Patents Examined by Robert T. Crow
  • Patent number: 10557855
    Abstract: Systems comprising a nanocrystal and a luminescent chromophore are disclosed herein. The luminescent chromophore can emit energy having a first wavelength. The luminescent chromophore is configured to transfer the emitted energy having a first wavelength to the nanocrystal. The luminescent chromophore can be linked to the nanocrystal via a covalent bond. Absorption of the energy having first wavelength by the nanocrystal can activate the nanocrystal and result in an increase in quantum yield. In some embodiments, the nanocrystal can include silicon, germanium, carbon, or combinations thereof. In some examples, the luminescent chromophore can be pyrene. The luminescent chromophore and the silicon containing nanocrystal can be in a ratio of about 1:1 to 100:1 in the nanocrystal system. Methods of making and using the system are also disclosed.
    Type: Grant
    Filed: August 19, 2015
    Date of Patent: February 11, 2020
    Assignees: Board of Regents, The University of Texas System, Alma Mater Studiorum—Universita di Bologna
    Inventors: Brian A. Korgel, Yixuan Yu, Paola Ceroni, Giacomo Bergamini, Mirko Locritani
  • Patent number: 10557165
    Abstract: According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.
    Type: Grant
    Filed: August 30, 2017
    Date of Patent: February 11, 2020
    Inventors: Koji Hashimoto, Keiko Ito, Mika Inada
  • Patent number: 10549281
    Abstract: A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir; (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: February 4, 2020
    Assignee: ILLUMINA, INC.
    Inventors: Dale Buermann, John A. Moon, Bryan Crane, Mark Wang, Stanley S. Hong, Jason Harris, Matthew Hage, Mark J. Nibbe
  • Patent number: 10539537
    Abstract: Sensitivity of thin film bulk acoustic resonance (TFBAR) sensors is enhanced by mass amplification and operating a high frequency.
    Type: Grant
    Filed: February 26, 2019
    Date of Patent: January 21, 2020
    Assignee: QORVO US, INC.
    Inventors: Michael Salvati, Ian Robert Harmon
  • Patent number: 10533217
    Abstract: A sample analyzing method includes: denaturing DNA by heating a measurement specimen; bleaching the measurement specimen to inhibit autofluorescence from the measurement specimen; binding a fluorescent dye to a test substance in the measurement specimen; and capturing an image of fluorescence originated from the fluorescent dye by irradiating the measurement specimen with light. The DNA denaturation treatment is performed before the bleaching.
    Type: Grant
    Filed: March 21, 2016
    Date of Patent: January 14, 2020
    Inventors: Takuya Kubo, Shigeki Iwanaga
  • Patent number: 10528790
    Abstract: Compositions, films, collection devices, apparatuses, kits and methods related to biologic analyte collection and analysis include thin films of modified polycaprolactone. Methods of production and use thereof are described herein. The films, compositions, collection devices, kits and methods can be used for collection of fingerprints for both image capture and nucleic acid extraction and analysis.
    Type: Grant
    Filed: July 2, 2019
    Date of Patent: January 7, 2020
    Assignee: Diomics Corporation
    Inventors: Beverly Lynn Wolgast, John F. Steel, Thomas J. Kindt, Ryan B. Lamer
  • Patent number: 10519434
    Abstract: Collection devices and kits for biological sample collection include a biologic sample collection device having a hydrophilic swab matrix that includes a modified polycaprolactone (PCL). Methods of production and use thereof are also described herein. The biologic sample collection devices, kits and methods described herein are used to collect a biologic sample (e.g., blood, buccal cells, etc.) and to enable extraction of nucleic acids (e.g., DNA) from that biologic sample so that the nucleic acids can be analyzed (e.g., sequencing and subsequent analyses of DNA).
