Patents Examined by Robert T. Crow
  • Patent number: 10428381
    Abstract: This invention relates to the identification of modified cytosine residues, such as 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC) to be distinguished from cytosine (C) in a sample nucleotide sequence. Methods may comprise oxidizing or reducing a first portion of polynucleotides which comprise the sample nucleotide sequence; treating the oxidized or reduced first portion and a second portion of polynucleotides with bisulfite; sequencing the polynucleotides in the first and second portions of the population following steps ii) and iii) to produce first and second nucleotide sequences, respectively and; identifying the residue in the first and second nucleotide sequences which corresponds to a cytosine residue in the sample nucleotide sequence. These methods may be useful, for example in the analysis of genomic DNA and/or of RNA.
    Type: Grant
    Filed: March 21, 2016
    Date of Patent: October 1, 2019
    Assignee: Cambridge Epigenetix Limited
    Inventors: Michael John Booth, Shankar Balasubramanian
  • Patent number: 10420842
    Abstract: Nucleic acid origami devices are provided that are non-immunogenic and/or resistant to nucleases, which may be activated by one or more external cues, such as ligands, glucose concentration or electromagnetic fields; or used for attenuation or prevention of internalization of cell-surface receptors or prolongation of shelf-life of active agents. Further provided are methods for treating diabetes and methods for preparing the devices.
    Type: Grant
    Filed: April 13, 2014
    Date of Patent: September 24, 2019
    Assignee: BAR-ILAN UNIVERSITY
    Inventors: Ido Bachelet, Almogit Abu-Horowitz, Eldad Ben-Ishay, Yaniv Amir, Ariel Munitz, Anastasia Shapiro, Amir Shemer, Zeev Zalevsky
  • Patent number: 10416112
    Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or dis
    Type: Grant
    Filed: September 14, 2015
    Date of Patent: September 17, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Chiu Tai Andrew Wong, Todd Rearick, John Donohue
  • Patent number: 10415079
    Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: September 17, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jonathan M. Rothberg, Wolfgang Hinz
  • Patent number: 10398748
    Abstract: The present invention relates to the combination of organic acid salts with at least one active ingredient of plant origin, preferably essential oils, partially protected with vegetable fats and/or oils, which prevent these active ingredients from being digested by stomach enzymes during the digestive process. The sodium salts of short-chain acids, preferably salts of butyric, acid are preferred. The preferred essential oils are ginger, piperine, oregano, garlic, thymol, carvacrol, cinnamaldehyde and/or any combinations thereof. The combination of organic acid salts and the essential oils protected with vegetable fats and/or oils, are used as powerful promoters or stimulants for animal growth, as organic bactericides against pathogenic bacteria present in said animals and as modulators of immune response thereof.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: September 3, 2019
    Assignee: NOREL ANIMAL NUTRITION USA INC.
    Inventor: Enrique Pablos Pérez
  • Patent number: 10401266
    Abstract: In a first aspect, the invention relates to the use of a UV-polymerizable composition as an embedding medium for biological samples. The UV-polymerizable composition is a composition with a refractive index ranging from n=1.45 to n=1.6 after a polymerization process. In another aspect, the invention relates to a method for producing embedded biological samples using said UV-polymerizable composition and to the embedded biological samples themselves. The biological samples can be used in a wide variety of areas, such as diagnostics among others, and are suitable in particular for RNA-based diagnostics.
    Type: Grant
    Filed: June 19, 2015
    Date of Patent: September 3, 2019
    Assignee: Laser Zentrum Hannover e.V.
    Inventors: Mark Kuehnel, Manuela Kellner, Danny Jonigk, Nicole Izykowski, Heiko Meyer, Raoul-Amadeus Lorbeer, Marko Heidrich
  • Patent number: 10398155
    Abstract: The invention relates to a composition for admixture to animal feed or to the drinking water for animals, comprising monolaurate and monobutyrate glycerides, not comprising monoglycerides derived from a carboxylic acid having from 8 to 10 carbon atoms. The invention also relates to the use of this composition 1 as growth promoter in animal feed, preferably in poultry feed, still more preferably in broiler feed.
