses aqueous-bases solutions as the mobile phase (rather than flammable of greater than 10 mg RNA/mL resin (e.g., using larger pore sorbents, >500 Angstroms, that display greater mRNA binding capacities), and that yields desired RNA salt forms for downstream formulation with no additional manipulation necessary (unlike ion pair reverse phase techniques).
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: March 17, 2020
    Assignee: ModernaTX, Inc.
    Inventors: William Joseph Issa, Joseph Louis Barberio, John Grant Aunins, Noubar B. Afeyan
  • Patent number: 10591476
    Abstract: Disclosed herein is a multiplex microarray having serially attached non-functionalized biomolecules attached to a polymer coating covering each electrode of an array of electrodes for assays and a method of making the multiplex microarray. The method comprises serially blocking the electrodes of the microarray with a blocking protein, electropolymerizing pyrrole or a functionalized pyrrole on the electrodes where the biomolecule is not present during polymerization, exposing the microarray to a biomolecular solution containing a non-functionalized biomolecule for attachment to the polymer coating, and then repeating the steps to form the multiplex microarray.
    Type: Grant
    Filed: March 26, 2018
    Date of Patent: March 17, 2020
    Assignee: CustomArray, Inc.
    Inventor: John Cooper
  • Patent number: 10583308
    Abstract: The invention provides methods for directing a localized biological response of a mammalian body to an implant disposed within the body. In one embodiment, a delivery system is positioned outside the body and adjacent to the implant within the body. The delivery system comprises a first tissue response modifier effective for directing a localized biological response of the body to the implant. The tissue response modifier is nonsurgically delivered from the delivery system into the body in a quantity effective to direct the localized biological response of the body to the implant. The invention also provides an implant system for long-term use comprising an implant and nonsurgical means for delivering a tissue response modifier through the epidermis of the body, the tissue response modifier effective for directing a localized biological response of the body to the implant.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: March 10, 2020
    Assignee: Profusa, Inc.
    Inventors: William A. McMillan, Natalie Wisniewski
  • Patent number: 10578523
    Abstract: A method of separating an analyte from a biological sample is described. The method comprises providing particles capable of binding said analyte when present in a solution in a container, said container comprising walls, wherein at least a part of said walls is flexible. The particles are suspended in the solution by exerting a force on the flexible part of the walls of the container more than one time. An aliquot of the suspended particles is then removed from the container. The removed aliquot is dispensed into a sample, and the sample is incubated under conditions suitable to immobilize said analyte on the particles. The particles with the bound analyte are then separated from other material and at least part of the biological sample is removed.
    Type: Grant
    Filed: November 19, 2015
    Date of Patent: March 3, 2020
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Renato Belz, Willem Mulder, Christopher Newhouse
  • Patent number: 10571460
    Abstract: This disclosure provides methods, systems, and compositions of matter for studying solvent accessibility and three-dimensional structure of biological molecules. A plasma can be used to generate marker radicals, which can interact with a biological molecule and mark the solvent-accessible portions of the biological molecule.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: February 25, 2020
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Michael R. Sussman, J. Leon Shohet, Faraz A. Choudhury, Joshua M. Blatz, Benjamin B. Minkoff, Daniel I. Benjamin