    Type: Grant
    Filed: July 17, 2017
    Date of Patent: December 31, 2019
    Inventors: Jeff Morhet, Thomas Kindt, Franco Ferrari, Vasana Maneeratana, Frederic Zenhausern, Sean Grandfield, Beverly L. Wolgast, Alistair McIntyre
  • Patent number: 10512911
    Abstract: Provided herein are methods for processing and/or detecting a sample. A method can comprise providing a barrier between a first region and a second region, wherein the first region comprises the sample, wherein the barrier maintains the first region at a first atmosphere that is different than a second atmosphere of the second region, wherein a portion of the barrier comprises a fluid in coherent motion; and using a detector at least partially contained in the first region to detect one or more signals from the sample while the first region is maintained at the first atmosphere that is different than the second atmosphere of the second region. The portion of the barrier comprising fluid may have a pressure lower than the first atmosphere, the second atmosphere, or both.
    Type: Grant
    Filed: June 13, 2019
    Date of Patent: December 24, 2019
    Inventors: Nathan Beckett, Nathan Caswell
  • Patent number: 10495656
    Abstract: This invention provides biochip cartridges and instrument devices for the detection and/or analysis of target analytes from patient samples.
    Type: Grant
    Filed: July 23, 2015
    Date of Patent: December 3, 2019
    Assignee: GenMark Diagnostics, Inc.
    Inventors: Jon Faiz Kayyem, Jayashankar Srinivasan, Sean Ford
  • Patent number: 10480026
    Abstract: The invention relates to a new method of characterizing a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: November 19, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
  • Patent number: 10465184
    Abstract: The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.
    Type: Grant
    Filed: September 15, 2015
    Date of Patent: November 5, 2019
    Inventors: Han Oh Park, Jae Ha Kim, Jong Gwang Park
  • Patent number: 10465244
    Abstract: This study describes about a new electrochemical biosensor using DNA-redox electrostatic interaction and their subsequent non-specific adsorption on graphene screen-printed electrode or biochip. The ruthenium hexamine molecule [Ru(NH3)6]3+, or RuHex, was observed to form complexes with free DNA in solution that adsorbed onto graphene surfaces, enabling the development of a rapid, high-sensitivity DNA biosensor. Reproducible cathodic current signals were generated from these low-cost graphene biochips, both in the presence and absence of dsDNA and loop-mediated isothermal amplification (LAMP) amplicons. The combination of the DNA-redox molecule complexes and the graphene surface therefore provided a novel detection strategy. This new biosensor was able to identify different meat species based on the isothermal amplification of target genes followed by electrochemical detection with square wave voltammetry.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: November 5, 2019
    Assignee: Universiti Brunei Darussalam
    Inventors: Sharmili Roy, Ibrahim Abdul Rahman, Minhaz Uddin Ahmed
  • Patent number: 10457979
    Abstract: Translocation control for sensing by a nanopore, as well as methods and products related to the same, are provided. Such methods optimize duplex stability to provide high fill rate (of the hybridization sites) but do not prevent rapid dissociation required for high read rates, as well as controlling the translocation of a target molecule for sensing by a nanopore by use of a selective pulsed voltage. Products related to the same include a reporter construct comprising two or more phosphoramidites.
    Type: Grant
    Filed: May 14, 2015
    Date of Patent: October 29, 2019
    Inventors: Robert N. McRuer, Mark Stamatios Kokoris
  • Patent number: 10458978
    Abstract: The invention provides miniaturized lateral flow chromatographic and lateral flow chromatographic microarray devices (LFM). The miniaturization of lateral flow nucleic acid detection achieved by the present invention offers reduced reagent use, femtomole sensitivity, excellent linear dynamic range, and rapid detection. Moreover, the small feature sizes of capture oligonucleotides renders the potential information capacity of the platform comparable to more traditional spotted fluorescence microarrays as well as improving sensitivity. The LFM devices exemplified herein enable analytes to be detected within 10 seconds from the time of sample introduction to the LFM device. Sample volumes may be as low as about 10 microliters, significantly reducing assay costs and ameliorating reagent storage logistics. Additionally, the miniaturization of lateral flow opens the door to highly multiplexed assays, allowing many proteins or nucleic acids to be detected in a single assay.