    Type: Grant
    Filed: November 20, 2014
    Date of Patent: September 3, 2019
    Assignee: PROVIRON HOLDING N.V.
    Inventors: Tina Rogge, José Vanheule
  • Patent number: 10392658
    Abstract: The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterizing target polynucleotides using helicases.
    Type: Grant
    Filed: September 10, 2014
    Date of Patent: August 27, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Rebecca Victoria Bowen, Clive Gavin Brown, Mark Bruce, Andrew John Heron, Elizabeth Jayne Wallace, James White, Joseph Hargreaves Lloyd, David Antoni Alves, Domenico Caprotti, Lakmal Jayasinghe, Luke McNeill, John Milton, Antonino Puglisi, Szabolcs Soeroes
  • Patent number: 10392591
    Abstract: A nucleic acid detection plate comprises a piezoelectric sensor and at least a pipe flowing through the surface of the piezoelectric sensor, two valves intervally installed on the pipe relative to the upstream end of the piezoelectric sensor, the nucleic acid to be detected is blocked in the pipe between the two valves for isothermal amplification; the nucleic acid detection system comprises the nucleic acid detection plate described above, a thermostat capable of accommodating the nucleic acid detection plate; and a signal processor capable of being date connected to the piezoelectric sensor. The inventive method simplifies device structure in through coordinated detection by combination of thermostatic amplification and piezoelectric sensing, and improves detection efficiency.
    Type: Grant
    Filed: November 2, 2015
    Date of Patent: August 27, 2019
    Assignee: SUZHOU CORE SENSE NSC MEDICAL TECHNOLOGY CO., LTD
    Inventors: Lianqun Zhou, Chuanyu Li, Jia Yao, Wei Zhang, Zhen Guo
  • Patent number: 10381107
    Abstract: A nucleic acid sequencer includes: an atomically thin membrane; a solid electrode spaced apart from the atomically thin membrane and arranged in a capacitive configuration with the atomically thin membrane; a spacer member; a complementary base covalently disposed on the atomically thin membrane and arranged to base pair with a nitrogenous base of the single stranded nucleic acid; a power source in electrical communication with the solid electrode and that provides electrical power to the solid electrode; and a resistor in electrical communication with the power source and that receives electric current from the power source and that also is in electrical communication with the atomically thin membrane such that an amount of the electric current changes in response to a change in the selected distance between the atomically thin membrane and the solid electrode.
    Type: Grant
    Filed: May 18, 2017
    Date of Patent: August 13, 2019
    Assignee: GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF COMMERCE
    Inventor: Alex Smolyanitsky
  • Patent number: 10376891
    Abstract: The present invention relates to systems and methods of temperature referencing for melt curve data collection. More specifically, the present invention relates to systems and methods for collecting DNA melt curve data for a DNA sample and a temperature reference material.
    Type: Grant
    Filed: July 18, 2016
    Date of Patent: August 13, 2019
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Kenton C. Hasson, Hiroshi Inoue
  • Patent number: 10369217
    Abstract: The present invention is directed to nanoparticles comprising chitosan and an antigen, whereby the chitosan has a degree of deacetylation of about 90% and a molecular weight from 5 kDa to 80 kDa, to microparticles containing such nanoparticles as well as to a process for preparation of such particles. The particles are usable for vaccination.
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: August 6, 2019
    Assignee: MERCK PATENT GMBH
    Inventors: Andrea Hanefeld, Markus Weigandt, Michael Wolf, Percy Knolle, Matthias Schroeder, Regina Scherliess, Peter Walden, Andrea Diedrich, Hartwig Steckel, Renato Brito Baleeiro
  • Patent number: 10371604
    Abstract: In one embodiment, a method for processing a sample includes selecting a selected sample from a biological specimen, the selected sample being in contact with a first surface of a first substrate. The method also includes transferring the selected sample directly from the first surface to a container comprising an internal volume. The method also includes forming or providing a sample solution within the internal volume of the container by contacting the selected sample with a lysis mixture using a protocol. The method further includes performing an assay, experiment, or test on the sample solution while the sample solution disposed is within the internal volume of the container. In another embodiment, a method for processing a sample includes providing a selected sample comprising one or more cells. The method also includes transferring the selected sample into an internal volume of a container.