    Type: Grant
    Filed: February 13, 2017
    Date of Patent: October 29, 2019
    Assignee: Triad National Security, LLC
    Inventor: Robert B. Cary
  • Patent number: 10456417
    Abstract: Provided herein are pharmaceutically acceptable sodium nitrite and pharmaceutical compositions thereof. Also provided herein are methods for determining the total non-volatile organic carbon in a sodium nitrite-containing sample. Further provided herein are methods for producing pharmaceutically acceptable sodium nitrite. Still further provided herein are methods of treatment comprising the administration of pharmaceutically acceptable sodium nitrite.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: October 29, 2019
    Assignee: Hope Medical Enterprises, Inc.
    Inventors: Craig Sherman, Anthony James Lepine, Catherine Marie Smith, Kevin Robert Wirtz, Erich Schulze
  • Patent number: 10448646
    Abstract: The present invention provides an attracting tool that is convenient, inexpensive, and useful for hunting. The present invention provides an attracting tool comprising an exothermic composition and an attractant, the exothermic composition containing an oxidation accelerator having an iodine adsorption number of not more than 700 g/kg, an oxidizable metal powder, and water, wherein at least the exothermic composition is housed in an air-permeable container bag.
    Type: Grant
    Filed: July 30, 2013
    Date of Patent: October 22, 2019
    Assignee: Kobayashi Pharmaceutical Co., Ltd.
    Inventor: Tetsuya Maruyama
  • Patent number: 10443089
    Abstract: The present disclosure relates to a method of multiplexing DNA sensors and optionally measuring pH in cell, a method of localizing DNA sensor in Golgi Network of scFv-Furin expressing cell and a method of identifying optimal location of fluorophore pair on the DNA sensor for multiplexing DNA sensors. The DNA sensors of the present disclosure follow independent cellular pathways and do not interact with or compromise functionality of another DNA sensor.
    Type: Grant
    Filed: February 25, 2014
    Date of Patent: October 15, 2019
    Assignee: National Centre For Biological Sciences (NCBS-TIFR)
    Inventors: Yamuna Krishnan, Souvik Modi, Sunaina Surana
  • Patent number: 10446768
    Abstract: Ordered (e.g., self-assembled) structures, arranged from peptide nucleic acids and/or analogs thereof, are disclosed. The peptide nucleic acids forming the ordered structures comprise from 1 to 10 PNA backbone units, at least one comprising a guanine nucleobase or an analog thereof. Processes of generating the ordered structures, uses thereof and articles-of manufacturing, devices and systems containing same are also disclosed.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: October 15, 2019
    Assignee: Ramot at Tel-Aviv University Ltd.
    Inventors: Or Berger, Lihi Adler-Abramovich, Ehud Gazit
  • Patent number: 10435747
    Abstract: Disclosed herein are compositions and methods for accurately estimating the absorbed dose of radiation indicated by a subject based on the expression pattern of a panel of radiation-modulated (RM) genes at various time points following exposure of the subject to ionizing radiation.
    Type: Grant
    Filed: August 11, 2015
    Date of Patent: October 8, 2019
    Inventors: Joshua LaBaer, Kristin Gillis, Garrick Wallstrom, Jin Park, Vel Murugan, Mitch Magee
  • Patent number: 10426743
    Abstract: The present invention relates to topical pharmaceutical emulsion compositions comprising a therapeutically effective amount of 3,5-Dihydroxy-4-isopropyl-trans-stilbene or a pharmaceutically acceptable salt thereof, an oil phase, a water phase, a surfactant, and an antioxidant, and wherein the emulsion composition is homogeneous and/or the active is solubilized in the oil phase. The invention also relates to methods of treating a dermatological condition or disorder in a patient by administering the present compositions to the skin of the patient.
    Type: Grant
    Filed: January 23, 2019
    Date of Patent: October 1, 2019
    Inventors: Sujatha D. Sonti, Joey Roger Thomas, Jon Lenn, Leandro Santos, Justin Whiteman, Michael Quinn Doherty, Mary Bedard, Piyush Jain