    Type: Grant
    Filed: August 10, 2016
    Date of Patent: August 6, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mousumi Rath, Mark Shannon, Jason La
  • Patent number: 10359362
    Abstract: The present invention relates to a method for manufacturing a nanoparticle array, a surface plasmon resonance-based sensor, and a method for analyzing using the same. According to one embodiment of the present invention, after a mixed solution of an ionized binder and conductive nanoparticles is prepared, a substrate is dipped into the mixed solution. Thereafter, by applying an electric field to the mixed solution into which the substrate is dipped so as to induce coating of the conductive nanoparticles on the substrate, it is possible to manufacture, by a wet method, a nanoparticle array in which the conductive nanoparticles are quickly coated on the substrate with high density.
    Type: Grant
    Filed: September 10, 2013
    Date of Patent: July 23, 2019
    Assignee: Plexense, Inc.
    Inventor: Gibum Kim
  • Patent number: 10346671
    Abstract: Compositions, films, collection devices, apparatuses, kits and methods related to biologic analyte collection and analysis include thin films of modified polycaprolactone. Methods of production and use thereof are described herein. The films, compositions, collection devices, kits and methods can be used for collection of fingerprints for both image capture and nucleic acid extraction and analysis.
    Type: Grant
    Filed: January 23, 2015
    Date of Patent: July 9, 2019
    Assignee: Diomics Corporation
    Inventors: Beverly Lynn Wolgast, John F. Steel, Thomas J. Kindt, Ryan B. Lamer
  • Patent number: 10344326
    Abstract: In an approach to magnetic flux density based DNA sequencing, a static magnetic field is provided. A chain of nucleotides is passed through the magnetic field. A change in magnetic flux density of the static magnetic field due to an ionic voltage associated with an individual nucleotide or base pair of the chain of nucleotides is measured. An identity of the nucleotide is determined based on the change in magnetic flux density.
    Type: Grant
    Filed: May 25, 2016
    Date of Patent: July 9, 2019
    Assignee: International Business Machines Corporation
    Inventors: Samuel Mathew Jawaharlal, Arunachalam Narayanan, Sathya Santhar, Balamurugaramanathan Sivaramalingam
  • Patent number: 10334853
    Abstract: Compositions suitable for topical use to treat ectoparasite infestations are described. The compositions are suitable for use on the hair of humans or animals, without the need for pharmaceuticals or medicaments. The compositions are essentially nonaqueous and non-Newtonian, such that the compositions can be controllably delivered either manually or through use of a dispensing means. One such exemplary dispenser/applicator is described. Although the compositions are otherwise substantially free of water, they exhibit good risibility characteristics and pseudoplastic rheology. Methods for the use of the nonaqueous compositions and kits containing the compositions are also presented.
    Type: Grant
    Filed: April 18, 2016
    Date of Patent: July 2, 2019
    Assignee: Larada Sciences
    Inventors: Gregory van Buskirk, Kelly J. Brodbeck
  • Patent number: 10338068
    Abstract: Provided herein are molecular automaton systems for identification, isolation, or elimination of a target biological object. Some embodiments include modules specific for a target biological object having a first biological object surface marker and a second biological object surface marker. Some embodiments include modules specific for a target biological object having a first biological object surface marker but not a second biological object surface marker.
    Type: Grant
    Filed: November 4, 2015
    Date of Patent: July 2, 2019
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Milan N. Stojanovic, Sergei Rudchenko, Maria Rudchenko, Steven Taylor
  • Patent number: 10335378
    Abstract: Pharmaceutical compositions and methods of use therefor for reducing the desire to engage in smoking are disclosed. More specifically, the pharmaceutical composition utilizes an active ingredient to reduce the side effects of smoking cessation and to limit the molecular feedback caused by nicotine-mediated activation of acetylcholine receptors.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: July 2, 2019
    Assignee: Altria Client Services LLC
    Inventors: Gerd Kobal, Justin Heynekamp, Peter Reeh, Munmaya K. Mishra, Tetyana Kichko
  • Patent number: 10329612
    Abstract: The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: June 25, 2019
    Assignee: Agena Bioscience, Inc.
    Inventor: Thomas Becker